Objective To investigated the relationship between serum matrix metalloproteinase-9(MMP-9) and carotid atherosclerosis(AS) in type 2 diabetes mellitus(T2DM).Methods A total of 93 patients with T2DM were recruited to our study.The intima-media thickness(IMT) and plaques of carotid artery were measured.These patients were divided into 3 groups according to their IMT values: diabetes mellitus(DM) group(n=32),carotid artery intima thicken group(n=31) and carotid artery intima plaque group(n=30).At the same time,30 healthy individuals were selected as control.Serum level of MMP-9 were determined and analyzed.Results The serum MMP-9 in DM group was significantly higher than that in healthy controls([550.26±269.28]μg/L vs.[359.70±215.62]μg/L,t=2.23,P＜0.05).The serum MMP-9 level of intima thicken group(712.15±340.47)μg/L was significantly higher than that in healthy controls(t=4.53,P＜0.01) and DM group(t=2.40,P＜0.05).The serum MMP-9 level of plaque group([889.08±247.80]μg/L) was even more significantly higher than DM group(t=4.89,P＜0.01),IMT group(t=2.53,P＜0.05) and healthy controls(t=7.01,P＜0.01).Conclusion The severity of carotid atherosclerosis in T2DM is closely associated with the serum MMP-9 level.

Objective To study the effect of short-term inte ns ive insulin treatment on the pancreatic B-cell function in type 2 diabetic mell itus (T2DM).Methods Insulin and C-peptide serum levels were measured befor e and after intensive insulin treatment in T2DM by chemical luminescence immunoa ssay.Results The levels of insulin and C-peptide were increased at 0、30、60、120、180min in OGTT in T2DM after insulin intensive treatment.Conclusion Short-term intensive insulin treatment can partiall y improve the B-cell function of T2DM with lower function and made it rest.

Objective To study the levels of serum resistin and sICAM-1 in diffferent glucose tolerance status.Methods Fasting serum resistin and sICAM-1 concentration were measured by enzyme immunoassay in 52 cases suffering from T2DM,18 cases with glucose tolerance impaired and 30 cases with normal glucose tolerance.Meanwhile,fasting blood glucose,insulin and lipids were determined.HOMA-IR was calculated.Results Fasting serum resistin concentration (ng/mL) was higher in IGT than that in those patients with T2DM and NGT (P

Using HB55 McAb, we detected HLA-DR antigens on Daudi, H18, PBL and K562 cells by dot-ELISA. Results corresponding to situation of the antigen expressions on various cell lines were obtained: colour reaction in Daudi, H18 cells and PBL was positive in different degree and K562 sample presented negative reaction. Chromatogram peaks were printed by Dual-Wavelength Flying-Spot scanner CS-9000 (SHIMADZU). Areas and high values of peaks, meaning size and colour of spot respectively, were increased with cell concentration. Both cell concentration and volume of cell suspension did not influence the diffusion of sample. An analysis for the antigen expression on cell surface and its dynamic change can be supplied by the technique.The study on quantitative assay of MHC class Ⅱ antigens on cell surface are still going on.

Objective To study the clinicopathological features of C3 glomerulonephritis and the associations between plasma complement fragments level and clinical manifestations.Methods The clinical and pathological data of the 12 patients with C3 glomerulonephritis in our division from January 1999 to June 2010 were analyzed retrospectively.Concentrations of plasma factor B,Ba,C3,C3a,C4a and C5a were detected by commercial available sandwich ELISA kits on the day of renal biopsy.Results Ten of the 12 patients were C3 glomerulonephritis without MPGN,and the rest 2 were C3 glomerulonephritis with MPGN.All the patients presented proteinuria.Two of the 10 C3 glomerulonephritis patients without MPGN presented nephrotic range proteinuria,6 with microhematuria,1 with gross hematuria,and 2 with renal insufficiency.One of the 2 C3 glomerulonephritis patients with MPGN presented nephrotic range proteinuria,accompanied by microhematuria,hypertension and renal insufficiency.The other patient showed moderate proteinuria with normal renal function.Most of C3 glomerulonephritis patients without MPGN showed mild mesangial proliferative glomerulonephritis,and 4/10 patients had various degree cresentic formation.One C3 glomerulonephritis patient with MPGN had 47.1％ cresentic formation.The concentration of plasma C3 in C3 glomerulonephritis patients was normal,while the plasma factor B was significantly decreased,and the concentrations of plasma Ba,C3a,C4a and C5a were significantly elevated.The concentration of plasma Ba was positively correlated with the proteinuria level,while the concentrations of plasma C3a,C4a and C5a were not correlated with the levels of proteinuria or plasma creatinine.Conclusions Majority of these 12 patients were C3 glomerulonephritis without MPGN,manifests as nephritic syndrome clinically and mild mesangial proliferative glomerulonephritis histopathologically.Complement activation via alternative pathway may play an important role in the pathogenesis of C3 glomerulonephritis.

Objective To observe the change of endothelium-dependent vasodilatation function and to explore its mechanism in insulin-resistant (IR) rats induced by high fat feed. Methods (1) IR rat model was established by high fat feed for 4 weeks and IR was evaluated by glucose infusion rate (GIR) of euglycemic hyperinsulinemic clamp technique. (2) Acetylcholine (Ach)-dependent vasodilation response and nitric oxide synthase (NOS)-nitric oxide (NO)-cGMP function status were observed in isolated aorta of rats. Results (1) GIR was obviously lower in high fat feed group than that in routine feed group (P

Robot-assisted surgery is becoming a widely popular technology and now entering total knee replacement. The development of total knee replacement is introduced in this paper with the emphasis on femur positioning technologies. The experiments and error analysis of robot-assisted surgical system for total knee replacement demonstrate that femur positioning technologies have a high precision and thus deserve to be popularized theoretically and practically.

Objective To investigate the use of salvianolate injection for inpatients in our hospital; To primarily evaluate its rationality and security in clinic.Methods Medical records using the salvianolate injection from December 2013 to March 2014 were surveyed statistically and analyzed in respect of patient gender and age, department, disease diagnosis, usage and dosage, solvent types, treatment course, medicine combination, occurence of ADR, etc.Results Totally 600 cases of inpatients receiving salvianolate injection were collected, about 69.33% consistent with the indication, 67.00% with a correct single dosage, and 52.50% with a correct treatment course. In the clinical application of salvianolate injection, a high rate of unreasonable usage was found in indication, single dosage, treatment course and medicine combination.ConclusionBecause the problems still existed in the use of salvianolate injection, it should be used strictly according to the medicine instructions and General Principles for Clinical Application of Traditional Chinese Medicine Injection to ensure clinical medication safety.

This study aims to analyze and improve Howland current source circuit and to study the constant current source of alternating current with high output impedance and high stability. A simulation study was carried out on the constant-current characteristics of Howland current source from two aspects of resistance match value and the selection of Op amp parameters, and then the output impedance was analyzed. The simulation experiment showed that when it was with the best matched resistance, the constant-current characteristics of Howland current source was better. Op amp parameters could affect the constant-current characteristics of Howland current source. In Howland current source circuit, the current source after parameter optimization had better output impedance and load capacity. The results showed that there was the best matched resistance in the selection by Howland current source. The Op amp with large broadband, high slew rate and open loop gain, and wide range of power supply voltage is more suitable for the bioelectrical impedance detection circuit.
Electric Impedance ; Electric Power Supplies ; Electricity

Objective To study the expressions of metadherin (MTDH) and cyclinD1 in esophageal squamous cell carcinoma (ESCC) and their clinical significances. Methods The protein expressions of MTDH and cyclinD1 were detected by immunohistochemistry in 78 cases of ESCC. Results The positive expression rate of MTDH in ESCC was 71.79%(56/78) and the positive expression rate of cyclinD1 in ESCC was 74.36%(58/78). The expressions of MTDH and cyclinD1 were significantly correlated with the degree of differentiation and lymph node metastasis (both P< 0.05), but not with the age, gender of patients and depth of tumor invasion (all P> 0.05). Conclusion The over expressions of MTDH and cyclinD1 protein may involve in the occurrence and development of esophageal carcinoma, which play important roles in the invasion and metastasis of esophageal cancer.