OBJECTIVE： To discuss about the key and entrance points of the development strategy of Chinese materia medica in the new century.METHODS： Some important items,such as difficult problems which Chinese materia medica facing to at present,new way to research into its effective substance bases,innovation on the theory and “ molecular targets” of Chinese materia medica,were analyzed.RESULTS ＆ CONCLUSION： Chinese materia medica industry would become a most important pharmaceutical industry in China and really walk up to the whole world if traditional Chinese herb medicine supplies“ international language” for its exchange,qualitative and quantitative standards on judging the essence of its prescriptions and builds up its“ earmark” of Chinese materia medica industry.Based on the train of thought of this paper,suggestions how to promote the development of Chinese materia medica in the new century are put forward.

Accurate alignment of knee implants is essential for the success of total knee replacemem.Recently,computer-aided navigation systems have become available,and the results showed more accurate and consistent installation of knee implants.The technology has evolved rapidly with widespread and continue to be developed.The current computer environmem and graphical user interface will become more user friendly and thus gain more widespread acceptance among experienced surgeons With this technology growing and systems evolving,computerized navigation will become the standard of care in knee reconstructive surgery.The key of future studies is to evove less invasive tracker anchoring systems,electronic balancers and computerized saws.A hybrid technique between image-based and image-free methods may develop to produce image-enhanced navigation.It should be an intermediary step between current technology and a fully robotic system.

Since the first session of Chinese anti-inflammatory immunopharmacological society in 1982,many breakthroughs of immunological research have been made in western countries,such as identification of pathogens by natural immune cells,regulation of immune response,lymphocyte differ?entiation and development. At the same time,much progress has been achieved in anti-inflammatory and immunological research in China,especially in the field of basic scientific issues in immunology, frontier and hot topics,immunological mechanisms of major diseases and related drugs. The course of development of Chinese anti- inflammatory immunopharmacological research has experiences various stages,such as ″follow ″ in the past to ″work together ″ and to ″lead ″ in some research areas in today′s international immunology.

The cells sense direction is closely related with the proteins that contain PH (pleckstrin homology) domain. PH domain has been found in about 60 proteins, many of which could activate the sequent events of signal transduction via combining with the related binding sites on the surface of chematactic cel ls. The characteristics of this combining are: a.rapid and transient; b.It is only related to the concentration gradient of surroundings outside cells, whi ch is the base of spatial model; c.The distribution of the binding sites on th e cell membrane changes when the researchers altered the position of the chemoat tractant. This is the base of temporal model. To deeply investigate the effects of all kinds of proteins which contain PH domain on cells sense of direction will greatly promote the research of this field, and hence, has great theoretica l significance.

AIM:To study of effects of Panax notoginsengnoside(PnGL) on neutrophil(Neu) functions in carrageenin(Car) induced subcutaneous air-vesicle synovitis in rats and its mechanisms METHODS:Rat air-vesicle synovitis model was established with sc Car(25mg/kg) Neu count,the phagocytosis of Neu,the realease of O2 ?- from Neu and the level of Neu-i were measured with test tube diluting method,staphylococcus aureus phagocytosis method,cytochrome C reduction method and Fura-2 fluorescence technique respectively RESULTS:At 12h after Car used,Neu count of exudates elevated,phagocytosis rate and index of Neu and the release of O2 ?- from Neu increased obviously,suggesting that Neu was in an activated state Mea_nwhile,the level of Neu-i elevated significantly PnGL 60～240mg/kg ip,30min before and 6h after Car sc,reduced Neu count of exudates,inhibited phagocytosis functions and the increase of i level of Neu in a dose-dependent manner There was a significant correlation between the reduction of Neu-i and the inhibition of Neu phagocytosis functions and the release of O2 ?- from Neu Dexamethason had similar effects on Neu functions to PnGL,but with no effect on the level of Neu-i CONCLUSION:The reduction of Neu-i level and the subsequent inhibition of Neu functions is one of the important anti-inflammatory mechanisms of PnGL,which is different from that of Dex

Nonsteroidal anti inflammatory drugs (NSAIDs) are among the most commonly used drugs in the world, but their use is limited because of their propensity to cause many severe adverse reactions. Cyclooxygenase 2 (COX 2) not only take part in inflammatory reaction, but also exert various physiological functions. Selective COX 2 inhibitors still have a series of adverse reactions. COX 3, a new COX cloned and expressed recently, can be inhibited selectively by acetaminophen and do not relate to inflammation. A hypothesis about COX family is assumed and seven isoforms are supposed in this article.

Objective To investigate skin rash characteristics in patients with dengue fever, and to explore factors associated with the occurrence of skin rashes in dengue fever. Methods Skin rashes were observed in 127 hospitalized patients with dengue fever. Clinical and laboratory data were collected from these patients. Statistical analysis was carried out by using a two-sample t-test and chi-square test with the SPSS16.0 software. Results Out of the 127 inpatients, 88 (69.29%)had skin rashes, which usually developed within 5-7 (average, 5.06 ± 2.50)days after fever onset. Among the 88 patients, 37(42.05%)developed skin rashes during fever, while 51(57.95%)after the drop of body temperature. Moreover, 48 (54.55%) patients complained of itching, while 39 (44.32%) never felt itch, and 1 complained of a tingling sensation all over the body. The type of skin rashes was various. Congestive erythema and macules were observed on both palms in 26 (29.55%)patients, and might be the characteristic skin presentation of dengue fever. Skin rashes usually began in both lower extremities, whereas the order of their subsidence was unclear. There was a significant difference in blood platelet (PLT) count as well as alanine transaminase and aspartate transaminase levels between patients with skin rashes and those without(all P=0.00). However, no significant difference was observed in the count of leukocytes, neutrophils or PLT, or the levels of alanine transaminase or aspartate transaminase between patients with hemorrhagic rashes and those with nonhemorrhagic rashes or between patients with itching and those without (all P>0.05). Conclusions Dengue fever has various skin rashes, and congestive erythema and macules on both palms may be the distinctive presentation of dengue fever.

Objective To explore neuroprotective effect of escitalopram oxalate on cerebral ischemia reperfusion in injury rats and its possible mechanism,thus provide experimental evidence for the use of the drug in the clinical treatment of ischemic cerebrovascular disease.Methods 160 male rats of SD were selected and randomly divided into three groups:sham operation group(Sham group),negative control group(NS group),intervention group(CIT group,for 14 days and 21 days).Focal middle cerebral artery occlusion model(MCAO)of rats were constructed.Neurological deficit of rats in each group were evaluated by modified neurological severity score(mNSS)scale.Microvessel diameter,density,and total area of each group angiogenesis in cerebral ischemic area were observed by Laser cofocal technology after 14 days and 21 days at different time points;the plasma concentrations of VEGF were detected by ELASA,and expression levels of VEGF were detected by immunohistochemical assay and Western blot in each group,to explore its possible mechanism of molecular biology.Results After modeling,neurological deficit of intervention group for 14 days and 21 days were improved significantly.The indicators were as follows:scores of mNSS in NS group(6.57 ±1.13)was significantly higher than(4.39 ±0.92)in intervention group for 14 days(P<0.05);scores of mNSS in intervention group(3.23 ±0.55)for 21 days was significantly lower than(4.14 ±0.74)in intervention group for 14 days(P<0.01).Confocal 3D imaging results after 14 days showed:microvascular total area was significantly lager which illustrated that the effect of drug intervention for 14 days started to work over time.Plasma concentrations and expression levels of VEGF in intervention group for 14 days and 21 days were higher than those in Sham group,NS group.VEGF protein expression of cerebral ischemia tissues in intervention group for 21 days were higher than that of 14 days(P<0.01).Conclusion Escitalopram oxalate could significantly reduce cerebral ischemia and reperfusion in rats nerve injury and improve neurological function,which has a neuroprotective effect,the effect of drug intervention gradually increases over time,and the possible mechanism of which is related to angiogenesis mediated by VEGF.

Objective To explore the influence of pomegranate juice on cognitive behavior of chroniccerebral hypoperfusion in rats and its mechanism.Methods 48 healthy male Wistar rats were selected and randomly divided into 3 groups, sham operation group (SO), permanent bilateral persistence commancarotid artery occlusion or two-vesselocclusion (2VO) group, pomegranate juice group.Chronic cerebral hypoperfusion model was prepared in rats by permanent bilateral common carotid artery ligation.Given the intervention after operation 1 weeks, before the 2 groups were given by sugar water intragastric ( every 100 mL syrup containing 25 g,sucrose 2 g, fructose glucose 2 g) with pomegranate juice, pomegranate juice group ( polyphenol content of 2.4-4.8 mg/mL) were given by gavage,2 mL/d, a total of 7 weeks.8 th weeks after surgery, cognitive behavior ability of 3 groups was tested by space exploration target quadrant swimming time after an interval of 1day;CA1 region of 8-hydroxy deoxyguanosine (8-OHdG), 4-hydroxyl nonenal ( 4-HNE ) expression of 3 groups in cortex and hippocampus detected by immunohistochemical method; observed whether it could improve the chronic cerebral hypoperfusion in rats learning, memory ability, or had antioxidative stress injury.Results On the 4th day and 5th day, the time of rats in SO to find the platform and the shortest time, group 2VO for the longest time, compared with statistical significance between the three groups (P<0.01); the 3 groups of rats in the target quadrant emergence time in sham operation group, the longest (10.05+1.688)s, 2VO group has the shortest time (6.343 ±1.220) s, and there was statistical significance (P<0.05).The integral optical density of positive cells in 3 groups of rats cortex and hippocampal CA1 region of 4HNE, the value of the 8-OHdG IOD is the lowest in the sham operation group, 2VO group was the highest, with statistical significance.Pomegranate juice intervention, cortex of rats and hippocampal CA1 region of 8-OHdG, 4-HNE decreased significantly ( P <0.05).Conclusion Chronic cerebral hypoperfusion may cause oxidative stress injury, pomegranate juice can improve the low irrigation learning and memory ability of rats with chronic cerebral, decrease 8-OHdG and 4-HNE expression may play a protective function through antioxidative stress pathway.