Persistent pulmonary hvpertension of newborn(PPHN) is a life-threatening condition which occurs at a rate of 2/1 000-6/1 000.The etiology of PPHN is still unknown and its treatment remains a major challenge for neonatologist.Impaired pulmonary artery relaxation,reduced blood vessel density and pulmonary vascular remodeling are the main pathological changes in PPHN.The general managements include mechanical ventilation,sedation,surfactant and hemodynamic support.Inhaled nitric oxide is the main vasorelaxant used in the treatment of PPHN.Several other vasorelaxants have been studied such as phosphodiesterase inhibitors,prostacyclin,endothelin-1 inhibitors.Even with the introduction of several new therapeutic modalities there has been no significant change in survival rate.

Objective To investigate the effect on short-term continuous subcutaneous insulin infusion (CSII) treatment on beta-cell function,serum triglyceride(TG),C-reactive protein(CRP) and serum insulin like growth factor-1 (IGF-1) of newly diagnosed type 2 diabetes mellitus(T2DM) patients.Methods Thirty-two newly diagnosed T2DM patients with fasting plasma glucose(FPG) ＞ 11.1 mmol/L were treated with CSII for 2 weeks.FPG,2 h postprandial blood glucose (2hPG),fasting insulin (FINS),2 h postprandial insulin (PINS),fasting C peptide (FC-P),2 h postprandial C peptide (PC-P),glycosylated hemoglobin (HbA1 c),Homa-β,Homa-R,TG,CRP and IGF-1 were measured and compared before and after CSII.Results (1) Compared with that of before treatment,the levels of FPG,2hPG,HbA1c,PINS and PC-P in patients after treatment were dramatically improved(t =12.04,11.57,7.61,20.77,6.16; P ＜ 0.05),and Homa-β increased from (23.0 ±16.2) to (71.0±23.8)(t=9.46,P＜0.05),while Homa-IR significantly decreased from (6.8 ±2.1) to (3.9 ± 2.6) (t =4.81,P ＜ 0.05).(2) The levels of TG and CRP significantly decreased from (1.50 ± 0.32) mmol/L,(3.14±1.14) to (1.29±0.31) mmol/L,(1.95 ±0.52) mg/L(t =26.73,23.62;P ＜0.05).The levels of IGF-1 significantly increased from (341 ± 16) μg/L to (471 ± 17) μg/L (t =8.65,P ＜ 0.05).Conclusion The excellent glycemic control,improvement of lipid metabolism,anti-inflammatory and improvement of beta-cell function can be achieved with short-term CSII intensive therapy in newly diagnosed T2DM patients with severe hyperglycemia.

Chronic myeloid leukemia (CML) is one of myeloid malignancies,and there is no effective treatment method at present.Nowaday,many studies report that combined treatment and adoptive immunotherapy can rebuild immunological function of patients,it's an ideal method of cure CML.So searching optimal leukemia-associated antigens and researching the number of antigen specific T lymphocytes is the key to treat CML.

Asthma is a extensive airway limited disease caused by allergens or other factors and emerging under bronchial hyper-responsiveness,which is a common chronic respiratory disease.Airway inflammation and airway remodeling is the main feature of bronchial asthma,and airway remodeling is the main pathological basis causing the irreversible airflow obstruction and pulmonary function injury of asthma.The main pathological change of airway remodeling is airway smooth muscle layer thickening induced by airway smooth muscle cell (ASMC) proliferation,and the mast and increasing number of ASMC under the action of various cytokines is the main cause of smooth muscle layer thickening.The function of airway smooth muscle cells in the airway remodeling is described.

Objective To evaluate the efficiency of entecavir in chronic hepatitis B (CHB)patients.Meathods 60 cases of pa-tients treated with entecavir 0.5 mg/d.Observed the negative rate of HBV DNA and ALT after 3,6 and 12 months.Used immuno chemiluminescence to observe the HBeAg titer after medication.Results The negative rate of ALT and HBeAg, HBeAg conversion rate were 91.7%,86.7% and 16.7%,respectively.The titer of HBeAg was low at baseline,the patients would had higher HBeAg seroconversion,there were significant differences with other groups.The titer of HBeAg decreased≥90% patients was significantly higher in patients with < 90% on the 3 months.Conclusion The HBeAg titer could be predicted ETV for treatment of HBeAg positive CHB patients.

[Objective] To discuss the importance of causa morbi and early diagnosis and treatment on the prognosis and lapsing of hypoglycemia.[Method] Select 33 cases and make detailed enquiry and analysis to their causa morbi, occurrence time length and complications, supplement timely glucose injection, if necessary, administer glucocorticoid for treatment; observe the patients ’clinical symptoms relieve, and compare the usage of glucose and glucocorticoid, the in-hospital period and relative indexes of prognosis and lapsing.[Result] In the group, 28 cases are drug-induced hypoglycemia, occupying 84.85%;30 cases have the period less than 6h from occurrence to diagnosis, among which, 1 had cerebrovascular accident and consulted with neurology department, relieved with treatment, but with sequela of hemiplegia; 1 case had too long time of dizziness before coming to hospital(>24h), couldn ’t be better over treatment, the relatives gave up the treatment and went out of hospital; 1 was tested of islet celltumor and transferred to upper hospital, without recurring hypoglycemia being fol owed up.[Conclusion] Drug-induced hypoglycemia is the main reason for hypoglycemia, with much harm;early diagnosis, remov-ing causa morbi and active rescuing wil have good prognosis.

Objective To explore the effect of candesartan combined with levamlodipine besylate on hypertension and target organ protection.Methods One hundred and twenty-two patients with hypertension and proteinuria from February 2011 to July 2013 were divided into observation group (61 cases) and control group (61 cases) by random number table method.Patients in both groups were given oral candesartan 8 mg/d,and the observation group was treated with levamlodipine besylate 2.5 mg/d on this basis.Six months after treatment,the blood pressure levels,carotid intima-media thickness (IMT),interventricular septum thickness (IVST),left ventricular posterior wall thickness (LVPWT),left ventricular end diastolic diameter (LVEDD),left ventricular mass index (LVMI) and renal function between 2 groups were observed.Results After 6 months of treatment,the total effective rate in observation group was 86.9％ (53/61),and in control group was 72.1％ (44/61),there was significant difference (P ＜ 0.05).Systolic blood pressure and diastolic blood pressure levels in two groups after treatment were decreased,in observation group which were lower than those in control group,and there was significant difference (P ＜ 0.05).IVST,LVEDD,LVPWT,LVMI and carotid IMT in 2 groups after treatment was lower than that before treatment,in observation group which was obviously lower than that in control group [(8.5 ± 1.3) mm vs.(9.2 ± 1.2) mm,(39.6 ± 4.8)mm vs.(44.3 ± 3.9) mm,(8.4 ± 1.4) mm vs.(9.1 ± 1.3) mm,(89.4 ± 9.3) g/m2 vs.(97.3 ± 8.9) g/m2,(8.3 ± 0.3) mm vs.(8.7 ± 0.5) mm],and there were significant differences (P ＜ 0.05).Urea nitrogen between 2 groups before and after treatment had no significant difference(P ＞ 0.05).The serum creatinine,creatinine clearance rate,total 24 h urinary albumin and microalbuminuria in 2 groups was significandy improved compared with that before treatment,and in observation group after treatment was obviously improved compared with that in control group [(94.6 ±25.5) μmol/L vs.(118.5 ±38.4) μmol/L,(102 ±24) ml/min vs.(96 ± 18) ml/min,(1.05 ± 0.51) mg/24 h vs.(1.27 ± 0.22) mg/24 h,(155 ± 36) mg/L vs.(185 ± 41) mg/L],there were significant differences (P ＜ 0.05).Conclusions The effect of candesartan combined with levamlodipine besylate in treatment of hypertension and proteinuria is better than monotherapy.Combination therapy in reversing left ventricular,improving carotid IMT,protecting kidney function and reducing proteinuria has significant effect,and has no obvious adverse reactions.It is worthy of clinical application.

Danggui Injection and Vitamin B12 Injection were injected into acupoints Geshu (BL17), Pishu (BL 20), Zusanli (ST 36), Fenglong (ST 40) and Neiguan (PC 6) to treated 56 cases of hyperlipidemia. Meanwhile, Fenofibrate was adopted to treat another 45 cases as control. After2-month treatment, result showed similar effects in the two groups.

The failure of islet β cell is the core pathogenic mechanism of diabetes,which is also the key target for type 2 diabetic mellitus treatment.A large amount of basic and clinical researches have been confirmed that glucagon like peptide-1 and its analogue liraglutide have abilities to significantly improve islet β cell function besides the glucose-dependent lowering hyperglycemia effects.Furthermore,liraglutide is approved to improve β cell function,thus providing a new choice for treatment of type 2 diabetes.

Objective To study the applied value of invasive arterial blood pressure monitoring in the diagnose and treatment of coronary artery intervention. Methods The dynamic changes of ECG, the non-invasive blood pressure, the invasive arterial blood pressure monitoring and the clinical performance were ob-served in 163 patients in the process of coronary artery intervention (CAI). Then the invasive arterial blood pressure change and the non-invasive blood pressure change were compared. Results The change rates of invasive arterial blood pressure were higher than that of non-invasive arterial blood pressure. The difference of both changes had statistical significance. The invasive blood pressure decreased and wave forms changed in 26 cases. These reflected sensitively the patients' condition and the issues in the process of CAI. Conclusions To strengthen the invasive arterial blood pressure monitoring can improve the success rate of CAI more effec-tively and reduce the complication.