Objective To analyze the usage of TCM decoction pieces in our hospital during 2011-2014;To provide references for clinical reasonable medicine using. Methods Statistic analysis was carried out from the aspects of annual amount of prescription, dosage, annual sales, and top 10 TCM decoction pieces among sales rank through management system of hospital pharmacy. Results Sales amount and dosage of TCM decoction pieces increased significantly. During the four years, six same TCM decoction pieces ranked among the dosage of top 10 TCM decoction pieces, namely Astragali Radix, Paeoniae Radix Alba, Angelicae Sinensis Radix, Salviae Miltiorrhizae Radix et Rhizoma, fried Atractylodis Macrocephalae Rhizoma, and Chuanxiong Rhizoma. The amount of prescription increased obviously. Conclusion TCM decoction pieces are commonly used in our hospital;dosage increased continuously;usage is generally reasonable.

Objective To analyze the prescriptions containing Panlongqi Pieces for clinical application;To guide reasonable usage of medicine in clinic. Methods Adopted retrospective investigation method by selecting 1178 prescriptions of Chinese patent medicine containing Panlongqi Pieces from January to March in 2014. The unreasonable elements in these prescriptions were analyzed. Results Among the 1178 prescriptions, there were 585 unreasonable prescriptions, occupying 49.66%. Problems in the application of Panlongqi Pieces in clinic lie in differentiation of syndromes, combination of medication, incompatibility of medicine in prescription, and usage and dosage. Conclusion Doctors should attach importance to differentiation and reasonable usage in clinical application, which can achieve expected therapeutic effect.

The role of neutrophil in the pathogenesis of many diseases attracts more attentions than before. Neutrophil elastase (NE), a powerful neutrol proteinase capable of causing major tissue destruction in many diseases, is mainly present in the azurophil granules of neutrophil. A solid-phase enzyme-linked immunosorbent-biotin assay for human NE has been developed. Only 20?l of plasma sample is needed. Non-smoking healthy adults have 33.7?6.7ng NE/ml plasma; patients with chronic obstructive pulmonary diseases, 48.0+ 10.9 ng NE/ml; patients with pyogenic dermatisis, 69.1?15.7 ng NE/ml; patients with systemic lupus erythematosus, 61.0?13.9 ng NE/ml; patients with aplastic anemia, only 12.3?5.3 ng NE/ml. All of these results have significant differences compared with the result of healthy adults. The results of assay showed that this quantitative assay with good specificity and accuracy may be a better criterion for investigating the effect of NE on pathogenesis of some diseases.

Objective To establish relevant management measures and guarantee the safety of clinical medication by analyzing the quality of warehouse entry inspection for Chinese decoction pieces.Methods According to the records of warehouse entry inspection for Chinese decoction pieces during January 2014 to December 2014, reasons for unqualified Chinese decoction pieces were collected and analyzed.Results Our hospital checked and accepted 2376 batches of Chinese decoction pieces, among which 98 batched were unqualified (4.12%); the reasons for unqualified Chinese decoction pieces mainly included improper storage, adulteration, and nonstandard preparation, which accounted for 48.97%, 19.39% and 18.37% respectively.Conclusion There are some problems in the Chinese decoction pieces in our hospital. Dispensary of TCM should enhance the management of check and acceptance of Chinese decoction pieces, and improve quality for check and acceptance and quality of Chinese decoction pieces.

Objective: To explore the effect of sequential external counterpulsation on sudden deafness.Methods: All the patients were treated with sequential external counterpulsation instrument produced by Guangzhou South-China Medical Instrument Company.Results: After treatment,the hearing threshold of 60 cases was all improved,with an increase from 5 to 53 db,and with an average threshold shift of 29?8.7db.86.7% of cases acquired satisfied therapeutic effects except 8 cases whose hearing threshold increased by less than 15db.Conclusion: the results showed that,the treatment of sudden deafness with Sequential External Counterpulsation has good therapeutic effects.Earlier and longer treatment means better effect

A 69-year-old male patient presented with a gradually enlarging mass in the left inner upper thigh for more than 2 months,and pigmented patches in the left medial leg for more than 20 years.Physical examination revealed a painless mass measuring 3 cm × 2 cm × 2 cm in size in the left inner upper thigh.Several pigmented patches were observed in the left medial leg,and the largest pigmented patch measured 2 cm× 2 cm in size with an irregular border and uneven pigmentation.The mass in the left inner upper thigh was resected and subjected to histopathological examination,which showed proliferative epithelioid neoplastic cells with mucous matrix,round and spindle cells of varying sizes separated by mucous matrix.The immunohistochemical study of tumor cells showed positive staining for vimentin,S100 and Melan-A,but negative staining for actin,desmin,CD56,epithelial membrane antigen,cytokeratin,leukocyte common antigen,CD99,chromogranin A and synaptophysin.Hematoxylin-eosin staining of pigmented patches on the left medial leg revealed squamous epithelium covering the surface of lesions with no superficial ulceration or atypia in epithelial cells,unevenly distributed melanophages,fibroplasia accompanied by collagen formation,obviously decreased skin appendages,infiltration of a few inflammatory cells in the dermis.AB-PAS staining was negative.The immunohistochemical study of pigmented patches showed positive staining for vimentin and Melan-A.The patient was pathologically diagnosed with metastatic myxoid melanoma with partial regression of the primary lesion.

Objective To describe the clinicopathologic features of two cases of mucoepidermoid carcinoma of the skin.Methods Two cases of mucoepidermoid carcinoma of the skin were analyzed histopathologically using hematoxylin and eosin (HE) staining,alcian blue-periodic acid Schiff (AB-PAS) staining and immunohistochemical staining.Relavant literature was reviewed.Results Histopathological examination showed that the tumor was subcutaneously located in both cases,with epidermoid cells and intermediate cells arranged in sheets or nests,as well as different sizes of glandular structures lined by mucinous columnar epithelium in some areas.Both tumors had a relatively clear boundary with peripheral invasive growth and no obvious capsules.Immunohistochemical examination showed positive staining for carcinoembryonic antigen (CEA),high and low molecular weight cytokeratin (CK(H) and CK(L)).The cytoplasm of mucous cells was stained blue with,and mucus was visualized after,AB-PAS staining.Conclusions Primary mucoepidermoid carcinoma of the skin is a kind of malignant tumor arising from skin appendages,whose diagnosis depends on histological and immunohistochemical examination.

Objective To develop an immunohistochemical assay for the diagnosis of cutaneous malignant melanoma (CMM) micrometastasis via blood and lymphatic vessels,and to evaluate its clinical significance.Methods Fifty-three patients (32 males and 21 females) histopathologically diagnosed as CMM were enrolled in this study.The patients were aged (61.2 ± 8.4) years (range,52-72 years).Tissue specimens were obtained from the central area of tumor in each case,and also from removed lymph nodes in some cases.The average duration of follow-up was (65.00 ± 5.68) months.During the follow-up,17 patients died of the recurrence or metastasis of CMM,and 6 patients were lost to follow-up.The expressions of D2-40,S100 and CD34 antigens in 53 tissue specimens were examined by immunohistochemical staining with three individual monoclonal antibodies,or by an immunohistochemical method using 2 two-antibody cocktails (D2-40/S 100 and CD34/S100) and double-color chromogens in single tissue sections.Results Of the 53 patients,30.19％ (16/53) were positive for hematoxylin-eosin (HE) staining combined with immunohistochemical staining with individual monoclonal antibodies,and 49.06％ (26/53) for the immunohistochemical method using two-antibody cocktails and double-color chromogens.Statistical differences were found in the positive rate between the two methods (x2 =3.94,P＜ 0.05).Compared with patients without blood/lymph vessel tumor emboli,those with blood/lymph vessel tumor emboli showed higher lymph node metastasis rate (80.77％ (21/26) vs.37.04％ (10/27),x2 =10.43,P ＜ 0.001),but lower five-year survival rate (42.31％ (11/26) vs.70.37％ (19/27),x2 =4.25,P ＜ 0.05).Conclusions The immunohistochemical method with two-antibody cocktails is superior to HE staining combined with immunohistochemical staining with individual monoclonal antibodies in the detection of blood/lymph vessel tumor emboli.And blood/lymph vessel tumor emboli may be an important prognostic factor in patients with CMM.

Objective To evaluate the effect of “T”incision on distant stereovision of cataract patients with high cornea astigma-tism .Methods 40 age-related cataract patients (40 eyes) with high cornea astigmatism for surgery in our hospital were divided into two groups :A(20 eyes) an B(20 eyes) .The A groups received superior(11 :00) clear corneal incision(CCI) .The B groups besides the superior(11:00) CCI ,for surgery on the steepest meridian ,an additional couple of “T”incisions were made to release the preex-isting corneal astigmatism .The operation eyes were all implanted Rayner foldable posterior chamber intraocular lens .The UCVA , corneal astigmatism ,distant stereovision were observed at 3 months after operation .Results The UCVA in each group after sur-gery was all better than that of preoperation ,difference being statistically significant (P<0 .01) .the difference between Group A and Group B was significant(P<0 .05);In Group A ,there were no significant difference on the corneal J0 and P before and after op-eration(P>0 .05) ,The corneal J0 and P in group B after surgery were lower than that of preoperation .difference being statistically significant(P<0 .01) .difference between Group A and Group B(P<0 .01) at 3 months postoperation was statistically significant (P<0 .01);The stereopsis in each group after surgery was all better than that of preoperation ,difference being statistically significant (P<0 .01) .the difference between Group A and Group B postoperation was significant (P<0 .05) .Conclusion the“T” incisions on the steepest corneal meridian can improve the patient′s visual function and distant stereovision .

OBJECTIVE:To study the cooperative effect of arsenic trioxide(As 2 O 3 )and octreotide on the proliferation of human hepatocellular carcinoma(HCC)SMMC-7721and to observe the combining effect of the two drugs.METHODS:The killing effect of drugs was determined by MTT;the cell cycle was determined by flow cytometry(FCM);the drug combination analysis was conducted by the moderate efficiency principle.RESULTS:The combination of arsenic trioxide and octreotide significantly inhibited cell proliferation of SMMC-7721in vitro;and the moderate efficiency concentration of As 2 O 3 and oc-treotide in combination was2.022?mol/L and8.088?mol/L respectively,which were lower than those when used singly.The combination of two drugs can inhibit HCC at the S-phase.The combination index was less than1if the combining domino offect of As 2 O 3 and octreotide was more than0.3.CONCLUSION:Combination of As 2 O 3 and octreotide in vitro can signifi-cantly increase the inhibition effect of each single one on the proliferation of SMMC-7721and the combination of two has a better anticarcinogenic effect when the domino offect of the combination is above0.3.