Objective To evaluate the relationship between ischemic cerebrovascular desease(ICVD) and carotid atherosclerosis and its risk factors.Methods 186 cases with ICVD and 194 cases without cerebrovascular disease or healthy people(control group) were enrolled in this study.Carotid ultrasonagraphy was performed in the two groups and blood biochemical indexes were detected in the meanwhile.The data were compared between the two groups.Results Higher age(69?7years) and higher prevalence of hypertension(66.1%),diabets mellitus(53.4%) and metabolic syndrome(44.6%) were found in ICVD group than in control groups [(61?5)years,48.8%,15.2%,12.9%](all(P

Objective To study the inhibitory effect and mechanism of fluorofenidone on the proliferation of the rat lung fibroblasts induced by TGF-β1 . Methods Pulmonary fibroblasts cell were cultured and purified by using differentially adherent method, the third generation was used in the experiment. Cells were pretreated with 1,3,10 mmol?L-1 fluorofenidone, respectively. Pulmonary fibroblasts were identified by immunocytochemistry, and interfered by transient transfection. Cell proliferation was measured by BrdU marking. The mRNA and protein expression of eIF3α and p27 were analyzed by real-time PCR and Western blot. Results Immunocytochemistry staining identification of vimentin was positive in the third generation of pulmonary fibroblasts .TGF-β1 could obviously promote the eIF3α expression and proliferation of pulmonary fibroblasts. EIF3a siRNA was transfected with lipofectamine into cells, which obviously inhibit eIF3α expression induced by TGF-β1 , reversed cell proliferation induced by TGF-β1 , and could obviously promote p27mRNA expression induced by TGF-β1 . Fluorofenidone (3, 10 mmol?L-1 )could obviously inhibit cell proliferation induced by TGF-β1 and reduce eIF3α expression of pulmonary fibroblasts promoted by TGF-β1 . The inhibition rate was 66.7% and 78.8% respectively. Fluorofenidone (3 mmol?L-1 ,10 mmol?L-1 ) could also obviously reverse p27 expression inhibited by TGF-β1 , the reverse rate was 75. 5% and 71. 3% respectively. Conclusion eIF3α/ p27 signal pathway involves in pulmonary fibroblasts proliferation induced by TGF-β1 .Fluorofenidone can inhibit pulmonary fibroblasts proliferation induced by TGF-β1 by inhibiting eIF3α expression, enhancing p27 expression and/ or increasing p27 directly.

Leucocoprinus birnbaumill (Corda)Sing.is a new recorded species in Sichuan province,which belongs to Basidiomycota.It is a poisonous and view and admire mushroom.Hyphae of Leucocoprinus birnbaumii (Corda) Sing.have not clamp connection,but can form sclerotium.This is a rare event of Agaricales.The morphological characteristics,physiological characteristics of mycelium and fruit\|body,and the aftificial cultivate technology of this mushroom are report in this paper.It is a typical medium\|hightemperature mushroom.Grass powder,cow dung,wheat bran and cultivated spent compost of edible fungi etc,conventional combination are cultural fruiting substratum;with bottle,bag,beds cultivation are all feasible,the biological efficiency of dry mushroom is about 5%～7 5%.

Objective To compare the effect of application of constant speed injection pump and manual injection on hysterosalpi-nography (HSG).Methods A total of 220 infertile patients were selected and classified into experimental group and control group. Then the patients were scheduled to undergo HSG.The experimental group was undergone constant speed pump injection and control group manual injection.Observe the effectiveness of the two methods on patients in two groups,respectively.Results The excellent rate of the images in experimental group (94.55%)was significantly higher than the control group (85.45%)and the risk of adverse reactions were significantly decreased in the former.There was statistical difference between the two groups.Conclusion Constant speed infusion pump is a convenient,safe and effective method in hysterosalpinography compared with manual injection,which is worthy of application in clinic.

OBJECTIVE: To investigate the effect of PNS on experimental atherosclerosis in rabbits, including the serum levels of TG, TC, LDL-C, level of MDA, activity of SOD and plaque area. METHODS: White Japanese rabbits were divided into normal control group, AS model group, low dose PNS group and high dose PNS group. Administration was for 12 consecutive weeks. The serum levels of TG, TC, LDL-C, MDA and activity of SOD were determined before experiment and at the end of the 12th week, respectively. RESULTS: The serum levels of TG, TC and LDL-C in AS model group were significantly higher than that in control group ( P

BACKGROUND:Posttraumatic osteomyelitis is bone infection after surgery of open fractures or other joint, which leads to more secretions in antrum or bone exposed wounds, long treatment cycle, delayed healing of fracture, even disunion. The key link in the treatment is complete debridement, adequate drainage, and the use of external fixators with low interference and far away from lesions. ＜br> OBJECTIVE:To observe the clinical efficacy of external fixator combined vacuum sealing drainage (VSD) in the treatment of post-traumatic osteomyelitis, and compared with conventional catheter drainage. ＜br> METHODS:Since June 2010 to June 2013, 21 patients of post-traumatic chronic osteomyelitis were included in this study and divided into VSD group (n=11) and conventional catheter drainage group (n=10). Al patients in VSD groups underwent debridement and VSD was used to fil dead space, then the wounds were sutured. Cases appeared nonhealing fractures underwent external fixation, 10 cases using external fixators and 1 case retained original external fixator. Al patients achieved the closed wounds at the second phase, 9 cases were directly sutured, and 2 cases received skin flap transplantation. Among them, 1 case received autologous bone graft at the third phase because of bone defects. In the conventional catheter drainage group, al patients underwent debridement and conventional catheter drainage to rinse the wounds after surgery, 3 cases failing to close the wounds changed to receive ordinary dressing and skin flap transplantation at the second phase. Cases appeared nonhealing fractures underwent external fixation. Local swel ing, pain and fever were observed after treatment. The time of total hospitalization stay and fracture healing was recorded. ＜br> RESULTS AND CONCLUSION:The VSD group quickly control ed preoperative symptoms such as swel ing and pain and fever than the conventional catheter group (P＜0.05). The length of hospital stay showed no significant difference between the two groups (P>0.05). Al patients were obtained through clinic service and fol owed up for 24-36 months. The healing time in the VSD group was shorter than that in conventional catheter group (P＜0.05). Until the end of fol ow-up, no patients in the VSD group appeared infection recurrence, and two cases in the conventional catheter group had recurrent infection. The results suggest that VSD combined with external fixator can repair post-traumatic chronic osteomyelitis due to rapid control of infection, easy care, shortened treatment time, and reduced rate of postoperative recurrence. In addition, external fixator can be used as the final fixation for post-traumatic chronic osteomyelitis.

Objective To investigate the clinical effect of standard large decompressive craniotomy in treatment of intracranial hematoma induced by severe traumatic brain injury (sTBI) and its influence on neuron-specific enolase (NSE) and inflammatory cytokines.Methods A total of 168 patients with sTBI-induced intracranial hematoma were assigned to standard large decompressive craniotomy (standard group) and traditional treatment (control group),with 84 patients per group.Glasgow outcome scale (GOS) and levels of serum NSE,TNF-α,IL-6 and IL-10 were compared between the two groups after surgery.Results GOS was better in standard group than in control group after surgery (P ＜0.05).Standard group presented significantly lower levels of serum NSE,TNF-α and IL-6 (P ＜ 0.05),significantly higher level of IL-10 (P ＜ 0.05) and a dropped ratio of IL-6 to IL-10 after surgery.However,there was no significant change in control group.Mean survival time of the patients in standard group was (33.11 ± 0.62) months.Two-year and three-year survival rate were significantly higher in standard group than in control group.Conclusion Standard large decompressive craniotomy cna enhance the clinical effects in patients with sTBI-induced intracranial hematoma,improve their neurologic deficits and daily living ability and decrease their body inflammatory stress level and injury.

Objective To investigate treatment and related prognostic factors of tongue carcinoma .Methods Clinical and patho-logical parameters of 1 171 cases of tongue carcinoma treated in Department of Oral and Maxillofacial Surgery ,School of Stomatolo-gy ,Harbin Medical University and Central South University from 2001 to 2007 were retrospective analyzed ,which included age , gender ,location ,pathological grade ,clinical TNM stages ,and treatment modality .Results After 3 to 5 year of follow-up ,survival rate of patients with tongue body carcinoma was obviously higher than that on the base of tongue .High clinical stage ,low cell dif-ferentiation degree of the postoperative patients had lower survival rate .The prognosis of patients with positive surgical margins and positive lymph node metastasis was poorer .The survival rate had no correlation with excision of mandible .When the tongue was re-stored with flaps ,the safety margins were increased to reduce recurrence .Conclusion Clinical TNM stages and surgical margin are independent prognostic factors for tongue cancer .Early diagnosis and treatment are vital to increase the survival rate of patients .

Objective To examine the expression of tight junction protein claudin-5 in blood-spinal cord barrier (BSCB) after spinal cord injury about rat. Methods One hundred-twenty adult male SD rats were randomly divided into blank group (60) and injured group (60). The animal model of spinal cord injury was established using modified Allen method. The expression of claudin-5 in BSCB was examined at 6 h, 12 h, 1 d, 3 d, 5 d and 7 d (five rats per time point). Western blot and RT_PCR were used to detect protein and mRNA expression levels of claudin-5, respectively. Results The success rate of spinal cord injury molding was 81.7%. In injured group, EB content increased gradually over time, reached the peak at the third day(0.9435 ± 0.0813)μg/g and then reduced gradually (P<0.05), EB content was signifi-cantly higher in injured group than in blank group. Claudin-5 mRNA expression in injured group reduced gradually over time and reached the lowest point at the third day(2.871 ± 0.527)and then increased gradually(P<0.05). Claudin-5 mRNA expression was significantly lower in injured group than in blank group(P<0.05). Claudin-5 protein expression in injured group reduced gradually over time, reached the lowest at the third day(0.072 ±0.008)and then increased gradually (P<0.05). Claudin- 5 protein expression was significantly lower in injured group than in blank group(P<0.05). Con-clusions The alteration of claudin-5 expression after SCI may lead to the permeability of BSCB, which may in turn con-tribute to the secondary spinal cord injury.

Aim To investigate the neurotoxicity and the changes of nitration in the striatum of rats treated with methamphetamine(MA).Methods The rats were randomly divided into MA group and control group.The stereotyped behavior and body temperature of the rats were recorded.The differences of nitric oxide, nitric oxide synthase(NOS)and nitroprotein were compared between the two groups.Results The score of stereotyped behavior and the body temperature of the rats in MA group were significantly higher than those in the control group(P ＜0.01);Compared with the control group, the content of NO and the activity of NOS of striatum in MA group were significantly increased(P ＜0.01), and nitration of protein was also upregulated(P ＜0.01).Conclusion The increases of NOS activity, NO level and nitroprotein may play an important role in MA-induced neurutoxity in rat striaturn.