5cm had the highet positive rate, while tumor with diameter

Combined operation and irradiation therapy has been performed on 22 patients with primary liver cancer in our hospital.All these patients were mal s.Age ranged from 15-49 years old with an average of 44.5. Histological dingnoais were. hepatocellular carcinoma in 21 cases and cholangiocarcinoma in 1 case. Seventeen (77.3%) patients were associated with liver cirrhosis, nineteen(86.4%)patients had positive AFP by counter- immunoelectrophoretic method.Clinically, 6 patients were stage I, 11 patients were stage II and 5 patients were stage III. The operative procedures consisted of liver resection in 17 patients (local resection 5, right hemihepatectomy 5, left lateral lobectomy 2, left hemihepatectomy 2, total right hepatectomy 2,and left medial lobectomy 1)and palliative operation in other 5 patients (hepatic artery ligation or embolization in 4 patients and hepatic artery infusion in 1 patient) .Radiotherapy comprised local or whole-liver irradiation with 60Co.Up to May 1983, 19 patients were followed-up, all of them survived more than 6 months.The 1, 2, 3, 4 year survival rates after treatment were 78.9%, 66.7%, 64.3% and 44.4% respectively with a median survival duration of 36 months.Data showed that the encouraging results of this method which were better than liver resection and radiotherapy or any other cmbined methods at present.

Objective To investigate the influence and clinical effect of assisted three-dimensional conformal radiation therapy (3D-CRT ) on obstructive jaundice caused by malignant tumors .Methods 90 cases with obstructive jaundice caused by malignant tumors of liver ,gallbladder and pancreases were randomly divided into observation group and control group ,45 cases in each group . Patients in control group were given comprehensive measures ,including tumors exairesis ,assisted chemotherapy and stereotactic ra-diotherapy ,and patients in observation group were given 3D-CRT based on tumors exairesis and assisted chemotherapy .Clinical effect ,serum bilirubin were observed 2 months after treatment ,adverse reactions were recorded during the treatment ,and survival rate in 2-year follow up in two groups were observed .Results Total effective rate in observation group and control group were 75 .56% and 57 .78% (P<0 .05) ,respectively ,and adverse reactions rate were 37 .78% and 57 .78% (P<0 .05) ,respectively .After treatment ,serum total bilirubin and direct bilirubin in two groups were all lower than those before treatment (P<0 .05) .Serum total bilirubin and direct bilirubin in observation group after treatment were (26 .3 ± 6 .5)μmol/L and(18 .9 ± 3 .3)μmol/L ,and were (39 .3 ± 9 .5)μmol/L and(26 .9 ± 6 .7)μmol/L in control group ,and there were statistical difference between two groups (P<0 .05) . Observation group′s survival rate was higher than that of control group (P<0 .05) .Conclusion It has good clinical effect of trea-ting obstructive jaundice caused by malignant tumors by 3D-CRT ,and it prolongs the survival time ,decreases serum bilirubin and improves jaundice symptom ,so it has some guiding significance to its treatment .

Objective: To assess the clinical application of transabdominal ultrasonography for the diagnosis of achalasia. Methods: The subjects were divided into 3 groups, with 8 patients suffered from achalasia as patient group, 10 normal individuals and 8 patients with cardiac carcinoma as controls. All subjects received water provocative test to study the lower esophagus and cardia. Sonographic findings were compared between the 3 groups. After treatment by administration of drugs, pneumatic balloon dilation, or intrasphincteric injection of botulinum toxin, the 8 patients were re-examined by TAUS for longitudinal comparison. Results: (1)The normal subjects had no dilation of cardia and lower esophagus before drinking water, but their cardias opened rapidly with the drinking action. (2)All the patients of achalasia were found to have dilation of lower esophagus before taking water. During 10 min continuous TAUS scanning, 5 of 8 demonstrated absence of relaxation of cardias and absence of water from the esophagus into the stomach. The other 3 showed slight and intermittent relaxation of cardias with water flow into the stomach. Thirty minutes after water provocative test, all the 8 patients had water retardation within the lower esophagus. (3)The patients of cardiac carcinoma had an ultrasound feature of cardiac ring enlargement and thickening of cardiac wall without dilation of lower esophagus before water provocative test. Water passed the cardia more slowly. Conclusion: TAUS is a readily accepted examination. It can quickly give reliable diagnostic clues to achalasia. TAUS can also be used for making assessment of therapeutic effect on achalasia.

Objective To analyse clinical and imaging characteristics of congenital airway abnormalities,and to guide the clinical diagnosis and reduce rate of missed diagnosis and misdiagnosis.Methods Clinical and imaging characteristics in children who had been diagnosed congenital airway abnormalities in Shengjing Hospital of China Medical University from 2005 to 2011 were reviewed and analysed retrospectively.Results Of 23 children,12 cases were male,11 cases were female,the age was from 1 month to 10 years,the average age was 32 months.The disease course was from 3 days to 3 months,an average was 18 days.Clinical manifestations were repeated coughing,wheezing,dyspnea and recurrent respiratory infection.Through the spiral CT and three dimensional reconstruction,enhanced CT examination,6 cases of tracheobronchial stenosis,3 cases of trachea and bronchus,1 case of dysplasia of the left pulmonary artery and vein,3 cases of pulmonary sequestration,4 cases of pulmonary cyst and 6 cases of pulmonary hypoplasia were found.Conclusion Missed diagnosis and misdiagnosis of congenital airway malformations often occurs.When the following features were observed,congenital airway malformations may be taken into account:persistent cough; repeated or persistent wheezing,treated ineffectively with anti-inflammatory agent and bronchodilators ; recurrent or persistent stridor; repeated pulmonary infections,dyspnea; repeated or persistent pneumonia in the same part and unexplainedly mediastinal shift in X ray chest radiography.Spiral CT three dimensional reconstruction and enhancement is of great value in diagnosis of airway malformation.

Interleukin-15 is a multffunctional cytokine,which are the activating and induction factors of the T-cell,B-cell,NK-cell and lymphokine-activated killer cell (LAK).It can stimulate hematopoietic stem cell proliferation and differentiation,enhance immunity and has anti-tumor effects.IL-15 and IL-2 have similar structures and functions,but many activating effects of IL-15 are stronger than the other's.IL-15 is closely related to tumor development,and has broad application prospects in biological therapy of cancer.

Aim To observe the effects of chrysophanol on memory impairment and synaptic transmission in the dentate gyrus of hippocampus in AD rats induced by ?-amyloid peptide(A?25-35).Methods AD rat model was established by injection of A?25-35 into cerebral ventricles with cerebral three-dimensional positioning.Chrysophanol of different doses(0.350,0.070 and 0.014 mg?kg-1 ip) were administered continuously.Through the methods of morris water maze test and electrophysiological test,the effects of chrysophanol on memory impairment and long-term potentiation(LTP) induced by high-frequency stimulation(HFS) in AD rats were observed.Results Chrysophanol(0.350,0.070,0.014 mg?kg-1,ip) does-dependently shortened the time of seeking platform and significantly improved the LTP induced by HFS in the DG of hippocampus in AD rats.Conclusion Chrysophanol can improve memory impairment and possess enhancement property on the LTP induced by HFS in AD rats induced by A?25-35.

Objective:To construct Cyanovirin-N(CV-N) expression plasmid pET-CVN and express the protein in E.coli, and to prepare specific antibody against Cyanovirin-N.Methods:In this study, the encoding sequence of Cyanovirin-N was obtained by PCR. The expression plasmid pET-CVN was constructed by inserting the encoding sequence of CV-N into pET-His containing T7 promoter and then BL21(DE3)strain was transformed. Expression of CV-N was induced by IPTG at 30℃ for 8 h. Cyanovirin-N was refolded by using dialysis method. The target protein was purified through Ni2+-NTA Agarose Fast Flow. The purified Cyanovirin-N was used as an antigen to immunize rats,and the polycolonal antibody to Cyanovirin-N was obtained.Results:Cyanovirin-N was cloned and expressed in E.coli successfully. SDS-PAGE analysis showed that the Cyanovirin-N was expressed in the form of insoluble inclusion body,and the expression amount of Cyanovirin-N was about 42.08% of total protein. With ELISA detection the titer of antibody was more than 1∶8 000, Western blot analysis showed that the antibody can bind with Cyanovirin-N specifically.Conclusion:Cyanovirin-N expression plasmid pET-CVN has been constructed, Cyanovirin-N with bioactivity has been obtained. The prepared antibody against Cyanovirin-N has well specificity and reactivity and can be used to detect the expression products by recombinant strains of E.coli.The work lays a foundation further study of its functions.

Objective: To explore the relationships between Reinforcement sensitivity, Internet service hobby and Pathological internet use. Methods: 197 junior middle school students were administrated to complete Behavioral Inhibition System and Behavioral Activation Scale(Chinese edition), Adolescents Internet Service Hobby Questionnaire, and Adolescents Pathological Internet Use Scale. Results: ①For Internet service hobby and PIUs, the Social hobby and the Entertainment hobby wre somewhat correlated to PIUs positively; however, the Information hobby and the Bargains hobby were somewhat correlated to PIUs negatively, but not significantly; ②For Reinforcement sensitivity and PIUs, Path analysis suggests, high BAS individuals were easily involved in the PIU, but high BIS individuals were not. Conclusion: Reinforcement sensitivity is a significantly predictor of PIUs, but the Internet service hobby is not.

Objective:To investigate learning burnout of undergraduates and its relationship with health-related hardiness.Methods:408 undergraduates were investigated with Undergraduate Learning Burnout Scale and Health-Related Hardiness Scale.Results:Boys scores were significantly higher than those of girls in behavior inadequacy, reduced personal accomplishment and general burnout;significant differences were found between grades in emotion turndown and majors in emotion turndown, reduced personal accomplishment and general burnout.Health-related hardiness has a sig-nificant negative correlation with learning burnout.The results of regression analysis found that control and commitment were the effective precursors.Conclusion:Health-related hardiness has a significant influence on undergraduates' learning burnout.