Objective To evaluate the efficiency of entecavir in chronic hepatitis B (CHB)patients.Meathods 60 cases of pa-tients treated with entecavir 0.5 mg/d.Observed the negative rate of HBV DNA and ALT after 3,6 and 12 months.Used immuno chemiluminescence to observe the HBeAg titer after medication.Results The negative rate of ALT and HBeAg, HBeAg conversion rate were 91.7%,86.7% and 16.7%,respectively.The titer of HBeAg was low at baseline,the patients would had higher HBeAg seroconversion,there were significant differences with other groups.The titer of HBeAg decreased≥90% patients was significantly higher in patients with < 90% on the 3 months.Conclusion The HBeAg titer could be predicted ETV for treatment of HBeAg positive CHB patients.

Sepis s is the leading cause of death in critically ill children,but it lack biomarkers with a higher sesn itivity and specificity for sepsis diagnosis,severity assessment,and prognostication.Soluble uroki-nase plasminogen activator receptor( suPAR) ,as a nvo el receptor ts imual ted by inflammation,is involved in the plasminogen activating pathways,inflammation and migration,adhesino ,chemotaxis,signal transduction of v ra ious immuen cell.suPAR serum levels have been shown to significantly incer ase in various infectious dis-easse .This revei w provided a ed scriptive overview of the role of suPARd etection in sepsis judgmne t and prognostication,hoping to find a perfetc biomarker for timely evaluait ng of chidlr en with spe sis and screening for childrena dmitted to the intensive care uin t in order to improve outcome and reduce mortality.

Objective: Pemetrexed (PEM) is a multi-targeted chemotherapeutic agent for antifolate drugs. PEM has become the standard agent for the second-line treatment of advanced non-small cell lung cancer (NSCLC). This study aims to review and analyze the clinical efficacy and adverse reactions of PEM combined with carboplatin with respect to NSCLC treatment. Methods: A total of 40 patients suffering from NSCLC were selected and confirmed by pathology. On the first day of treatment, the conventional 500 mg/m2 dose of pemetrexed disodium was infused intravenously. On the second day, a combined therapy with carboplatin was conducted based on the conventional dose for a 21-day cycle with at least two cycles for each patient. The therapeutic efficacy and adverse reactions were evaluated and were compared with the proposed regimen of gemcitabine (GEM) combined with carboplatin. Results: After two cycles of the treatment, the curative effects of the PEM and GEM groups were 50% and 45%, respectively. The main adverse reactions are bone marrow suppression and gastrointestinal reactions. The incidence rates of bone marrow suppression, gastrointestinal reactions, amisulpride/AST, urea nitrogen, rash, and hair loss were obviously lower in the PEM group than in the GEM group. Statistically significant differences in adverse reaction were found between the two groups (P<0.05). Conclusion: The use of the combination regimen of PEM with carboplatin showed significantly more clinical effects and less adverse reactions for NSCLC treatment.

Objective To investigate the influence of rosuvastatin on inflammatory factors and immune func-tion in chronic obstructive pulmonary disease(COPD)with pulmonary heart disease.Methods 80 COPD patients with pulmonary heart disease were randomly divided into two groups,40 cases in each group.The control group was treated with conventional medicine comprehensive treatment,the observation group was given rosuvastatin based on the control group.The inflammatory cytokines (TNF -α,IL -1 and hs -CRP)changes,immunoglobulin IgM,IgG and IgA changes situation,and heart,lung function changes related indicators were compared between the two groups before and after the treatment.Results After treatment,the TNF -α,IL -1 and hs CRP levels of the observation group were significantly lower than those before treatment(t =145.5,35.35,57.5,all P <0.05)and those of the control group(t =160.75,11.45,32.99,all P <0.05).After treatment,the IgM,IgG and IgA levels of the observa-tion group were significantly lower than those before treatment (t =4.89 of 18.29,3.42,all P <0.05)and those of the control group (t =2.55 9.67,2.342,all P <0.05 ).After treatment,the FEV1 ,FEV1 /FVC and LVEF of the observation group were significantly higher than those before treatment(t =40.44 33.64,33.79,all P <0.05),while the PAP level was significantly lower than that before treatment (t =24.12,P <0.05),and the FEV1 ,FEV1 /FVC and LVEF of the observation group were significantly higher than those of the control group after treatment(t =16.30, 15.82,18.72,all P <0.05),while the PAP level was significantly lower than that of the control group after treatment (t =13.43,P <0.05).Conclusion Combined rosuvastatin therapy for COPD patients with pulmonary heart disease can effectively reduce body inflammatory response,improve immune function and myocardial contractility,so it can promote functional recovery of heart and lung in patients.

Objective To investigate the relationship among serum levels of suppressors of cytokine signaling-3(SOCS-3),insulin-like growth factor binding protein-1(IGFBP-1) and hyperglycemia in critically ill children. Methods The 64 critically ill children who suffered from sepsis,after surgical repair of congenital heart dis-ease by extracorporeal circulation, and after surgery of severe traumatic from January 2009 to January 2012 in Depart-ment of Pediatric Intensive Care Unit( PICU) of Guangzhou Women and Children′s Medical Center were selected as the research object. According to the blood glucose levels on admission, the 64 children were divided into the normal glu-cose group and the hyperglycemia group. The 15 cases of healthy children in the same period in Guangzhou Women and Children′s Medical Center were selected as the healthy control group. The levels of blood glucose,insulin,SOCS-3,IG-FBP-1 and insulin resistance index( HOMA-IR) were measured and compared among groups when they were on ad-mission. Results (1) The blood glucose of the hyperglycemia group was significantly higher than those in the normal glucose group and the healthy control group[(9. 83±2. 48) mmol/L vs (4. 82±0. 76) mmol/L,(4. 49±0. 81) mmol/L] (P<0. 05). (2) Both of the insulin and HOMA-IR in critically ill children with hyperglycemia were significantly higher than those in the other 2 groups[9. 68(5. 85,17. 35) mU/L vs 4. 08(2. 00,7. 41) mU/L,2. 83(2. 00,10. 10) mU/L;4. 30(2. 70,7. 66) vs 0. 87(0. 39,1. 94),0. 52(0. 38,2. 56)](P<0. 05). (3) The serum SOCS-3,IGFBP-1 levels among the hyperglycemia group,the normal glucose group and the healthy control group were not of significant difference (P>0. 05). (4) The blood glucose was positively correlated with the insulin and HOMA-IR(r=0. 455,0. 773,P<0. 05),but there was no significant correlation among the blood glucose,insulin,HOMA-IR,SOCS-3 and IGFBP-1(P>0. 05). Con-clusions In the critically ill children,hyperglycemia was related to the insulin resistance which can not be evaluated through changes in serum levels of SOCS-3 and IGFBP-1. In addition,it cannot be excluded that the critically ill chil-dren may have insulin resistance and pancreaticβ-cell dysfunction simultaneously.

Objectives To study the influential factors of the von Clauss method and to promote the method for fibrinogen assay in clinical laboratories in China. MethodsKits of foreign and domestic products were used. Calibration curves for fibrinogen determination were established, and calibration plasma was used for comparison. Effects of pH value, thrombin activity, and temperature on the measurement were studied.Results The reproducibility of the von Clauss method using domestic thrombin and related reagents was excellent, the within day CV being 0.039 1, and the between day CV 0.049 7. The percentage variations of calibration plasma determination, with the foreign fibrinogen assay kits using domestic reagents, were +3.17% , +4.76%, and -6.03%, respectively. Determination of a lyophilized plasma using the two sources reagents showed similar results.Conclusions Thrombin and relevant reagents produced in China could be used as a substitute for kits purchased abroad, which would promote the use of von Clauss method in clinical laboratories of China.

Objective To investigate the feasibility and superiority of the lecture based learning (LBL)combined problem-based learning (PBL)for endocrinology teaching practice. Methods Totally 150 clinic medical undergraduates were randomly divided into three groups:LBL group (n=50),PBL group (n=50)and LBL+PBL group (n=50). Questionnaires,written and oral tests were employed to evaluate the teaching effect. ANOVA was used to compare the indicator scores and chi-square test was used to compare grades and passing rate. Results Teaching efficiency,mater of knowledge,capabilities of analysis,teaming and collaboration ability and medical diagnosis thinking cultivation were better in LBL and PBL group than in the other two groups. There were statistical differences in total score(F=4.69, P=0.010)and passing rate(χ2=8.94,P=0.030)among three groups. Conclusions LBL+PBL guarantee the teaching quality and cultivate the comprehensive quality of students; therefore it is superior to LBL and PBL.

This paper recorded experience on clinical treatment of Mumps from the aspects of pathogenesis,syndrome differentiation,and examples of proved cases.The aim of this paper was to summarize and exchange experiences,80 as to facilitate the smdents'interest in study,and to improve teaching level of clinical practice of traditional Chinese medicine.

With the improvement of living conditions,people pay more attention to food sanitation,following which the incidence rate of food allergy is higher and higher.Food allergy threatens public health seriously.It is caused mainly by dominance of T helper type 2-based immune responses,which breaks the dynamic balance of Th1/Th2 immune responses and resultes in over secreting of IL-4,IL-5 and IL-13,production of specific antibody IgE,degranulation of mast cells,alteration of intestinal microflora and so on.At present,a lot of investigations have been reported,of which the probiotics therapy attracted more attention.As the indigenous flora,probiotics could not only prevent and treat food allergy but could also modulate immune responses and recover the dynamic balance of it via secreation of cytokines such as IFN-?,IL-10,TGF-?.

AIM: To investigate the effects of estrogen interference on ab dominal adipose accumulation in C7BL/6J mice. METHODS: Estrogen was administrated to the ovariectomized mice (estrogen-deprived model) and the changes of abdominal adipose weight of the mice in the normal estrous cycle and pro-menopausal were observed. Mice of 3 months old were randomly divided into c ontrol, ovariectomized (OVX), OVX+estrogen 15-days (E d 15 ) and OVX+ estrog en 60-days (E d 60 ) groups. Each group had 10 mice. Mice were raised and sa crificed until 7 months old after ovariectomized. 5 months and 10 months old mic e were divided into control, estrous cycle normal + estrogen 60 days, pro-menop ausal and pro-menopausal+60 days groups. 17?-estradiol time-release pellets ( 0.18 mg/pellet) were given ic in 15 days and 60 days before mice w ere sacrificed. RESULTS: The abdominal adipose weight in OVX gro up increased significantly (P 0.05 ). There was no difference in the abdominal adipo se weight between control and estrous cycle normal + estrogen 60-days groups, a nd between pro-menopausal and pro-menopausal+ estrogen 60-days groups. CONCLUSION: Ovariectomy significantly influences the metabolism of abd ominal adipose that causes adipose accumulation. Estrogen interference, especial ly long-term administration, can attenuate the abdominal adipose accumulation. Exogenous estrogen interference has no effect on abdominal adipose accumulation on cycle normal and pro-menopausal mice.