0.05).Conclusion:In the progress of benign prostatic hyperplasia,the expression of VEGF and angiogenesis are significantly developed.VEGF palys a necessary role in pathogenesis and development of BPH,but Flt-1 is a less important factor.

ObjectiveTo explore the clinical-pathway-based teaching effect in teaching urological postgraduate students in department of urology.Methods36 students were divided to two groups:18 students are the clinical pathway based teaching group, and the other 18 students are traditional teaching group.Examination was used to compare the effect of each teaching method after 6 month.The examination including history collection,physical examination and case file writing,clinical case analysis,clinical operational skills test and basic theory.ResultsThe record of history collection,physical examination and case file writing and basic theory test in clinical pathway based teaching group is not different between the two groups,but the record of clinical case analysis and clinical operational skills test in clinical pathway group is better than the traditional teaching group.ConclusionClinical pathway based teaching can improve the teaching effect of clinical case analysis and clinical operational skills in urological postgraduate students,and can improve the strengthen of clinical thinking and working ability.

Objective To study the expression of cytokeratin(CK)20 in exfoliated urothelial cells and to evaluate its clinical significance. Methods The expression of CK20 in exfoliated urothelial cells in 40 patients with bladder cancer and 20 subjects with normal urothelium was observed with immunohistochemical methods. Results The positive staining of CK20 in patients with bladder cancer was 52.5%, the expression of CK20 was negative in all controls, the positive rate of expression of CK20 combining with HE staining in patients with bladder cancer was 90%. The expression of CK20 was significantly higher in recurrent cases(80%) than in primary cases(43.3%). CK20 was not related to the tumor grade or stage. Conclusions The results suggested that the method had a higher specificity and therefore CK20 would be a good diagnostic marker.

To improve the quality of full English teaching of urology for foreign students,our experiences are: choose the excellent teaching staff,choose and prepare teaching material carefully,make CAI courseware accurately and popularly,enrich teaching component,pay attention to the background of the foreign students,and apply new teaching model with communications.

Medical clinical education is really the beginning of clinical work,and it should be reformed accordingly based on actual changes in health care.This article discusses that the clinical medical education should be reformed to face serious medical problems.

Objective To investigate the effects of cyclopamine on the proliferation ,apoptosis and the expression of PSAmRNA in prostate cancer LNCaP cells .Methods LNCaP cells were interfered with different concentrations of cyclopamine (1 ,5 ,10 ,15μmol/L) at the different timepoints(24 ,48 ,72 h) .The poliferation inhibition was measured by the MTT assay ;the apoptotic mor-phological changes were observed by the Hoechst33258 staining method ;the apoptosis rate was examined by the flow cytometry ;the effects of PSAmRNA gene expression was detected by the FQ-RT-PCR .Results 5 ,10 ,15μmol/L cyclopamine groups had obvious inhibition effect on the LNCaP cell proliferation ,which had statistically significant difference compared with the control group (P<0 .01) .10 μmol/L group reached IC50 at 48 h ;the apoptosis rates at 24 ,48 ,72 h in the 10 ,15 μmol/L groups were 37 .21% , 57 .38% ,57 .98% and 21 .16% ,71 .31% ,72 .90% respectively ,the difference had statistically significant difference compared with the control group(P<0 .01) .The cellular apoptotic morphological changes were significantly enhanced with the increase of cyclo-pamine concentration and the extension of action time .The expression level PSAmRNA gene exhibited the obvious decreasing trend with the increase of cyclopamine concentration and was significantly decreased compared with the control group (P< 0 .01) .The PSAmRNA gene expression was extremely low in 10 ,15 μmol/L cyclopamine at different time period .Conclusion Cyclopamine significantly inhibits the proliferation of LNCaP cells ,induces apoptosis and obviously down-regulates the PSAmRNA gene expres-sion in LNCaP cells .Certain concentration of cyclopamine may be effective for treating advanced prostate cancer .

Objective To evaluate tension-free vaginal tape (TVT) procedure for women with ruinary incontience. Methods 37 patients with stress incontinence underwent the TVT procedure.An incision was made on the anterior vaginal wall and a curved trocar was introduced placing the TVT to the lower part of the middle urethra.An indevelling uretha catheter was with drained after 24 hours. Results The patients have been followed up for 3～16 months(mean follow-up of 13 months).All were cured by the TVT procedure.The main postoperative complication is retention (in 2 patients). Conclusions The results demonstrate that TVT procedure is safe and effective for the treatment of stress incontinence in female patients.

Objective To investigate the protective effect of Danhong Injection(DI) on rats ischemia-reperfusion(I/R) injury after kidney transplantation.Methods Ten normal SD rats were allocated into the sham-operation group,and they were only been anesthetized and exposed kidneys for 45 minutes.The allogeneic male SD rats model of kidney transplantation were established.The qualified rats were divided into 3 groups(10 in each group)at random:I/R model group,DI pretreatment group,and DI treatment group.B1ood urea nitrogen(Bun) and creatinine(Cr) levels in each group were determined at the 24th hour after operation.The expression of Bax and Bcl-2 in kidney were quantitatively detected by immunohistochemistry.Apoptotic index was measured by TUNEL method.Results Compared with the sham-operation group,the apoptosis rate(P

0.05.Only 1 of 28 (3.6%) showed positive survivin signal in the control group.The difference of the positive rate of survivin between groups of TCCB and control was significant ( P

OBJECTIVE:To observe the clinical efficacy of prostaglandin E1 used early in patients after renal transplantation. METHODS:93 renal transplant recipients (treatment group) were treated with PGE1(20 ?g?d-1) within 2 weeks after operation,and another 85 renal transplant recipients (control group) were not given PGE1 therapy in the same period. Urine volume,serum creatinine (Scr),blood flow resistance-indexes (RI) under Doppler color ultrasound,incidences of acute rejection (AR) and delayed graft function (DGF) and the 1 year patient/graft survival rates were compared between the two groups. RESULTS:The 24h urine volume in the treatment group on day 1 after operation was significantly higher than in the control group (9.40?1.9 L vs. 8.11?1.8L) (P