Objective To discuss the treatment effect of doctor-nurse cooperation in multi-plane operation of obstructive sleep apnea hypopnea syndrome.Methods 73 patients with multi-planar surgery due to OSAHS were divided into the observation group (38 cases) and the control group(35 cases) in our department from January 2008 to December 2012.Health care was strengthened throughout the course of treatment in the observation group and traditional treatment and care were adopted in the control group.Short-term and long-term efficacy,intraoperative and postoperative complications were compared between two groups.Results The operation of short-term effective rate was 100％,and significant differences were found in the long-term efficacy between the two groups.There were significant differences in nasal bleeding during surgery,one-time intubation success rate and the significances were found in postoperative pain,extubation time,lung infection and soft palate regurgitation between two groups.Conclusions The implementation of the multi-plane surgery for patients with OSAHS can effectively improve the short-term efficacy of the patients throughout the course of treatment.Strengthening doctor-nurse cooperation effectively improve the patients' long-term efficacy,reducing the patients' intraoperative and postoperative complications.

Objective To investigate the change of peripheral blooe natural killer(NK)cell subsets of patients with recurrent spontaneous abortion( RSA),in oreer to proviee the relevant scientific evieence for unexplainee recurrent spontaneous abortion( URSA) eiagnosis ane treatment. Methods Sixty-three RSA patients were raneomly eivieee into CRSA(n = 33)ane URSA(n = 30). Meanwhile 30 cases normal pregnancy women were selectee as control. Flow cytometry was appliee to eetect NK cell levels in patients with RSA ane its subsets percentage,ane then comparee to the control group. Results NK cells subset in RSA,URAS ane normal pregnancy women were(13. 77 ± 1. 53)% ,(13. 97 ± 1. 60)% ,ane(13. 65 ± 1. 74)%. There was no significant eifference among three groups( F = 0. 287,P > 0. 05). The percent of CD56 + CD16 - ,CD56 +CD16 + ,CD56 - CD16 + NK cell subsets in RAS group were:(70. 00 ± 2. 49)% ,(13. 76 ± 2. 13)% ,(7. 04 ± 1. 44)% respectively,ane(56. 50 ± 3. 94)% ,(22. 88 ± 3. 22)% ,(11. 24 ± 2. 21)% in URAS group, meanwhile(70. 48 ± 3. 21)% ,(14. 16 ± 2. 14)% ,(6. 53 ± 1. 98)% in normal pregnancy group. There was no significant eifference between RSA ane normal pregnancy group( P > 0. 05). However,There was markeely eifference between URSA with RSA or normal group(F = 182. 587,18. 118,58. 879,P < 0. 05). Conclusion NK cell activity function plays a key role in the eevelopment of URSA. The eetection of the percentage of NK cell subsets of women euring pregnancy will help RSA monitoring ane NK cell subsets can preeict the percentage change in the pregnancy outcome .

Epithelial-mesenchymal transition is closely related with invasion and metastasis of malig-nant tumor and is focused on invasion and metastasis in malignant tumor in recent years .Many researches dermon-strate that epithelial-mesenchymal transition is involved in invasion and metastasis of several malignant tumors , such as breast cancer ,ovary cancer and non -small cell lung cancer .The relevant signal pathways and molecular proteins are also studied .Research progress of epithelial -mesenchymal transition in non -small cell lung cancer for invasion and metastasis is reviewed in this article .

Objective To evaluate the security and the reliability of ligating the cystic duct and the cystic ar- tery with common silk suture as the substitute for titanic or biological clamps in laparoscopic cholecystectomy (LC) us- ing the suture and knot instrument developed by the authors. Methods One hundred and two laparoscopic chole- cystectomies were performed by the authors using the instrument. Results All patients were discharged in the post- operative fourth or fifth day without any complication. Conclusion The instrument used for LC was safe and reliable.

36 mm?Hg. No permanent cardiac pacemaker was implanted. Transit trifascicular was seen in 3 cases, of whom, 2 disappeared with 1 hour after the procedure and, 1 trifascicular disappeared with 3 days. Permanent branch blocks were demonstrated in 36% patients. Conclusion PTSMA was feasible for HOCM with controllable complications. Precise position was the key point to prevent the alcohol leakage which will induce large area myocardial infarction and cardiac shock. Further study was needed to appreciate the more long-term follow-up.

5cm hepatic cancer, the effective rate (CR+PR) was 60 8% (62/102), no change (NC) rate 39 2% (40/102), and the 1-, 2-, 3-year survival rates 67 6% (69/102), 49 1% (50/102) and 39 2% (40/102), respectively. The effective rates (CR+PR) in patients with bone tumor and lung cancer were 100%(12/12) and 60%(3/5), respectively. There were no other severe complications found. Conclusions Ultrasound-guided microwave intervention is a safe, effective and feasible method for hepatic cancer, lung cancer and bone tumor.

Malignant transformation is closely related to gene mutations.The tumor suppressor gene p53 codes for a transcription factor,P53 protein,plays a critical regulation role in oncogene expression,DNA synthesis and repair systems,and cell apoptosis.It has been demonstrated that p53 mutation occurs in more than 50% of cancer cells,which is thought to play a crucial role in malignant transformation.p53-based gene therapeutical approaches in vitro and in vivo aiming at a direct correction of the specific molecular defect in p53-mutated malignant cells,showed notable efficacy in numerous cancer types.In this review,we discussed the current advances in this field,focusing on adenovirus-mediated wild-type p53 gene therapy.

Objective To investigate the feasibility and safety of laparoscopic surgery of giant hysteromyoma.Methods A total of 56 cases of giant hysteromyoma(at least 1 lesion ≥ 9 cm in diameter) underwent laparoscopic operations from January 2003 to August 2006 in this hospital.There were 30 cases of laparoscopic myomectomy(LM),10 cases of laparoscopic-assisted vaginal hysterectomy(LAVH),1 case of total laparoscopic hysterectomy(TLH),and 15 cases of laparoscopic supracervical hysterectomy(LSH).Results All of the operations were successfully performed under laparoscope.No conversion to open surgery was needed.The operation time was 40～100 min(mean,52 min),and the hemorrhage volume was 10～100 ml(mean,45 ml).Postoperatively,ureterovaginal fistula was observed in 1 case,which was cured by a re-operation of laparoscopic ureteroneocystostomy.Conclusions Laparoscopic operation for giant hysteromyoma is safe and feasible.

Objective To evaluate efficacy and safety of laparoscopic surgery for ectopic pregnancy with hemorrhagic shock.Methods One hundred and thirty women with hemorrhagic shock due to ectopic pregnancy underwent laparoscopic surgery from January 2003 to August 2006,including salpingectomy in 80 cases,partial ovariotomy in 8 cases,uterus wedge resection in 15 cases,salpingotomy in 19 cases,and tubal lesion clearance in 8 cases.Results Laparoscopic surgery was successfully performed in all the cases.Intra-abdominal blood loss was 1000 to 3500 ml,with an average of 1500 ml.The operation time was 30-60 minutes(mean:40 minutes).No postoperative complication occurred.Follow-up was conducted in 106 cases,once in a week in the first month after discharge.Serial ?-hCG levels decreased gradually and reached the normal level 1-2 weeks after operation.Persistent ectopic pregnancy was not found in the studied cases.Conclusions Laparoscopic surgery is a safe and effective treatment for hemorrhagic shock due to ectopic pregnancy,given the skill of an experienced laparoscopist with the aid of optimal anesthesia and advanced cardiovascular monitoring.

Objective To investigate the mechanism of Chinese herbal medicine compound Tang Mai Ping (TMP) capsule to type 2 diabet in rats. Methods The models of type 2 diabetic rats were established by injecting lower dosage of streptozotocin (STZ, 35 mg/kg) to belly, and fed high sucrose and high fat diet. Modle rats were randomly divided into model group, TMP group, and Erjiashuanggu (EJS) group, at the same time normal control. The content of plasma insulin was detected by radio-immunicy, mRNA expressing of frame muscle of Glucose Transporter 4 (GluT4) gene was detected with hyvirdism in situ. Results After built model, levels of insulin were obviously ascended in rats, but the expression of GluT4 mRNA lower significantly (P