Objective To study the expression of RUNX3 mRNA in primary liver cancer (PHC) tissue and its surrounding normal tissue,and its clinical significance.MethodsReverse transcription polymerase chain reaction (RT-PCR) was performed to detect the expression of RUNX3 mRNA in tumor and peritumor tissues in 51 patients with PHC.The relationship between RUNX3 mRNA expression and some clinical pathological parameters was analyzed.ResultsThe relative expression values of RUNX3 mRNA in the tumor tissue and the surrounding normal tissue were 0.4509±0.0963 and 0.9147± 0.0222,respectively.The difference was significant (t=33.6087,P＜0.001).The RUNX3 mRNA expression in tumor tissue correlated with some clinical pathological parameters including low tumor differentiation,positive cancer embolus and intrahepatic invasion and metastasis.The RUNX3 mRNA expression was not correlated with other clinicopathological parameters including gender,cancer diameter,cancer location,hemorrhage and necrosis of cancer,and histotype.ConclusionRUNX3 may be a new tumor suppressor gene for PHC.

Objective To study the similarities and differences of information needs between cancer patients treated with radiotherapy and chemotherapy, analyze the causes for cancer patients' different choices, and provide reasonable and disease-related information for cancer patients. Methods A total of 102 and 119 cancer patients treated with radiotherapy and chemotherapy relatively were asked to complete the questionnaire (IPQCP+2) and two questions about the total information capacity and content requirements. Results Two groups had different needs of therapeutic information and treatment in IPQCP. Cancer patients treated with chemotherapy needed more widely-ranged information than patients underwent radio-therapy. Conclusions Most of cancer patients treated with radiotherapy and chemotherapy need to be informed of disease-related information, among which the content of therapeutic information and total information is relative to the treatment. Nurses should provide disease-related medical information based on their different needs, as to improve the life quality of cancer patients.

BACKGROUND:Hydrophobic acrylic foldable intraocular lens should be evaluated biologicaly with New Zealand rabbits as implant objects prior to clinical trial. OBJECTIVE:To observe the biological safety of hydrophobic acrylic foldable intraocular lens. METHODS: Twelve New Zealand rabbits were enroled. The right eyes were implanted with self-developed hydrophobic acrylic foldable intraocular lens (Shenyang Baiao Medical Device Co., Ltd., China) as experimental group, and the left eyes were implanted with Acrysof IQ SN60WF (Alcon, USA) as control group. RESULTS AND CONCLUSION: After implantation, there were no significant differences in the cornea, anterior chamber, implant position, posterior segment of eyebal between two groups. Tissue proliferation had no obviously difference between the two groups, and there were cornea and lens pouch inflammations. No macrophages and other inflammatory cels were visualized on the surface of intraocular lens, and fibrous tissues were found on the intraocular lens surface and in the haptics root. These findings suggest that the self-developed hydrophobic acrylic foldable intraocular lens has no difference from Acrysof IQ SN60WF widely used in clinic, and it has the biological safety.

With the advent of the aging society, there will be a wide range of applications if novel intelligent multifunctional nursing beds can be developed for hospitals, bead houses and families at the same time. By listing and analyzing existing products, this paper summarized four function categories for multifunctional nursing beds, including security assurance, treatment aid, comfortability optimization, and human-machine interaction and communication. Finally, by comparing existing functions and potential user requirements, this paper proposed four function development trends, including physiological parameter monitoring, sleep aid, intelligent temperature control, and video communication.
Beds ; Equipment Design ; Monitoring, Physiologic ; instrumentation ; Nursing Care

BACKGROUND: The risks in pregnancy to the developing fetus result in cerebral palsy after delivery. Therefore, the study on etiology of cerebral palsy should transfer to biological field of embryonic development and stress on multi-factor analysis on relevant environment of pregnant mother,hereditary factors and relevant diseases.OBJECTIVE: To analyze the main risks of incidence of infantile cerebral palsy.DESIGN: Pair design at ratio of 1:2 and Logistic regression analysis of multi-factors were applied.SETTING: Prevention Medical Research Room of Jiamusi University and Department of Epidemiology, College of Public Health, Harbin Medical University.PARTICIPANTS: Totally 103 cases of infantile cerebral palsy were collected from Jiamusi Treatment and Education Center of Cerebral palsy,Shanyang Pediatrics Hospital and Qingdao Pediatrics Hospital from December 2002 to September 2003, of which, 68 cases were male and 35 cases were female. The guardians were in the know and agreed with the investigation. The pair principle at ratio of 1:2 was adopted, based on which, 206 cases of sick children with non-cerebral palsy and disorders in neurological system and healthy children treated at same period in the hospitals were collected as the control. The match conditions in the control were same sex, same nationality and difference in age ± 3 months.sy and healthy children was self-designed. The investigated items included mainly: Factors before delivery (hereditary factors): family history of similar disease; mother's factor: general situations, such as occupation, educational background and age of delivery; behavioral factors of birth: including times of pregnancy, times of delivery and abnormal delivery; healthcare and nutrient in pregnancy: such as examination before delivery and intakes of various foods; sickness or abnormal manifestations in pregnancy: including pathogen infection, pregnant syndromes and vaginal bleeding; medication in pregnancy, fetal placents factors: such as neck winding, mass weight of birth, twin pregnancy, factors during delivery (complications in delivery,methods of delivery) and neonatal factors (various kinds of disorders in neonates). Questionnaire of investigation was performed by looking-up medanalysis was done on single factor and multi-factors. The factors that presented statistical significance in single-factor analysis, significant difference (P ＜ 0.05) and P value near to 0.05 were adopted to establish the model of multiple Logistic regression of main risks of cerebral palsy and conditional Logistic regression analysis was carried on.MAIN OUTCOME MEASURES: Analysis on the effects of factors before delivery, during delivery and in neonatal phase on incidence of cerebral palsy.RESULTS: Totally 103 cases of cerebral palsy and 206 cases of healthy factors: Occupation of mother was associated with cerebral palsy. Worker and age ≥34 years were the risks of cerebral palsy (OR=13.333, 2.864,eating fish and sea products in pregnancy, pre-delivery body temperature,mass weight of birth ＞ 4 000 g and pregnant weeks of 37 to 42 were the protective factors of cerebral palsy (OR=0.028-0.576, P ＜ 0.05-0.01); abnormal delivery history and vaginal bleeding in pregnancy were the risks of ery: Apgar score and caesarian section were the protective factors of cerebral palsy (OR=0.458, 0.343, P ＜ 0.01); forceps delivery and delivery with oxytocin were the risks ofcerebral palsy (OR=16.338, 2.116, P ＜ 0.01,of newborn and neonatal intracranial hemorrhage were the risks of cerebral sion analysis: Forceps delivery was the highest risk (OR =70.668) and the sequence risk degree from high to low was asphyxia of newborn, elder age of pregnant mother, vaginal bleeding in pregnancy and abnormal mass weight of birth (OR=19.083-4.138, P ＜ 0.05-0.01). Eating fish and sea products in pregnancy and caesarian section were the protective factors (OR=0.324; 0.262, P ＜ 0.01, 0.05).CONCLUSION: Risks of cerebral palsy are mainly focused on pregnant period and perinatal period. It is suggested to prevent and eliminate actively various risks of cerebral palsy before, during and after delivery (such as forceps delivery, asphyxia of newborn, elder age of pregnant mother,vaginal bleeding in pregnancy and abnormal mass weight of birth) so as to reduce the incidence rate of cerebral palsy.

Objective To explore the feasibility of DSCT dual-energy technique in pulmonary mass lesions.Methods A total of 100 patients with pulmonary masses underwent conventional plain CT scan and dual-energy enhanced CT scan.The virtual non-contrast (VNC) images were obtained at post-processing workstation.The mean CT value,enhancement value,signal to noise ratio (SNR),image quality and radiation dose of pulmonary masses were compared between the two scan techniques using F or t test and the detectability of lesions was compared using Wilcoxon test. Results There was no statistically significant difference among VNC ( A ) ( 32.89 ± 12.58 ) HU,VNC (S) ( 30.86 ± 9.60) HU and conventional plain images (35.89 ± 9.99 ) HU in mean CT value of mass ( F =2.08,P ＞ 0.05 ).There was statistically significant difference among VNC ( A ) ( 3.29 ± 1.45 ),VNC (S) ( 3.93 ± 1.49 ) and conventional plain image (4.61 ± 1.50) in SNR ( F =6.01,P ＜ 0.05 ),which of conventional plain scan was higher than that of VNC.The enhancement value of mass in conventional enhanced scan(60.74 ± 13.9)HU and distribution of iodine from VNC (A) ( 58.26 ± 31.99 ) HU was no statistically significant difference ( t =0.48,P ＞ 0.05 ),but there was a significant difference between conventional enhanced scan (56.51 ± 17.94 ) HU and distribution of iodine from VNC (S) (52.65 ± 16.78 ) HU (t =4.45,P ＜ 0.05 ).There was no statistically significant difference among conventional plain scan ( 4.69 ± 0.06 ) and VN C ( A ) ( 4.60 ± 0.09 ),VNC (S)(4.61 ±0.11 ) in image quality at mediastinal window ( F =3.014,P ＞ 0.05 ).The appearance,size,internal features of mass (such as necrosis,calcification and cavity) were showed the same in conventional plain scan,VNC (A) and VNC (S).Of 41 patients with hilar mass,18 patients were found to have lobular and segmental perfusion decrease or defect. Perfusion defect area was found in 59 patients with peripheral lung mass. The radiation dose of dual-energy enhanced scan was lower than that of conventional scan.Conclusion The virtual non-contrast,distribution of iodine and pulmonary virtual perfusion images can be obtained by DSCT dual-energy technique in one scan,which has a potential clinical value in the thorax.

Objective To discuss the effects of applying electric heating pattern instrument in experimental teaching of fixed partial denture technology.Methods Totally 98 prosthodontic undergraduates of 2008 and 2009 grades were selected in this study； 46 students of 2008 grade were taken as control group and 52 students of 2009 grade were taken as experimental group.Students in experimental group made wax patterns with electric heating pattern instruments to melt inlay wax while those in control group made wax patterns with instruments heated by alcohol lamps.Teaching effects were evaluated by experimental test scores and questionnaires.Results Test scores of experimental group were (22.6± 1.8),obviously higher than those of (22.6-± 1.8) in control group (P ＜ 0.05).Satisfaction degree of experimental group were increased significantly compared with that of control group based on the resuits of questionnaire.Conclusions Applying electric heating pattern instrument in making wax patterns in experimental teaching of fixed partial denture technology is easy to operate and can improve the quality of wax pattern and working efficiency as well as enhance students' confidence,therefore it is worthy further spreading.

Objective We have revised the Sunnybrook facial nerve grading system which has been widely recog?nized in the world to a Chinese version and verified its credibility and validity. Methods First we translated and edited the Sunnybrook facial nerve grading system using Chinese.Nine doctors, who were selected from different hospitals, then scored twice on the same video of 90 patients with facial nerve palsy using the Chinese version of the Sunnybrook facial nerve grading system and grading standard separately at one month interval. The consistency and the repeatability of the Chinese version of the Sunnybrook facial nerve grading system were evaluated. Results The Cronbach 's alpha value was 0.92 and the average coefficient of repeatability (CR) was 12.72, indicating that the Chinese version of the Sunny?brook facial nerve grading system had a high degree of internal consistency and repeatability. The ICC values on the as?sessment and evaluation for the internal measurement were 0.97 and 0.98, respectively. The scored results were compara?ble between doctors who were not familiar with this disease and those who were very familiar with the disease. The aver?age ICC was 0.97(95%CI:0.95~0.99). Conclusions The Sunnybrook facial nerve grading system has good reliability and consistency and hereby recommend Chinese doctors to use it.

Objective To use evidence-based nursing on patients with artificial airway to the practice of stomach tube, and to evaluate the self made guidance for difficult gastric tube placement in patients with artificial airway.Methods Forty patients with artificial airway and were difficult to put the tube, and admitted to Department of Critical Care Medicine of Harrison International Peace Hospital Affiliated to Hebei Medical University from April to December in 2016 were enrolled as observation group. Through the evidence-based nursing strategy, the related literatures at home and abroad were collected to search clinical evidence and formulate and implement the nursing program, the gastric tube was inserted into the stomach tube under the direct vision of the laryngoscope. Thirty-six patients of difficult gastric tube placement with artificial airway straightly under the direct vision of the laryngoscope from August 2015 to March 2016 were retrospectively analyzed as the control group. The success rate of first catheterization, indwelling time, throat edema and bleeding of pharyngeal mucosa were compared between the two groups.Results All patients were enrolled in the final analysis. In the control group, 28 patients were successfully placed once, while 8 failed. Only 1 patient in observation group failed to catheterize, and successful placed after symptomatic treatment, the one-time success rate of catheter was significantly higher than that of control group (97.5% vs. 77.8%), and catheter time was significantly shortened (minutes: 4.8±1.2 vs. 5.1±1.0), the difference was statistically significant (bothP < 0.05). There were 2 patients with laryngeal edema in the control group and 4 patients of pharyngeal mucosal hemorrhage. In the observation group, there was no laryngealedema and laryngeal edema occurred in the patients with laryngoscopy, and only 1 patient had a hemorrhage of pharyngeal mucosa.Conclusion Using self made guiding device can effectively insert the difficult gastric tube in patients with artificial airway, and increase the one-time success rate of intubation, shorten the catheter time, and have a trend in reduce complication as compared with traditional gastric tube placement.

Objective To explore the quantity and ultrastructure of dopaminum (DA) neuron in nigra-striatum, pathological basis of the successful animal model of cerebral palsy (CP) by hyperbilirubinemia, to provide the evidence for clinical therapy and rehabilitation. Methods Thirty homozygous newborn rabbits (2~5 d) were randomly divided into 2 groups: a control group(n=10) and a model group(n=20). The rabbits in the control group were administrated bilirubin intraperitoneally, with a total dose of 300 mg/kg, while those in the model group were administrated with saline at the same dose. All the animals were fed well for 45 days until they were sacrificed for observation of the ultrastructure of brain tissue and quantification of DA neurons. Results The ultrastructure of nigra-striatum of the control group demonstrated significant changes; The quantity of nigra-striatum DA neurons of the control group decreased. Conclusion The ultrastructure of nigra-striatum of the control group has had obviously changes. The nigra-striatum DA neuron of CP rabbits was impaired and the quantity of nigra-striatum DA neuron of CP rabbits was reduced.