Objective To investigate the change of adipocyte fatty acid-binding protein(FABP4) in maternal serum and umbilical cord blood and FABP4 mRNA placental expression in patients with preeclampsia(PE). Methods A total of 60 women with PE and 60 normal pregnant women as control participated in this study. All are admitted to Fujian Maternity and Children Health Hospital for delivery from December 2008 to October 2009. Patients with PE were divided into early-onset group (n = 30, presented at ≤34 weeks of gestation) and late-onset group(n = 30, presented at > 34 weeks of gestation), with 30 normal pregnant women as early control group(≤34 weeks of gestation) and 30 as late control group(>34 weeks of gestation). Enzyme-linked immunosorbent assay (ELISA) was used to detect FABP4,fasting serum glucose,fasting insulin(FINS) in maternal serum and FABP4 in umbilical cord blood. Real-time fluorescent quantitative revere transcription PCR was used to detect placental FABP4 mRNA expression. Furthermore,clinical and biochemical parameters were recorded, such as body mass index(BMI), systolic pressure(SP),diastolic pressure (DP), mean arterial pressure (MAP), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), creatinine (Cr), uric acid (UA) , glomerular filtration rate (GFR), 24 hours urine protein in pregnant women and neonatal weight. Results (1) Maternal serum FABP4 was (176 ± 9) ng/L in early-onset PE group and (170 ± 9) ng/L in late-onset PE group, significantly elevated as compared to (81 ± 13) ng/L in early control group and (94 ± 15) ng/L in late control group. (2) Mean maternal FINS, homeostasis model of assessment for insulin resistence index (HOMA-IR) were significantly elevated in the early-onset PE group and late-onset PE group as compared to control groups, respectively. (3) Mean placental FABP4 mRNA expression were significantly elevated in the early-onset PE group and late-onset PE group as compared to late control group. However, no significant difference was found in placental FABP4 mRNA expression between early-onset and late-onset PE groups.(4) Mean umbilical cord blood FABP4 concentrations were significantly decreased in the early-onset PE group and late-onset PE group as compared to late control group. Furthermore, umbilical cord blood FABP4 concentration correlated negatively with maternal serum FABP4 level and placental FABP4 mRNA expression, but positively with neonatal weight. (5) Mean maternal serum FABP4 concentrations correlated positively with placental FABP4 mRNA expression,TG, FINS, HOMA-IR, Cr, UA; and negatively with HDL, GFR. Conclusions Increased FABP4 expression in maternal serum and placenta may be involved in the pathogenesis of preeclampsia. Increased FABP4 mRNA expression in placenta may contribute to high serum FABP4 level in women with PE.

Objective To investigate the effects of DRD1 rs265981,rs4532,rs686 and rs265976 polymorphisms on response to clozapine in resistant schizophrenic patients.Methods DRD1 genotype was determined by SNaPshot SNP technique for 154 patients with resistant schizophrenia.Clinical symptoms were evaluated by Positive and Negative Syndrome Scale (PANSS),and the responder was defined as a reduction of 50％ on PANSS score from baseline after the patients were administered orally clozapine for 8 weeks.Results The frequencies of rs265981 genotypes,alleles and rs265976 genotypes had significant differences between clozapine responder group (88 cases) and nonresponder group(66 cases) in total clinical efficacy ( x2 =10.215,P =0.004 ; x2 =4.082,P =0.041 ; x2 =14.083,P =0.007 ).The frequencies of rs265976 genotypes had significant differences between clozapine response (58 cases) and nonresponse (96 cases) to negative symptom ( x2 =9.805,P =0.046).Conclusion The polymorphisms of DRD1 gene rs265981 and rs265976 may relate with clinical response to clozapine in resistant schizophrenias.rs265981 T/T,allele T and rs265976 genotype A/A are likely to be predictive factors to the improvement of total clozapine therapeutic effects.rs265976 genotype A/A are likely to be predictive factors of negative symptom with treatment of clozapine.

The change of Dietary pattern matched to a change in diseases spectrum of Chinese people in recent years. For improving the function of clinical nutrition branch in hospitals, in accordance with the change in the spectrum and patients' characters, we need to adjust the clinical nutrition treatments individually, to enhance the health education and consultation, to set new dietary pattern for people rationally, and to carry out the scientific research in the field of the relation between concerned chemical elements of organical foods and human health status in general, in order to reach the advanced hospital standards.

Objective To descript the current situation and to analyze the impact factors of the stigma among the community women with urinary incontinence in Jinan.Methods This was a crosssectional survey by purposive sampling.506 women with urinary incontinence from 3 communities in Jinan were measured by the International Consultation on Incontinence Questionnaire-Short Form(ICIQ-SF) and the revised version of the Social Impact Scale(SIS)in order to get the information about the urinary incontinence type,severity degree and the stigma.Results The participants' total score of the SIS was (39.62±8.10) points and the score index was 55％.Score index of three subscale including social isolation,social exclusion and inner sense of shame were 58％,45％,70％; age and severity degree of urinary incontinence were independent factors and totally accounted for 14.0％ of the variance in stigma.Conclusions It suggested that,to make patients drop the cognitive errors about being incontinence,then decrease the stigma and improve the quality of life,the Health Care Sector should positively broadcast the relative knowledge of urinary incontinence and encourages patients to seek help,screen,diagnose,and get treatment earlier,and complete the tertiary prevention.

Objective:To investigate the role of reactive oxygen species (ROS) in the apoptosis of human hepatocellular carcinoma HepG_2 cells induced by PIG11 protein overexpression. Methods:The fluorescent probe 2', 7'-dichlorofluorescin diacetate (DCFH-DA) was used to label the intracellular ROS in pLXSN-PIG11-HepG_2 cells,pLXSN-HepG_2 cells and HepG_2 cells.The intracellular fluorescence intensity was detected by using the flow cytometry (FCM). The apoptotic ratio of HepG_2 was determined by FCM after elimination of intracellular ROS with 10 mmol/L N-acetylcystine (NAC). Results:The intracellular content of ROS in pLXSN-PIG11-HepG_2 cells was significantly higher than that in pLXSN-HepG_2 cells[(15.60±0.92) vs (4.90±0.70), P<0.01)]. The apoptotic ratio of pLXSN-PIG11-HepG_2 cells was significantly decreased by pretreatment with 10 mmol/L NAC (P<0.01). Conclusion:The apoptosis-inducing effect of PIG11 over-expression is related with elevation of intracellular ROS levels.

Requirement for Medical English teaching in physical medicine physicians training has been on the agenda to fit the new condition of globalization. According to the development of physical medicine in China and students English level, courses of medical English were set to match the requirements of both scientific research and clinic works. We try to improve students' medical English level through lectures in multimedia classroom and a lot of practical activities after class.

Objective To provide scientific evidence for nutrition therapy and health education for patients with type 2 diabetes mellitus (T2DM).Methods Based on body mass index (BMI),75 newly admitted T2DM patients were assigned to the normal body-weight group and the over-weight group.Three days before admission,their dietary status was investigated.The average energy intake was compared with the average standard supply.The energy from protein,fat or carbohydrate and the intake of fiber were compared with the recommendations from the American Diabetes Association (ADA).The intake of other nutrient was compared with the recommended nutrient intakes (RNI) or adequate intakes (AI).Results Compared with the standard supply,the average energy intake of the normal body-weight group was significantly decreased (t =2.61,P ＜ 0.05 ),however the average energy intake of the overweight group was significantly increased (t =3.91,P＜0.05).The percentage of energy from protein of the two groups was significantly higher than target levels ( t values were 13.23,21.13 respectively; all P ＜ 0.05 ) ; the percentage of energy from protein of the two groups was significantly higher than target levels (t values were 5.36,10.66 respectively ; all P ＜ 0.05 ) ; however,the percentage of energy from carbohydrate was lower in both groups ( t values were 6.94,15.76 respectively; all P ＜ 0.05 ) ; the average intake of fiber were also lower in both groups (t values were 26.54,40.80 respectively ; all P ＜ 0.05 ).ConclusionThe participants showed insufficient knowledge on healthy diet.Health education could play a role in balanced diet and energy intake.

BACKGROUND:Under mitomycin C treatment, feeder cells appear to have restricted proliferation, but they are stil able to secret different cytokines. Non-mesenchymal stem cells from the bone marrow and secreted factors in plasma maintain the micro-environment suitable for the growth of mesenchymal stem cells that can improve the yield of mesenchymal stem cells.
OBJECTIVE:To study the biological characteristics of C57 mouse bone marrow mesenchymal stem cells isolated using a whole bone marrow adherent culture technique.
METHODS:Using the whole bone marrow adherent culture technique, purified and amplified C57 mouse bone marrow mesenchymal stem cells were harvested. cellproliferation kinetics, immune cellsurface markers, multiple differentiation potential and cellcycle were detected.
RESULTS AND CONCLUSION:Using the whole bone marrow culture, mouse bone marrow mesenchymal stem cells were harvested and capable of adhering to the plastic culture vessel. The obtained cells expressed CD45, CD105 and Sca-1, but were negative for CD34, CD33 and C-kit. The doubling time was (57.11±1.5) hours. The cells could be induced to differentiate into osteoblasts, adipocytes and chondrocytes. The cellcycle analysis showed that 64%of cells were in G 0-G 1 phase. These indicates that C57 mouse bone marrow mesenchymal stem cells isolated using a whole bone marrow adherent culture technique have biological characteristics of mesenchymal stem cells.

BACKGROUND:In vitro isolation and purity technique of stem cells mostly depends on the identification of cell surface marker,such as monoclonal antibody adherent spreading method,flow cell sorting method and immunomagnetic beads sorting method,but the operation was complicated and the price was high.OBJECTIVE:To observe the biological characteristics of human amniotic fluid-derived embryonic mesenchymal stem cells,which were isolated and cultured using the two-step method.DESIGN,TIME AND SETTING:The opening study was conducted at the Stem Cell Research Room of Xinjiang Medical University from March 2008 to March 2009.MATERIALS:Totally 10 amniotic fluid specimens were obtained from pregnant women who underwent prenatal diagnosis following 16-22 weeks of gestation or voluntarily induced abortion.With ultrasonic guidance,amniocentesis was performed to collect 20-40 mL amniotic fluid.METHODS:Human amniotic fluid-derived embryonic mesenchymal stem cells were isolated and cultured using the two-step method.Amniotic fluid was first centrifuged and incubated till spindle-shape cells were seen,with the presence of flbroblast-tike cell colonies.Supematant was moved to a new 25 cm~2 culture flask for further culture till spindle-shape fibroblast-like mesenchymal stem cell colonies.When 70% confluence,cells were digested,and incubated in α-MEM,supplemented with basic fibroblast growth factor,served as the first passage.MAIN OUTCOME MEASURES:Morphological changes in human amniotic fluid-derived embryonic mesenchymal stem cells of primary culture and subculture were measured.Karyotype,cycle,growth curve and colony formation ability of human amniotic fluid-derived embryonic mesenchymal stem cells were measured.Surface antigen and cytokine were examined using flow cytometry,immunofluorescence and RT-PCR.RESULTS:Human amniotic fluid-derived embryonic mesenchymal stem cells were successfully isolated and subcultured.During metaphase,primarily cultured amniotic fluid cells presented scattered spindle cells and flbroblast-like mesenchymal stem cell colonies every 7 days.Passaged cells completely adhered in 12 hours.Following 1 or 2 days of latent period,cells proliferated rapidly.About 90% confluence was observed following 6 or 7 days of culture.Cell arranged regularly,showing whirlpool-shape,radiated shape.Cells were spindle-shape,with unclear boundary.Chromosome karyotype of human amniotic fluid-derived embryonic mesenchymal stem cells was normal diploid.Growth curve showed "S" shape,but the two-step method reached a peak at (6.1±0.5) days,which was significantly rapid compared with the one-step method (7.2±0.6) days (P=0.035).Flow cytometry analyses showed that P3 cells at S phase took up (14±2.3)% using the two-step method,which was more than the one-step method (9.0±1.4)% (P=0.031).Low-density human amniotic fluid-derived embryonic mesenchymal stem cells were incubated for 7 days prior to cells formed scattered cell colonies.However,colony forming efficiency using the two-step method (15.0±2.3)% were significantly more than the one-step method (10.0±1.8)% (P=0.021).Flow cytometry results showed that human amniotic fluid-derived embryonic mesenchymal stem cells expressed CD44,CD29 and CD105,but were negatively for CD45,CD34,HLA-DR.Immunofluorescence suggested that Oct-4-positive cells were observed in amniotic fluid.However,the proportion of Oct-4-positive cells using two-step method (1.2±0.3)% was significantly greater than the one-step method (0.9±0.2)% (P=0.041).RT-PCR suggested that human amniotic fluid-derived embryonic mesenchymal stem cells obtained using the two methods expressed Oct-4.CONCLUSION:Human multipotent mesenchymal stem cells are present in human amniotic fluid.The two-step culture protocol could be a kind of high performance and simple protocol which may not interfere with the normal prenatal diagnosis procedure.

Bone marrow mesenchymal stem cells (BMSCs) have been shown to be multipotent cells that possess high self-replicating capacity. The purpose of our study was to investigate the feasibility of using enteric neuron-like cells obtained by in vitro induction and differentiated from rat BMSCs for the treatment of Hirschsprung's disease (HD). Glial cell-derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) are neurotrophic factors that play important roles in neuronal development, differentiation, survival and function. Meanwhile, GDNF mutations are a major cause of HD. In this study, BMSCs were transfected with eukaryotic expression plasmids co-expressing GDNF and NT-3, and the transfected cells displayed neuron-like changes after differentiation induced by fetal gut culture medium (FGCM). Immunofluorescence assay showed positive expression of the neuronal marker NSE and the enteric neuronal markers PGP9.5, VIP and nNOS. Reverse transcription-polymerase chain reaction (RT-PCR) revealed the expression of GDNF and NT-3 in transfected BMSCs. The present study indicates that genetically modified BMSCs co-expressing GDNF and NT-3 are able to differentiate into enteric neuronal cells and express enteric nerve markers when induced by FGCM. This study provides an experimental basis for gene therapy to treat enteric nervous system-related disorders, such as HD.