BACKGROUND:Stem cel transplantation is a promising treatment for type 1 diabetes mel itus. Adipose-derived mesenchymal stem cel s have become another hotspot fol owing bone marrow mesenchymal stem cel s.
OBJECTIVE:To investigate the therapeutic effect of adipose-derived mesenchymal stem cel transplantation on type 1 diabetes in a rat model.
METHODS:Forty-five Sprague-Dawley rats were randomized into normal, model and cel transplantation. Animal model of type 1 diabetes was made in the latter two groups through intraperitoneal injection of streptozotocin. Seven days after modeling, rats in the three groups were given intraperitoneal injection of normal saline, serum-free DMEM or adipose-derived mesenchymal stem cel suspension, respectively. Two weeks after injection, body mass, blood glucose level, insulin secretion and PDX-1 mRNA in the pancreatic tissue of rats were monitored and detected in the three groups.
RESULTS AND CONCLUSION:After modeling, the body mass of rats were lowered, and increased gradual y in the cel transplantation group at 2 weeks after cel transplantation, but it was stil decreased in the model group. Compared with the normal group, the fasting blood glucose level was significantly higher in the model group (P<0.05), but it was reduced significantly after cel transplantation (P<0.05). Compared with the normal group, the insulin level was reduced significantly in the model group (P<0.05), but it was increased significantly after cel transplantation (P<0.05). Highest and lowest PDX-1 mRNA expressions were obtained in the normal and model groups, respectively;and there was a significant difference between groups (P<0.05). Al these findings show that adipose-derived mesenchymal stem cel transplantation relieves hyperglycemia in rats by promoting the expression of PDX-1 in the rat pancreatic tissue.

Objective To study the effects of early rehabilitation with stroke patients by applying Snyder hope theory in clinical nursing. Methods A total of 76 acute stroke patients from Henan Province Hospital of Traditional Chinese Medicine were divided into two groups by random digits table method:experimental group and control group, 38 cases in each group. The final effective cases were 72 and 36 cases in each. The control group received routine care, while those in the experimental group accepted nursing intervention with Snyder hope theory based on routine puncture care for 4 weeks. Herth Hope Index(HHI), National Institute of Health Stroke Scale(NIHSS), Barthel Index(BI) and adherence score before and after 4 weeks were evaluated and compared between 2 groups. Results Before intervention and 4 weeks after intervention, the experimental group of patients with hope level score respectively (31.61 ± 1.95), (40.67 ± 4.01) points, NIHSS scores respectively (18.27 ± 5.31), (10.41 ± 4.21) points, BI scores respectively (37.19 ± 3.31), (46.12 ± 3.51) points, and adherence of functional exercise scores (0.97 ± 0.31), (2.81 ± 0.52) points. The differences were statistically significant (t=17.765-52.571, all P<0.01). There were statistically significant differences with hope level, NIHSS score, BI score before intervention and 4 weeks after intervention in the control group (t=5.435, 4.929, 11.067, all P<0.01), except for adherence of functional exercise scores (t=1.220, P=0.231) in the control group, but the change not as the experimental group. Compared with the control group, there were statistically significant in the score of hope level, NIHSS score, BI score and adherence of functional exercise with the experimental group after intervention ( t=6.030-10.690, all P<0.01). Conclusions The nursing with Snyder hope theory can promote the progress of early rehabilitation of acute stroke patients as well as the patients′exercise adherence.

Danggui Injection and Vitamin B12 Injection were injected into acupoints Geshu (BL17), Pishu (BL 20), Zusanli (ST 36), Fenglong (ST 40) and Neiguan (PC 6) to treated 56 cases of hyperlipidemia. Meanwhile, Fenofibrate was adopted to treat another 45 cases as control. After2-month treatment, result showed similar effects in the two groups.

Objective To predict the infarction of related artery(IRA)and the site of occlusion by analyzing the diversify of electrocardiographic ST segment and coronary angiography findings in acute inferior myocardial infarction. Methods Sixty-four patients with acute inferior myocardial infarction were divided into two groups by coronary angiography: right coronary artery(RCA)occlusion group(51 patients)and left circumflex coronary artery(LCX)occlusion group(13 patients). RCA occlusion group included proximal,middle and distal components occlusion, and LCX occlusion group included proximal and distal components occlusion. The cases of STⅢ elevation ＞ ST Ⅱ ,STaVL depression ＞ STⅠ ,STV1 depression,STV7-V9 and STV3R-V5R elevation were recorded and compared. Results The percentage of STⅢ elevation ＞ STⅡ, STaVL depression ＞STⅠ, STV1 no depression, STV7-V9 no elevation and STV3R-V5R elevation was significantly higher in RCA occlusion group than those in LCX occlusion group(P ＜ 0.01 or ＜ 0.05). The percentage of ST Ⅲ elevation ≤ ST Ⅱ, ST,VL depression ≤STⅠ ,STV1 depression,STV7-V9 elevation,STV3R-V5R no elevation was significantly higher in LCX occlusion group than those in RCA occlusion group(P ＜0.01 or ＜0.05). In RCA occlusion group:the sensitivity of STⅢ elevation ＞ STⅡ, STaVL depression ＞ STⅠ and STV1 no depression was 90.2%, 80.4% and 80.4%;the specificity of STⅢ elevation ＞ STⅢ and STaVL depression ＞ STⅠwas 84.6% and 84.6% ;the sensitivity of STV3R-V5R elevation was 51.0%, but its specificity was 100.0%. In LCX occlusion group: the sensitivity of STⅢ elevation ≤ ST Ⅲ and STaVL depression ≤ ST Ⅰ was 84.6% and 84.6%, and their specificity was 90.2% and 80.4%;the specificity of STV1 depression and STV7-V9 elevation was 80.4% and 78.4%; the sensitivity of STV3R-V5R no elevation was 100.0% ,and its specificity was 51.0%. In RCA occlusion group, the percentage of STV3R-V5Relevation was higher in proximal components occlusion than that in distal components occlusion[76.9%(10/13)vs. 27.3%(3/11),P=0.015]. Conclusions The IRA can be initially judged by analysis of the characteristics in the ST segment in Ⅰ,Ⅱ ,Ⅲ ,aVL,V1,V7-V9,V3R-V5R lead in acute inferior myocardial infarction. And the ST segment elevation in V3R-V5R is meaningful in the judgment of proximal and distal components occlusion in RCA.

OBJECTIVE:To investigate the characteristics and general regularity of ADR induced by Danhong injection,and to provide reference for rational drug use in the clinic. METHODS:Using“Danhong injection”and“ADR”as keywords,retrieved from CJFD,literatures published during 2005-2015 were collected and analyzed statistically in repsects of patient’s gender and age,disease and allergic history,drug combination,occurrence time of ADR and clinical manifestations,etc. RESULTS:A total of 24 literatures were collected,involving 360 cases. Female patients were more than male(66.11% vs. 33.89%);ADR often oc-curred within 30 min during medication(169 cases,46.94%);main clinical manifestations were lesion of skin and its appendents (163 cases,42.89%), systemic injury(72 cases,18.95%). The severe one would suffer form allergic shock. CONCLUSIONS:Great importance should be attached to the indication of Danhong injection,treatment based on syndrome differentiation,rational drug use and close monitoring. Constant vigilance is necessary to the occurrence of ADR.

In order to observe the therapeutic effect of Eight Battle Points for simple obesity.Methods:In the treatment of 98 cases of the patients with obesity by the oblique puncture of long needles into Eight Battle Points,the body weight,body weight index (BWI),waist circumference,buttock circumference and fat distribution rate of the patients were measured and assessed respectively before and after the treatments.And the changes of complications of obesity were observed before and after the treatments.Results:There were significant differences (P＜0.05) in various indexes of obesity before and after acupuncture treatment.Conclusion:Acupuncture by puncturing Shenque(CV 8) plus Eight Battle Points is positive in the treatment of obesity and has a better therapeutic effect for complications of obesity.

Objective To observe the analgesic effect of progressive muscle relaxation training (PMR) and compound lidocaine cream in hemodialysis patients undergoing puncturing on arteriovenous fistula.Methods 96 hemodialysis patients undergoing puncturing on arteriovenous fistula were randomly divided into the progressive relaxation training group (group A),compound lidocaine cream group (group B),progressive relaxation training and compound lidocaine cream group (group C),and the blank control group (group D),with 24 patients in each group.Compound lidocaine cream were applied on the central puncture point 60 minutes before operation in group B and group C,and the patients of group A and group C were conducted with progressive relaxation training before puncture and sleep,while no intervention were assigned to group D.The pain of each patients were measured in 10 minutes with Visual Analog Scale(VAS).Results The pain scores were respectively (3.042 ± 0.999) in group A,(2.500 ±0.978)in group B,(1.333 ± 0.817) in group C and (5.042 ± 1.122) in group D.The AVOVA under the significant level of 0.05 for factorial date indicated that the main effect of factor A (progressive relaxation training) was statistically significant and the main effect of factor B (compound lidocaine cream) was also statistically significant.And that statistically significant interaction between the two factors was found,too.Conclusions Compound lidocaine cream with progressive relaxation training could alleviate the pain during puncturing arteriovenous fistula from hemodialysis significantly and was better than single use of each method.

Objective To observe the changes of endothelin and 6 Keto PGF 1? of patients with pulmonary heart disease and effects of Compound Danshen,to study the role of ET 6 Keto PGF 1? in pulmonary heart disease and pulmonary artery hypertension,and explore a safe and effective drug for pulmonary artery hypertension Methods 40 patients with CPHD and 30 patients with COPD as well as 20 healthy persons as control whose levels of plasma ET and 6 Keto PGF 1? were measured by radioimmunization,meanwhile,PaO 2 was measured by blood gas analyze instrument Results The level of ET in CPHD was higher than that in COPD and healthy group,company with the lower level of 6 Keto PGF 1? After treatment with Compound Danshen,the level of ET was reduced and 6 Keto PGF 1? was elevated Conclusions The balance between ET and PGI is important in modulating pulmonary circulation Compound Danshen can reduce pulmonary artery tension through protecting endothelium,decreasing ET,and increasing PGI 2

Objective To compare the effect of low-temperature plasma ablation and YAG laser treatment on persistent allergic rhinitis(PAR) after septoplasty and to seek adjunct surgical procedures to tackle PAR.Methods Follow-up was performed three years after randomized grouping on PAR patients ( Low-temperature plasma ablation group 127 cases and YAG laser group 1 18 cases).Visual Analog Scale (VAS) was used to evaluate symptoms,such as nasal congestion,running nose,sneezing,nasal itching,eye itching etc preoperatively and postoperatively in order to compare the treatment effectiveness and intergroup difference.Results The efficacy rate of Low-temperature plasma ablation group was 87.4％,and YAG laser group was 66.1％.The difference was statistically significant( x2 =15.74,P ＜ 0.01 ).The VAS scores for postoperative symptom relief in both groups were significantly different ( t =21.24,20.56,all P ＜ 0.0 1 ).Conclusion As an adjunct treatment approach,low-temperature plasma ablation was superior to YAG laser in the treatment of PAR with preferable safety.

Hyperplastic disease of the breast (HDB) is caused by a hormone imbalance experienced among women at a certain age. Slight breast pain is common in women before menstruation without need of treatment; however, if the pain becomes severe, it can cause physical and mental suffering. Therefore, it is of great clinical significance to control this disease.