Objective To observe the effect of panax notoginseng saponins on pulmonary artery pathology in chronic hypoxic pulmonary hypertensionp (HPH) rats models, discuss the role and possible mechanisms of panax notoginseng saponins in prevention of chronic hypoxic pulmonary hypertension.Methods Forty male Sprague-Dawley (SD) rats were randomly divided into four groups (n =10).Group C: control group;Group H: rats were treated with hypoxia only;Group HC: rats were treated with hypoxia and captopril;and Group HP: rats were treated with hypoxia and panax notoginseng saponins.To observe the effect of panax notoginseng saponins prophylactic treatment in chronic hypoxic pulmonary hypertension rats,after the establishment of the model of hypoxic rat animal pulmonary hypertension models, transthoracic direct pulmonary artery intubation was measured in rat pulmonary arterial systolic pressure and mean pulmonary artery pressure, at the end of separation and cut for pulmonary artery to observe the pathological changes of pulmonary artery, results were recorded and statistically analyzed.Results (1)Transthoracic direct pulmonary artery intubation was measured in rat pulmonary arterial systolic pressure and mean pulmonary artery pressure: pulmonary artery systolic pressure (PASP) and mean pulmonary arterial pressure (MPAP) were significantly higher in Group H than in Group C (P ＜ 0.05), Group HP was no significant difference relative to Group C.(2)Pathological section shew that the primary pathological change of group H is vascular intimal hyperplasia and the proliferation of smooth muscle cells, medial hypertrophy, extracellular matrix increased, thickening of the vessel wall and the vascular stenos.Conclusions Panax notoginseng saponins inhibit the formation of chronic hypoxic pulmonary hypertension and pulmonary vascular remodeling in rats,and have the effect of prevention and treatment of pulmonary hypertension.

0. 05); The long-term effective rate was 84. 21% in the treatment group and 54. 55% in the control group with a significant difference between the two groups (P

Objecfive To investigate the prevalence of Ureaplasma urealyticum(UU)and Mycoplasma hominis(MH)infections in urogenital tract and their sensitivities to drugs in Qinhuangdao area from 2006 to 2007.Methods UU and MH were detected by Mycoplasma IST kits in urine samples from 3062 patients suspected as urogenital tract infection and the sensitivity tests for 8 antimicrobial agents were performed.Results Mycolasmas were detected in 1262 patients from 3062 patients suspected as urogenital tract infection,in which 1037 were of UU infection,41 were of MH infection and 184 were of UU+MH infection.Both strains were sensitive to josamycin,doxycycline and tetracycline,but were less sensitive to ofloxacin and ciprofloxacin.Conclusions Drug resistance is increasing for Mycoplasma and sensitivity test is important in treatment of urogenital tract infection by Mycoplasma.

Objective To compare the effects of penequinine hydrochloride and atropine on the cardiovascu-lar stability about preoperative in children by monitoring and recording the HR, MAP, SpO2, ECG changes. Methods 40 children underwent selective operation were randomly divided into two groups, 20 in each group. Penequinine hydrechloride group(group A) and atropine group(group B) were injected intramuscularly with penequinine hydro-chloride 0. 015mg/kg or atropine 0. 015mg/kg respectively, at 30 minutes before operation. Patients' HR, MAP, SpO2 and ECG changes were recorded at 10min,20min,30min and 40min after injection. Results The HR,MAP,SpO2 levels of group A and SpO2 level of group B had no significant changes. There are significant changes in HR,MAP of group B. Conclusion Penequinine hydrochloride can be used as preoperative medication and it is conducive to the maintenance of cardiovascular stability.

A clinical emergency skill contest using emergency care simulator (ECS) was carried out　on May 2011.Total 30 family physicians in 10 teams from Shenzhen community health centers participated in　the contest.The performance and the scores of each item were analyzed.The overall score was 61.3 ± 11.9.The average score for general treatment was 33.0 ± 6.9 with a score rate of 66％ (33/50) ; the average　score for cardiopulmonary resuscitation was 23.2 ± 7.2 with a score rate of 57.5％ (23/40) ; the average　team score was lowest (5.0 ± 1.1 ) with a score rate of 50.0％ (5/10).The results indicate that family　physicians should strengthen clinical skill training of emergency care,particularly in on-site response and　teamwork.

Liver cancer remains one of the leading cause of cancer death in the world.Animal models, especially mouse models, are important tools for studying the biological characteristics, pathogenesis, new drug screening and therapy of liver cancer.Up to now, although the development of various animal models accelerates the research of liver cancer, all the existing models have their own disadvantages.Lacking of economical and applicable animal models that can mimic the human liver cancer seriously restrict the further study of liver cancer.With the development of genetically modified technologies, it provides a fast, easy and reliable method to establish liver cancer models.In this review, we describe the different types of mouse models used in liver cancer research, with emphasis on genetically engineered mice used in this field, which may open an avenue for functional cancer genomics and generation of liver cancer models by using gene editing technologies.

The 2016 conference on iodine and thyroid disease is aimed to establish a platform for sharing the achievement on fundamental,preventive and clinical research of iodine and thyroid disease,for exchanging information between producer and consumer on iodized salt,iodine additive and iodine determination,for communication on policy and measures concerning iodine deficiency disorders (IDD) prevention and control,for promoting the exchange of experience between international and domestic scholars in IDD prevention and control and the clinical,fundamental research update and achievement,and for discussing the key and difficult problems.After communication and discussion,the participants at the 2016 conference drafted a consensus on iodine and thyroid disease.Through incorporating the current IDD prevention and control schedule and task,this article describes,elaborates and explains the key items of the consensus with expectation of a better utilization and application of the consensus in the future in IDD prevention and control.

ObjectiveTo investigate the value of CD26/DPPⅣ or galectin-3 immunohistochemical staining and their combination in the diagnosis of thyroid carcinoma. MethodsEnVision immunohistochemical technique was applied to detect the expressions of CD26/DPPⅣ and galectin-3 in thyroid tissue of 114 cases with benign or malignant thyroid tumors. ResultsCD26/DPPⅣ and galectin-3 were expressed in most of papillary carcinomas but absent in normal thyroid tissues, most of follicular carcinomas and thyroid adenomas. With CD26/DPPⅣ as means to detect papillary carcinoma, the sensitivity, specificity, diagnostic accuracy, positive predictive value, negative predictive value and Kappa index were 86.8%, 97.2%, 90.4%, 98.3%, 79.5% and 0.80 respectively,andwithgalectin-3therespective figures were 97.1%, 91.7%, 95.2%, 95.7% , 94.3% and 0.89. With CD26/DPPⅣ and gelectin-3 combined, the sensitivity, specificity, Kappa index and others in papillary carcinoma were the same as those of galectin-3 in parallel test, and the same as those of CD26/DPPⅣ in serial test. ConclusionBoth CD26/DPPⅣ and galectin-3 are reliable markers of papillary carcinoma, CD26/DPPⅣ or galectin-3 immunohistochemical staining can be used as a supplement for conventional pathological diagnostic approach to differentiate thyroid papillary carcinomas from adenomas, while the application in the diagnosis of follicular thyroid carcinoma remains to be further investigated.

The levels of protein expressions o f insulin receptor substrate-1 and -2 (IRS-1 and IRS-2) in abdominal subcuta neous adipose tissue from type 2 diabetic patients were measured by Western blot technique. The expression of IRS-1 protein was reduced and the expression of I RS-2 protein was unchanged in adipose tissue of type 2 diabetic patients. IRS- 2 may be the main docking protein and one of the factors causing hyperinsulinemi a and insulin resistance in type 2 diabetes mellitus.

Objective To study the effects and the mechanism of the ethanol extract of Blueberry(BE) on relaxation vascular endothelium or smooth muscle.Methods To use rat aorta as the specimen,to observe the effects of BE on induced relaxation of the phenylephrine-precontracted aorta.and approach the mechanism on vascular endothelium or smooth muscle.Results BE induced relaxation of the phenylephrine-precontracted(1.0×10~(-5)mol·L~(-1) aorta in a dose-dependent way(P<0.01),which was disappeared by removal of functional endothelium(P<0.01).Pretreatment of the aortic tissues with NG-nitro-L-arginine methyl ester(L-NAME),methylene blue,or 1H[1,2,4]-oxadiazole-[4,3_a]-quinoxalin-1-one (ODQ) inhibited the vascular relaxation induced by BE(P<0.01).BE-induced vascular relaxations were also markedly attenuated by addition of verapamil or diltiazem,while the relaxant effect of BE was not blocked by pretreatment with indomethacine,glibenclamide,tetraethylammonium(TEA),atropine,propranolol(P<0.01).Conclusion These results suggest that BE dilates vascular smooth muscle via endothelium-dependent nitricoxide-cGMP signaling pathway,possible involvement of L-type Ca~(2+) channel.