Objective To study the biological effects of nucleic transcript factor NF-?B/RelB gene silencing on bone marrow derived dendritic cells(BMDCs).Methods For constructing a new type of DCs,RNAi RelB DC,mouse BMDCs were cultured through IL-4 and GM-CSF cytokine inducing system and were purified with CD11c positive immunomicrobeads by flow cytometer,and RelB gene was silenced with PCR expression cassettes.The RelB RNA interference effect in DCs was proved at both mRNA and protein levels.The biological characteristics of RNAi RelB DCs were investigated by means of cell morphology,cell surface markers and mixed lymphocyte reaction ability.The cell microstructure was observed using scanning electron microscopy and transmission electron microscopy.The cell surface markers,such as MHC-Ⅱ,CD86 and CD40,were detected by flow cytometer.The mixed lymphocyte reaction ability,as well as the ability of DCs in stimulating allogeneic T cells proliferation,was detected by 3H-TdR incorporation method.Results RNAi RelB DCs seemed to have fewer and shorter spikes and many folds under scanning electron microscopy.Many phagocytic vacuoles were observed in the cytoplasm under transmission electron microscopy.DCs surface molecules,MHC-Ⅱ,CD86 and CD40,were expressed at a relatively lower level compared with the control DCs.This RNAi RelB DCs also showed weak allogeneic T cells stimulating ability in vitro in MLR.Conclusion The dendritic cells derived from bone marrow are characterized the biological features and immunological function of immature DC with silencing of RelB gene.This kind of RNAi RelB DCs may be used to induce immunotolerance as a novel tolerogenic DCs.

To understand the condition of nosocomial infection in Jilin City, and to estalish a perfect monitoring system of hospital infection, the Departments of Internal Medicine and Surgery were randomly selected in three hospitals.Among the 1359 cases monitored, 228 were found to be nosocomial infection patients, the total rate was 16.80%. The infection rate in Department of Respiratory was the highest (18.63%). The sites of nosocomial infection were deep resiratory tract(38.60%), urinary tract(26.32%), operation-wound(17.11%),etc. The main pathogenic bacteria were G - bacilli(61.20%), most common one was Pseudomonas aeruginosa(25.60%) and the bacteria were resistant to many kinds of antibiotics. antibiotics abuse was quite often. It is necessary to establish the monitoring system of nosocomial infection and take measures to reduce its incidence.

2 weeks(OR=9.6513),operation duration≥4h (OR=7.3154),combined use of antibiotics(OR=8.9698), lowering of immunity (OR=6.8861),use of cortical hormone(OR=6.5896),prophylactic therapy for stress ulcer (OR=12.6371), retention of nosogastric tube (OR=9.9514) were risk factors.It was suggested that there was a retrograde route of infection from the stomach to throat and then lower respiratory tract, and NP was the result of multiple risk factors.

Objective To investigate the effectiveness of microresection combined with radiofrequency ablation under laryngoscope and rigid telescope for laryngeal precancerosis. Methods Fifty-one patients with laryngeal precancerosis, which was diagnosed pathologically, were treated with microresection combined with radiofrequency ablation under laryngoscope and rigid telescope. The patients were followed up for over 6 months. Results During a mean of 26-month follow-up (6 months to 4.5 years), 7 of the patients had recurrence (vocal cord leukoplakia in 3, atypical hyperplasia in 2, and laryngeal papilloma in 2); 4 had carcinomatous change (vocal cord leukoplakia in 1, atypical hyperplasia in 2, and laryngeal papilloma in 1);and 2 patients developed laryngeal web after surgical treatment for bilateral vocal cord leukoplakia. One month after the operation, the Jitter, Shimmer, and NNE were decreased significantly (P

AIM:[WTBZtive of this study was to examine the influence of different flow pattern, particularly low shear stress flow regimens, on VCAM-1 and NF-?B in vascular endothelial cells. [WTHZ] METHODS: HUVEC covered sheets were placed within rectangular parallel plate flow chambers. Cells were subjected to 4, 18 h of either laminar or oscillatory shear stress. The expression of VCAM-1 and NF-?B activity were analyzed by immunohistochemistry. VCAM-1 mRNA was also detected using in situ hybridization. RESULTS: VCAM-1 mRNA and VCAM-1 expression were not upregulated by laminar shear stress. However, oscillatory shear stress significantly upregulated VCAM-1 mRNA and VCAM-1 expression. Translocation of NF-?B P65 was remarkably increased. CONCLUSION: In same low shear stress, the different expression of VCAM-1 mRNA and VCAM-1 were induced by HUVEC exposed to laminar or oscillatory flow patterns.

OBJECTIVETo investigate protective effect of paeonol (Pae) on human umbilical vein endothelial cells (HUVECs)injured by hyperlipidemic serum and explore its possible mechanism.METHODS The injury model was induced by 20％ hyperlipidemic serum incubating HUVECs for 24 h.Pae 124,247 and 495.μmol· L-1 were given followed by administration of hyperlipidemic serum for 24 h.The morphological changes were observed under inverted microscope,cell survival rate was evaluated by MTT method,the nitric oxide (NO) content was measured by nitric acid reductase method and the endothelial nitric oxide synthase (eNOS) mRNA expression was determined by RT-PCR.RESULTSCompared with normal contorl group,most cells in model group split and exfoliated.However,the morphology was tending to normal level after intervention with Pae.Pae significantly improved cell viability(P ＜0.01).Compared with model group,the survival rate increased from (53.0 ±10.1 ) ％ to ( 68.4 ± 9.1 ) ％,( 84.5 ± 6.7 ) ％ and (98.1 ± 7.5 ) ％,respectively.The NO content and eNOS mRNA expression both increased greatly in Pae groups(P ＜ 0.01 ).Compared with model group,content of NO increased from 54 ± 4 to 79 ± 6,115 ± 5 and ( 136 ± 6) μmol · L- 1,respectively.The expression level of eNOSmRNA improved from 0.215 ± 0.060 to 0.451 ± 0.045,0.563 ± 0.013,0.704 ± 0.068,respectively.CONCLUSIONPae exerts protective effect on HUVECs injured by hyperlipidemic serum by increasing eNOS mRNA expression,which might therefore improve the content of NO.

In a hospital, key disciplines are often regarded as the representative of service and reputation of the hospital. It is therefore of great significance to strengthen the construction of key disciplines to ensure sustainable development of the hospital in the increasingly fierce competition of medical market. This paper discusses how to strengthen construction of key disciplines from four aspects, namely, to increase the awareness of focal discipline construction among hospital staffs, and establish "brand awareness" ;to develop a team of high level professionals, and develop or introduce academic leaders; to improve academic research level and promote the technology progress ;and to strengthen management by objectives.

AIM: To observe the effect of MM-LDL (minimally modified LDL) on the interaction between human umbilical vein endothelial cell (HUVEC) and U937 monocyte-like cell line and the exporession of vascular cell adhesion-1 (VCAM-1), intercellular adhesion molecule-1(ICAM-1), P-selectin.METHODS:The adhesive percentage between HUVEC treated with MM-LDL and U937 was determined by counting and the expression of VCAM-1, ICAM-1, P-selectin were examined by ELISA. RESULTS: Treatment of HUVEC with MM-LDL (75 mg/L) for 4 hours significantly increased adhesion of U937 to HUVEC ( P

Objective To analyse the reasons for patients′long PICU stay and to discuss how to de-crease their PICU stay length and optimize medical resource utilization.The another objective is to identify these patients′long-term outcome.Methods We retrospectively analysed the basic information of patients who stayed in our PICU more than 30 days during January 1,2013 to July 31,2014,including pediatric criti-cal illness score on admission,primary diseases and outcome on discharge,in order to determine main reasons for their long PICU stay.Follow-up prognosis was made by Paediatric Overall Performance Category.We used SPSS 19.0 to do data analysis.Results Sixty-seven patients enrolled in this study included 43 boys and 24 girls,49.2%of whom came from emergency department.The median age was 24 months(IQR 8,108), while median length of PICU stay was 39 days(IQR 31,49).Mean pediatric critical illness score on admi-ssion was 78.39 ±9.57.Mean hospital fees was(144 071.74 ±76 944.74)Yuan.Thirty(44.8%)patients did not have any medical insurance.Top three primary diseases were respiratory disease(30 cases),neurolog-ical disease(8 cases)and systemic infection(6 cases).Top three underlying diseases were tracheostenosis or bronchopulmonary dysplasia(6 cases),congenital heart disease(5 cases)and immunodeficiencies(4 cases). Top three complications were respiratory failure(30 cases),multiple organ dysfunction or failure(16 cases), shock(9 cases).Top three reasons for longer PICU stay were weaning off mechanical ventilation difficulty (29 cases),department or hospital transference difficulty(20 cases),feeding difficulty(16 cases).Fifty-four patients were discharged with better health condition,10 patients were auto-discharge.The mortality at dis-charge,1 month after discharge,3 months after discharge and 6 months after discharge were 4.5%,20.9%, 25.4% and 25.4%,respectively.Total 36(53.7%)patients had a good or mildly abnormal Paediatric Over-all Performame Category score(1-2 points)at 6 months after discharge.Conclusion Sequential noninvasive ventilation,timely tracheotomy,timely patients′transference to general ward,development of family ward and rehabilitation wards,appropriate nutrition delivery,regularly difficult disease consultation,reduction of hospi-tal infection and complications may do contribution to shorten these patients′length of PICU stay.Children who survive longer than 3 months after discharge have better long-term prognosis.

Objective To investigate the time and dose dependent effects of 17 β-estradiol ( E2 ) on eNOS phosphorylation and nitric oxide (NO) production from bovine aortic endothelial cells (BAECs). Methods The BAECs were cultured in 24-well flat plate.The dose dependent rapid induction of NO release by E2 in the BAECs was explored,and the non-genomic mechanism was studied. Each experiment was repeated for 3 times. The NO level was quantified by the electron spin resonance spectroscopy(ESR)method,and the phosphorylation of eNOS were determined by Western blot. Results NO release increased time dependently from BAECs after treatment with E2 at 100 nmol?L-1 at 1,5,10 and 15 min,and the effect peaked at 10 min.There were dose dependent effects of NO production as well as eNOS phosphorylation after treatment with E2 at different concentrations for 10 min,and the effect was the most obvious at the concentration of 100 nmol?L-1 .Upon treatment with equal volume of saline,E2 and actinomycin D (25 μg?mL-1 ) for 10 min,the NO release was(5.38±2.35),(10.59±3.28)and(10.68± 3.31) nmol?mg-1 ,respectively,and the eNOS phosphorylation level was 0.36±0.03,0.98±0.08 and 0.99±0.08,respectively. Compared with 0.9% sodium chloride solution,the NO release and eNOS phosphorylation were significantly increased in E2 treated cells(all P<0.05). Conclusion 17 β-estradiol at 100 nmol?L-1 induced eNOS phosphorylation as well as NO production from bovine vascular endothelial cells through non-genomic mechanism.The effect peaked at 10 minutes.