OBJECTIVE: To observe the therapeutic effect of fallopian incubation recanalization (FIR) and Sargentodoxa cuneata decoction coloclysis in the treatment of tubal obstruction infertility. METHODS: 180 patients with tubal obstruction infertility were randomly divided into therapy group and control group (n=90). Therapy group were given S. cuneata decoction after FTR via self-made semi-Y-shaped catheter; control group were given FTR only. RESULTS: Two years after operation, the total effective rate of therapy group was 83.3%. In control group,the total effective rate was 60.0%. There was a significant difference between two groups (P

Objective:To observe clinical therapeutic effect of Xiaoshuan Yizhi Capsules on multiple infarctional dementia(MID).Methods:50 cases of MID were randomly divided into Xiaoshuan Yizhi Capsules treatment group and Hydergine control group.Their symptoms and signs,cumulative scores of Haseguwa's Dementia Scale(HDS)and Mini-mental state examination(MMSE),blood lipids,hemorheologic indexes,superoxide dismutase(SOD)activity in red cells,plasma lipids of peroxide(LPO)content were investigated. Results:The total effective rate was 90% in the treatment group,and Xiaoshuan Yizhi Capsules not only improved symptoms and signes,increased cumulative scores of HDS and MMSE,and also regulated blood lipids,decreased hemorheologic indexes,raised SOD activity and decreased LPO content.Conclusion:Xiaoshuan Yizhi Capsule is superior to Hydergine in treatment of MID.

Objective To compare three methods for detection of antibody level in serum immunized with SARS-CoV-2mRNA vaccine. Methods Enzyme-linked immunosorbent assay(ELISA),pseudo virus-based neutralization assay(PBNA)and micro-cytopathic effect neutralization test(MCPENT)were used to detect the antibody levels of a total of 120 serum samples(40 before immunization and 80 after immunization)before and after 2 doses of mRNA vaccine immunization,and the consistency and correlation of the three methods were analyzed. Results The consistency rates of the three methods detecting 120 serum samples were all over 90%,the Kappa coefficients were all more than 0. 7,and each P was less than0. 01. The correlation coefficient(r)between the antibody potency results of positive serum samples detected by the three methods was 0. 825～0. 902,and each P was less than 0. 01. Conclusion The three methods have good consistency and correlation in detecting antibody level of serum immunized with SARS-CoV-2 mRNA vaccine.

Objective To establish and verify a stable pseudovirus-based neutralization assay(PBNA) for the detection of the potency in vivo of SARS-CoV-2 mRNA vaccine so as to use the method for the quality control of SARS-CoV-2 mRNA vaccines.Methods Three different immunization schedules of BALB/c mice(half male and half female) were set up, and each immunization schedule had two doses of vaccine. The titer of neutralizing antibody(Nab) in serum was detected by PBNA, and the appropriate immunization schedule was screened. After the immunization schedule was determined, the mice were immunized at the dose in the range of 0. 5-6 μg/mouse to further determine the linear range between serum Nab titer and immunization dose. The PBNA method for detecting the potency of SARS-CoV-2 mRNA vaccine in vivo was established,and the relative accuracy, intermediate precision, linearity and range of the method were verified. In addition, the potency in vivo of three batches of Omicron XBB.1.5 variant SARS-CoV-2 mRNA vaccine was detected by using the established method,and the results were compared with those of the original detection method of the enterprise.Results The immunization schedule of SARS-CoV-2 mRNA vaccine was determined as follows: the blood samples were taken 7 d after the booster immunization which was conducted 14 d after the primary immunization, the immune dose was 2 μg/mouse, with the linear dose range of 0. 5-3 μg/mouse. A PBNA method for detecting the potency in vivo of SARS-CoV-2 mRNA vaccine in mice was established. The results of methodological verification showed that the relative bias(RB) of each titer level detected by the method was within ± 20%, meeting the requirements of relative accuracy. The geometric coefficient of variation(GCV) of each titer level measured six times was less than 50%, meeting the intermediate precision requirements. The linear regression equation was fitted as y = 1. 003 4 x-0. 011 7, r = 0. 887 0, and the linear regression was significant(P = 9. 67 × 10~(-15)),in accord with the linear acceptability criteria. The titer level ranged from 25% to 150%, which met the quality standard range of in vivo potency of common vaccines. The method was used to detect three batches of Omicron XBB.1.5 variant SARS-CoV-2 mRNA vaccine, and the results were consistent with those of the original method, indicating that the method could be used for in vivo potency detection.Conclusion A stable PBNA method was established to detect the potency of SARS-CoV-2 mRNA vaccine in vivo, and can be applied to the quality control of SARS-CoV-2 mRNA vaccines.

Objective To study autonomic nerve regulator character and arrhythmias in the patients with essential hypertension(EH) and coronary heart disease(CHD). Methods The heart rate variability(HRV) and arrhythmias in 50 EH, 44 CHD, 52 EH + CHD patients and 35 healeh elderly subjects were investigated. Results There were significant differences of HRV in the patients with EH, CHD and healthy elderly subjects(P＜0.05).There was much significant difference of HRV between the patients with EH + CHD and healthy elderly subjects(P＜0.01), but no significant difference of HRV between the patients with EH and CHD(P＞0.05). The incidence rate of the premature atrial contraction, premature ventriculax contraction and atrial tachycardia in EH, CHD, EH+CHD and healthy elderly subjects, there were much significant differences( P＜0.01 ). Except the incidence rate of premature atrial contraction there was significant difference between the patients in CHD and EH + CHD (P＜0.05) ,and no significant differences of the incidence rate of other kinds of arrhythmias in the patients with EH,CHD and EH + CHD(P＞0.05 ). Conclusion The autonomic never function of EH and CHD patients was damaged, and the incidence rate of arrhythmias was increased(the premature atrial contraction often happened, then the premature ventricular contraction), and arrhymias happened in EH + CHD patients is most oftenly. The vagal hypoacfivity and sympathetic hyperactivity were major causes of reduction of HRV and probable causes in the elderly patients.

Objective To evaluate the value of plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP),C-reactive protein(CRP) and D-dimer(D-D) in prognosis of elderly patients confined to bed.Methods The clinical data of 150 elderly patients confined to bed were reviewed retrospectively,and 35 patients were in dead group and 115 patients were in survival group.The level o f plasma NT-proBNP,CRP and D-D were measured before treatment,during treatment and after treatment.Their characteristics and dynamic change were analyzed,and the value of NT-proBNP,CRP and D-D was evaluated in prognosis of elderly patients confined to bed.Results The level of NT-proBNP,D-D and CRP in dead group before treatment,during treatment and after treatment were significantly higher than those in survival group (P ＜ 0.05 or ＜ 0.01).The peak level of NT-proBNP,D-D and CRP in dead group emerged after treatment.By the way,the peak level of NT-proBNP,D-D and CRP in survival group emerged before treatment.Incidence of two or three markers positive in dead group [37.1％ (13/35),54.3％ (19/35)] was significantly greater than that in survival group [20.9％ (24/115),8.7％ (10/115)] (P =0.032,0.008).Multivariate Logistic regression analysis showed that the level of NT-proBNP,D-D and CRP were independent predictors of 28-day mortality (P ＜0.05).Conclusions The level of NT-proBNP,CRP and D-D are increased in severe elderly hospitalized patients.The level of above indexes have a dynamic change and reach peak before death.They are the independent prognostic markers of hospital mortality.Combined detection of NT-proBNP,D-D and CRP can improve the predictive value of hospital mortality in elderly patients confined to bed.

Objective To study the safety of Plumula nelumbinis microcapsules and its anti-arrhythmic effect.Methods Acute toxicity test in mice,arrhythmia mice models induced by chloroform and arrhythmia rats models induced by barium chloride(BaCl2)were adopted.Results The maximum tolerance dose for mice was 16.0 g/kg body weight.The microcapsule decreased the incidence of chloroform-induced ventricular fibrillation in mice significantly(P 0.05,compared with the blank control group).Conclusion The preliminary results show that Plumula nelumbinis microcapsule is safe,and exerts anti-arrhythmic effect against experimental arrhythmia.

The neonatal period is important for the dramatic increment as well as function improvement of islet β-cell.β-cell mass augmentation is primarily regulated by neogenesis,replication,and apoptosis.The mechanisms involve cell cycle related proteins and mitosis-promoting signaling pathways.This remodeling period has far-reaching effects on the body in adapting to the metabolic needs and regulating glucose homeostasis later in life.

Objective To examine the effect of resveratrol on the expression of monocyte chemoattractant protein-1(MCP-1)and brain natriuretic peptide(BNP)in myocardial cells of rats with acute pulmonary embolism.Methods Ninety-six SD rats were randomly divided into 2 groups:thromboembolism group (group T)and resveratrol + thromboembolism group (group TR),each group having 3 subgroups(n=1 6 each)in terms of different time points(1 h,4 h,8 h).The model of acute pulmonary embolism was established by infusion of autologous thrombi via the catheter inserted from the right jugular vein to the pulmo-nary artery.In group TR,resveratrol at 10 mg/kg (dissolved in 1%DMSO solution)was injected to the rats via the tail vein.Animals were sacrificed at 1,4 and 8 h after the model establishment.The rat hearts were harvested,fixed in 10%paraformaldehyde and paraffin-embedded.The expression levels of MCP-1 and BNP were detected by immunohistochemistry (SP method).Results Both MCP-1 and BNP were expressed in myocardial cells in group T and TR and gradually increased with time.The expression levels of MCP-1 and BNP were much lower in group TR than in group T at 4 and 8 h,with difference being statistically significant(P<0.05).Conclusion The expression of MCP-1 and BNP tended to increase with time in both groups.Resveratrol may protect the heart tissues by suppressing the expression of MCP-1 in myocardial tissues of rats with a-cute pulmonary embolism.