Objective To investigate the relationship between serum visfatin and glucose metabolism disorder in patients with polycystic ovary syndrome.Methods Seventy eight patients with polycystic ovary syndrome (PCOS) were selected,and,according to the results of glucose tolerance test and insulin release test (OGTT),were divided into normal OGTT without insulin resistance (NONIR) group (n =32 cases),OGTT normal with hyper insulinemia (HI) group (n =25 cases),and OGTT abnormalities that may be associated with hyper insulinemia (also known as abnormal glucose metabolism (AGM) group; n =21 cases).Sixteen cases of tubal infertility women were selected as control group in the same period.The relationship between serum visfatin and glucose metabolism was analyzed and related to various factors.The changes of relevant indicators of PCOS patients who were treated before and after treatment were observed in AGM group.Results In AGM Group,PCOS patient's menstrual cycle,testosterone (T),luteinizing hormone (LH)/follicle stimulating hormone (FSH),visfatin,triglyceride (TG),homeostasis model assessment of insulin resistance (HOMAIR) were higher than control group (P ＜ 0.05).The age at menarche,menstrual cycles,infertility time,waist-hip ratio (WHR),systolic blood pressure,T,estradiol (E2),and LH/FSH were no significant difference between AGM group versus NONIR group or HI group (P ＞0.05).However,the body mass index (BMI),waist circumference,diastolic blood pressure,HOMA-IR,and TG in AGM group were significantly higher than NONIR group,and older than HI group; visfatin in AGM group was higher than NONIR group or HI group (P ＜ 0.05).Visfatin was positively correlated with BMI,waist circumference,fasting insulin (FINS),fasting plasma glucose (FPG),HOMA-IR,and TG (r =0.423 ～ 0.847,P =0.01 ～ 0.04).After 3 months of treatment,8 cases AGM patients' serum visfatin,HOMA-IR,and blood pressure were significantly decreased (P ＜ 0.05).Conclusions High serum visfatin may be one predictor of glucose metabolism in patients with PCOS.Especially,the patients with obesity should be highly vigilant glucose metabolism disorder occurring and should be given positive joint programs treatment for weight control.

Objective: To study the phenotype characteristic of mandibular condylar chondrocytes (MCCs) in vitro. Methods: MCCs of two-week-old New Zealand rabbits were isolated with enzyme digestion and cultured in DMEM. Phase contrast microscopy, image analysis system, MTT assay and immunocytochemistry method were used to compare MCCs of different passages in cellular shape and size, growth curve and expression of type I and type II collagen. Results: The majority of MCCs in earlier passages (1～3 passage) were polygonal, while more fusiform and spindle-shaped cells were found after 5～6 passages. MCCs in earlier passages were smaller than those in sixth passages. The proliferation rate of MCCs decreased with passaging. There were more type II collagen positive cells in 1 ～ 3 passages, while more type I collagen positive cells in 7th passage. Conclusion: The changing characters of MCCs in vitro such as cellular shape and size and expression of type I and II collagen are similar to those of MCCs in vivo from proliferating zone to hypertrophic zone. MCCs in later passages may be with dedifferentiation.

Objective To elucidate the therapeutic mechanism of 1,25-dihydroxyvitamin D3 and its analogues in psoriasis. Methods The modulating role of calcitriol, calcipotriol and tacalcitol on the expression and secretion of IL-10 in monocyte-derived dendritic cells (MDDCs) was investigated. Human monocytes were differentiated into MDDCs in vitro in the presence of GM-CSF and IL-4. Semi-quantitative RT-PCR and ELISA were used to analyze the mRNA expression and protein production of IL-10 in MDDCs. In each experi-ment, 24,25-dihydroxyvitamin D3 (a vitamin D3 metabolite with a low affinity to the vitamin D receptor) was used as a control. Results The mRNA expression and protein secretion of IL-10 in MDDCs was up-regulated by calcitriol, calcipotriol or tacalcitol with a similar potency, but not by 24,25-dihydroxyvitamin D3. The up-regulatory role of calcitriol on IL-10 protein secretion was dose dependent. Conclusion The therapeutic effect of calcitriol and its analogues in the treatment of psoriasis may be related to the induction of IL-10 production.

On the basis of generally stereotactic radiotherapy technology,intensity modulated radiation therapy technology has been developed greatly since 1990's.This technology is to improve the radiation dose distribution in the target so that the high dose area is more conformal for the three-dimentional shape of the target and the radiation dose in the normal organs and tissue around the target decreases.The characteristic of this new technology is discussed systematically in this paper.

Nicotinamide and Exendin-4 could promote transdifferentiation of rat BM-MSCs into insulin-producing cells in vitro, which expressed insulin, C-peptide and related genes.

The medical records of 197 patients on linezolid therapy were collected retrospectively.The prevalence and risk factors for linezolid-induced reduction of hemoglobin were identified.The incidence of linezolid-induced reduction of hemoglobin was 31.98％.The patients with reduction of hemoglobin had older age [(68 ± 14) vs.(59 ± 18) years,P =0.000] and lower creatinine clearance (CCr) [(72 ±42)vs.(98 ± 52) ml/min,P =0.000].Multivariate logistic regression analysis showed that age ≥65 years,treatment duration ≥ 15 days,CCr ＜ 65 ml/min and pre-treatment hemoglobin value ＜ 95 g/L were significant risk factors for linezolid-induced reduction of hemoglobin.

Objective To investigate telomerase activity in patients with psoriasis. Methods The telomerase activity in the skin lesions of patients was detected by TRAP (Telomeric repeat amplification protocol)——ELISA which is based upon PCR amplification of the initial telomerase product and detected by ELISA. Results Telomerase activity was detected in the lesions of psoriatic patients, but the level of it was lower in comparison with that in tissues of malignant tumor and K562 cell line. Conclusion These findings support that the increase of telomerase activity may be linked to the pathogenesis of psoriasis and that the telomerase activity can be detected not only in malignant tumors but also in nonmalignant skin diseases.

Objective To investigate the clinical and histological characteristics of trichilemmal carcinoma (TLC). Methods A clinicopathological analysis of 13 cases of TLC was carried out. Results There were 9 males and 4 females among the 13 patients with TLC who were aged from 34 to 87 years (mean: 70 years). Clinically, the tumor presented as an exophytic mass; histologically, it was characterized by the proliferation of epithelial cells and keratinization of outer root sheath. Cytologically atypical clear cells predominated in the tumor tissue. Microscopy revealed different growth patterns of tumor cells, which included solid growth pattern, tobular pattern and trabecular pattern. Periodic acid Schiff (PAS) stain demonstrated clear cells in all the tumor tissues from the 13 patients. Immunohistochemistry was performed in tissue samples from 6 patients, and showed that these samples were positive to high molecular weight cytokeratin (CK-HMW) and epithelial membrane antigen (EMA), but negative to carcinomebrynic antigen (CEA), S-100, cytokeratin 8 (CK8)and epithelial antigen(Ber-Ep4). Follow-up over 4 months to 5 years revealed neither recurrence nor metastasis in 9 cases.Conclusions TLC is a low-grade malignancy of skin adnexal tumor without distinctive clinical features, and should be differentiated from other malignant clear cell tumors of the skin.

Objective To assess the histopathological characteristics of tumors arising in nevus sebaceous.Methods The clinical and pathological data on 16 patients with tumors arising in nevus sebaceous were collected and retrospectively reviewed.Histopathological characteristics were analyzed.Results Of the 16 patients,12 aged ≥ 18 years,4 aged 11 to 17 years; 7 had syringocystadenoma papilliferum(SCAP),4 tubular apocrine adenoma (TAA),3 trichilemmoma,2 basal cell carcinoma (BCC),1 porokeratotic eccrine ostial and dermal duct nevus,and 1 nevus flammeus.Six patients suffered from more than one kind of tumor,of these tumors,concurrent syringocystadenoma papilliferum and apocrine adenoma predominated and were observed in 4 of these patients.Conclusion Any tumor arising from nevus sebaceous should be closely followed up.

Objective To investigate the performance of surgical management in facial skin malignancies.Methods From January 2000 to December 2006,65 patients with facial skin malignancies,including47 cases of basal cell carcinoma.10 cases of squamous cell carcinoma,3 cases of dermatofibrosarocoma protuberans,2 cases of malignant melanomas,and one case of malignant acanthoma,hemangioendotheliosar-coma and sebaceous carcinoma,respectively,were collected and managed with wide resection followed by reconstruction.In order to achieve a thorough resection,frozen sections were prepared and subjected to pathological examination during the operation process to ensure the margins of resection were free of malignancy.Reconstruction was carried out by direct closure,or with local random flaps,extended flaps,free skin grafts.Resuits All defects were managed by one-stage reconstruction.The survival rate of skin flaps/grafts was 100%,and a satisfactory appearance and function was achieved.During the follow-up from 6 months to 5 years,local relapse was observed in one patient with basal cell carcinoma and one with squamous eell carcinoma,lymphatic metastasis in one with squamous cell carcinoma.Distant metastasis occurred in a patient with malignant melanoma.who died consequently.Conclusions Thorough resection is the key to prevent relapse of facial skin malignancies after surgery.Appropriate reconstruction may favor the restoration of facial appearance,and local random flaps appear to be the best reconstruction strategy.