AIM:To investigate the mechanisms of Myospalax fontanieri bone extracts for macrophages activation. METHODS: Complete Freund's adjuvant was used to induce mouse celiac macrophage activation by ip,then peritoneal exudate cells were cultured in vitro in the present of LPS and IFN-?,concentrations of IL-1?,IL-6,IL-12p40,and TNF-? were by ELISA measurement and the MAC-1 cell proportion was determined by FACS Calibur. RESULTS: Myospalax fontanieri bone had immunosuppressive effect on mouse celiac macrophages,and markedly decreased production of inflammatory Cytokine. CONCLUSION: Inhibition of inflammatory factor may be the mechanism of action for rheumatoid arthritis.

Objective: To investigate the detection ability of two reagents by automated coagulation analyzer ACL-TOP using immune turbidimetric method, and to determine the merits and demerits of them. Methods:Two reagents were compared in the study. We selected 532 cases of hospitalized patients in Anzhen Hospital, 388 cases of which were unstable angina, 60 cases were pulmonary thromboembolism, 70 cases were coronary atherosclerosis, 14 cases were abdominal aneurysm, and the rest were the patients with high blood pressure and coronary heart disease. The plasmas of all the patients were extracted, and D-dimer was detected on ACL-TOP by D-Dimer (referred to as reagent A) and D-DHS (referred to as reagent B). Results:The results of D-dimer increased in different degrees, and there are 88 cases of false positive cases. The case whose FDP and individual D-dimer of D-dimer simultaneous detection was elevating and clinical symptoms were disappeared was detected by the VIDAS instrument platform verification. There were significant differences between A and B. The detection result of reagent A was [1594.15±2286.18], and reagent B was [912.97±1814.06]. There were significant differences between them (t=3.654, P<0.05). In addition, the consistency of the two reagents was poor (r=0.419, P<0.05). Conclusion:Comparing two reagents to detect D-dimer quantitatively by automated coagulation analyzer ACL-TOP, D-DHS owns greater ability to exclude false-positive results, so does the ability of anti-interference with jaundice, hemolysis and chylemia.

BACKGROUND: A large amount of studies have demonstrated that stem cells in the heart of mammal participate in heart self-renewal and endogenous repair.OBJECTIVE: To summarize the source, classification, features of cardiac stem cells and application in heart disease. METHODS: A computer-based online search of PubMed database was performed for articles published between January 2000 and December 2010 related to the source, classification, features of cardiac stem cells and its effects on myocardial regeneration with key words cardiac stem cell. Clinical studies and basic studies were all included. Repetitive studies and Meta analysis were excluded. Finally, 32 articles were included.RESULTS AND CONCLUSION: Cardiac stem cell is a type of stem cells in the heart, with properties of self-renewal and cloning proliferation. It can differentiate into cardiomyocyte and endothelial cell and plays a role in heart injury repair to improve heart function. Cardiac stem cells have been in vitro isolated, cultured and amplified to transplant into animal heart, which lays a foundation for clinical application. However, some questions remain poorly understood as to the homeostasis and dynamic changes, processes involved in their differentiation into heart functioning cells, as well as influential factors and regulation.

The lipoprotein-associated phospholipase A2 (Lp-PLA2), a subtype of the phospholipase A2 superfamily, is produced primarily by macrophages and lymphocytes. Lp-PLA2specifically hydrolyzes oxidized phospholipids on oxidized low-density lipoprotein particles,resulting in production of lysophosphatidylcholine and oxidized fatty acids. Lp-PLA2 is expressed in atherosclerotic plaques and in macrophages within a fibrous cap of unstable plaque.Studies haw shown that the plasma Lp-PLA2 activity increases significantly in patients with ischemic stroke, and Lp-PLA2 may become an independent risk factor for predicting ischemic cerebrovascular events. The selective Lp-PLA2 inhibitor can reduce the inflammatory response,enhance the stability of plaques, and inhibit the formation of atherosclerotic plaques, and may become a new class of drugs for preventing the formation of atherosclerotic plaques.

Objective To observe the changes of endothelin and 6 Keto PGF 1? of patients with pulmonary heart disease and effects of Compound Danshen,to study the role of ET 6 Keto PGF 1? in pulmonary heart disease and pulmonary artery hypertension,and explore a safe and effective drug for pulmonary artery hypertension Methods 40 patients with CPHD and 30 patients with COPD as well as 20 healthy persons as control whose levels of plasma ET and 6 Keto PGF 1? were measured by radioimmunization,meanwhile,PaO 2 was measured by blood gas analyze instrument Results The level of ET in CPHD was higher than that in COPD and healthy group,company with the lower level of 6 Keto PGF 1? After treatment with Compound Danshen,the level of ET was reduced and 6 Keto PGF 1? was elevated Conclusions The balance between ET and PGI is important in modulating pulmonary circulation Compound Danshen can reduce pulmonary artery tension through protecting endothelium,decreasing ET,and increasing PGI 2

Objective: To investigate changes of plasma glucagon level in patients with heart failure (HF) caused by coronary heart disease (CHD) and its clinical significance. Methods: A total of 30 HF patients caused by CHD were selected as HF group, another 30 healthy subjects with corresponding age and gender were regard as normal control group. HF patients received comprehensive therapy of enhancing myocardial contractility, diuretic and vasodilator of 7~10d according to Chinese diagnostic and treatment guideline of chronic heart failure. Changes of glucagon level before and after treatment were observed. Results: Before treatment, plasma level of glucagon in HF group was significantly higher than that of normal control group [(205.67±120.22) ng/L vs. (90.53±20.5) ng/L, P<0.05]. After treatment, plasma level of glucagon [(120.42±30.33) ng/L] significantly decreased than before treatment (P<0.05) in HF group. Conclusion: Plasma glucagon significantly increases in patients with heart failure and gradually decreases to near normal level after treatment. Glucagon level may be regard as one of indexes judging patients’ condition.

Aim To explore the role of prenatal exposure to lipopolysaccharides(LPS)on aortic morphology in the 6-week offspring rats.Methods Twelve pregnant rats were randomly divided into three groups: control group,LPS group,L+P(pyrrolidinedithiocarbamate,PDTC)group.The rats were intraperitoneally administered vehicle,LPS(0.79 mg·kg-1),or LPS plus PDTC(100 mg·kg-1).LPS was given on the 8th,10th and 12th day,where as vehicle and PDTC were given daily from the 8th to the 14th day during gestation.Offspring body weight was measured at 6-week,and histopathological alteration of the thoracic aorta was observed by transmission electron microscopy,the thoracic aorta mRNA and protein expression of connexin(Cx)molecules including Cx37,Cx40,Cx43 and Cx45 in offspring rats were detected by real time PCR,Western blot and confocal laser-scanning microscope.Results Body weight at 6-week offspring rats was significantly higher in LPS group than in control group whatever male or female(P<0.01);and that in L+P group was significantly lower than in LPS group in male rats(P<0.05),but not in female rats.The LPS group thoracic aortas exhibited lesions,including impaired endothelial cells,and the vascular smooth muscle cells were arranged disorder and migration and proliferation;the number of gap junction was increased and longer than control group;the pathological changes were improved to some extent in L+P group.Cx43 mRNA and protein expression in LPS group was obviously lower than in control group(P<0.05),which could be partly reversed in L+P group(P<0.05).There was no significant difference in Cx37,Cx40 or Cx45 mRNA or protein expression among each group.The results of protein expression with confocal laser-scanning microscope was the same as those of Western blot.Conclusion Maternal LPS exposure during pregnancy leads to vascular changes in 6-week offspring rats,which might last until neonatal stage,and is closely correlated to hypertension in adult offsprings.

Under the different load of training stimulating, the changes in center nervous system of rat were observed.This experimental training intensities included normal -load and over-load using a swimming pool with circular water flow in order to investigate the effects on the neuron size and the density of dendritic spine of the large pyramidal cells in layer Ⅴ in motor areas of cerebral cortex.This study lead to the following conclusions:the size of nucleus and nucleolus and the density of spines of apical dendrites, insidal dendrites and basal dendrites of large pyramidal cells in layer Ⅴ in motor areas of cerebral cortex of normal-load and over-load group were higher than those of control group.It improve the capacity of brain in information processing.

BACKGROUND:The appearance of biodigradable stents brings a new dawn for the fourth coronary intervention revolution. They not only can solve the problem of postoperative acute occlusion of blood vessels, but also can be completely absorbed after a certain time.
OBJECTIVE: To summarize the application status of biodegradable coronary stents.
METHODS:PubMed, CBM and embase were searched for articles related to biodegradable intravascular stents.
RESULTS AND CONCLUSION:Biodegradable polymer stents, biodegradable magnesium stents and biodegradable iron stents are currently the three major research biodegradable stent systems. Numerous clinical trials have demonstrated the long-term safety and reliability of the biological degradation stents, and in the near future they wil replace the existing drug-eluting stents as the primary means of percutaneous coronary intervention. Biodegradable stents currently stil have their limitations, which are reflected in the relationship between mechanical properties and degradation rate and cannot be applied to complicated coronary patients temporarily. It takes 6-12 months to restore normal physiological function of blood vessels, and it can be considered reasonable that degradation of stents is completed in 12-24 months. Poly(lactic-co-glycolic) produced by polylactic acid and polyglycolic acid is currently widely recognized as the stent’s framework. We can get a more appropriate balance between the mechanical properties of the stent and the degradation rate by adjusting the ratio of polylactic acid and polyglycolic acid. This stent not only has good mechanical properties, but also can be completely biodegradable after the restoration of normal function of blood vessels, which has a broad research space.

Objective To analyze the gene expression profiles in response to ΔNp63α overexpression, and screen the potential target genes or signal pathways regulated by ΔNp63α. Methods To generate ΔNp63α overexpressed SiHa cells ( SiHa-ΔNp63α) and the control cells ( SiHa-NC) , recombinant lentivirus transfection was performed. Microarray was applied to detect the change of gene expression profiles, and the results were analyzed with bioinfor-matic software. Quantitative real-time PCR was used to validate the expression levels of selected genes. Results Among the 1405 differentially expressed genes which were statistically significant, >1. 5 fold increase or reduce of gene expression, 843 were up-regulated and 562 were down-regulated in SiHa cells with ΔNp63α overexpression. The genes were mostly involved in cell development,cycle regulation, signal transduction, communication, adhe-sion, metastasis and invasion, etc. The involved signal pathways consisted of antigen processing and presentation, cytokine-cytokine receptor interaction, cell adhesion, complement and coagulation cascades, and so on. Conclu-sion The research on the potential target genes or mediated signal pathways regulated by ΔNp63α could be helpful to explain the development of cervical cancer.