1.An analysis of the university student community development from the perspective of ideological and political education
Chinese Journal of Medical Education Research 2005;0(05):-
As the student communities develop rapidly,their activities become the new carrier of the ideological and political education work in universities.In his article,the author analyses the current situation,opportunities and challenges exerted by these communities on ideological and political education in universities,and discusses how to enhance the development of student communities from the perspective of ideological and political education as well.
2.Some Thoughts on the Course Design of the Aesthetics Education in Universities
Chinese Journal of Medical Education Research 2003;0(04):-
As a critical component of quality education,aesthetics education exerts profound significance on the cultivation of students featuring all-dimensional development and assumes an irreplaceable status in higher education.However,there are certain inadequacies in the course design of this regard.The thesis will investigate how to have a rational course design of aesthetics education to implement the teaching quality of aesthetics.
3.Distribution and schistosomiasis transmission risks of Biomphalaria stra-minea in inland China
Shaoyu HUANG ; Qiming ZHANG ; Xiaoheng LI ; Zhuohui DENG
Chinese Journal of Schistosomiasis Control 2014;(3):235-237
Biomphalaria straminea,the intermediary host of Schistosoma mansoni,was found by survey in local areas of Shen-zhen City in 1981,which was the first finding of the snail in inland China. By 2013,the snail had spread in large range of Shen-zhen City and overspread to the surrounding regions,Dongguan City and Huizhou City. Due to the facts that Shenzhen City has many international communications and is a key area of labor export,with high population mobility,while the reports of S. manso-ni infection in the returnees of our country from Africa have been increasing recently,it must be paid a high attention to whether the epidemic or transmission of schistosomiasis mansoni may happen under the present circumstance. This paper initially discusses the finding,distribution,spreading and overspreading and transmission risk of B. straminea in inland China and puts forward some related control suggestions.
4.Study on distribution of cohere antigen of 12D5 and 21B7 monoclonal antibodies of Angiostrongylus cantonensis and its application of immnodiagnosis
Renli ZHANG ; Muxin CHEN ; Yijie GENG ; Dana HUANG ; Shuang WU ; Shitong GAO ; Xiaoheng LI ; Xingquan ZHU
Chinese Journal of Microbiology and Immunology 2011;31(2):173-177
Objective To detect infection of Angiostrongylus cantonensis and examine effection of treatment to prepare monoclonal antibodies(McAbs). Methods Six-week-old BALB/c mice were imrnunized by the intraperitoneal injection of e/s antigens of Angiostrongylus cantonensis. Fusion of splecn cells from immunized mice with prepared SP2/0-Ag14 myeloma cells was performed in RPMI 1640. Fused cells were suspended in RPMI 1640 containing 1% HAT and 20% fetal calf serum and dispensed into 96-well cell culture plates. The supernatants of clones were screened by ELISA with sera of patients of angiostrongyliasis.Distribution of cohere antigen of 12D5 and 21B7 monoclonal antibodies was analyzed with immunohistochemistry. Two McAbs ( 12D5 and 21B7) were applied to detect the circulating antigen (CAg) in the sera of rats infected with A. cantonensis and angiostrongyliasis patients respectively by double antibody sandwich ELISA.Results 12D5 McAb was identified as IgG1 and 21 B7 McAb was IgM. Western blot result showed two McAbs could used to identified 55 × 103 protein of adult worms of A. cantonensis. Cohere antigen of 12D5 and 21B7 monoclonal antibodies were distributed on intestine surface of A. cantonensis. The detection rates of CAg in the sera of infected rats 100% (48/48), the detection rates of CAg in the sera of angiostrongyliasis patients was 100% (32/32). No cross-reaction to sera of patients with other infection of parasites, such as clonochiasis, fasiolopsiasis, ancylostomiasis, trichinosis, anisakiasis as well as schsitosomiasis, and health srea did not reacted with 12D5 and 21B7 McAbs,and detaction rate of antibody of angiostrongyliasis patients only reached 75% (24/32) with antigen of A. cantonensis. Conclusion Cohere antigen of 12D5 and 21B7monoclonal antibodies were antigens of enteric epithelium. Sandwich ELISA with 12D5 and 21B7 McAbs showed high specificity act as detecting CAg of A. cantonensis in sera of infection animal and patients. It is apparent that Sandwich ELISA with 12D5 and 21 B7 is not only rapid and simple without requirement of special instrument, but also rather sensitive and specific for the detection of current infection with A. cantonensis.
5.Molecular identification of the species of Biomphalaria snails in Shenzhen reservoir based on 16S rDNA sequences
Shitong GAO ; Xiaoheng LI ; Yijie GENG ; Xu XIE ; Shujiang MEI ; Da'na HUANG ;
Chinese Journal of Endemiology 2016;35(9):636-639
Objective To identify the species of Biomphalaria snails collected in Shenzhen reservoir,based on the mitochondrial 16S rDNA sequences.Methods The 16S rDNA fragments were amplified by PCR from the genome DNA of Biomphalaria snails,and inserted in plasmid pMD-18T for sequencing.The sequence of 16S rDNA fragment and its phylogenetic relationships with those of other species of Biomphalaria snails were analyzed with BLAST and MEGA4 software.Results The amplified 16S rDNA fragment of the Biomphalaria snails was about 466 bp in length.As aligned with the corresponding sequences of the related Biomphalaria species,the identity of nucleotides was 99% with 1 isolate of Biomphaltria straminea (B.straminea),98% with 3 isolates of B.kuhniana,95% with 1 isolate of B.intermedia,and 94% with 1 isolate of B.edisoni.Based on the 16S rDNA sequence,the results of phylogenetic analysis with neighbor-joining (NJ) and unweighted pair-group method with arithmetic means (UPGMA) indicated that the snails had close genetic relationships with the B.straminea isolate (Genbank accession NO.AY030213.1) Conclusion The Biomphalaria snails collected in Shenzhen reservoir could be classified as B.straminea based on the characteristics of 16S rDNA sequence.
6.Coupling between adherent leukocytes and blood flow.
Xiaoheng LIU ; Hua HUANG ; Huaiqing CHEN
Journal of Biomedical Engineering 2005;22(5):956-994
A theoretical model was developed to simulate the coupling between the adhered leukocyte and blood flow. Methods of computational fluid dynamics (CFD) were introduced to analyze the distributions of the shear stress and the pressure on the cell surface. Meanwhile, the hemodynamical parameters were measured with laser Doppler velocimetry. The results showed that the deformation index of the leukocyte was increased with initial contact angle and Reynolds number. The blood flow induced the redistributions of the shear stress and pressure on the surface of the cell, but the maximum shear stress was on the top point of the cell. It suggests that the non-uniform distribution of shear stress on the cell surface may play a particular role in the change of cell shape and functions.
Blood Circulation
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physiology
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Cell Adhesion
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Hemodynamics
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Humans
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Leukocytes
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cytology
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physiology
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Models, Biological
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Regional Blood Flow
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Shear Strength
7.Surveillance of antimicrobial resistance in clinical isolates from intensive care units during 2012
Li YAN ; Siqiang NIU ; He CAO ; Ping YANG ; Xiaobing ZHANG ; Shifeng HUANG ; Xiaoheng ZHANG ; Shan SUN ; Xiuli HE
Chongqing Medicine 2013;(25):3022-3023,3025
Objective To investigate the antimicrobial resistance of main clinical isolates from ICU during 2012 .Methods Auto-matic microbiology analysis system and the disk diffusion method were performed to determine the antimicrobial susceptibility .All the data were analyzed by WHONET5 .6 software according to the breakpoints of The American Association of Clinical Laboratory Standardization Institute (CLSI) 2012 .Results A total of non-repeated 1 374 clinical isolates were collected in ICU during 2012 , including 1 089 strains (79 .3% ) of Gram-negative bacilli and 285 strains (20 .7% )of Gram-positive cocci ;The top five pathogens were Acinetobacter baumannii ,Pseudomonas aeruginosa ,Escherichia coli ,Klebsiella pneumoniae and Staphylococcus aureus ;Some Enterobacteriaceae strains were resistant to imipenem or ertapenem .2 strains of Enterococcus faecium were found resistant to van-comycin .Conclusion Non-fermenting bacteria ,Enterobacteriaceae and Staphylococci remain the predominant pathogens isolated from the patients in ICU ,their drug resistance is serious ,it is important to use antibacterial agents rationally and strengthen the sur-veillance of bacterial drug resistance .
8.Numerical simulation of the distribution of shear stress on the bottom of parallel plate flow chamber under different inlet velocity conditions.
Ye ZENG ; Xiaoheng LIU ; Yi LAI ; Xianliang HUANG ; Bin MAO ; Ting GAO ; Yang SHEN
Journal of Biomedical Engineering 2010;27(4):785-789
The distribution of shear stress on the bottom of the parallel plate flow chamber under different inlet velocities was analyzed by numerical simulation. In the present experimental study, the projection planes of the relative errors at 0.7% level were obtained, and then the efficient region and the actual entrance length were further corrected by introducing the concept of relative error. The results showed that the efficient region of the chamber increased with the direction of length while the inlet velocity was increased, and the actual entrance length was much greater than that of the theoretical entrance length. Therefore, in accordance to the needed range of shear stress in experiment and to the needed efficient region area, the optimum design of the flow chamber is necessary.
Algorithms
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Blood Flow Velocity
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Blood Pressure
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physiology
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Computer Simulation
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Humans
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Models, Cardiovascular
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Numerical Analysis, Computer-Assisted
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Pulsatile Flow
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Rheology
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Shear Strength
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Stress, Mechanical
9.Parent-child cohesion and problematic mobile phone use of middle school students in Shenzhen
CHEN Dingyan, ZHOU Li, HUANG Yuanyuan, LI Xiaoheng
Chinese Journal of School Health 2019;40(9):1342-1344
Objective:
To understand parent-child cohesion and problematic mobile phone use among middle school students in Shenzhen, and to provide a refevence for conducting the relevant control and prevention work .
Methods:
A total of 3 992 students from 5 junior high schools and 5 senior high schools in Shenzhen were selected by using stratified cluster sampling method. Problematic mobile phone use and parent-child cohesion were investigated, and their association was analyzed.
Results:
The average score of father-child cohesion and mother-child cohesion was (34.34±9.67) and (38.14±8.35), respectively. Mother-child cohesion score was higher than father-child cohesion score in both junior and senior middle school students(t=12.07, 14.41, P<0.01). Father-child cohesion (r=-0.20, P<0.01) and mother-child cohesion (r=-0.17, P<0.01) was negatively correlated with problematic mobile phone use. The lower the score of parent-child cohesion, the higher the score of problematic mobile phone use.
Conclusion
Family plays an important role in the prevention and control of problematic mobile phone use among middle school students. Improving parent-child cohesion and building a good parent-child relationship may help prevent problematic mobile phone use.
10.A system for testing alternating frequency properties of body fluid and tissues.
Hua HUANG ; Zhirun YUAN ; Huaiqing CHEN ; Jiangang XIA ; Xiaoheng LIU ; Zhong WANG ; Qi LIU ; Yi HE
Journal of Biomedical Engineering 2004;21(6):939-942
The electric properties of biotic tissues are of importance to our understanding biotic substances even life. But the measurement taken in this aspect is different from the measurement of ordinary materials, we need a special testing instrument for living substances. In order to understand the alternating frequency properties of body fluid and tissues in detail, we have developed an electricity characteristic testing system, which is more proper for testing living substances. This system adopts advanced measuring method and expert circuit. As the measure and the control core, the single chip processor is used. This system has wide frequency range and wide impedance testing range, much more adapted objects, and it is dependable, more precise and intelligent. The application of this system in practical testing has obtained optimal results.
Body Composition
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physiology
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Body Fluids
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physiology
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Electric Impedance
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Electrophysiology
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Humans
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Models, Biological
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Systems Theory