1.Correlation of alveolar bone structure and incisor labial inclination in adolescents with skeletal class II malocclusion of Uygur and Han populations in Xinjiang, China
Chinese Journal of Tissue Engineering Research 2016;20(24):3549-3554
BACKGROUND:Location relationship between maxilary incisor labial inclination and the thickness of anterior alveolar bone displays ethnic and regional differences. OBJECTIVE:To study the correlation of alveolar bone structure and incisor labial inclinationin adolescents with skeletal class II malocclusion in Uygur and Han populations of Xinjiang, China. METHODS:Sixty Uygur and 60 Han patients at 11-16 years of age (average 13.8 years old), 32 males and 28 females, with skeletal class II malocclusion wereincluded. The thickness of anterior alveolar bone and incisor labial inclination were measuredon lateralcephalometric films, and the results were analyzed using SPSS 17.0 statistical software. RESULTS AND CONCLUSION:In Han and Uygur populations, palatalalveolar bone thicknessin maxilary incisor regionwas significantly negatively correlated with the inclination of maxilary incisor (r=-0.715 for Han,r=-0.651 for Uygur,P< 0.05), while labial alveolar bone thicknessin mandibular incisor regionwas significantly positively correlated with the lower incisor inclination (r=0.902 for Han,r=0.745 for Uygur,P< 0.05). These results indicate thatthepalatal alveolar bone thicknessin maxilary incisor region decreases with the increase of the inclinationof maxilary incisor; however,thelabial alveolar bone thicknessin mandibular incisor regionincreases with the increase of the lower incisor inclination.
2.Hospital Infection among Surgical System Inpatients
Jiajia HOU ; Xiaohui RUAN ; Huilan MA
Chinese Journal of Nosocomiology 2006;0(08):-
OBJECTIVE To study the hospital infection among surgical system inpatients in order to work out more effective management plans concerning hospital infection.METHODS Totally 5963 surgical patients from Dec 2000 to Nov 2002 were analyzed by means of the retrospective study.RESULTS Among them there were hospital infections in 243 cases.The surgical hospital infection rate was 4.08% and the neurosurgical hospital infection rate was 5.37% which was the highest among all departments.The main part of infection mostly located in lower respiratory tract(21.30%),then were higher respiratory and urinary tracts(9.88%),incision site(10.70%),gastrointestinal tract(9.05%) and the others(6.17%).The infection rate in male patients was higher than in the females.The sex ratio was 2.2∶1.The infection rate was 4.67%.The highest infection rate after admission in hospital and at 31 to 60 days from January to March(4.95%) as well as from October to December(4.58%).The bacteriological rate was 28.40%,lower than the rate of≥50.00% demanded by the Ministry of Health.CONCLUSIONS The hospital infection in surgical patients is closely related to the departments,sex,duration of hospital stay,season and invasive clinical procedures.
3.Preparation and study of monoclonal antibody to matrix metalloproteinase-2
Xiaohui HU ; Fei SHEN ; Ningzheng DONG ; Peixia LI ; Changgeng RUAN
Chinese Journal of Pathophysiology 1989;0(05):-
AIM: To obtain a McAb that can inhibit the function of matrix metalloproteinase-2 (MMP-2), we expressed the fibronectin-like domain of MMP-2 (MFD) in vitro and prepared a McAb against MMP-2. METHODS: The purified MFD protein was used to immunize BALB/C mouse three times. Then the spleen of mouse was taken out and hybridized with hybridoma cells SP2/0. The positive cell clones were screened with ELISA method. The subtype and tissue specificity of the McAb were identified and its effect on endothelial cell migration and tube-formation was analyzed. RESULTS: After the spleen cells of the mouse and hybridoma cells SP2/0 were hybridized, a piece of cells that continuously secreted McAb against MMP-2 was obtained and named SZ-117. The titers of this McAb in culture supernatants and ascites were 2?10~-3 and 2?10~-5 , respectively. The heavy chain of the McAb belongs to IgG1 subclass. The McAb identified native MMP-2. MMP-2 existed in the stromal tissue of stomach, cholecystis, spleen, ovarian, prostate, salping and lymph node. It inhibited the invasion behavior of endothelial cells Eahy926 and pancreatic carcinoma cells 1990 and inhibited the tube-formation of Eahy926 cells. CONCLUSION: A useful tool for testing MMP-2 is obtained and it will be helpful to look for a kind of new anti-tumor material.
4.Adverse pregnancy outcomes among women with syphilis in Zhejiang province, 2013-2014
Xiaohui ZHANG ; Liqian QIU ; Danqing CHEN ; Lifang GUO ; Liming RUAN
Chinese Journal of Dermatology 2016;49(8):558-562
Objective To investigate the incidence of adverse pregnancy outcomes among women with syphilis in Zhejiang province during 2013-2014,and to explore the influence of anti?syphilis treatment on pregnancy outcomes. Methods A retrospective study was carried out according to the Information Management System for preventing mother?to?child transmission(PMTCT)of syphilis in Zhejiang province during 2013-2014. The incidence of adverse pregnancy outcomes was compared between women with syphilis and general women without syphilis, between treated and untreated women with syphilis, as well as between women with syphilis receiving and not receiving standard anti?syphilis treatment. The effects of anti?syphilis treatment and standard anti?syphilis treatment on pregnancy outcomes were analyzed. Results Data were collected from 3 985 women with syphilis and their children. Of these women, 12 had a stillbirth, and 3 973 a live birth. Totally, 3 210(80.55%)women received anti?syphilis treatment, 967(30.12%, 967/3 210) women received substandard treatment, and 775(19.45%)did not receive anti?syphilis treatment. Of the 3 985 fetuses or neonates born to women with syphilis, 45(1.13%)had abnormal clinical symptoms/signs. The incidence rates of low birth weight and early neonatal death were 7.00%(279/3 985) and 1.13%(45/3 985) respectively in fetuses or neonates born to women with syphilis, both significantly higher than those in fetuses or neonates born to general women without syphilis (3.18%[26 092/819494] and 0.13%[1 029/819 494], respectively, both P < 0.01). Among 775 untreated women with syphilis, the incidence rates of premature birth, low birth weight, early neonatal death, congenital syphilis and abnormal neonatal symptoms/signs were 15.61%(121/775), 10.84%(84/775), 2.45%(19/775), 3.48%(27/775)and 2.84%(22/775)respectively, all significantly higher than those in 3 210 women receiving anti?syphilis therapy (8.26%, 6.07%, 0.81%, 0.81% and 1.03%,respectively, all P < 0.01). Besides, the 967 women receiving substandard anti?syphilis treatment showed higher incidence rates of premature birth, low birth weight, early neonatal death, congenital syphilis and abnormal neonatal symptoms/signs than the 2 243 women receiving standard treatment (10.44%vs. 7.31%, 8.17%vs. 5.17%, 1.55%vs. 0.49%, 1.65%vs. 0.45%, 1.96%vs. 0.62%, respectively, all P<0.01). Conclusions The incidence of adverse pregnancy outcomes is high among women with syphilis in Zhejiang province during 2013-2014. Untreated and inadequately treated maternal syphilis is strongly associated with adverse pregnancy outcomes.
5.Effects of ferulic acid on E-selectin expression in activated endothelial cell and leukocyte-endothelial cell adhesion
Xiaolan WANG ; Xiaohui HU ; Mingen Lü ; Zhenlun GU ; Changgeng RUAN
Acta Pharmaceutica Sinica 2005;40(5):410-413
Aim To study the effects of ferulic acid (FA) on E-selectin expression in human umbilical vein endothelial cells (HUVECs) activated by lipopolysaccharide and leukocyte-endothelial cell adhesion. Methods The effects of FA on E-selectin and E-selectin mRNA expression were determined by flow cytometry and reverse transcription polymerase chain reaction. The effect of FA on HL60-HUVEC adhesion was evaluated with the method of staining the cells by Rose Bengal. Results The expression of tively). Conclusion FA can inhibit the expression of E-selectin and E-selectin mRNA and HL60-HUVEC adhesion. This may contribute to its protective effect against ischemia-reperfusion injury.
6.The relative study on snoring and hypertension in the elderly
Zhangfeng WANG ; Tao YAO ; Liying WANG ; Xiaohui ZHANG ; Jinling CAO ; Xiaolan RUAN
Chinese Journal of Postgraduates of Medicine 2013;(6):21-23
Objective To explore the relationship between snoring and hypertension in the elderly.Methods A total of 375 subjects who were ≥40 years without stroke and myocardial infarction accepted questionnaire survey on snoring.Non conditional Logistic regression analysis was used to analyze the factors of hypertension.Results In 375 subjects,non-snore was in 169 subjects,occasionally snore was in 86 subjects and regular snore was in 120 subjects,the prevalence of hypertension in them were 33.7%(57/169),39.5%(34/86) and 49.2% (59/120),respectively,there was significant difference among them.Non conditional Logistic regression analysis showed:after correcting other factors,regular snore was still risk factor of hypertension,the OR value was 2.124 (95% CI 1.189-3.796).According to gender stratification,regular snore was still risk factor of hypertension in female,the OR value was 3.993 (95% CI 1.259-12.663),rather than risk factor in male.Condusion Regular snore is relevant to the increased prevalence of hypertension,and it is a risk factor of hypertension.
7.Effect of diltiazem on heme oxygenase-1 and nitric oxide synthase in rats with pulmonary hypertension
Ying CHEN ; Yingmao RUAN ; Li LI ; Yan CHU ; Xiaomei ZHOU ; Xinlin XU ; Lianzhuang ZHNAG ; Wenxue SI ; Xiaohui LI
Chinese Journal of Pathophysiology 1989;0(05):-
AIM: To investigate the action of diltiazem (a calcium antagonist) on the expression of heme oxygenase (HO) -1 and nitric oxide synthase (NOS) in the small pulmonary arteries (SPA) of rat in chronic hypoxia. METHODS: Chronic pulmonary arterial hypertension models were established by treating the rats in hypoxic environment[(10%?1%)O 2] for 6 weeks. After 2 weeks of hypoxia, rats were treated with diltiazem (15 mg/kg/day). Right ventricular systolic pressure (RVSP) and right ventricular hypertrophy index (RVHI) were measured. Pathological changes in the lungs were observed under the light microscope and transmission electron microscope. The expression and distribution of heme oxygenase (HO) -1, endothelial NOS (eNOS) and inducible NOS (iNOS) were tested by immunohistochemistry and Western blot. Guanosine-3', 5'-cyclic monophosphate (cGMP) of lung tissues were detected with radioimmunoassay. RESULTS: Diltiazem significantly decreased abnormal RVSP, and RVHI in model rats, attenuated the SPA media thickeness, and recovered abnormal eNOS and iNOS expression in SPA. Whereas diltiazem had little effect on the increased HO-1 expression in SPA caused by hypoxia and ultrastructure injury in endothelium. cGMP levels were corresponded with HO-1. CONCLUSION: Diltiazem has a significant effect on inhibiting hypoxic pulmonary hypertension structural remodeling. These effects might be partly attributed to the suppression of iNOS, promotion of eNOS, and not attenuation HO-1 expression in the lung of hypoxic rats.
8.Association study between single nucleotide polymorphism in AOAH gene and chronic rhinosinusitis in a Chinese population.
Yanming ZHAO ; Yuan ZHANG ; Xiaohui CUI ; Yu RUAN ; Luo ZHANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2014;49(8):668-674
OBJECTIVETo replicate the polymorphisms in risk genes of chronic rhinosinusitis in a Chinese Han population.
METHODSEnrolled in this study were CRS patients with nasal polyps (n = 306, CRSwNP), CRS patients without nasal polyps (n = 332, CRSsNP), and controls (n = 315) in a Chinese population. All the patients were recruited from clinic of the department of Otorhinolaryngology of Beijing Tongren Hospital between 2008 February and 2009 July. A total of 10 single nucleotide polymorphisms (SNPs) selected from previous identified SNPs associated with CRS in Canadian population were individually genotyped. Allele and genotype frequencies were calculated by frequency counting, the chi-square test or exact method were applied to analyze the results.Final results were corrected by Bonferroni multiple correction.SPSS 13.0 software was used for statistical analysis.
RESULTSOne SNP in AOAH gene(rs4504543, P = 1.95 × 10⁻⁵, OR = 0.559 0) was identified to be significantly associated with whole CRS cohort. After subgroup analysis for the presence of nasal polyps (CRSwNP and CRSsNP), the same SNP in AOAH (rs4504543, P = 3.47 × 10⁻¹², OR = 0.284 8) was also found to be significantly associated with CRSsNP cohorts.
CONCLUSIONSAOAH was significantly associated with CRS and its polymorphisms might play a role in the susceptibility to develop CRS in Chinese population.
Alleles ; Asian Continental Ancestry Group ; statistics & numerical data ; Canada ; epidemiology ; Chronic Disease ; Genotype ; Humans ; Nasal Polyps ; Polymorphism, Genetic ; Polymorphism, Single Nucleotide ; Rhinitis ; epidemiology ; genetics ; Sinusitis
9. Prognostic significance of early assessment of minimal residual disease in acute myeloid leukemia with mutated NPM1 patients
Ting ZHAO ; Honghu ZHU ; Jing WANG ; Jinsong JIA ; Shenmiao YANG ; Hao JIANG ; Jin LU ; Huan CHEN ; Lanping XU ; Xiaohui ZHANG ; Bin JIANG ; Guorui RUAN ; Debing WANG ; Xiaojun HUANG ; Qian JIANG
Chinese Journal of Hematology 2017;38(1):10-16
Objective:
To explore prognostic significance of early assessment of minimal residual leukemia (MRD) in adult patients with
10. Detection and analysis of plasma VWF levels in healthy Chinese by automatic standardized assay
Ping ZHANG ; Ziqiang YU ; Xiaohui ZHANG ; Wei ZHANG ; Xia BAI ; Lijuan CAO ; Jian SU ; Pingping SHA ; Zhaoyue WANG ; Changgeng RUAN
Chinese Journal of Hematology 2017;38(2):146-152
Objective:
To explore the normal range of plasma VWF levels of healthy Chinese and to analyze the influencing factors to VWF level.
Methods:
To detect the levels of von Willebrand factor antigen (VWF∶Ag) , von Willebrand factor ristocetin cofactor activity (VWF∶Rco) , von Willebrand factor collagen binding activity (VWF∶CB) , and the factor Ⅷ coagulation activity (FⅧ∶C) by using fully automatic and standardized testing instruments and matching reagent in 70 healthy Chinese. The effects of age, ABO blood type, gender and region were also analyzed. Meanwhile, 8 standard plasma samples (2 normal subjects, 6 cases of type 2 VWD) confirmed by NIBSC were tested for VWF values.
Results:
① In 70 cases of healthy Chinese, the mean value of plasma VWF∶Ag, VWF∶Rco and VWF∶CB were (95.4±44.9) %, (105.9±35.4) % and (89.8±28.4) %, respectively; the ratio of VWF∶Rco/VWF∶Ag and VWF∶CB/VWF∶Ag was 1.18±0.25 and 1.03±0.29, respectively. ②There was no statistical significance in plasma VWF values between the age ≥30 years and <30 years group (