Imaging with fluorescent proteins has been revolutionary and has led to the new field of cell biology in vivo.Many new applications of this technology have been developed in recent years.Multicolor imaging technique of fluorescent protein tags get more attention,particularly the green fluorescent protein and red fluorescent protein,which has been used in many research applications.This article will make a briefly review on it.

With the development of medical technology,methods for treatment of lung cancer gradually diversified.Radiotherapy and chemotherapy have gradually become the main methods of treatment.Clinical study have found that simply adopting one kind of treatment,cannot achieve ideal control,with the result of low 5 year survival rate,and high recurrence rate and metastasis.Combining chemotherapy and radiotherapy,can achieve better effect than that by using single method of treatment,as non-small cell lung cancer (NSCLC) is particularly sensitive to the chemotherapy.Recent clinical findings indicate that treating late NSCLC patients with combined therapy methods could achieve significant clinical effect.

Eye and nervous system are anatomically and physiologically very close.About 40% of the nerve fibers in brain are correlated with visual function.Seven of twelve cranial nerves are straightly correlated with visual afferent or eye movement.Neurological diseases can affect eye in many different ways.Furthermore,systemic disorders can cause secondary neurological lesions or even primary neurological disorders,which sequentially show ocular symptoms and signs.Better understanding of the close relationship between eye and brain,together with intimate cooperation between ophthalmologists,neurologists,neurosurgeons and other specialties,are the key point to improve our neuro-ophthalmology service in China.

Objective To know the dynamic dose of individual external exposure for radiological workers in Qinghai province from 2001 to 2005. Methods Individual exposed dose for 2 733 radiological workers were measured with thermoluminescent method. Results Individual average effective dose was less than 0.53 mSv/a, 97.84% of radiological workers dose was less than 5 mSv/a. Conclusion The individual external exposure level for radiological workers is less than 5 mSv/a in Qinghai Province and it does not exceed the national standard limit.

Arsenicals ; therapeutic use ; Humans ; Leukemia, Promyelocytic, Acute ; drug therapy ; Medicine, Chinese Traditional ; Oxides ; therapeutic use

OBJECTIVETo observe the effect of conical atone needle on foot combined with Nan anvil at Yintang (CV 29) for insomnia.

METHODSSeventy patients were randomly divided into a ibian technique group and an acupuncture group,35 cases in each one. In the Sian technique group, conical stone needle on foot combined with bias anvil at Yintang(GV 29) were used,and the therapy was applied at Yongquan(KI 1), Taichong(LR 3) and Gongsun(SP 4), etc. In the acupuncture group, common acupuncture was implemented at Sanyinjiao(SP 6), Shenmen (HT 7) and Sishencong(EX-HN 1), etc. In the two groups, treatment was given for 2 weeks,once a day. There were two days to have a rest between 5-time treatment. The change of Pittsburgh sleep quality index(PSQI) before and after treatment was recorded and the clinical effect was observed after 10-time treatment and in two weeks aftet treatment.

RESULTSAfter treatment,the total scores of PSQI were declined significantly compared with those before treatment in the two groups(both P<0. 01). The total PSQI scores after treatment,the curative and effective rates and total effective rates when followed up bad no statistical significance(all P>0. 05).

CONCLUSIONConical stone needle on foot combined with Sian anvil at Yintang(GV 29) treating insomnia is safe and effective,and this treatment acquires similar efficacy with less suffering.

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adult ; Female ; Humans ; Male ; Middle Aged ; Needles ; Sleep ; Sleep Initiation and Maintenance Disorders ; physiopathology ; therapy ; Treatment Outcome

Objective:To prepare pure prokaryotically expressed protein of hyrdC and the monoclonal antibody against hyrdC,and to detect the hyrdC protein in gastric cancer with the prepared monoclonal antibody.Methods: The target DNA sequence of hyrdC was obtained by RT-PCR and subsequently inserted into prokaryotic expression vector pET32a.The TRX-hyrdC fusion protein was purified and was used to immunize 6-week-old BALB/c female mice to prepare monoclonal antibodies.The supernants of hybridoma cells were screened by enzyme-linked immunoabsorbent assay(ELISA) and identified by Western blot.The prepared monoclonal antibody was used to detect the expression of hyrdC protein in gastric cancer and adjacent normal tissues by immunohistochemical method(SP).Results: The sequence of hyrdC ORF was conformed to the reported in GenBank.Two lines of hybrids secreting monoclonal antibodies against hyrdC were established,both belonging to IgG2a subtype.Western blot showed that the prepared monoclonal antibodies specifically combined with human hyrdC protein.Immunohistochemistry(SP) results showed that the expression of hyrdC protein in gastric cancer was significantly higher than that in normal gastric mucous membrane(P

The operating principle,system structure and influencing factors for water quality are expounded and the maintenance of RO processing system is introduced.

The big trouble for invasive fungal infection(IFI)is how to define the diagnosis.Delayed therapy is the main reason of the death in the IFI patients,which underlines the importance of empiric therapy.Both cost-effectiveness ratio and patients' particular status should be considered when choosing an antifungal drug.Amphotericin B,Itraconazole,voriconazole,caspofungin and micafungin are all recommended.But there are still plenty of questions unanswered about empiric therapy,for example,how to establish th therapy based on scientific evidence.

Objective:To Construct the eukaryotic expression vectors containing four different haplotypes DNA of human CIITA gene promoter IV.Methods:Four haplotypes(CG,CC,TG and TC)can be constructed based on two single nucleotide polymorphism(SNPs)sites(G-944C and T-1350C)in promoter IV of human CIITA gene.The 487bp DNA fragments of CIITA promoter IV including the two SNPs were obtained by polymerase chain reaction(PCR)based on human genome DNA from the subjects with the CG/CG,CC/CC,TG/TG and TC/TC genotypes,which were TA cloned to pMD18-T Simple vectors and then were digested by restriction endonucleases Mlu I and Hind Ⅲ.After fragment recovery,those were ligated to four PGL3-Basic Vectors and four PGL3-Promoter Vectors respectively,which were digested by restriction endonucleases Mlu I and Hind Ⅲ as well.All recombinant plasmids were identified by sequencing.Results:Eight recombinant plasmids(CG-PGL3-Basic,CG-PGL3-Promoter,CC-PGL3-Basic,CC-PGL3-Promoter,TG-PGL3-Basic,TG-PGL3-Promoter,TC-PGL3-Basic,TC-PGL3-Promoter)containing four different haplotypes DNA of human CIITA promoter IV were obtained,whose sequences completely matched with the theoretical prediction demonstrated by sequencing.Conclusions:The eukaryotic expression vectors containing four different haplotypes DNA of human CIITA promoter IV are successfully constructed,and it lays the groundwork for the further study of different haplotype function.