Objective To study the clinical characteristics and potential influencing factors of rehabilitation of cognition in post-stroke patients with aphasia.methods Forty-one patients with aphasia after ischemic stroke,admitted to Nanfang hospital and Nanfang Yanling Hospital from June 2015 to November 2016 were included.Their primary clinical information and NIHSS scores were registered.In acute phase,Aphasia Battery of Chinese and Boston Diagnostic Aphasia Examination was used to evaluate language ability and classify the aphasia type.Non-language-based Cognitive assessment (NLCA) was used to assess patients' nonlinguistic cognition.Besides,the assessment of post-stroke depression and dependence degree of daily living activities were performed.3 months later,patients were evaluated again.A total of 30 aphasia patients completed the final follow-up.Result s(1) Compared with the baseline in patients with aphasia after stoke,all domains of language ability were significantly improved after 3 months(P<0.05).(2)Every nonlinguistic cognitive score was significantly increased within 3 months(total NLCA score(65.83±13.02 vs 48.00±25.11),memory(17.23±2.49 vs 13.30±5.42),visual spatial ability(10.67±2.43 vs 8.07±3.75),logical reasoning ability(6.53±1.48 vs 4.97±2.43),attention(25.57±5.79 vs 17.43±12.33),executive function(5.77±3.47 vs 4.20±4.23),all P<0.01).(3)The NLCA total score (62.40±14.23 vs 72.70±6.34)and scores in visual spatial (9.95±2.67 vs 12.10±0.74)and abstract reasoning(6.05±1.54 vs 7.50±0.71) of persisting aphasia patients were significantly lower than that of aphasia recovered group(all P<0.05).(4) Multivariate regression showed that initial NLCA score had a negative predictive effect on cognitive function improvement of aphasia patients 3 months after stroke(β=-0.603,P<0.01).Conclusion The rehabilitation of cognitive function in patients with aphasia after stroke is similar to that of language function.Patients with persisting aphasia may have worse performance on nonlinguistic cognition and ability of daily living,even with more serious depressive emotion.Evaluation of nonlinguistic cognition in post-stroke aphasia patients in acute phase is necessary and the score seems to be an important predicting factor of 3-month cognition.

Objective To investigate efficiency of centipede extracts on apoptosis induction, proliferation inhibition to Human A549 cell line and growth suppression of subcutaneous transplanted sarcoma in nude mice. Methods Centipede extracts prepared by enzymolysis and acetone precipitation methods were used to treat human lung cancer A549 cell line. Proliferation inhibition was evaluated by MTT assay and half inhibit concentration (IC50) was calculated. Cell morphological change and apoptosis were detected by flow cytometry and Hoechst stain. The subcutaneous transplanted sarcoma models were prepared with nude mice and randomly divided into model group, control group and centipede extracts group, with 10 mice in each group. Changes of tumor volume, quality and anti-tumor rate were observed.Results In vitro experiment, proliferation of A549 cells was inhibited with dose-dependency and IC50 value was 0.603 mg/mL. The G0/G1 phase of cells was down regulated and G2/M and S phase cells were up-regulated. The apoptotic character cells were been found by Hoechst stain. In vivo experiment, the tumor weight and volume decreased significantly compared with model control group, with statistical significance (P<0.01).Conclusion The centipede extracts shows dose-dependent anti-proliferative effect on A549 cells, which can induce apoptosis by arresting A549 cells at G2/M phase and suppressing growth of subcutaneous transplanted sarcoma of lung cancer in nude mice.