Objective To analyze the correlation between the blood typing test,irregular antibodies screening of pregnant women and hemolytic disease of newborn(HDN).Methods The ABO blood type and Rh(D)blood type of pregnant women were detected,and the irregular antibodies of pregnant women were screened.The blood type serol-ogy and hemolytic disease were detected in neonates with jaundice.Results In specimens of 2 032 pregnant women with type O blood,their husbands were non -O blood type,after delivery,152 neonates were diagnosed as HDN. Among 2 032 pregnant women with type non -O blood,only 5 neonates were diagnosed as HDN.There was significant difference between the two groups (χ2 =135.4,P <0.01).27 pregnant women with RhD negative type,their hus-bands were RhD positive,after delivery,4 neonates were diagnosed as HDN.Conclusion Pregnant women's blood type and irregular antibody are closely related to neonatal hemolysis disease.So pregnant women's blood type and irregular antibody should be regular project in pregnancy,and so as to prevent neonatal hemolytic anemia caused by blood type of feto -maternal incompatibility.

The oocyte cryopreservation is more promising than embryo freezing in clinical applications,preservation of female fertility,legal and ethical aspects in this decade.The redundant oocytes retrievedfrom ⅣF cycles can potentially donate oocytes,which have unique advantages in economy and feasibili-ty.We have achieved success in embryo freezing,but just obtained poorer results in oocyte cryopreserva-tion which was mainly because of the low rates of survival,fertilization,and cleavage.The character ofthe plasma membrane and the time of cortical granules present at the metaphase of meiosis Ⅱ with thespindle system consist of the major difference between oocytes and embryos.Moreover,the oocytes shouldbe fertilized by sperm at the appropriate time.We used a refined slow freezing method to improve the sur-vival rate and increased sucrose concentration to dehydrate oocytes.Vitrification was another approach toprevent harms.Besides,intracytoplasmic sperm injection was used to overcome possible zona hardeningafter the release of cortical granules.Oocytes after cryopreservation showed serious disturbances of the mi-crotubules immediately after thawing as they were vulnerable to the thermal changes and easy to depoly-merize.Fertilization of these oocytes with disorganized spindles led to chromosomal aneuploidy,digyny,and degradation of the cleavage rate.We can improve cryopreserved oocytes to normal fertilization anddevelopment by appropriate incubation and timing of insemination,as the microtubules repolymerize in atime dependent way after incubation which is compatible with recovery of the spindles.With the improve-ment of survival,fertilization,and development,oocyte cryopreservation will play an imperative role.

College reading course occupies an important position in English acquisition.The introduction of integrated approach in college English reading course can effectively improve students’reading capability.This article analyses the importance of integrated approach in college English reading course in aspects of teacher,student,and reading techniques and presents some specific methods to help students read better.

Objective To explore the effects of oral vitamin D supplementation on insulin resistance(IR)in the patients with type 2 diabetes mellitus(T2DM ) .Methods A total of 16 elderly patients with T2DM in our hospital were included as the research subjects and divided into the observation and control groups according to the random number method .The two groups received the conventional hypoglycemic therapy .The observation group was simultaneously added with calcitriol (0 .50μg/d for 12 weeks) .The blood sugar related indicators and serum 25-(OH)D3 level were detected by collecting fasting venous blood before and after treat-ment measured .Results BMI ,FBG ,HbA1c ,Fins ,25-(OH)D3 and IRI after treatment in the two groups were improved signifi-cantly with statistically significant difference (P<0 .05) ,moreover BMI ,FBG ,HbA1c ,Fins ,25-(OH)D3 and IRI after treatment in the observation group were improved more significantly compared with the control group ,and the differences were statistically sig-nificant (P<0 .05) .The patients with 25-(OH)D3 level < 25 nmol/L had significant improvement in BMI ,FBG ,HbA1c ,Fins and 25-(OH)D3 level ,the differences were statistically significant (P<0 .05) .There was positive correlation between 25-(OH)D3 with BMI ,FBG ,HbA1c ,Fins and IRI (P<0 .05) .Conclusion The vitamin D supplementation can better improve the IR status on the basis of conventional hypoglycemic therapy and promotes the blood glucose stability in the elderly patients with T2DM .

Objective To investigate correlation between vitamin D and glucose metabolism,lipid metabolism, insulin resistance,early secretory function of pancreatic islets in postmenopausal women with type 2 diabetes. Methods 115 postmenopausal women with type 2 diabetes were selected as observation group.115 postmenopausal women without type 2 diabetes were selected in hospital at same period as control group.All patients received detection of vitamin D,glucose metabolism,lipid metabolism,insulin resistance,early secretory function of pancreatic islets. Correlation between vitamin D and glucose metabolism,lipid metabolism,insulin resistance,early secretory function of pancreatic islets were analyzed.Results Fasting blood glucose,postprandial 2h blood glucose,glycosylated hemoglobin, body mass index,total cholesterol,triglyceride,low density lipoprotein cholesterol,insulin resistance index in the observation group were higher than the control group [(8.5 ±1.2)mmol/L vs (5.1 ±0.6)mmol/L,(13.4 ± 1.5)mmol/L vs (7.8 ±0.7)mmol/L,(8.7 ±1.1)% vs (5.6 ±0.5)%,(25.7 ±1.0)kg/m2 vs (22.3 ±0.4)kg/m2 , (5.6 ±0.7)mmol/L vs (4.5 ±0.4)mmol/L,(2.3 ±0.5)mmol/L vs (1.7 ±0.3)mmol/L,(3.5 ±0.6)mmol/L vs (2.2 ±0.4)mmol/L,(3.6 ±0.5)vs (2.5 ±0.4),t =9.667,10.179,8.535,4.524,5.443,6.529,8.909,7.406, all P <0.05].High density lipoprotein cholesterol,insulin first secretion phase peak factor,pancreatic islet βcell function index,fasting insulin,vitamin D in the observation group were lower than the control group [(1.0 ± 0.2)mmol/L vs (1.2 ±0.3)mmol/L,(5.2 ±1.3)vs (8.4 ±1.6),(135.8 ±11.7)vs (194.3 ±19.2),(4.1 ± 0.9)IU /mL vs (10.8 ±0.6)IU /mL,(10.8 ±2.1)ng/L vs (27.4 ±3.8)ng/L,t =5.087,9.153,7.370,10.414, 18.371,all P <0.05].Vitamin D was significantly negatively correlated with fasting blood glucose,postprandial 2h blood glucose,glycosylated hemoglobin,body mass index,total cholesterol,triglyceride,low density lipoprotein choles-terol,insulin resistance index(r =-0.537,-0.512,-0.469,-0.387,-0.325,-0.316,-0.372,-0.457,all P <0.05).Vitamin D were significantly positively correlated with high density lipoprotein cholesterol,insulin first secretion phase peak factor,pancreatic islet βcell function index,fasting insulin(r =0.351,0.418,0.674,0.316,all P <0.05).Conclusion Vitamin D deficiency in postmenopausal women with type 2 diabetes may be involved in glucose -lipid metabolism and insulin resistance,but also may be one of the reasons leading to decreasing of early secretory function of pancreatic βcells.

Objective To investigate the mean values of carotid intima-media thickness (IMT) and change features according to gender,segments and age groups in general Chinese population,and provide the basis for the health assessment of carotid ultrasound.Methods GE LOGIQ 7 ultrasound scanner was used to measure IMT of 743 healthy cases aged from 38 to 70 years old.Six segments(bilateral common carotid artery,bilateral carotid bulb,bilateral internal carotid artery) were measured for each case.All patients were divided into 6 age groups across 5 years to be one group.The mean values for each group was calculated.Results The average age of the three sections of carotid IMT gradually increasing linearly related; minimal change in men and women,the magnitude of the age of the internal carotid artery IMT;sinus IMT were greater than the common carotid artery IMT and carotid artery IMT thinnest.the mean carotid IMT of Men and women in the same age group,compared to the 46-50 group,the group of 51-55,56-60 group,the differences were significant (P ＜ 0.05);men and women 41-45 group and 61-65 difference between the groups was not statistically significant (P ＞ 0.05);mean IMT difference of the male groups compared with the older group of women was not statistically significant (P ＞ 0.05).Conclusion Ultrasound scanner can be used to measure IMT accurately.In the 46-60 group,carotid IMT thickening in men than women about 5 years earlier.The mean IMTs for different sex,age groups and segments would greatly benefit vascular health evaluation.It has a great significance in the prevention of cardiovascular and cerebrovascular diseases.

Objective To study the expression and significance of excision repair cross complementing genes 1 (ERCCI) in the tissue of nasopharyngeal carcinoma(NPC).Methods ERCC 1 proteins was assayed with immunohistochemical method in the tissue of 103 cases of NPC and 50 cases of chronic inflammation of nasopharyngeal epithelium(CINE).Results The expression rate of ERCC1 was 45.63％ in NPCs,which was significantly lower than that in CINE (70.00％) (χ2 =8.037,P ＜ 0.05).The expression rate of ERCC 1 in NPCs had relationship with T,N staging and clinical staging as well as the presence of recurrence and metastasis (χ2 =9.007,9.992,8.343,5.792,all P ＜ 0.05),while no relationship with age and gender(χ2 =0.026,0.871,all P ＞ 0.05).The expression of ERCC1 in group of recurrence and metastasis was significantly lower than those in group of no recurrence and metastasis.Conclusion The expression of ERCC1 was significantly decreased in NPCs.ERCC1 will probably become prognostic biological parameters of NPC.

Objective To evaluate the effect of “T”incision on distant stereovision of cataract patients with high cornea astigma-tism .Methods 40 age-related cataract patients (40 eyes) with high cornea astigmatism for surgery in our hospital were divided into two groups :A(20 eyes) an B(20 eyes) .The A groups received superior(11 :00) clear corneal incision(CCI) .The B groups besides the superior(11:00) CCI ,for surgery on the steepest meridian ,an additional couple of “T”incisions were made to release the preex-isting corneal astigmatism .The operation eyes were all implanted Rayner foldable posterior chamber intraocular lens .The UCVA , corneal astigmatism ,distant stereovision were observed at 3 months after operation .Results The UCVA in each group after sur-gery was all better than that of preoperation ,difference being statistically significant (P<0 .01) .the difference between Group A and Group B was significant(P<0 .05);In Group A ,there were no significant difference on the corneal J0 and P before and after op-eration(P>0 .05) ,The corneal J0 and P in group B after surgery were lower than that of preoperation .difference being statistically significant(P<0 .01) .difference between Group A and Group B(P<0 .01) at 3 months postoperation was statistically significant (P<0 .01);The stereopsis in each group after surgery was all better than that of preoperation ,difference being statistically significant (P<0 .01) .the difference between Group A and Group B postoperation was significant (P<0 .05) .Conclusion the“T” incisions on the steepest corneal meridian can improve the patient′s visual function and distant stereovision .

Objective To observe the change of serum Omentin-1 in gestational diabetes mellitus(GDM)and to investigate its clinical significance.Methods Serum Omentin-1 level was detected in 85 cases of GDM and 85 matched cases of normal glucose tol-erance(NGT)by ELISA,the glycolipids biochemical indexes,inflammation indexes and fasting insulin (FINS)level were simultane-ous detected,then the insulin resistance index (HOMA-IR)was calculated in both groups.Results The levels of prenatal prepreg-nant BMI,FPG,hs-CRP,blood lipids,blood glucose,FINS and HOMA-IR in the GDM group were significantly higher than those in the NGT group,while serum Omentin-1 level was obviously lower than that in the NGT group,difference was statistically signifi-cant (P<0.05);when prenatal obesity and/or HOMA-IR≥2,the serum Omentin-1 levels was significantly decreased.Serum Omentin-1 level had significant positive correlation with HDL,and negative correlation with prepregnant BMI,prenatal BMI,FPG, FINS and HOMA-IR.The multiple stepwise regression analysis showed that prepregnant BMI,TG,FPG and FINS were independ-ent influencing factors of serum Omentin-1 in GDM.Conclusion Serum Omentin-1 is associated with GDM closely,which can re-flect the degrees of sugar,lipid metabolic disorder and insulin resistance in pregnant women,and may be involved in the occurrence and development of GDM.

OBJECTIVE:To optimize the water extracting technology of Rubingxiao tablet.METHODS: The extracting technology of Rubingxiao tablet was optimized by orthogonal experiment with the yield of extractum and ferulic acid content as indices. RESULTS: The optimum extracting condition was A3B1C3D2,i.e. adding 12 times water and extracting 0.5 h/time for 3 times with 60% alcohol. CONCLUSION: The extracting technology for Rubingxiao tablet is simple and rapid with stable result, and it provided theoretical basis for the production of Rubingxiao Tablets.