With the progress of technology in separation and identification, proteomics is now widely applied in biomedicine.Because of the powerful ability to research differential protein expression and posttranslational modifications between normal and pathological samples on large scale level, proteomics provides the possibilities to screen biomarkers for diagnosis, therapy or prognosis of diseases.

BACKGROUND: The neurological basis for learning and memory is the high plasticity of central nervous system, and special rehabilitative training is necessary in the process of the functional recombination of central nervous system.OBJECTIVE: To observe the capability of rehabilitative training in differentiating learning ability and memory maintaining of one-trial passive avoidance response, and its correlation with the kinetic properties of Nmethyl-D-aspartic acid (NMDA) receptor channel of contralateral hippocampal neuron in rats with cerebral infarction.DESIGN: A randomized control animal trial.SETTING: Department of Rehabilitative Medicine, Affiliated Hospital of Luzhou Medical College.MATERIALS: The experiment was carried out in the central laboratory of Luzhou Medical College between March 2000 and February 2002. Twentyfour male Wistar rats were randomly divided into three groups with 8 rats in each: free movement after cerebral infarction group (model group), rehabilitative training after cerebral infarction group (rehabilitation group) and normal group.METHODS: ① Model establishment: Rats in the model group and rehabilitation group were made into models of infarction of right middle cerebral artery, and those in the normal group were not given any treatment. ②Rehabilitative training: After 4 days, only the rats in the rehabilitation group were trained with rolling-cage exerciser, screen exerciser and balance exerciser for 4 weeks. ③ Learning and memory tests: The learning and memory performances of all the rats were tested at 35 days postoperatively. The Y-maze test was applied to mainly observe the needed number f training for the rats to reach 9/10 correct response (for running to dark arm), and the multi-functional conditioned reflex box to observe the stayed duration on the diving board (step-through latency). ④ The single channel current property of NMDA receptor in hippocampal neurons was recorded with cell adhesion after learning and memory tests.MAIN OUTCOME MEASURES: ① training times needed for the rats to grasp the structure of the maze in each group; ② step-through latency in each group; ③ single channel current property of NMDA receptor in each group.RESULTS: All the 24 rats were involved in the analysis of results. ① The training times needed for the rats to grasp the structure of the maze in the rehabilitation group and normal group were significantly different from that in the model group [(68.02±11.67), (57.62±10.31), (107.07±16.32) times, P ＜0.05], and there was no significant difference between the rehabilitation group and normal group (P＞0.05). ② The medians of step-through latency before shock in the rehabilitation group and normal group were significantly different from that in the model group (286.7 s, 298.4 s, 126.7 s, P ＞0.05), and there was no significant difference between the rehabilitation group and normal group (P＞0.05). ③ The 35 pS short open NMDA receptor channel mainly existed in the hippocampal neurons of rats in the rehabilitation group, the open proportion was 0.099±0.007. The duration and proportion of 20 pS short and long open and the 35 pS short open NMDA receptor channels in the hippocampal neurons of rats were not significantly different from those in the normal group (P＞0.05). In the model group, 20 pS open channel was mainly observed, and the durations of 20 pS short and long open channels were obviously shorter than those in the rehabilitation group, the open proportion of 35 pS short open channel was 0.036t0.04, which was lower than that in the rehabilitation group,but no 35 pS long open channel was observed.CONCLUSION: Rehabilitative training accelerates the recovery of the learning and memory abilities of rats with cerebral infarction by changing the NMDA receptor channel properties of contralateral hippocampal neurons.

OBJECTIVE: To summarize the cervical dermatoglyphics distribution in 229 participants, and to design dermatoglyphic incision for cervical lymph nodes dissection, including II , III, IV or/and V regions, in accordance with the requirement for cosmetology. METHOD: To keep the patient's head right forward when the number and locations of the dermatoglyphy were collected by macroscopic observation. Upper Cricoid-Region is defined as the region above the cricoids, and Cricoids-Collarbone-Region is referred to the region below the cricoids. The relationship among the distribution of cervical dermatoglyph, patients' age, gender, and body mass index were analyzed. According to the distribution of cervical dermatoglyph, when performing the regional cervical lymph nodes dissection in patients with laryngocarcinoma and thyroid cancer, cervical dermatoglyphy incision or parallel dermatoglyphy incision were designed, and the operation time, operative complications, and cosmetology effects after surgery were observed. RESULT: Distribution of cervical dermatoglyphics was statistically correlated to the age, gender, and body mass index (P < 0.05) of patients. The follow-up time were 12 months to 49 months, 19 months on average. Average operating time of unilateral lymph nodes dissection was (46 +/- 12) minutes. Patients undergone the designs of cervical dermatoglyphy incision or parallel dermatoglyphy incision, suffered neither skinflap necrosis nor accessory nerve injury. One patient had lymphatic fistula after surgery, and relieved by conservative treatment. One with T3 N2 M0 laryngocarcinoma got V region lymph nodes recurrence 6-months after surgery. CONCLUSION The cervical lymph nodes dissection with dermatoglyphy design is safe with prominent cosmetology effects. It will take a little longer time to perform the neck dissection with the dermatoglyphy incision in the beginning.
Adolescent ; Adult ; Aged ; Asian Continental Ancestry Group ; Child ; Child, Preschool ; Dermatoglyphics ; Female ; Humans ; Laryngeal Neoplasms ; surgery ; Male ; Middle Aged ; Neck ; anatomy & histology ; Neck Dissection ; methods ; Young Adult

Objective　To discuss the characteristics and mechanism of P3b potentials in rats and establish the animal model of P3b.Methods　P3b potentials and cell electric discharge in hippocampal CA1 region were recorded simultaneously inside cranio, septal and hippocampal CA1 region in rats.After septal lesions,the changes of P3b and cell electric discharge in hippocampal CA1 region were observed.Results　P3b in hippocampal CA1 region was correlated well with its cell electric discharge. Following septal lesions,P3b and cell electric discharge decreased gradually and disapeared eventually. Conclusion　Cell electric discharge in hippocampal CA1 region might be an important source of P3b.Normal structure and function of septal region were necessary to P3b's generation in hippocampal CA1 region. Additionally these suggested that cell electric discharge to target stimulation was important to P3b's generation.

Objective To evaluate the effects of Xin-ke-shu and Betaloc on arterial elasticity of coronary heart disease(CHD).Methods According to the results from coronary angiography,50 patients who were taking long-term treatment of coronary artery obstructions were enrolled and divided into two groups in equal number at random.They were divided into Xin-ke-shu group(n=25)and Betaloc group(n=25).The Xin-ke-shu group received Xin-ke-shu(12/d)individually and the Betaloc group received Betaloc(50mg/d)for eight weeks.Patient's heart rate was measured by the doctor.Brachial blood pressure was measured by standardized mercury cuff sphygmomanometer.Automatic pulse wave velocity(PWV)measurement system and Complior Colson device and DO-2020 were applied to examine the carotid-radial artery PWV(PWVcr)and the large arterial elastic indexes(C1 and C2)respectively.Results After eight weeks of treatment.All patient's heart rate showed significant decrease.The Betaloc group,after excluded the betaloc effect,showed significant decrease of blood pressure.The Xin-ke-shu group showed significant increase of C1 and C2,and PWVcr slowed down obviously.Conclusion Xin-ke-shu improves the small and large arterial elasticity,and attenuates the wave reflex amplitude.

Objective To observe the effect of neuropeptide Y(NPY) on the levels of serum interleukins(ILs)in guinea pigs with experimental allergic encephalomyelitis(EAE).Methods 30 guinea pigs were divided randomly into normol control group,EAE group and NPY group.The NPY was injected into the intracerebroventricular in NPY group one week before the EAE model be made.The EAE molders were made by injecting the homogenate of rat's spinal cord into the plantar of guinea pigs in groups EAE and NPY.Then,the neurological deficits score was observed everyday,the incidence and the delitescence of EAE were observed too.The levels of serum IL-1?,IL-2,IL-4,IL-6 and IL-8 were detected,and the neuropathological examination was used when symotoms peak period of EAE models.Results(1)The incidences of the EAE in groups EAE and NPY were 100% and 90%,the delitescences of EAE were(10.0?4.8)d and(25.4?12.6)d,the neurological deficits scores at the symotoms peak period were(3.60?0.52) and(1.80?1.14),respectively.There were significantly differences between the two groups(all P

Capillary zone electrophoresis (CZE) was used to monitor the whole unfolding process of bovine insulin resulted from the dithiothreitol (DTT) reduction. An off-line matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) was employed to determine the molecular weight of the reaction products at the same time to confirm the observation results obtained by CZE. The structural change during the process of bovine insulin unfolding could be observed directly from the electropherogram and information of protein unfolding could be obtained simultaneously. The results indicated that as an effective tool of monitoring the conformational change of protein,the method of CZE was simple,quick,sensitive and lower sample consumption.

We here systematically summarize our practice and its effectiveness in the creation and development of the discipline proteomics in Academy of Military Medical Sciences,and the establishment of a system for identifying and training talented researchers in proteomics.The methods we used to train the researchers and to develop a vigorous team are discussed.These practices contributed much to the development of proteomics in China.

Objective To evaluate the clinical efficiency and seeurity of SilverHawk directional atherectomy device in treating arteriosclerosis obliterans of lower extremity.Methods Seyen patients with lower extremity ischemia were treated with SilverHawk directional atherectomy.The lesions length varied from 1.8 to 14.5 cm.Clinical symptoms(Fontaine classification)included 4 cases of grade Ⅱ b,1 case of grade Ⅲ,2 cases of grade Ⅳ.The ABI varied from 0.28 to 0.65.Patency was evaluated with color duplex sonography or CTA besides clinical examination during follow-up.Results Seven occlusive lesions were recanalizated successfully.The technical success(residual stenosis<30%)rate was 100%. Clinical symptoms were obviously improved or disappeared.The ABI varied from 0.82 to 1.18(P=0.002),and Fontaine classification included 6 cases of Ⅰ:1 case of Ⅱ a.Patency rate was 100%and the ABI varied from 0.75 to 1.14(P=0.938).Fontaine classifications keep consistent l-6 nonths after operation.Conclusion SilverHawk drectional atherectomy Was a safe and effective way for treatment of lower arteriosclerosis obliteran.

Objective To study the cytotoxic activity of Chinese compound prescription (Xiaozheng Yiliu Decoction) combined with cisplatin on human ovarian cancer OVCAR/DDP cells and its molecular mechanism. Methods The xenograft model was established by injection of OVCAR/DDP cells. Forty nude mice were randomly allocated into four groupsincluding control group, cisplatin treatment group, low-dose Xiaozheng Yiliu plus cisplatin treatment group,and high-dose Xiaozheng Yiliu plus cisplatin treatment group.The apoptosis was determined by TUNEL analysis.The survivin expression was detected by RT-PCR analysis.Results The inhibit rates in cisplatin treatment group, low-dose Xiaozheng Yiliu plus cisplatin treatment group,and high-dose Xiaozheng Yiliu plus cisplatin treatment group,were(5.56±1.14) ％,(18.42±7.73) ％,and (31.59±12.58) ％,respectively,and there were significant differences among the groups(F=8.64,P＜0.01 ).The TUNEL analysis showed that the percentages of cell apoptosis were(2.35±1.04) ％,(4.56±1.61) ％,(12.42±3.68) ％,(21.59±5.17) ％ in four different groups,respectively.After treated by Xiaozheng Yiliu Decoction,the mRNA expression of survivin was found to be down-regulated by RT-PCR analysis. Conclusion Xiaozheng Yiliu Decoction could iucrease the cytotoxic activity of cisplatin on ovarian cancer OVCAR/DDP cells, which may be related to the down-regulation of survivin gene expression.