Ambulatorybloodpressuremonitoring(ABPM)providesmoreextensiveclinical information than clinical casual blood pressure. It can effectively identify w hite coat hypertension and masked hypertension and is superior to clinical casual blood pressure in predicting stroke risk. Both daytime and nighttime systolic blood pressures are the independent predictors of stroke, and the predictive value of nighttime systolic blood pressure is higher. In addition, ABPM can also be used to evaluate the blood pressure response and control during the primary and secondary stroke prevention. This article review s the application of ABPM in stroke prevention.

Objective:To observe the effects of gallnut water extract on the vitality of plaque biofilms.Methods:The oral plaque biofilms were obtained through bonding the enamel fragments to the bucca of the mindibular first molar for 24 h.The two groups of the biofilms were treated by gallnut water extract at 6 mg/ml and saline(control) for three minutes respectively.The effect of gallnut water extract on the vitality of plaque biofilms was observed by Ethidium bromide/Fluorescein diacetate (EB/FDA) staining and confocal laser scan microscope(CLSM).Results:All specimens had early biofilm formed,The percent vitality of the plaque biofilms treated with gallnut water extract and the control was 37.10?9.63 and 60.78?7.60 respectively(P

Objective To study the heart rate variability(HRV) and blood pressure variability(BPV) in patients with different grades of hypertension.Methods 120 cases of patients with hypertension,according to 1999 WHO/ISH hypertension diagnostic criteria are:1 hypertension in 40 cases,2 of hypertension in 40 cases,3 of hypertension in 40 cases and 40 cases of normal control group were detected heart rate variability and blood pressure variability.Results HRV was significantly different between the two groups(P <0.05 or p <0.01).The blood pressure increased and HRV tended to decrease.The BPV was significantly different(P < 0.01) between the two groups;the blood pressure increased and the BPV tended to increase.Conclusion The grade of hypertension is related to HRV and BPV.

OBJECTIVE:To establish a method for the simultaneous determination of chlorogenic acid,paeoniflorin,ferulic ac-id,berberine hydrochloride and baicalin in Lianqiao baidu pill. METHODS:HPLC was performed on the column of Agilent Eclipse XDB-C18 with mobile phase of 0.5% phosphoric acid(triethylamine adjusted to pH 3.0)-methanol(gradient elution)at a flow rate of 1.0 ml/min,the column temperature was 30 ℃,the detection wavelength was 230 nm(for paeoniflorin and berberine hydrochlo-ride),324 nm(for chlorogenic acid and ferulic acid)and 278 nm(for baicalin),and the injection volume was 5 μl. RESULTS:The linear range was 1.884-30.144 μg/ml for chlorogenic acid(r=0.999 9),0.392-6.272 μg/ml for paeoniflorin(r=0.999 7), 0.102-1.632 μg/ml for ferulic acid(r=0.999 7),1.326-21.216 μg/ml for berberine hydrochloride(r=0.999 8) and 1.95-31.2 μg/ml for baicalin(r=0.999 9),respectively;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 99.58%-102.47%(RSD=0.93%,n=9),99.21%-102.18%(RSD=0.90%,n=9),98.28%-101.23%(RSD=0.86%,n=9), 99.66%-101.84%(RSD=0.82%,n=9)and 99.18%-101.05%(RSD=0.62%,n=9). CONCLUSIONS:The method is accurate,re-liable,simple and fast,and can be used for the simultaneous determination of chlorogenic acid,paeoniflorin,ferulic acid,berber-ine hydrochloride and baicalin Lianqiao baidu pill.

Objective To investigate the clinical significance of tumor necrosis factor-?(TNF-?)and interleukin-6(IL-6)in the ascitic fluid of cirrhotic patients with spontaneous bacterial peritonitis(SBP).Methods TNF-? and IL-6 in the ascitic fluid of 32 cirrhotic patients with SBP were measured by ELISA.The group was compared with the value in transudatory ascites of 30 cirrhotic patients.Results The levels of TNF-? and IL-6 in the ascitic fluid of cirrhotic patients with SBP were much higher than those in transudatory ascites of cirrhotic patients(P

Objective To investigate the degree of vascular damage in patients with hypertension in various stages with transcranial Doppler (TCD), so as to provide references for the prevention and treatment of obstructive cerebral vascular disease. Methods TCD was adopted to examine bilateral anterior cerebral arteries (ACA), terminal internal carotid arteries (TICA), vertebral arteries (VA) and basal arteries (BA) in patients with hypertension (n=176) and normotensive individuals as control group (n=60). The hemodynamic data of two groups were analyzed statistically. Results In patients with stage Ⅰ hypertension, the blood stream velocity was increased in all intracranial vessels, especially in MCM and TICA. In patients with stage Ⅱ and Ⅲ hypertension, the elevation of blood pressure was followed by the decrease of blood stream velocity, especially in MCA, TICA and BA. The rate of abnormality as detected by TCD was 90 and 96 in patients with stage Ⅱ and Ⅲof hypertension, respectively. Conclusions The abnormal findings of cerebral vessels as detected by TCD seem to coincide with the clinical characters of stroke. TCD findings may be used as an early forewarning index of obstructive cerebral disease.

OBJECTIVE:To study the relation of glycyrrhizic glycoside on hepatitis B virus(HBV)replication in vitro.METHODS:2.2.15cell line was used and treated with glycyrrhizic glycoside,the contents of HBsAg and HBeAg in the su?pernatant of cell culture with different concentrations of glycyrrhizic glycoside were quantified.RESULTS:The inhibitory rates of glycyrrhizic glycoside on HBsAg were83.8%,71.0%,62.1%,52.0%and29.7%respectively at the concentrations of800,400,200,100and50?g/ml respectively.CONCLUSION:Glycyrrhizic glycoside does not promote HBV replication in vitro experiment.

Objective To investigate the drug resistance situation and the epidemiological characteristicin the tuberculoupatientin ordeto provide the reference foeffective prevention and treatmenof T.MethodTotally 2 271 patientwith posi-tive sputum smeapulmonary tuberculosi(TB) in the Jiangbei Branch Hospital of Chongqing Zhongshan Hospital from January 2009 to Decembe2013 were performed the drug resistance detection of 10 kindof anti-Tdrugs(isoniazid ,streptomycin ,rifampi-cin ,ethambutol ,para-aminosailcyliacid ,kanamycin ,pasiniazid ,protionamide ,capreomycin ,and ofloxacin) .ResultOf all 2 271 ca-seof positive sputum culture pulmonary T,the total drug resistance rate and total multi-drug resistance rate were 61 .91% and 26 .29% ,respectively ;the total drug resistance ratein initial treatmenand retreatmenpatientwere 52 .85% and 78 .06% respec-tively ,and the multi-drug resistance ratewere 16 .70% and 43 .38% ,respectively ;the top five in the average drug resistance ratefrom high to low were streptomycin(39 .45% ) ,isoniazid(33 .20% ) ,rifampicin(32 .94% ) ,para-aminosailcyliacid(31 .40% ) and pasiniazid(21 .53% );the anti-Ttreatmenhistory ,gende,age and occupation were significantly associated with the drug resist-ance occurrence (P＜0 .05) .Conclusion The drug resistance situation of the Tpatientin the local areof Chongqing iseriouin the recen5 year.The standardized managemenand treatmenon the Tpatientwith drug resistance should be strengthened .

Objective To explore the immune characteristic of a patient with hypogammaglobulinaemia and low natural killer(NK)cell numbers. Methods Case histories were analyzed repeatedly to grasp important characteristics of the disease. A routine blood examination and peripheral blood immunological detection was done. Complete neutrophil function test,lymphocyte proliferation ability and cytokine profile were evaluated. Results A 3-year-old boy was referred to the hospital because of fever and abdominal pain as well as partial intestinal obstruction. Laboratory examination revealed low immunoglobulin,reduced absolute numbers of B and NK cells and deficient B cell function of lower IFN-γ and IL-12 production. The patient also had mesenteric lymphadenopathy and a caecal mass with histology suggestive of ileocecal junctional tuberculosis. The patient was infected with cytomegalovirus(CMV)and fungus,which are not common in simple antibody deficiencies. The patient was followed for four years and during this period the patient had recurrent periodontitis resulting in the loss of one tooth. Conclusions Although the precise mechanism of the defect is unclear,we speculate that the case may cast new light on primary immunodeficiencies predominantly with antibody deficiency.(J Clin Pediatr,2011,29(7):601-608)

Objective: To investigate the mortality and probability of premature death due to cardio-cerebrovascular diseases, malignant tumors, diabetes and chronic respiratory diseases and their trends among residents at ages of 30 to 69 years in Xiacheng District, Hangzhou City from 2005 to 2020, so as to provide insights into the development of the chronic disease control strategy. Methods: The data regarding the mortality of cardio-cerebrovascular diseases, malignant tumors, diabetes and chronic respiratory diseases among residents at ages of 30 to 69 years in Xiacheng District, Hangzhou City from 2005 to 2020 were captured from the Zhejiang Provincial Chronic Diseases Surveillance Information Management System, and the crude mortality, standardized mortality by the sixth population census data in Zhejiang Province in 2010 and probability of premature death for these four chronic diseases were estimated. The trends in mortality and probability of premature death were analyzed in Hangzhou City from 2005 to 2020 using average annual percent change (AAPC). Results: A total of 6 436 deaths occurred due to cardio-cerebrovascular diseases, malignant tumors, diabetes and chronic respiratory diseases among residents at ages of 30 to 69 years in Xiacheng District, Hangzhou City from 2005 to 2020. The crude and standardized mortality rates were 183.33/105 and 152.88/105, which both appeared a tendency towards a decline from 2005 to 2020 (AAPC=-1.099% and -2.926%, both P<0.05), and the probability of premature death reduced from 10.40% in 2005 to 6.78% in 2020, which appeared a tendency towards a decline (AAPC=-2.827%, P<0.05). The probability of premature death due to malignant tumors, diabetes and cardio-cerebrovascular diseases all appeared a tendency towards a decline from 2005 to 2020 (AAPC=-2.681%, -6.571% and -2.286%, all P<0.05), while no significant changing trend was seen in the probability of premature death due to chronic respiratory diseases (AAPC=-4.263%, P>0.05). The probability of premature death for these four chronic diseases reduced from 13.55% in 2005 to 9.36% in 2020 among male residents (AAPC=-2.150%, P<0.05), and reduced from 7.18% in 2005 to 4.22% in 2020 among female residents (AAPC=-4.149%, P<0.05). Conclusions The mortality and probability of premature death due to chronic diseases appeared a tendency towards a decline in Xiacheng District, Hangzhou City from 2005 to 2020, and there was a significant decline in the probability of premature death for malignant tumors, cardio-cerebrovascular diseases and diabetes.