Many genetic events have been described to be involved in the development of tumor,but a lot of recent researches proved that epigenic events also played an important role in it.DNA methylation is one of the common modification of epigenic events,the binding of methyl-CpG binding domain proteins has been shown to lead to transcriptional repression of many tumor repressor genes.

OBJECTIVE:To establish a method for the contents determination of gallic acid,naringin,hesperidin,neohesperi-din and baicalin in Qinghou liyan granule. METHODS:UPLC was performed on the column of ACQUITY UPLC BEH C18 with mobile phase of acetonitrile- 0.1% phosphoric acid(gradient elution)at a flow rate of 0.40 ml/min,detection wavelength was 280 nm,column temperature was 30 ℃,and injection volume was 1 μl. RESULTS:The linear range was 0.048-0.480 μg for gallic ac-id(r=0.999 3),0.012-0.120 μg for naringin(r=0.999 9),0.016-0.160 μg for hesperidin(r=0.999 8),0.022-0.220 μg for neo-hesperidin(r=0.999 9)and 0.072-0.720 μg for baicalin(r=0.999 2);limit of quantitation was 2.4,1.6,1.8,1.8,1.6 ng,limit of detection was 7.5,5.0,5.6,5.6,5.0 ng;RSDs of precision,stability and reproducibility tests were no lower than 3.0%;recover-ies were 96.64%-102.02%(RSD=2.00%,n=6),95.86%-102.56%(RSD=2.86%,n=6),97.24%-102.54%(RSD=2.10%,n=6),97.44%-102.60%(RSD=2.40%,n=6)and 96.91%-103.13%(RSD=2.62%,n=6). CONCLUSIONS:The method is rapid and accurate,and can be used for the simultaneous determination of gallic acid,naringin,hesperidin,neohesperidin and baicalin in Qinghou liyan granule.

Objective To evaluate the effects of extractive from Gastrodiae Rhizoma on acquisition, consolidation and retrieval of learning-memory function in mice;To provide some reference for clinical research and development of new drugs.Methods Male Kunming mice were randomly divided into control group, model group, positive control group and Gastrodia extractive group. Positive control group and Gastrodia extractive group were given gavage by using relevant medicine 0.2 mL/10 g, and the control group and model group were given gavage with the same amount of distilled water for 16 days. After receiving gavage for continuous 11 days, memory acquisition barrier model was induced by scopolamine;memory consolidation barrier model was induced by chloromycetin;memory retrieval barrier model was induced by EtOH. The learning-memory function was reviewed by escape latency and spatial search distance. The quadrant and distance search time percentage was detected through directional navigation test and spatial probe test in Morris water maze.Results Extractive from Gastrodia Rhizoma shortened the time for acquisition, consolidation and retrieval of learning memory about escape latency and spatial search distance (P<0.05,P<0.01), and the quadrant and distance search time percentage were prolonged (P<0.01).Conclusion Extractive from Gastrodia Rhizoa can effectively improve the acquisition, consolidation and retrieval of learning-memory function in mice.

Clinical data of 61 cases of imported malaria were analyzed retrospectively.Patients with malaria were divided into three groups,asymptomatic tertian (vivax) malaria,symptomatic tertian malaria and pernicious (falciparum) malaria.Only mild hepatic damage occurred in some patients with asymptomatic tertian malaria,compared with other groups Symptomatic tertian malaria and pernicious malaria were misdiagnosed in 6 of 20 and three of six,respectively before hospitalization,and 16 of 20 and four of six patients complicated with thrombocytopenia,respectively,and both of them had increased serum level of C-reactive protein (CRP).Platelet count negatively correlated with their serum level of CRP significantly in patients with symptomatic tertian malaria (r =-0.555,P ＜ 0.05).Routine anti-malaria therapy was used in imported malaria,blackwater fever occurred in two patients and acute renal failure occurred in one with falciparum malaria.It is suggested plasmodium exam ination in peripheral blood should be performed in all persons returned from countries prevalent with malaria,thrombocytopenia is an indicator of acute malaria,and more severe complications usually tend to occur in falciparum malaria.

Objective To investigate the correlation between heat-shock protein 70-2 (HSP70-2) gene+1267A/G polymorphism and coronary heart disease (CHD) in Han Chinese population. Methods Using the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) , the polymorphism and genotype and allele distribution of HSP70-2 gene + 1267A/G in 185 CHD patients and 149 controls were analyzed. Results The HSP70-2 gene + 1267A/G polymorphism was found in this study population.The distribution of HSP70-2 genotypes was in Hardy-Weinberg equilibrium. The frequency of G allele in CHD group was significantly higher than that in control (61.89% vs51.68%, P ＜ 0. 01). After multiple logistic regression analysis, HSP70-2 gene (GG + AG) genotype was an independent risk factor of coronary heart disease. Conclusion HSP70-2 gene + 1267A/G polymorphism was associated with CHD risk of Han Chinese population, the G allele might serve as a genetic risk factor of coronary heart disease.

Objective To determine the pharmacokinetic parameters of P-methoxybenzyl alcohol from Gastrodiae Rhizoma in plasma of rats by HPLC. Methods Gavage and intravenous injection were employed for administration. HPLC was used to determine the concentrations of P-methoxybenzyl alcohol from Gastrodiae Rhizoma in plasma of rats in different time points. The pharmacokinetic parameters were computed by DAS3.0. Results The linear range of P-methoxybenzyl alcohol in plasma was 0.63-321.17 μg/mL, r 2=0.994 5. Intra-day accuracy, inter-day accuracy, absolute recovery and stability were in specified range. Conclusion The method is simple and accurate for the determination of the pharmacokinetic parameters of P-methoxybenzyl alcohol from Gastrodiae Rhizoma in plasma of rats.

Aim To investigate the effect of indirubin derivative PHⅡ-7 and TRAIL on proliferation in breast cancer cell MCF-7 and its MDR counterpart MCF-7/ADR and the mechanism.Methods Growth inhibition rate was examined respectively by MTT assay under treatment with TRAIL or PHⅡ-7 or in combination. Cell apoptosis and ROS production were examined by flow cytometry.The change of TRAIL receptors(DR4/DR5 )in mRNA was analysed by realtime PCR.Re-sults IC50 of PHⅡ-7 on MCF-7 and MCF-7/ADR was (4.49 ±1.55 ),(3.44 ±0.90 )μmol · L-1 respec-tively;MDA-MB-231 was TRAIL sensitive cell line, and apparently TRAIL induced apoptosis in MDA-MB-23 1 .Low concentration of PHⅡ-7 in combination with TRAIL could augment TRAIL-induced cytotoxic effect including apoptosis while TRAIL or PHⅡ-7 treatment alone had limited cytotoxity to those cells.Besides, PHⅡ-7 at this concentration had little toxicity to hu-man peripheral blood mononuclear cells even if in com-bination with TRAIL.PHⅡ-7 generated ROS produc-tion inside MCF-7 and MCF-7/ADR cells and up-regu-lated DR4/DR5 expression concentration dependently. Once upon ROS scavenger NAC involved,the effect of TRAIL receptors up-regualtion by expression was abro-gated.Conclusions PHⅡ-7 at low concentration could improve the sensitivities of breast cancer cell MCF-7 and MCF-7/ADR to TRAIL,the mechanism of which may be the ability of ROS production by PHⅡ-7 help up-regulated TRAIL receptor DR4,DR5 .Our re-search set a solid foundation for PHⅡ-7 in combination with TRAIL in future clinical application.

Objective To conduct comparative study on the analgesic and anti-inflammatory effects as well as the acute toxicity of yunaconitine and 8-deacetyl-yunaconitine isolated from the processed products of Aconiti Knsnezoffii Radix.Methods The methods of hot plate test and writhing test were used to evaluate the analgesic effect. Anti-inflammation action was observed by the models of auricle swelling caused by dimethylbenzene. LD50 was determined by the method of Bliss.Results Yunaconitine and 8-deacetyl-yunaconitine have analgesia effect on the pain caused by hot-plate, but there were no statistically significant difference. The pain caused by acetic acid had obvious analgesic action. High and low dose of yunaconitine could significantly reduce the number of mice body torsion and extend the incubation period of pain in mice. The effect of 8-deacetyl-yunaconitine was remarkable only in the high dose. Compared with solvent group, there were little differences in inhibiting effect of auricle swelling caused by dimethylbenzene, and anti-inflammatory action was not exact. The poisonousness of yunaconitine was nearly 20 times of 8-deacetyl-yunaconitine.Conclusion Yunaconitine and 8-deacetyl-yunaconitine may be the analgesic medicine for peripheral analgesic effect. The poisonousness of 8-deacetyl-yunaconitine is less than yunaconitine, the effect is remarkable to the pain caused by acetic acid, and the security is high.

Objective To investigate the effects of azithromycin on airway remodeling and the expression of transforming growth factorβ1 (TGF-β1) in asthmatic mice.Methods BALB/c male mice were random divided into 3 groups:control group(A),asthma group (B),and azithromycin treated group (C),with 10 mice in each group.Bronchoalveolar lavage fluid was collected to count the number of white blood cells and eosinophilic granulocytes EOS.The morphological parameters of the bronchi were measured by computer image analysis and the pathologic changes of the bronchi and lung tissue were observed by HE staining.The expressions of TGF-β1 were detected by immunohistochemistry.Results The number of EOS in B group was significantly higher than that in control group(8.12 ±0.54 vs 0.70 ±0.40;8.12 ±0.54 vs 0.87 ±0.25,P ＜0.01).WAt and WAi and WAm in group C was significantly lower than that in group B (10.15 ±0.95 vs 15.36 ±0.85,4.16 ±0.32 vs 10.64 ± 1.03,3.77 ±0.15 vs 7.97 ±0.17,P ＜0.01)but higher than that in group A.The expression of TGF-β1 in group C was significantly lower than that in group B but higher than that in group A.TGF-β1 expression of lung tissue in C group was significantly correlated with EOS,(r =0.840,P ＜0.01) and WAt(r =0.735,P ＜0.01) and WAm (r =0.870,P ＜0.01).Conclusion Azithromycin inhibited airway remodeling in asthmatic mice,which might possibly be achieved through inhibiting the expression of TGF-β1.

Objective:The aim of this study was to observe the clinical effects of myopic foveoschisis (MF) via triamcinolone (TA) assisted fovea-sparing internal limiting membrane peeling (FSILMP).Methods:This study was prospective research, including 41 cases (44 eyes) of patients diagnosed with MF in Changsha Aier Eye Hospital from November 2018 to June 2020. All patients underwent combined TA assisted FSILMP and 25 G pars plana vitrectomy (PPV). The posterior vitreous cortex, epiretinal membrane and internal limiting membrane (ILM) in the macular area were labeled by TA particles. The corrected visual acuity, central retinal thickness (CRT), post-operative healing of myopic foveoschisis and the incidence of macular hole were observed. Facilitating statistics, in this study, decimal visual acuity was converted to logMAR visual acuity through the formula logMAR=lg(1/decimal visual acuity).Results:Three cases underwent binocular surgery and 38 cases underwent monocular surgery. The average age of the patients was (56.16±11.00)years old ranging from 30-73 year olds; the average axial length of the patients was (30.50±1.96)mm which was ranging from 26.19-34.52 mm. The corrected visual acuity was 0.1-3(1.65±0.67) and the CRT was 126-1 100(473.47±195.96)mm. The patients were followed up for 1-31(13.89±8.32)months. A total of 38 cases (41 eyes) were followed up and 3 cases were lost. Reduction of MF in 41 eyes: 5 eyes (12.2%) were not healed, 12 eyes (29.3%) were improved, 8 eyes (19.5%) were near healed and 16 eyes (39.0%) were healed. The incidence of macular hole was 4.9% (2 eyes). The postoperative corrected visual acuity was 1.00±0.62, which was significantly higher than the preoperative corrected visual acuity [(1.65±0.67), t=8.23, P<0.01]. The postoperative CRT was (295.88±167.55)μm, which was significantly lower than that before operation (473.47±195.96)μm( t=7.82, P<0.01). Conclusions:TA can better mark the vitreous cortex, epiretinal membrane and inner limiting membrane of high myopia MF, and avoid the retinal toxicity caused by repeated indocyanine green (ICG) staining. At the same time, the effect of TA assisted FSILMP is no less than that of ICG assisted FSILMP in postoperative visual acuity recovery, CRT and the incidence of postoperative macular hole.