For the requirements of social development,the medical model has changed from the medical model of organism to the model that of society-mentality-organisms. Thus,pediatrician must change old ideas and old model of thinking,improve knowlege structure and reform medical education model.

Objective To determine the content of hesperidin in Weifukang capsules by HPLC,and to establish the quality standard of Weifukang.Methods HPLC method was performed on C18 column(4.6 ?200 mm,5 ?m).The mobile phase consisted of acetonitrile-sodium dihydrogen phosphate(0.05 mol/L)-phosphoric acid(200 ∶800 ∶0.5).The flow rate was 1.0 mL/min,column temperature was 25 ℃,and the detection wavelength was 283 nm.Results Hesperidin was linear at the range of 0.38 ?g～1.90 ?g(r=0.999 2)the mean recovery rate was 97.88 %,and RSD was 1.69 %.Conclusion This method is effective and reproducible and provides a basis for the quality control of Weifukang capsules.

Azathioprine(AZA) and 6-mercaptopurine(6-MP) have a well-established effect as an immunosuppressive agent for inflammatory bowel disease(IBD),Myelosuppression is the most serious side effect during treatment.Thiopurine methyltransferase(TPMT) plays an important role in the metabolism of 6-MP. This article reviews the literature on the role of TPMT measurement prior to treatment of IBD with AZA/6-MP.

Wnt gene family has been shown to encode the multifunctional signaling glycoproteins that are involved in the regulation of a wide variety of normal and pathologic processes,including embriogenesis,differentiation,and tumorigenesis.Recent studies have demonstrated that the Wnt-5a expression is frequently deregulated in various human cancers,which suggested that Wnt5a shuld be a good marker for progression and prognosis of tumor,but the role of Wnt-5a is controversial in different tumors.Here,we assemble evidence of possible relations between Wnt and various tumors.

Objective Established the model of atherosclerosis (AS) cells.To explore Hydrogen sulfide donor (NaHS) in atherosclerosis model by oxidized low density lipoprotein (ox-LDL) induced of the NF-κBp65 factor expression.MethodsApplication concentration of 80ug/mL ox-LDL induced liver cancer cell HepG2 cells AS model, set up a control group and treatment group (10μmol/L、15μmol/L、30μmol/L、100μmol/L、500μmol/L、1000μmol/L), to observe hydrogen sulfide this cell model of the cell proliferation activity and the determination of TNF-α and IL-10 factors of the expression, through the RT-qPCR and Western-blot method for determining the expression of the NF-κBp65 expression situation.ResultsAt 24h the ox-LDL induced a large number of red-stained granules, free cholesterol and total cholesterol in HepG2 cells increased, and free cholesterol content was more than 50% of total cholesterol in HepG2 cells, indicating that AS cell model was established successfully.CCK8 method of cell proliferation activity test results showed that the NaHS in 30umol/L, duration of 48 h when the largest cell proliferation activity.The TNF-α cytokine by ELISA method for testing the results showed that the treatment group in 15umol/L and 30umol/L concentration compared with the control group decreased significantly (P<0.05), while the expression of IL-10 increased significantly (P<0.05), with statistical significance.RT-qPCR and Western-blot method results show that the NF-κBp65 factor expression significantly reduced compared with control group (P<0.05), with statistical significance.ConclusionH2S play a protective role in atherosclerotic lesions by reducing the NF-κBp65 factor expression, and provide a valuable reference for the development and clinical treatment of H2S donor-type drugs.

Objective To investigate the relationship between serum levels of D-dimer(DD),hs-CRP and IL-6 with the infarction severity and prognosis in the patients with cerebral infarction(CI),and to analyze the influencing factors of prognosis for screening the independent risk factors affecting prognosis.Methods Eighty-one patients with CI in our hospital from January 2014 to June 2015 were selected as research subjects.All patients were treated with the CI routine treatment after admission.On admission,the CI severity was assessed by NIHSS,the serum levels of DD,hs-CRP and IL-6 were detected.After discharge,following up lasted for 12 months,the prognosis was assessed by MRS scale.The correlation between DD,hs-CRP and IL-6 with the infarction severity and prognosis was analyzed.The prognostic influencing factors were analyzed by adopting the univariate analysis and multivariate Logistic regression analysis.Results On admission,the levels of DD,hs-CRP and IL-6 in the severe group were significantly higher than those in the moderate group(q=5.736,7.772,4.757,P<0.05) and the mild group(q=11.094,16.920,12.255,P<0.05),while the moderate group was significantly higher than the mild group(q=5.783,9.870,8.049,P<0.05).Pearson′s analysis showed that the levels of DD,hs-CRP and IL-6 were positively correlated with the CI severity(r=0.603,0.457,0.394,P<0.05).After following up for 12 months,the levels of DD,hs-CRP and IL-6 in the good prognosis group were significantly lower than those in the poor prognosis group(t=4.775,3.101,2.910,P<0.05).Pearson′s analysis showed that DD,hs-CRP and IL-6 levels were positively correlated with poor prognosis(r=0.584,0.513,0.432,P<0.05).Multiple Logistic regression analysis showed that infarct volume,NIHSS score,DD,hs-CRP and IL-6 were the independent risk factors for poor prognosis in the patients with CI(P<0.05).Conclusion The infarct volume,NIHSS score,DD,hs-CRP and IL-6 are the independent risk factors for poor prognosis in the patients with CI,which provide a certain reference for judging the prognosis in CI patients.

Developing nationality medicine,to research the relationship between theory of Yi nationality medicine and correlated theories of traditional Chinese medicine. Panzhihua city is located in the juncture of Sichuan and Yunnan,and the Yi nationality inhabitants are more,so to understand and develop the Yi nationality medicine is very necessary.This question for discussion though the methods of investigating and studying on the spot and literatures collecting et al,found the characteristics and shortages of theory of Yi nationality medicine as well as the similarities and differences of theory of Yi nationality medicine and correlated theories of traditional Chinese medicine,and putted in discussion.Developing the Yi nationality medicine,in theory we need further excavation and unscrambling.

Delirium is a common disorder in aged people.Although it is in poor prognosis,delirium still has been under-recognized and inappropriately evaluated and treated.The clinical aspects of delirium on epidemiology,diagnosis,risk and precipitating factors,prevention and treatment were introduced in this paper.

ObjectiveTo evaluate the efficacy of nerve stimulator-guided thoracic paravertebral block in patients undergoing segmental mastectomy.MethodsFifty ASA Ⅰ or Ⅱ female patients,aged 18-64 yr,with body mass index ＜ 24 kg/m2,undergoing segmental mastectomy,were randomly divided into 2 groups ( n =25 each):thoracic epidural block group (group A) and nerve stimulator-guided thoracic paravertebral block group (group B).In group A,an epidural catheter was placed at T3,4 interspace and 0.5％ ropivacaine 7-10 ml was administrated via the epidural catheter.In group B,thoracic paravertebral block was performed at T4 under the guide of the nerve stimulator and 0.5％ ropivacaine 25 ml was administrated.The perioperative adverse cardiovascular events (hypotension and bradycardia) were recorded.Shivering,chest distress,and dyspnea during operation and nausea and vomiting after operation were also recorded.The range of block on the operated side was determined by pinprick test.The efficacy of anesthesia was evaluated.ResultsThe incidence of shivering,chest distress,and dyspnea during operation and nausea and vomiting after operation was significantly lower in group B than in group A (P ＜ 0.05).Adverse cardiovascular events and respiratory depression were not found in the two groups.There was no significant difference in the range of block on the operated side in the two groups ( P ＞ 0.05).The satisfactory anesthesia was found in 100％ of cases.ConclusionNerve stimulator-guided thoracic paravertebral block can be safely and effectively used in patients undergoing segmental mastectomy and the efficacy is better than that of thoracic epidural block.