Objective: To explore the clinical research on the treatment of osteoporosis with calcitonin and Low frequency pulse electromagnetic field. Methods:Selected 84 cases of primary osteoporosis were randomly divided into control group and treatment group, 42 cases in each group, two groups were given calcium, vitamin D and other drugs conventional treatment, on the basis of the control group, intramuscular injections salmon calcitonin in the treatment group and intramuscular injection of salmon calcitonin with PEMF therapy, before treatment and after 3 and 6 months, respectively. Results: Two groups of patients after treatment, the pain was significantly reduced compared with before treatment, the longer the treatment, the more obvious decline;Treatment of three months, six months later, the treatment group compared with the control group bone mineral density were significant differences(t=3.129, t=6.457;P<0.05);The two groups no serious adverse reactions. Conclusion:Osteoporosis patients in the conventional therapy treatment based on intramuscular injection of salmon calcitonin and with PEMF treatment can significantly reduce the patient's pain, increase bone density, efficacy, adverse reactions, with some clinical reference significance.

The main intention of this paper is to propose suggestions for optimizing China 's high-value medical consumables centralized procurement .In this paper , we have analyzed the developed countries 'best practices in high-value medical consumables procurement from the perspectives of procurement principle ( s), key procurement bodies, procurement patterns, supervision, and management.The results of our analysis show that “Value for mon-ey” is the core principle in procurement and hospital route , national/regional centralized route ( s) , and group pur-chase organization ( GPO) route are four primary procurement patterns in foreign countries .Procurement management is optimized through improving product coding system and encouraging health technology assessment ( HTA) and clin-ical practitioners'involvement in procurement decision-making.In that perspective, it's been suggested that China's high-value medical consumables centralized procurement could be improved from three aspects:referring partial HTA indicators so as to enhance tender evaluation and realize value-based purchasing;establishing a standard and unified coding system for medical devices;introducing GPO route and make market surveillance mechanism into full play .

Understanding inter-species transmission of influenza viruses is an important research topic. Scientists try to identify and evaluate the functional factors determining the host range of influenza viruses by generating the recombinant viruses through reverse genetics in laboratories, which reveals the viruses' molecular mechanisms of infection and transmission in different species. Therefore, the reverse genetic method is a very important tool for further understanding the biology of influenza viruses and will provide the insight for the prevention and treatment of infections and transmission. However, these recombinant influenza viruses generated in laboratories will become the potential threat to the public health and the environment. In this paper, we discussed the biological safety issues of recombinant influenza viruses and suggested we should set up protocols for risk management on research activities related to recombinant highly pathogenic influenza viruses.
Influenza A Virus, H5N1 Subtype ; genetics ; Laboratories ; Microbiology ; Orthomyxoviridae ; genetics ; Public Health ; Reassortant Viruses ; genetics ; Recombination, Genetic ; Safety

OBJECTIVE: To investigate the characteristics of psychiatric comorbidity in pharyngitis patients. METHOD: The psychological investigation and evaluation were done in 100 pharyngitis patients by using self-reporting inventory 90 (SCL-90). All the results were compared with healthy Chinese population, including factors of gender, age, course of disease and education level, using rank-sum test analysis, SPSS 19.0. RESULT: Mild and moderate levels of psychosis were respectively noted in 65% and 14%, with high score of somatization (P < 0.05), obsession (P < 0.05), interpersonal sensitivity (P < 0.05), anxiety (P < 0.05) and other (sleep, diet, P < 0.05) compared with control group. The factor scores of obsession (P < 0.05), anxiety (P < 0.05) in male group were higher than female group. The factor scores of somatization (P < 0.05) and obsession (P < 0.05) in junior group were higher than senior group. The factor scores of somatization (P < 0.05), interpersonal sensitivity (P < 0.05) and anxiety (P < 0.05) in long course group were higher than short course group. The factor score of interpersonal sensitivity (P < 0.05) in low educated group was higher than highly educated group. CONCLUSION Psychiatric disorders are prevalent in pharyngitis patients, especially upon the factors of somatization, obsession, interpersonal sensitivity, anxiety and other (sleep, diet). Male, junior, long course patients and low educated are in high risk group.
Anxiety ; complications ; Asian Continental Ancestry Group ; Factor Analysis, Statistical ; Female ; Humans ; Male ; Mental Disorders ; complications ; Personality Inventory ; Pharyngitis ; complications ; psychology ; Prevalence

Objective To acknowledge the spouse comorbid conditions of diabetes patients by comparing the average blood sugar level of the spouse of the diabetes patients with normal Guangzhou residents.Methods To sift and analyze the spouse test results of 152 hospitalized diabetes patients from January 2012 to January 2013,by testing the blood sugar level of the experimental subjects,comparing the different blood sugar level results between when they were with empty stomach and 2 hours after oral glucose tolerance test (OGTT).Results There were 32 cases with diabetes,9 cases with impaired glucose tolerance (IGT) and 11 cases with impaired fasting glucose (IFG) in the above 152 experimental subjects.The morbidity rates were 21.05％,5.92％ and 7.24％.All the above results were higher than the Guangzhou residents survey level,there were statistical differences between them.Conclusions The high morbidity rates of diabetes and impaired glucose regulation in diabetes patients spouse are obvious.The works for diabetes sifting and analyzing together with the health education instruction need to be strengthened.

Objective To get genetic information of VP 1 coding region of HEV 71 in Beijing in 2008.Methods Enteroviruses were isolated from samples of throat swabs collected from 33 HFMD patients within 3 days after onset by rhabdomyosarcoma(RD) cells and identified by RT-PCR method with specific primers to human enteroviruses,then VP 1 coding region was amplified and sequenced by Sanger dideoxy.Bioedit 7.0.5 and MEGA3.1 were used for the nucleotide sequence alignment and phylogenetic analysis.Results 16 virus strains were isolated from 33 samples,of which 14 strains were identified as HEV 71 and 2 were CVA 16,and 1 was co-infected with CVA 16 and HEV 71.Sequence analysis of VP 1 nucleotide sequences of 10 HEV 71 isolates showed that homologous analysis of nucleotide identity amino acid 95.5%～100% and 98.9%～100% respectively;representative strains of Fuyang in 2008,nucleotide identity was 95.4%～99.1%;with representative strain of C 4 nucleotide identity was over 92%.Phylogenetic analysis of HEV 71 strains for the nucleotide sequence of VP 1 coding region clarified that the HEV 71 isolates in Beijing belonged to C 4a cluster of C 4 sub-genotype and 10 strains formed four relatively separated clusters.Conclusions The HEV 71 viruses isolated from children of HFMD in Beijing belonged to C 4 sub-genotype,and C4a cluster which was the predominant in China since 2004.According to phylogenetic analysis,HEV 71 which belonged to more than 4 different clusters were circulating in Beijing in 2008.More virological suggestion for disease control and prevention,and information of HEV 71 molecular epidemiology need to be collected urgently due to the successive large epidemic of HEV 71 in China.

OBJECTIVE:To systematically review the clinical efficacy and safety of sirolimus(SRL)versus tacrolimus(Tac) for anti-rejection after renal transplantation,and provide evidence-based reference for clinical treatment. METHODS:Retrieved from PubMed,EMBase,Medline,Science Direct,Cochrane library,CJFD,VIP and Wanfang Database,randomized controlled tri-als(RCT)about SRL(test group)versus Tac(control group)for anti-rejection after renal transplantation were collected. Meta-anal-ysis was performed by using RevMan 5.2 software after data extraction and quality evaluation. RESULTS:Totally 5 RCTs were in-cluded,involving 594 patients. Results of Meta-analysis showed,there was no significant difference in the incidence of acute rejec-tions [RR=1.26,95%CI(0.82,1.93),P=0.30],graft loss rate [RR=0.91,95%CI(0.32,2.55),P=0.85] and mortality rate [RR=0.87,95%CI(0.34,2.22),P=0.77] in 2 groups;while the infection rate in test group was significantly lower than control group, the difference was statistically significant [RR=0.13,95%CI(0.04,0.40),P<0.001]. CONCLUSIONS:Compared with Tac,SRL has the same anti-rejection effect after renal transplantation,not changed graft loss rate and mortality rate,but reduced the infection rate with higher safety.

Objective To analyze the clinical distribution and drug resistance of clinically isolated strains of Acinetobacter Bau-mannii(ABA)in our hospital from January 2007 to December 2012 so as to provide the basis for the rational use of antibacterial drugs in clinic.Methods The API microbiological culture and identification system was adopted to conduct the ABA culture,identi-fication and drug susceptibility analysis on the clinical specimens submitted by various clinical department and the drug resistance of the strains was analyzed by using WHONET5.6 software.Results 791 strains of ABA were isolated in the recent 6 years,and the isolating rate of ABA in the pathogenic bacteria was 7.8%;The ABA detection was dominated by the sputum (80.4%)and the throat swab(10.9%)specimens;the distribution of isolated ABA strains were relatively widespread;the infection rates of the pa-tients were higher in ICU (18.8%),cadre wards(17.6%),respiratory medicine department(13.3%)and neurosurgery department (12.3%).During these 6 years,the drug resistance rate of less than 50% included imipenem(IPM)and cefoperazone sodium/sul-bactam,and the resistance rate in most of commonly used antibacterial drugs showed an increasing trend,especially IPM.Conclusion The drug resistance of ABA is more serious and the resistance rate of clinically isolated strains to most of antibacterial drugs in our hospital during 2007- 2012 showed an increasing trend.Dynamically monitoring the ABA distribution and the drug resistance changes conduces to guide the rational use of antibacterial drugs in clinic,prevent the nosocomial infection and reduce the generation of pan-drug resistant strains.

OBJECTIVE: To investigate the characteristics and regularity of adverse drug reactions (ADR) induced by fluoroquinolones. METHODS: 308 fluoroquinolones-induced ADR cases in 231 literatures were collected from domestic medical journals recorded in CHKD from Jan.1994 to Jun.2009 and analyzed statistically. RESULTS: There was no marked relation between the ADR induced by fluoroquinolones and the gender or age of the patients. 76.62% of ADR cases were mainly caused via intravenous drop infusion. 33.44% of ADR occurred within 2～5 days after medication, leading the first place in term of ADR incidence was levofloxacin (19.81%). ADR mainly appeared as injury of central nervous system (30.43%), followed by lesion of skin and appendants (20.58%). 6 severe ADR induced shock and death. CONCLUSION: Great importance should be attached to monitoring of ADR induced by fluoroquinolones and rational use of drug to guarantee safety and effectiveness of medication.

Objective To explore the clinical application of fiberoptic bronchoscopy with bronchoalveolar lavage(BAL) in patients with mechanical ventilation.Methods Sixty patients with mechanical ventilation were randomly divided into control group ( n =30) and treatment group ( n =30).All patients accepted anti-infection,nutritional support treatments.The patients in the control group accepted routine treatment;others in the treatment group were treated with BAL.The therapeutic effects in the two groups were evaluated and compared.Results The treatment effects in treatment group was better than that in control group( P ＜0.05).The improvement of blood gas analysis in treatment group was better than in control group (P ＜ 0.05 ),the time of mechanical ventilation,length of stay in treatment group were shorter than those in the control group ( P ＜ 0.05 ).The number of success to remove ventilator,organs failure and hospital mortality in treatment group were less than those in the control group ( P ＜ 0.05 ).Conclusion Fiberoptic bronchoscopy with BAL treatment could be safe and effective for patients with mechanical ventilation.It deserves to be popularized.