Overexpression of epidermal growth factor receptor (EGFR) in esophageal cancer has been related to tumor grade, vascular invasion and metastasis. The level of EGFR expression may be a predictive marker of esophageal caneer's sensitivity to chemoradiotherapy. EGFR inhibitors have been indicated as an effective targeted therapy, and promising results have been observed in phase Ⅱ and Ⅲ clinical trials.

Tumor cell proliferation activity is related with tumor biological behavior. PCNA (proliferating cell unclear antigen, PCNA) is a kind of histone nucleoprotein, is associated with cell proliferation and DNA synthesis,can reflect the degree of cell proliferation and cycle period, it is as a cell proliferation activity indicator, has been widely used in a variety of tumor research,the expression of PCNA in common head and neck tumor can be used as the biological behaviour and prognosis judgement.

Objective To analyse the mutation of pathogenic gene TSC2 in tuberous sclerosis complex (TSC). Methods Using polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP), all the 41 exons of TSC2 gene were analyzed in 4 TSC cases(include 1 suspect case) from one family and 1 sporadic TSC case ,and compared with the kin familial controls and kinless normal controls. Results Missense mutation on exon33 1346S→P (4037T→C) of TSC2 was found in 4 familial cases, and no mutation of TSC2 gene was found in the sporadic case and all the health controls. Conclusion Missense mutation on exon33 (1346S→P,4037T→C)is a new discovery in TSC2 gene of patients with TSC.

OBJECTIVE: To evaluate the safety and efficacy of Wuling Capsule combined with Deanxit in treating post-stroke depression (PSD). METHODS: One hundred and fourteen patients with PSD were randomly divided into Wuling Capsule-treated group (n=39), Deanxit-treated group (n=37) and Wuling Capsule plus Deanxit-treated group (n=38). Patients in Wuling Capsule-treated group were administered with three Wuling Capsules three times a day, and patients in Deanxit-treated group were administered with Deanxit 10.5 mg twice daily, while patients in the Wuling Capsule plus Deanxit-treated group were administered with both Wuling Capsule and Deanxit. Patients in the three groups were all treated for six weeks. Treatment efficacy was evaluated with Hamilton Depression Scale (HAMD) and the side effects were evaluated with Treatment Emergent Symptom Scale (TESS) before treatment and after 2-, 4-, and 6-week treatment. The blood and urine routine examinations were performed, and the hepatorenal functions and electrocardiogram were examined as well. RESULTS: There was no statistical difference in the total efficacy rate between Wuling Capsule-treated group and Deanxit-treated group (64.1% vs 64.9%, P>0.05), but the total efficacy rate of Wuling Capsule plus Deanxit-treated group was higher than that of the monotherapy (89.5% vs 64.1%, 89.5% vs 64.9%, P<0.05). There were no significant side effects in Wuling Capsule-treated group, while the incidence of side effects was 9% in both groups administered with Deanxit. CONCLUSION: The efficacy of Wuling Capsule plus Deanxit is better than that of the monotherapy in treating PSD.

0.05, respectively), but those to noradrenaline (NA: 0.01?mol/L), histamine (His: 3?mol/L), and 5-hydroxytryptamine (5-HT: 0.1?mol/L), significantly reduced (P0.05, respectively). Under atropine or Ani pretreatment, the NA-, His- and 5-HT-elicited contractions in endothelium-denuded aorta were similar to those in endothelium-intact aorta. Conclusion Atropine and Ani can inhibit receptors-mediated constrictions of rabbit aortic vascular smooth muscle cells; the actions are endothelia independent.

Objective To study the protective action of Semen Ziziphi Spiosaeand on acute myocardial ischemia and its effecte on infarcted area. Methods Left anterior descending(LAD) of coronary artery was ligated to cause the big rat's acute myocardial ischemia, and electrocardiogram (ECG) was used to appraise the effect of sour jujube kernel total soap on the myocardial infarcted area. Results Compared with control group, the experimental group treated with sour jujube kernel total soap glucosidal showed significantly smaller infarcted area; and ECG showed the heart rhythm, S-T piece and T wave value were significantly decreased in the experimental group. Conclusion Sour jujube kernel total soap glucosidal can reduce the myocardial infarcted area caused by ligature of LAD, so as to protect the cardiac muscle.

Objective To assess the disc injury in neurologically intact thoracolumbar bust fractures and to evaluate the correlation between the severity of disc injury and the parameters of vertebral body damage as as to formulate radiologic index for further MRI examination.Methods A retrospective case series review was made on 22 patients (44 discs) with thoracolumbar burst fractures without neurologic deficit treated from January 2015 to December 2015.Vertebral level involved was from T11-L2.There were 12 males and 10 females,with the mean age of 47.9 years (range,43-60 years).MRI were used to evaluate the severity of disc injury.Among the 44 discs,18 were Grade 0,11 were Grade 1,12 were Grade 2,and three were Grade 3.Then patients with Grade 0 and 1 were taken as disc intact group and other 12 patients disc rupture group.Vertebral wedge angle,local kyphosis angle,anterior and posterior vertebral body height and canal compromise were measured on CT to determine the damage of fracture.Spearman correlation coefficient was used to analyze the correlation between the severity of disc injury and the parameters of vertebral body damage,and receiver operative curve (ROC) was used to assess the effectiveness of these indexes in predicting disc injury.Results Vertebral wedge angle and anterior/posterior vertebral body height ratio were correlated with the severity of superadjacent disc injury (P ＜ 0.05 or 0.01).Canal compromise,vertebral wedge angle and anterior/posterior vertebral body height ratio were significantly different between the two groups (P ＜ 0.05 or 0.01).ROC results showed the area under curve of canal compromise,vertebral wedge angle and anterior/posterior vertebral body height ratio were 0.758,0.762,and 0.867 respectively,indicating no significant difference (P ＞ 0.05).Threshold of canal compromise,vertebral wedge angle and anterior/posterior vertebral body height ratio were 21％,15.4°,and 0.69 respectively.Conclusions Vertebral wedge angle and anterior/posterior vertebral body hight ratio are correlated with the severity of superadjacent disc injury.Canal compromise ＞21％,VWA ＞ 15°and anterior/posterior vertebral body height ratio ＞ 0.69 may be the practice preferences of indications for ordering a MRI in patients with neurologically intact thoracolumbar bust fractures.

Objective:To analyze the characteristics of regional lymph node metastasis in patients with resectable non-small cell lung cancer (NSCLC) and assess its clinical significance in surgical mediastinal lymph dissection and the target volume definition of postoperative radiotherapy. Methods:We retrospectively reviewed 810 patients with NSCLC, and analyzed the metastatic frequency of each regional lymph node station as well as the correlation between tumor location and regional lymph node metastases. Results:Re-gional lymph node metastases were significantly associated with the age of patients, histology, tumor size, and tumor location (P=0.013, 0.000, 0.009 and 0.000, respectively). Conclusion:The younger patients with left lung adenocarcinomas and large tumor size tended to regional lymph node metastases. The trend of regional lymphatic drainage in the lobes of lung occurred differently. The prior location of involved regional lymph nodes in different lobes of the NSCLC patients was as follows:The station 2-4 for right upper lobe tumors, the station 2-4 and 7 for right middle lobe tumors and right lower lobe tumors, the station 5-6 for left upper lobe tumors, and the station 5-6 and 7 for left lower lobe tumors. We should pay more attention to the regions regarding the higher frequencies of lymph node metastases, when determining the extent of lymph node dissection or delineating the target volume of postoperative radiotherapy for NSCLC patients.

Objective To investigate cause analysis and treatment strategy of cage migration after lumbar interbody fusion.Methods Retrospective study was performed on 9 cases with cage migration after lumbar interbody fusion from January 2009 to February 2015 in our hospital.There were 4 males and 5 females,and mean age was 61.6 years (rang,38-75 years).The types of cage included Titanium metal cage used in 3 cases,cylindrical thread cage in 1 case and PEEK cage in the other cases.Bilateral instrumented posterior lumbar interbody fusion was found in 7 cases,and unilateral fixation in 2 cases.Analyze the risk factors of cage migration and the strategies of revision surgery,and evaluate the radiological outcomes and clinical efficacy of revision surgery.Results Risk factors of cage backward migration are as follows:nucleus pulposus left too much in 6 cases,poor cartilage endplate resection in 4 cases,small size of cage selection in 5 cases,unsatisfied cage placement in 2 cases,and improper operation in 1 case.Follow-up survey was fulfilled in all patients,the follow-up time was 6 to 32 months,and bony union was detected in all patients.No cage re-migration,non-fusion,or loosen pedicle screw was found during follow-up period.Clinical symptoms were all improved after revision.Conclusion The causes of cage migration after bilateral or unilateral instrumented transforaminal lumbar interbody fusion were complicated.Risk factors of cage migration may be poor intervertebral space preparation,small cage size,and improper cage placement,which may be not associated with unilateral fixation.Excellent or good radiological outcomes and clinical efficacy depend on a reasonable revision surgery.

Objective To investigate the treatment and prognosis of patients with papillary thyroid microcarcinoma. Methods The clinical and following-up data of 124 patients with papillary thyroid microcarcinoma treated at the Department of Head and Neck Surgery of Cancer Centre, SUN Yat-sen University from Jan 1990 to Dec 1999 were analyzed retrospectively for mortality and survival rate ( KaplanMeier). A multivariate analysis was performed in these patients by Cox proportional hazard model. Results The overall 10-year and 15-year survival rate of all 124 patients with papillary thyroid microcarcinoma were 94.9% and 92.5% respectively. The univariate analysis showed the prognostic factors significantly influencing the survival of patients included age (being worse for those of 35 years and older) at presentation, and the status of distant metastasis ( all P ＜ 0. 05 ) , while gender, incidentally found at surgery, the size of primary tumor, the status of neck lymph node metastasis, recurrence after a primary resection, and the extent of surgical resection did not significantly effect the prognosis. Multivariate analysis showed the age more than 35 years at presentation was an independent risk factor indicating worse prognosis (P = 0. 045 ). Conclusioas The prognosis of patients with papillary thyroid microcarcinoma is satisfactory, but that is poor for those patients 35 years old and up at presentation and with distant metastasis.