1.Experimental Study on the Cooperative Anticarcinogenic Effect of Arsenic Trioxide and Octreotide on Human Hepatocellular Carcinoma
China Pharmacy 2001;0(07):-
OBJECTIVE:To study the cooperative effect of arsenic trioxide(As 2 O 3 )and octreotide on the proliferation of human hepatocellular carcinoma(HCC)SMMC-7721and to observe the combining effect of the two drugs.METHODS:The killing effect of drugs was determined by MTT;the cell cycle was determined by flow cytometry(FCM);the drug combination analysis was conducted by the moderate efficiency principle.RESULTS:The combination of arsenic trioxide and octreotide significantly inhibited cell proliferation of SMMC-7721in vitro;and the moderate efficiency concentration of As 2 O 3 and oc-treotide in combination was2.022?mol/L and8.088?mol/L respectively,which were lower than those when used singly.The combination of two drugs can inhibit HCC at the S-phase.The combination index was less than1if the combining domino offect of As 2 O 3 and octreotide was more than0.3.CONCLUSION:Combination of As 2 O 3 and octreotide in vitro can signifi-cantly increase the inhibition effect of each single one on the proliferation of SMMC-7721and the combination of two has a better anticarcinogenic effect when the domino offect of the combination is above0.3.
2.Clinical Values of Blood Serum Concentration of MHD in Children with Epilepsy of Different Ages
Xia LI ; Xiaohong LIU ; Le YANG
Journal of Modern Laboratory Medicine 2015;(1):143-144,148
Objective To investigate effects of blood serum concentration of MHD in children with epilepsy of different ages. Methods Two hundred children with epile psy were divided into four groups according to age,group A:<3 years old in-fants;group B:3~6 years old preschoolers;group C:7~13 years old school-age chidren.Group D:14~18 years old adoles-cents,each group had 50 chidren.The serum concentration of active metabolite was detected by high-perform ance liquid chromatography (HPLC)method.Results The serum concentration of MHD increase with age,but there were no statistical significance (P>0.05).Conclusion The indiv idual variation in blood serum concentrations of MHD in children with epi-lepsy adminisered with oxcarbazepine is high,therefore monitoring the blood serum concentration is helpful to adj ust the dose of medication and to promote the implementation of the individualized dosing regimens.
3.Thoughts on university libraries'providing digital information resources services for college students
Xiaohong XIA ; Lei JIN ; Meng HUANG
Chinese Journal of Medical Education Research 2005;0(06):-
In most universities the usage of digital information resources are not ideal.One reason is that primary audience groups of libraries——college students rarely use these resources.So we propose some measures and proposals for university libraries to provide the service of digital information resources for university students.
4.Combination of ultrasonography and magnetic resonance image in diagnosing monochorionic multifetal malformations-report of 14 cases
Xia ZHU ; Xinlin CHEN ; Xiaohong YANG ; Ziyan SUN ; Mei XIAO ; Liming XIA ; Xiaohong ZHU
Chinese Journal of Perinatal Medicine 2010;13(3):196-200
Objective To demonstrate the value of eombined application of prenatal ultrasonography with fetal magnetic resonance imaging(MRI) in the diagnosis of monochorionic muhifetal realformations. Methods Fourteen cases of muhifetal malformations,detected by prenatal ultrasonography,received MRI within 48 h afterwards.All diagnosis were confirmed after delivery or mid-term termination.All imaging results of the 14 cases were retrospectively reviewed. Results Among the 14 cases,there were 7 acardias,5 Conjoined twins and 2 demise of multifetuses.Comparing ultrasound with MRI,we found that:(1)In cases with acardia and demise of multifetusea,ultrasound could diagnose correctly and be an important tool for follow-up,while MRI could demonstrate organs and structures of the acardiac recipient more clearly and detect the secondary changes of brain in the donor and survived fetus.(2)In Conjoined twins,ultrasound was superior to MRI in demonstrating the structure and function of cardiovascular system : and equivalent to MRI in identifying stomach,kidney,bladder and limbs;but inferior to MRI in identifying esophagus,lung,liver and intestinal,especially in the brain. And MRI could demonstrate two fetuses and the relationship between them in COnjoined twins simultaneously. Conclusions Prenatal ultrasonography and MRI have their own advantages and disadvantages in diagnosing monochorionic multifetal malformations.But the combination of prenatal ultrasonography and fetal MRI may be more valuable.
5.Protective effect of diphenyleneiodonium, a NADPH oxidase inhibitor, on hyperpermeability of endothelial cells exposed to AOPP-HSA in vitro
Ying ZHANG ; Xueying XIA ; Chunxiao WANG ; Xiaohong WANG ; Hequn ZOU
Chinese Journal of Pathophysiology 2015;(7):1172-1177
[ ABSTRACT ] AIM: To investigate the effect of advanced oxidation protein product-human serum albumin ( AOPP-HSA) at different concentrations on the permeability of human umbilical vein endothelial cell ( HUVEC) monolayer and the protective effect of NADPH oxidase inhibitor diphenyleneiodonium ( DPI ) against AOPP-HSA exposure. METHODS: Cultured HUVECs were exposed to 200 mg/L HSA (control) or AOPP-HSA (50, 100 and 200 mg/L).The permeability of the endothelial monolayer was assessed by measuring CMFDA-labeled THP-1 cells across the endothelial cells.The cultured HUVECs were treated with HSA (200 mg/L), AOPP-HSA (200 mg/L), or AOPP-HSA (200 mg/L)+DPI (100 μmol/L), and the activation of NADPH oxidase, endothelial monolayer permeability and cytoskeleton rear-rangement were evaluated.RESULTS: AOPP-HSA increased the permeability of the endothelial cell monolayer, and AOPP-HSA at 200 mg/L significantly increased the phosphorylation level of NADPH oxidase in the cells.Treatment with 100 μmol/L DPI obviously attenuated AOPP-HSA-induced NADPH oxidase activation, the increase in the permeability of the cell monolayer and the cytoskeleton rearrangement.CONCLUSION: AOPP-HSA increases the hyperpermeability of HUVEC monolayer via the phosphorylation of NADPH oxidase, and the NADPH oxidase inhibitor DPI reverses such effects.
6.Combined measurement of serum CYFRA21-1,NSE,CEA,CA19-9,CA125 and SCC:diagnosis value to lung cancer
Yimin ZHANG ; Wenjin XIA ; Caiping MAO ; Xiaohong XU
China Oncology 1998;0(04):-
Background and purpose:Measurement of tumor markers in serum is widely used for the diagnosis of lung cancer, but the positive rates of single marker for diagnosis of lung cancer were not high. This study was to investigate the clinical value of combined measurement of serum CYFRA21-1,NSE,CEA,CA19-9,CA125 for the diagnosis of lung cancer. Methods:The levels of CYFRA21-1,NSE,CEA,CA19-9,CA125,SCC were assayed and compared between the lung cancer group(135 cases) and cancer-free control group(20 case) by electrochemiluminesence immunoassay.Results:The levels of CYFRA21-1,NSE,CEA,CA19-9,CA125 and SCC in the lung cancer group were (7?8) ng/ml,(30?29) ng/ml,(65?293) ng/ml,(110?379) U/ml,(122?412) U/ml and (2?7) ng/ml, respectively, which were higher than those in the control group. The levels of the six tumor markers were associated with pathology of lung cancer, the levels of CEA were (11?25)?10 ng/ml,(2?4) ?10 ng/ml,(2?3)?10 ng/ml in the adenocarcinoma , squamous cell carcinoma and small cell carcinoma cases respectively; the levels of CA125 were (21?48)?10 U/ml,(48?57) ?10 U/ml,(5?4)?10 U/ml in the adenocarcinoma ,squamous cell carcinoma and small cell carcinoma patients, respectively; the levels of CA199 were (17?44) ?10 U/ml,(5?12)?10 U/ml,(4?4) ?10 U/ml in the adenocarcinoma, squamous cell carcinoma and small cell carcinoma patients, respectively. The levels of CEA,CA125,CA19-9 in the adenocarcinoma were higher than those in the squamous cell carcinoma and small cell carcinoma, but there was no significance different(P
7.Influencing Factors of Working Enthusiasm among Public Hospital Medical Staff in Underdeveloped Area
Xiaohong XIA ; Xiong KE ; Huiping ZHANG ; Yanchun WU
Chinese Medical Ethics 2017;30(3):372-375
Objective:To explore the influencing factors of working enthusiasm among public hospital medical staff in underdeveloped areas.Methods:With the method of stratified sampling,a questionnaire survey was conducted among 310 public hospital medical staff.The influencing factors of working enthusiasm were analyzed using the method of factor analysis and Ordinal Logistic model.Results:From the 14 incentives,we extracted three common factors including relationship development,living,and leisure.After the analysis of Ordinal Logistic model,it showed that the main influencing factors of the enthusiasm were the relationship development,leisure and hospital grade.Conclusion:It should put medical staff member leave system into effect to create a good atmosphere for relationships,and provide a reasonable space for promotion to enhance the enthusiasm of medical staff.
8.Effects of NO and NO synthetase inhibitor on endogenous endothelin secretion in acute renal failure in rats
Xiaohong XIA ; Yuxue SHEN ; Jixin SUN ; Jicai SHI
Chinese Journal of Pathophysiology 1986;0(04):-
The effects of L-arginine (L-Arg), the physiological NO precureor and NO synthetase inhibitor NG-nitro-L-arginine (L-NNA) on endogenous endothelin (ET) secretion and renal function were observed in a model of glycerol-induced acute renal failure (ARF) in rat. It was found that endogenous ET secretion was significantly increased, while serum NO was markedly decreased in ARF rats. The administration of L-NNA to the ARF rats induced significant increases in plasma ET and positive immunoreactive particles of ET in renal tubular epithelial cells(EP cells), and the impairment of renal function was exaggerated. L-Arg might effectively decrease the level of plasma ET and the positive immunoreactive particles of ET in renal tubular EP cells, suggesting the improvement of the renal function. It is suggested that the ability of NO in improving renal function may relatedto the inhibition of endogenous ET secretion in glycerol-induced ARF rats.
9.Influence of open access journals of biomedicine in China on academic exchanges
Chunguang WENG ; Cheng HUANG ; Tingchao XIAO ; Xiaohong XIA ; Zhun LI
Chinese Journal of Medical Education Research 2012;11(7):758-762
Objective To compare the influence of open access journals ( OAJ ) of biomedicine on academic exchanges by empirically analyzing the journals enrolled in the Chinese science citation database-the core (CSCD-C).Methods Multivariate statistical analysis was performed on OAJ and non OAJ in CSCD by using bibliometric and statistical methods,SPSS software and rank-sum test.Results The OAJ were accounted for only 31.63% of the total enrolled journals,the average ratio of funded papers in OAJ was 72.29%.Statistical journals had high influence in the discipline.The selfcitation rates of clinical medicine OAJ and special medical OAJ were high.The average impact factor and h index in biological OAJ were high.The average impact factor of preventive medicine OAJ was high.The average h index of comprehensive non OAJ was high.Conclusions The distribution of OAJ is uneven among different disciplines.The ratio of funded papers is higher in OAJ than in non OAJ with insignificant differences in discipline influence.
10.Evaluation on web impact of foreign medical open access journal websites based on link analysis method
Cheng HUANG ; Chunguang WENG ; Xiaohong XIA ; Tingchao XIAO
Chinese Journal of Medical Education Research 2012;(11):1192-1196
Web impact of 30 foreign biomedical OA journal websites was evaluated using link analysis method.The current situation and existing problems of foreign medical OA Journal websites were discussed combined with evaluation results in order to provide references for the construction of medical OA journal websites in China.