To investigate the mechanism of Xiefei Dingchuan injection (XFDCI,泻肺定喘注射液) in treating pulmonary hypertension by means of measuring the activity of Ca2＋Mg2＋ ATPase and Na＋K＋ATPase of red blood cell(RBC) membrane.Methods:The pulmonary hypertension models induced by monocrotaline in 18 rats were established then treated with XFDCI.Using cardiac catheter and chromatometry respectively the pulmonary artery pressure (PAP) and the activities of Ca2＋Mg2＋ATPase and Na＋K＋ATPase of RBC membrane were measured.Results:The activities of Ca2＋Mg2＋ATPase and Na＋K＋ATPase of RBC membrane in XFDCI group were obviously higher than those in pulmonary hypertension group.Conclusions:XFDCI is able to reduce the pulmonary hypertension and its mechanisms may be related to increasing the activities of Ca2＋Mg2＋ATPase and Na＋K＋ATPase of RBC membrane.

Some hormones and glucocorticoid in chronic strss feedback are demonstrated to have significant effects on cancer progression.Recently,as a typical neuropeptide,gastrin releasing peptide (GRP) could mediate chronic stress to promote carcinogenesis through four conceivable mechanisms,such as regulating circadian rhythms,suppressing immunity system,acting as a mitogenic effect and mediating cancer-related psychiatric disorders,et al.

The paper discusses the difference in postgraduate training between China and the United States,intending to supply some advanced experiences for Chinese postgraduate education reform.

Objective:To develop a HPLC method for determination of Podophyllotoxin in two preparations.Methods:HPLC was carried out,using a Dikma diamonsil C18 column(4.6mm?150mm,5?m)with a column temperature at 25℃,the methanol-water as mobile phase(using gradient)with flowing rate 1.0 mL/min.The detection wavelength was at 292 nm.Results:The method had good linear relationship within the range of 11.8-590?g/mL for Podophyllotoxin,with the correlation coefficients 0.9999.The results of the average recovery of Youdi liniment and Podophyllotoxin tincture were 99.38%,99.62%.Conclusion:The rapid,accurate and reproducible RP-HPLC method was useful for quantification of Podophyllotoxin in Youdi Liniment and Podophyllotoxin Tincture.

Objective To discuss the clinical manifestations and imaging diagnostic value of tuberous sclerosis accompanied by multi-organ injuries.Methods 2 cases in our hospital and 27 cases of tuberous sclerosis from the published reports were collected.The clinical manifestations and imaging features as well as the extent and type of multi-organ lesion were analyzed.Results Among the 29 patients,17 were male and 12 were female.The averaged age was 16.8.The clinical manifestations included:with family history in 6 , epilepsies in 25 (15 with mental retardation),symmetric angiofibroma in the face in 22 (3 with similar changes in the else body surface),fibromas beneath toenail or fingernail in 4, hyperdactylia of thumb in 2 and abdominal masses in 7 (4 with hematuria), tuberous scleroses beneath ependymal layer in 27,17 patients with kidney diseases,9 involved liver,5 involved spleen.Conclusion Multiple organs can be involved by tuberous sclerosis.The calcification of ependymal lyer and harmatoma of kidney are the most valuable in imaging diagnosis of the disease.

Objective To explore the clinical value of interferon gamma release assays in early childhood diagnosis of latent tuberculosis infection.Methods 33 patients with suspected latent tuberculosis infection and 35 patients with active tuberculosis infection in our hospital from January 2012 to March 2013 were selected.During the same period selected 30 healthy children as a control group.Respectively were given sputum smear analysis,tuberculin skin test (TST)and vinterferon release assay (IGRA).Compare the positive rate of different methods. Results In suspected latent tuberculosis group,IGRA test results were significantly higher than TST and sputum smear method(P<0.05);in active tuberculosis infection group, IGRA test results were significantly higher than TST and sputum smear(P<0.05 );in healthy control group,TST test results were higher than the IGRA,it is because of patients had been vaccinated with BCG.Conclusion IGRA can be used as one way to diagnosis the latent tuberculosis infection.

Objective To investigate epidemic status and explore the influential factors of pulmonary tuberculosis (PTB) in the servicemen of armed forces and to provide reference basis for control and prevention. Methods According to the questionnaire, the test of PPD (purified protein derivative), X-ray checking and sputum bacteria culture method had been used. A 1∶1 matched case-control study was carried out in the servicemen with 162 cases and 162 controls. Odds ratios (ORs) were calculated for the relevant factors. Results The prevalence rate of activated tuberculosis, the bacteriological positive prevalence rate, the smear positive prevalence rate and PPD positive reaction rate respectively were 176.66/105, 33.12/105, 22.08/105 and 34.31%. The prevalence rate of officers was obviously higher than that of soldiers, and the army resident in the countryside was higher than that in the city. The prevalence rate of high training intensity was higher than that of low training intensity. With the increasing of the age and time duration in army, the prevalence rate of pulmonary tuberculosis and the positive rate of PPD reaction presented a ascending tendency. Single factor conditional logistic regression analysis showed that the occurrence of PTB was closely associated with training intensity, body mass index, the number of scar, housing condition, the contact history of infection source, area of enrollment and character, their ORs value were 5.12, 0.37, 0.42, 0.51, 1.82, 0.57 and 1.63 respectively (P

Objective To study basic thyroid stimulating hormone (bTSH) levels impact on outcomes of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) in Qinghai.Methods Totally 282 cases with IVF cycles and 93 cases with ICSI cycles were studied prospectively,according to bTSH level,patients were divided into four groups.Reproduction rate,clinical pregnancy rate,miscarriage rate and live birth rate were studied among four groups.Results (1) In 375 cases with IVF/ICSI cycles,bTSH was positively correlated with abortion rate (r=0.42,P=0.04),but live birth rate and growing rate showed negative correlations with bTSH (r=-0.42,-0.28; P=0.04,0.03).bTSH and the number of eggs,the number of fertilized eggs,the number of embryos,biochemical pregnancy rate,and clinical pregnancy rate were no significant correlation (all P＞0.05).(2) Among women at group of ≤1.7,＞1.7 and ≤2.5,＞2.5 and ≤3.5,＞3.5 mU/L,the implantation rates were 28.7％,27.3％,37.7％ and 19.2％,live birth rates were 80.9％,75.0％,82.7％,and 59.8％,abortion rates were 19.0％,15.0％,16.7％,40.1％; they all showed significant difference (all P＜0.05).Abortion rate in women with high bTSH level was higher than that of women with lower bTSH level,however implantation rate,live birth rate in women with high bTSH level were lower.Conclusion When bTSH level is ＞3.5 mU/L,the abortion rate were increased,but live birth rate,rate of implantation were decreased.

Objective To analyze the epidemiological characteristics for new detecting cases of leprosy in 2012, in Yunnan Province, and provide clue and foundation for prevention and treatment in leprosy control. Methods The date of diagnosed leprosy patients in 2012 in Yunnan Province were collected and analyzed by disease reporting information system. Results 230 new cases were founded in 2012, and the discovery rate was 0.50/100 000. 4.35% of new cases were children, 64.35% of new cases were MB and 19.57% had grade 2 disability. 17 recurrent cases were founded in 2012,and 6 of them had received MDT. By the end of 2012, there were still 1084 present case in Yunnan Province,the prevalence rate was 0.24/10 000,and 485 of them need MDT. Conclusion The prevalence of leprosy was decreased,and the prevalence varies a lot in different regions. Honghe and Wenshan are still the focus regions. Leprosy is still a serious public health and social problem in Yunnan Province. In order to reduce the burden of leprosy and eliminate the leprosy danger, long-term financing investment and prevention are still needed.

Objective To study the effects of using the dropping retention-enema in the clinical practices.Methods Divided 60 cases who need retention-enema into the experimental group and the control group,there were 30 cases in the each group.The traditional retention-enema method was used in the control group,while the dropping retention-enema method was used in the experimental group.Compared the related factors between the two groups.Results All the factors which can indicated the clinical effects in the experimental group were better than those of in the control group,P