The role of neutrophil in the pathogenesis of many diseases attracts more attentions than before. Neutrophil elastase (NE), a powerful neutrol proteinase capable of causing major tissue destruction in many diseases, is mainly present in the azurophil granules of neutrophil. A solid-phase enzyme-linked immunosorbent-biotin assay for human NE has been developed. Only 20?l of plasma sample is needed. Non-smoking healthy adults have 33.7?6.7ng NE/ml plasma; patients with chronic obstructive pulmonary diseases, 48.0+ 10.9 ng NE/ml; patients with pyogenic dermatisis, 69.1?15.7 ng NE/ml; patients with systemic lupus erythematosus, 61.0?13.9 ng NE/ml; patients with aplastic anemia, only 12.3?5.3 ng NE/ml. All of these results have significant differences compared with the result of healthy adults. The results of assay showed that this quantitative assay with good specificity and accuracy may be a better criterion for investigating the effect of NE on pathogenesis of some diseases.

Objective To analyze the prescriptions containing Panlongqi Pieces for clinical application;To guide reasonable usage of medicine in clinic. Methods Adopted retrospective investigation method by selecting 1178 prescriptions of Chinese patent medicine containing Panlongqi Pieces from January to March in 2014. The unreasonable elements in these prescriptions were analyzed. Results Among the 1178 prescriptions, there were 585 unreasonable prescriptions, occupying 49.66%. Problems in the application of Panlongqi Pieces in clinic lie in differentiation of syndromes, combination of medication, incompatibility of medicine in prescription, and usage and dosage. Conclusion Doctors should attach importance to differentiation and reasonable usage in clinical application, which can achieve expected therapeutic effect.

Objective To analyze the usage of TCM decoction pieces in our hospital during 2011-2014;To provide references for clinical reasonable medicine using. Methods Statistic analysis was carried out from the aspects of annual amount of prescription, dosage, annual sales, and top 10 TCM decoction pieces among sales rank through management system of hospital pharmacy. Results Sales amount and dosage of TCM decoction pieces increased significantly. During the four years, six same TCM decoction pieces ranked among the dosage of top 10 TCM decoction pieces, namely Astragali Radix, Paeoniae Radix Alba, Angelicae Sinensis Radix, Salviae Miltiorrhizae Radix et Rhizoma, fried Atractylodis Macrocephalae Rhizoma, and Chuanxiong Rhizoma. The amount of prescription increased obviously. Conclusion TCM decoction pieces are commonly used in our hospital;dosage increased continuously;usage is generally reasonable.

Objective: To explore the effect of sequential external counterpulsation on sudden deafness.Methods: All the patients were treated with sequential external counterpulsation instrument produced by Guangzhou South-China Medical Instrument Company.Results: After treatment,the hearing threshold of 60 cases was all improved,with an increase from 5 to 53 db,and with an average threshold shift of 29?8.7db.86.7% of cases acquired satisfied therapeutic effects except 8 cases whose hearing threshold increased by less than 15db.Conclusion: the results showed that,the treatment of sudden deafness with Sequential External Counterpulsation has good therapeutic effects.Earlier and longer treatment means better effect

Objective To establish relevant management measures and guarantee the safety of clinical medication by analyzing the quality of warehouse entry inspection for Chinese decoction pieces.Methods According to the records of warehouse entry inspection for Chinese decoction pieces during January 2014 to December 2014, reasons for unqualified Chinese decoction pieces were collected and analyzed.Results Our hospital checked and accepted 2376 batches of Chinese decoction pieces, among which 98 batched were unqualified (4.12%); the reasons for unqualified Chinese decoction pieces mainly included improper storage, adulteration, and nonstandard preparation, which accounted for 48.97%, 19.39% and 18.37% respectively.Conclusion There are some problems in the Chinese decoction pieces in our hospital. Dispensary of TCM should enhance the management of check and acceptance of Chinese decoction pieces, and improve quality for check and acceptance and quality of Chinese decoction pieces.

Objective To survey the unconventional blood sugar level induced by quinolones and promote the rational drug use in clinical. Methods Collecting and analysis the unconventional blood sugar level induced by quinolones reported during the period of 1995 ～ 2008 using CHKD net. Results 107 cases of unconventional blood sugar level were reported by using 9 kinds of quinolones and among them 70 cases with hypoglycemia and 3 cases died of hypoglycemic coma. Another 37 cases were found with hyperglycemia. Conclusion Should reinforce the supervi-sion of rational use of quinolones and monitor the blood sugar level when the elderly and the diabetic of renal insuffi-ciency in clinical use.

AIM: To study the efficacy and adverse reactions of venlafaxine in the treatment of generalized anxiety disorder.　METHODS: Twenty-one patients (M 13, F 8; age 33 a± s 16 a) with diagnosis of generalized anxiety disorder according to CCMD-Ⅱ-R were treated with venlafaxine 50 mg*d-1 for 4 wk. Patients were assessed with the HAMA and TESS and routine experimental examination before and after treatment.　RESULTS: Venlafaxine was obvious effective to generalized anxiety disorder. The effectiveness appeared after 1 wk. The remarkable effective rate was 76% at 4 wk. The main adverse reactions of venlafaxine were thirst, sweating nausea and headache etc. 　CONCLUSION: Venlafaxine is an effective and safe drug in the treatment of generalized anxiety disorder.

BACKGROUND: Several studies have demonstrated that degradable polycaprolactone film has no apparent influences on cellular immune function of rat critical organs including heart, liver, and kidney, and possesses the prerequisite of anti-adhesion formation.OBJECTIVE: To investigate the role of degradable polycaprolactone film in preventing rat peritoneal adhesion following surgery.DESIGN, TIME AND SETTING: The present randomized, controlled animal experiment was performed at the Department of Physiology, Jilin University.MATERIALS: A total of 120 male Wistar rats, weighing 180-220 g, were included in this study. Peritoneal adhesion was induced in all rats. Non-bio-degradable film was sourced from Wuhan Boster Company, China. Polycaprolactone film was purchased from U.C.C Company. England.METHODS: All 120 rats were randomly divided into 3 groups, with 40 rats per group: control, non-bio-degradable film, and polycaprolactone film. Peritoneal adhesion was induced in all rats. The non-bio-degradahle film and polycaprolactone film groups were subjected to peritoneal wound cover with non-bio-degradable film and polycaprolactone film, respectively. From day 1 following surgery, each rat daily received an intraperitoneal injection of 4 mL 2 g/L pseudomonas enzyme, for a total of 3 successive days.MAIN OUTCOME MEASURES: At 1, 3, 7, and 30 days following surgery, 10 rats were selected from each group for observation of peritoneal adhesion.RESULTS: Totally 120 rats were included in the result analysis. Peritoneal adhesion was successfully induced in rats from the control group. From day 7 following surgery, the peritoneal adhesion rate was significantly lower in the polycaprolactone film group than in the control group (P<0.01). In the non-bio-degradable film group, the peritoneal adhesion rate exhibited decreasing followed by increasing tendency; by day 30, the adhesion rate was significantly higher compared to the polycaprolactone film group (P<0.01).CONCLUSION: Polycaprolactone film is degraded by pseudomonas enzyme to avoid the new adhesion caused by foreign stimulation.

OBJECTIVE:To study the cooperative effect of arsenic trioxide(As 2 O 3 )and octreotide on the proliferation of human hepatocellular carcinoma(HCC)SMMC-7721and to observe the combining effect of the two drugs.METHODS:The killing effect of drugs was determined by MTT;the cell cycle was determined by flow cytometry(FCM);the drug combination analysis was conducted by the moderate efficiency principle.RESULTS:The combination of arsenic trioxide and octreotide significantly inhibited cell proliferation of SMMC-7721in vitro;and the moderate efficiency concentration of As 2 O 3 and oc-treotide in combination was2.022?mol/L and8.088?mol/L respectively,which were lower than those when used singly.The combination of two drugs can inhibit HCC at the S-phase.The combination index was less than1if the combining domino offect of As 2 O 3 and octreotide was more than0.3.CONCLUSION:Combination of As 2 O 3 and octreotide in vitro can signifi-cantly increase the inhibition effect of each single one on the proliferation of SMMC-7721and the combination of two has a better anticarcinogenic effect when the domino offect of the combination is above0.3.

Objective To evaluate the effect of “T”incision on distant stereovision of cataract patients with high cornea astigma-tism .Methods 40 age-related cataract patients (40 eyes) with high cornea astigmatism for surgery in our hospital were divided into two groups :A(20 eyes) an B(20 eyes) .The A groups received superior(11 :00) clear corneal incision(CCI) .The B groups besides the superior(11:00) CCI ,for surgery on the steepest meridian ,an additional couple of “T”incisions were made to release the preex-isting corneal astigmatism .The operation eyes were all implanted Rayner foldable posterior chamber intraocular lens .The UCVA , corneal astigmatism ,distant stereovision were observed at 3 months after operation .Results The UCVA in each group after sur-gery was all better than that of preoperation ,difference being statistically significant (P<0 .01) .the difference between Group A and Group B was significant(P<0 .05);In Group A ,there were no significant difference on the corneal J0 and P before and after op-eration(P>0 .05) ,The corneal J0 and P in group B after surgery were lower than that of preoperation .difference being statistically significant(P<0 .01) .difference between Group A and Group B(P<0 .01) at 3 months postoperation was statistically significant (P<0 .01);The stereopsis in each group after surgery was all better than that of preoperation ,difference being statistically significant (P<0 .01) .the difference between Group A and Group B postoperation was significant (P<0 .05) .Conclusion the“T” incisions on the steepest corneal meridian can improve the patient′s visual function and distant stereovision .