BACKGROUND: Several studies have demonstrated that degradable polycaprolactone film has no apparent influences on cellular immune function of rat critical organs including heart, liver, and kidney, and possesses the prerequisite of anti-adhesion formation.OBJECTIVE: To investigate the role of degradable polycaprolactone film in preventing rat peritoneal adhesion following surgery.DESIGN, TIME AND SETTING: The present randomized, controlled animal experiment was performed at the Department of Physiology, Jilin University.MATERIALS: A total of 120 male Wistar rats, weighing 180-220 g, were included in this study. Peritoneal adhesion was induced in all rats. Non-bio-degradable film was sourced from Wuhan Boster Company, China. Polycaprolactone film was purchased from U.C.C Company. England.METHODS: All 120 rats were randomly divided into 3 groups, with 40 rats per group: control, non-bio-degradable film, and polycaprolactone film. Peritoneal adhesion was induced in all rats. The non-bio-degradahle film and polycaprolactone film groups were subjected to peritoneal wound cover with non-bio-degradable film and polycaprolactone film, respectively. From day 1 following surgery, each rat daily received an intraperitoneal injection of 4 mL 2 g/L pseudomonas enzyme, for a total of 3 successive days.MAIN OUTCOME MEASURES: At 1, 3, 7, and 30 days following surgery, 10 rats were selected from each group for observation of peritoneal adhesion.RESULTS: Totally 120 rats were included in the result analysis. Peritoneal adhesion was successfully induced in rats from the control group. From day 7 following surgery, the peritoneal adhesion rate was significantly lower in the polycaprolactone film group than in the control group (P<0.01). In the non-bio-degradable film group, the peritoneal adhesion rate exhibited decreasing followed by increasing tendency; by day 30, the adhesion rate was significantly higher compared to the polycaprolactone film group (P<0.01).CONCLUSION: Polycaprolactone film is degraded by pseudomonas enzyme to avoid the new adhesion caused by foreign stimulation.

Objective To develop an advanced two-cuff technique orthotopic liver transplantation model in rats. Methods On the basis of the previous two-cuff-technique, some hypercoagulable blood was released from the recipient's PV and IVC before the cuffed donor PV and IVC were inserted into the lumen of the recipient's PV and IVC. The anastomosis of recipient's SVC was between the donor's diaphragm venaccaval hiatus and recipient's SVC. Results Every anastomosis was effective. The operative successful rate and one-week survival rate were 96.7?% and 90.0?% respectively. Conclusion This model could be used as an effective measure for the study of liver transplantation in rats.

Objective To investigate the effects of high molecular bio-degradable film on preventing peritoneal adhesion.Methods One hundred and twenty rats were divided into control group,non-bio-degradable film group and polycaprolactone(PCL) group.Animal models of peritoneal adhesion made by operations on rats serve as control group.In the study,non-bio-degradable film and bio-degradable,namely PCL films sensitive to pseudomonas enzyme,were put into the opening of peritoneum.The effect of the films on peritoneal adhesion was examined on 1,3,7 and 30 d after operation.Results Peritoneal adhesion was found in all the rats of control group and very low in PCL group and non-bio-degradable film group.Conclusion PCL film can effectively prevent the formation of postoperative peritoneal adhesion.

OBJECTIVE: To study the curative efficacy of Shuxuetong injection for vascular dementia(VD).METHODS: A total of 60 patients with VD were randomized to receive Shuxuetong injection(6 mL) added into normal saline(250 mL)q.d by drip infusion for 21 days(treatment group) or citicoline injection(250 mg) added into 250 mL normal saline q.d by drip infusion for 21 days(control group).In addition,both groups received basic treatment and nursing care,and the antihypertensive,hypoglycemic and hypolipidemic therapies were adopted based on the patients' severity of disease and complications.The efficacy was evaluated before and after treatment by using mini-mental state examination(MMSE),activities of daily living(ADL),transcranial Doppler(TCD) and P300 test.RESULTS: Compared to control group,the treatment group had significantly higher MMSE score and ADL score(P

Objective To determine the relationship of bone mineral density (BMD) with age and sex hormone in elderly men. Methods The BMD in orthotope lumbar vertebra, femur neck, greater trochanter, Wards triangle region and shaft of femur were measured by dual-energy X-ray absorptiometry (DEXA), and serum testosterone (TT) and estradiol (E2) were determined by chemiluminescence in 360 elderly men. According to age and BMD, the elderly men were divided into several groups. The differences in BMD, serum TT and E2 among the groups were compared. Results There were 48 cases in osteoporosis group, 72 cases in bone mass reduction group and 240 cases in normal control group. The BMD in femur neck, greater trochanter of femur, Wards triangle region and shaft of femoral were decreased with ageing (F=3. 038,3. 029,3. 024,3. 021, respective, all P<0.05). In>80 age group, the BMD in femur neck, greater trochanter of femur, Wards triangle region and shaft of femoral were (0. 70±0. 140), (0. 682±0. 185), (0. 629±0.211), (0. 986± 0. 160)g/cm2, respectively;In 70-80 age group, they were (0. 829±0. 156), (0. 765±0. 170), (0. 698±0.187), (1. 042±0. 190) g/cm2, respectively;In 60-70 age group, they were (0. 875± 0. 138), (0. 800±0. 130), (0. 731±0. 145), (1. 071±0. 125)g/cm2, respectively. The differences in BMD of L1-4 had no statistical significance among different age groups(F=2. 988, P>0.05). There was no difference in level of serum TT among normal control group, bone mass reduction group an dosteoporosis group (F=3. 032, P>0. 05), while the level of serum E2 in the three groups were (180. 6±62. 3), (130. 5±39.9) pmol/L and (110. 5±68. 5) pmol/L, respectively. Bone mass was decreased with the decreased serum E2 level(F=3. 059,P<0. 05). Conclusions BMD is declined with ageing, and the level of serum E2 may affect the occurrence of osteoporosis in elderly men.

Objective To study the association between vitamin D status and bone mineral density(BMD) in aged people.Methods A total of 118 patients admitted in cadre department aged (77.7±11.2) years were selected from July 2010 to May 2011.The BMD at lumbar spine (L1-4) and femoral neck was measured by dual energy X-ray absorptiometry (DEXA).According to BMD,patients were divided into two groups:osteoporosis and non-osteoporosis group.The data of serum 25-hydroxy vitamin D3 [25(OH)D3]concentration,height,weight and age of patients were collected at the same time.Results The age and body mass index (BMI) in osteoporosis group were (81.6 ±5.6) years and (22.5 ± 4.0 ) kg/m2,while (79.4 ± 6.9 ) years and (24.1± 4.2 ) kg/m2 in nonosteoporosis group (t=1.80 and -2.01,P＞0.05).The concentrations of serum 25(OH)D3 in two groups were(21.6± 10.3)nmol/L and (32.0± 13.8) nmol/L,respectively(t=-4.20,P＜0.01).And there were 95.3 ％ (41/43 )and 81.3 ％ (61/75) of patients whose serum 25 (OH)D3 level were ≤50nmol/L in osteoporosis and non- osteoporosis group,respectively(x2 =4.58,P＜0.05).Furthermore,the 25 (OH) D3 level was positively correlated with BMD at femoral neck(r=0.22,P＜0.05),but not correlated with BMD at L1-4 ( r=0.18,P＞0.05).Conclusions Vitamin D status is correlated with BMD at femoral neck in aged people.

For the high cost and mobility issues, a home uterine contraction pressure monitoring system based on Windows CE platform was developed. In this paper, the design of hardware circuit, micro-controller system and LabVIEW program based on Windows CE are discussed. The clinical validation experiment in hospital for this system was made and the experimental results show that this system complies with the trend that current medical equipment is becoming portable, homely and networked. Through real-time monitoring uterine contraction pressure, occurrence of premature birth and abortion can be prevented effectively.
Female ; Humans ; Monitoring, Physiologic ; instrumentation ; Pregnancy ; Uterine Contraction

Objective To investigate the role of mismatch repair gene hMLH1 in pancreatic carcinoma and its clinicopathological significance.Methods hMLH1 was extracted from 60 cases of pancreatic carcinoma tissues and 60 cases of normol pancreatic tissues.hMLH1 expression in pancreatic carcinoma and normal tissues was detected by SP immunohistochemical staining.Results The strong,weak and loss expression of hMLH1 in pancreatic carcinoma tissues and normal pancreatic tissues was 0 vs 83.33％ (50/60),31.7％ (19/60)vs 16.67％ (10/60),and 68.3％ (41/60) vs 0 respectively.The protein expression of hMLH1 was not related to patient's age,tumor location,or pathological types (P ＞ 0.05),but it was related to lymph node metastasis (x2 =8.579,P =0.004),clinical stage (x2 =9.586,P =0.002) and pathological differentiation (x2 =20.372,P =0.001).Conclusion The loss expression of hMLH1 has a correlation with pancreatic carcinogenesis,differentiation degree,and disease progression.

Objective To provide the utilization information of drugs in Chongqing ,which has been analyzed the statistical data from 18 Chongqing medical organizations'sales volume from 2005 to 2007. Methods We have investigated and graded the sum of the drug purchasing money,the significant medical varieties,and the pharmaceutical manufactories to analyze the latest situation during this three years. Results According to the analysis,the sales number was increasing at a rate of about 20% per year. Water,heat and balance of electrolyte were the most positive rate increased (106.85%). However,the vitamin sell was the negative one dropping down at the rate of 10.47%. In addition,there was only three pharmaceutical companies in Chongqing ranked top 100. Conclusion To predict the future of the drug purchasing money will still rise successively. Nevertheless,it is influenced by rectifying the domestic medicine market so that Chongqing pharmaceutical enterprises are developing slowly without new varieties of drugs being produced.

OBJECTIVE To evaluate the risk factors of broad-spectrum antibiotic usage more than for two weeks and primary pulmonary disease as a risk factor of nosocomial infections. METHODS A retrieval report which used retrospective case control study to analyze the two risk factors of fungi nosocomial infection was reviewed. Fixed effect model or stochastic effect model were used for statistical analysis. RESULTS Three retrospective case control studyies were investigated. The primary pulmonary disease exposure rate was significantly higher than control group. Broad-spectrum antibiotic usage exposure rate had not significant difference. CONCLUSIONS Broad-spectrum antibiotic more than two weeks isn′t a risk factor but primary pulmonary disease is the risk factor for fungi nosocomial infection.