ObjectiveTo investigate the effect of immunological and inflammatory reactions on type 2 diabetic macrovascular disease.Methods120 patients with type 2 diabetes mellitus and 60 health controls were enrolled in this study.The patients were divided into two sub - groups,type 2 diabetes mellitus group and type 2 diabetic macrovascular disease group.The markers of immunological and inflammatory reactions were detected,and the main associated factors were collected and analyzed by one -way ANOVA and logistic regression.ResultsThe level of FBG,PPG,HbA1c,Hs-CRP and TNF-αt [ (9.86 ± 1.79)mmol/L,( 14.45 ±5.48) mmol/L,( 11.43 ±3.25) ％,(6.79 ±3.71 )mg/L,( 1.99 ±0.65) ng/ml] in the group of type 2 diabetic macrovascular disease group were higher than type 2 diabetes mellitus group [ (7,25±0.64)mmol/L,(10.45 t2.89) mmol/L,(8.56 ±1.58)％,(4.72 ±2.39) ag/L,(1.24 ±0.44) ng/ml,P ＜ 0.05 ].The risk factors in type 2 diabetic macrovascular disease were TNF-α,Hs-CRP and HbA1 c.ConclusionsImmunological and inflammatory reactions play a key role in type 2 diabetic macrovascular disease.

In observing the therapeutic efficacy of insulin sensitizer in treatment of early T2DM,120 T2DM patients were randomly divided into 3 groups: group A received 4 mg rosiglitazone daily;group B received 1.5 g metformin daily;group C received 4 mg rosiglitazone and 1.0 g met-formin every day.Before and after the treatment,fasting glucose,HbA1c,weight,adiponectin,BMI and HOMA-IR were measured or calculated.We found that fasting glucose,HbA1c and HOMA-IR of 3 groups were obviously decreased in comparison with their baseline and adiponectin was significantly increased.In conclusion,insulin sensitizer can obviously decrease the levels of glucose,HbA1c and HOMA-IR,and increase the level of serum adiponectin in T2DM.Insulin sensitizer in combination of metformin is suitable for treatment of T2DM.

ObjectiveTo investigate the influential factors of delayed diagnosis in the patients with cerebral palsy.Method50 cases with cerebral palsy were divided into 2 groups: beyond-12-months-group(20 cases) and under-12-month-group(30 cases). Their social facters were investigated and analyzed. SPSS statistical software was used.ResultThere is significantly difference between diagnosing group of under 12 months and that of beyond 12 months(the group of delayed diagnosis) in the factors of parents' educational level, the region where patients came from(urban or rural) and the type of cerebral palsy(P<0.01), While there is no difference in high risk factors(P>0.05). ConclusionIt is necessary for reducing delayed diagnosis to raise national cultural level, to eliminate difference between town and country, to spread the knowledge of early diagnosis and evaluation in cerebral palsy, to improve diagnosing ability in local hospitals, and to establish high risk newborns' long-term monitor system.

Objective To investigate the influence of a combination of Chinese herbs,Astragalus membranaceus var.mongholicus and A ngelica sinensis (A&A)on the production and elimination of reactive oxygen species (ROS)and the underlying mechanism during the process of renal interstitial fibrosis in the obstructive kidneys of rats with unilateral ureteral obstruction(uuo).Method Male Wistar rats were randomly divided into control,sham,UUO and UAA (UUO+A&A)groups.The rats in UAA group were administered with A&A(14 g/kg)by oral gavage once daily:the ones in sham and UUO group were given with equal volumes of water.Three days after setting up models,pathological injury of renal tissue was evaluated.Level of total antioxidant capacity(T-AOC)and activity of CuZn superoxide dismutase(CuZn-SOD)in renal homogenates were measured bv spectrophotometry.Expression of NADPH oxidase subunits p47-phox,p22-phox and nintrotyrosine was analyzed by Western blot. Results Severe interstitial inflammatory cells infiltration, mild tubular atrophy and interstitial fibrosis were found in UUO group, which were alleviated by A&A administration. Compared to sham group, T-AOC of UUO group was not significantly changed, but the expression of the nitrotyrosine, NADPH oxidase subunits p47-phox and p22-phox was increased significantly (P<0.05). After A&A administration, T-AOC level was increased (2.5±1.1 vs 1.5±0.5, P<0.05) and the expression of nitrotyrosine was decreased (P< 0.05) in UAA group compared with UUO group. Additionally, the expression of p47-phox was reduced at day 3 (P<0.05), accompanied with a reduced expression of p22-phox (P< 0.05). CuZn-SOD activity was not significantly changed among the groups. Conclusion The inhibition of A&A in NADPH oxidase subunits p47-phox and p22-phox, which is responsible for reduction of oxidative stress, is associated with the alleviation of renal fibrosis in obstructive rat kidney.

Objective:To evaluate the effects of intermittent change the angle of operating table on the skin in pressure areas of patients in prone position.Methods:A total of 150 hospitalized patients for elective surgical treatment in Yantai Yuhuangding Hospital from January 2019 to June 2019 were selected as subjects. The patients were divided into control group, 15° test group and 30° test group by random number table method, with 50 patients in each group. The control group received routine intraoperative pressure ulcer prevention measures, the 15° test group on the control group, the itinerant nurses adjusted the angle of the operating table according to the left 15°-supine-right 15° every half hour, and the 30° test group on the control group, the itinerant nurses adjusted the angle of the operating table according to the left 30°-supine-right 30° every half hour. The incidence of surgically related pressure ulcers and doctors' satisfaction with the surgical position of the three groups of patients were evaluated.Results:Pressure ulcers occurred in 8 patients of the control group, the incidence of pressure ulcers was 16%. Pressure ulcer occurred in 0 patient of the 15° test group, the incidence of pressure ulcers was 0. Pressure ulcers occurred in 2 cases in the 30° test group, the incidence of pressure ulcers was 4%. The difference was statistically significant ( χ2 value was 10.120, P<0.05). Further comparison showed that the incidence of pressure ulcers was statistically significant between the control group and the 15° test group ( P<0.016 7), while the incidence of pressure ulcers was not statistically significant between the control group and the 30° test group, between the 15° test group and the 30° test group ( P>0.016 7). The results of the postoperative doctors' satisfaction with the surgical body position were (27.880 ± 1.637), (27.520 ± 1.693), (26.920 ± 1.947) points in the control group, the 15° test group and the 30° test group, there was a statistically significant difference among the three groups ( F value was 3.779, P<0.05). Further comparison showed that there was no statistically significant difference in the score of surgical position satisfaction between the control group and the 15° test group, between the 15° test group and the 30° test group ( P>0.05). The satisfaction score of surgeons in the control group was higher than that in the 30° test group, and the difference was statistically significant( P<0.05). Conclusions:Intermittent adjustment of surgical position can reduce the incidence of pressure ulcers and improve the quality of nursing in the operating room without affecting the convenience of the surgeon. The optimal effect was achieved by adjusting the angle of the operating bed according to the left-leaning 15°-supine-right-leaning 15°at an interval of 30 minutes.

Objective: To investigate if bFGF can penetrate placental barrier. Methods: Sixteen day pregnant Wistar rats were selected. bFGF labeled with 125 I was injected peritoneally into the rats. The radioactivity of bFGF in different organs were determined in 30 min. Results: (1) 125 I bFGF was detected in the brain, heart, liver,lung and spleen. (2)With the same dose of 125 I bFGF, the concentration of it in the brain was at lowest level of all other organs.(3) In the range of safe dose, the permeability of bFGF through placental barrier was increased obviously. Conclusion: bFGF may penetrate placental barrier into rat's brain, which makes possible for the therapeutic intervention of bFGF in feotus. [

Objective To investigate the characteristics of chronic Keshan disease (CKD) and ischemic cardiomyopathy ( ICM ) and provide a basis for differential diagnosis . Methods Forty-two cases of CKD were randomly sampled from 6 Keshan disease districts in Sishui, Zoucheng, Juxian, Wulian, Yishui and Qingzhou Counties of Shandong Province, as CKD group. Thirty-one cases of ICM were selected from Qianfushan Hospital as ICM group. Echocardiography was used to analyze the cardiac morphology , hemodynamic and function. Results Left atrial internal diameter(LA), left ventricular(LV), end-diastolic internal diameter(LVd), right ventricular diameter(RV) and right atrial transverse diameter(RAtd) of the CKD group[(45.14 ± 6.93),(68.48 ± 6.24),(28.90 ± 3.14),(52.79 ± 6.62)mm] were higher than those of ICM group[(40.68 ± 5.12),(60.55 ± 4.07),(24.35 ± 2.12), (47.68 ± 8.53)mm , t = 3.03, 6.55, 7.38, 2.88, all P < 0.01]. In CKD group, several atrioventricular carities were enlarged, and the ventricular wall tended to become thin. In ICM group, left atrioventricular cavities were enlarged, and the apex of LV was thin spherical. The LV ejection fraction(LVEF) and fractional shortening of LV (LVFS) of the CKD group [(40.50 ± 10.68)%, (22.81 ± 6.24)%] were lower than those of the ICM group [(54.61 ± 6.58)%, (30.71 ± 4.29)%, t = - 6.957, - 5.976, all P < 0.01]. Diffuse hypokinetic motion of LV reduced in 85.7%(36/42) of CKD patients, and 83.9%(26/31) of ICM patients had segmental LV dyskinesia. Slight regurgitation of single valve was common in ICM group , but several regurgitation of multiple valves were more common in CKD group. Conclusion Echocardiography is a valuable noninvasive technology for differentiating CKD and ICM.

BACKGROUND: The integrity of the dura mater is very important for prognosis of patients with brain injury prognosis. Artificial dura mater is a commonly repair material, and to look for an ideal artificial dura mater is the exploration direction in the neurosurgery field. OBJECTIVE: To observe and analyze the clinical data of brain injury patients undergoing repair of colagen sponge as dura mater substitute material with ozone therapy, and to explore and evaluate its clinical value. METHODS: Folow-up results of therapeutic efficacy and complications in 60 cases of brain injury folowing repair with colagen sponge artificial dura mater and ozone treatment were retrospectively analyzed. RESULTS AND CONCLUSION:Two patients died of postoperative diffuse brain sweling, one died of brain injury with multiple organ failures, and two cases of extensive brain injury accompanied by cerebral herniation were in vegetative state. The rest 55 patients were enroled in the final analysis. After surgery, two patients appeared to have postoperative subcutaneous fluid and their conditions improved folowing puncture aspiration and pressure dressing with elastic bandage; another patient showed a smal amount of subdural effusion, but did not undergo special treatment, and dynamic head CT showed the effusion was gradualy reduced. Cranial CT examination showed no abnormalities associated with the artificial dura mater. At 3-6 months after surgery, the artificial dura mater was fused wel with the normal dura mater in 28 cases undergoing skul patching, and there was no adhesion and inflammatory reaction. Taken together, the colagen sponge artificial dura mater with ozone can give ful play to the decompression treatment of traumatic brain injury, which can maintain the brain function, shorter operative time, result in fewer complications, and have good compatibility, and moreover, the artificial dura mater can be fused wel with the normal dura to protect the brain cortex, thereby providing favorable conditions for the latter skul repair.

High level of glucose could induce apoptosis in human umbilical vein endothelial cells. C peptide and insulin had different effects on high glucose induced endothelial cells apoptosis. The reasonable application of C peptide may be helpful in preventing and treating diabetic macrovascular diseases.

Objective To investigate the clinical effect of human neural stem cells transplantation on severe visual disability infants after cerebral palsy. Methods Cells obtained from the forebrain of an 11-week-old abortive fetus were cultured and expanded for 15 days, then injected into cerebral ventricle of 7 patients. Results Their vision of 4 patients improved, as well as changes of flash visual evoked potential and functional magnetic resonance imaging in a few days after transplantation. Conclusion Neural stem cells transplantation may benefit in some CP children with severe visual disability.