ObjectiveTo explore the surgical techniques, efficacy and the management of complicated ocular trauma with anterior-posterior segment complications such as cyclodialysis. Methods Fifty-five patients (55 eyes) with complicated ocular trauma were enrolled in this study. Among them, there were 35 cases with eyeball contusion and 20 cases of eyeball rupture. Preoperative visual acuity was from no light perception to 0. 15, intraocular pressure (IOP) ranged from one to 10 mm Hg (1 mm Hg=0. 133 kPa).Cyclodialysis, vitreous hemorrhage or retinal detachment were revealed by B-ultrasound and ultrasound biomicroscopy (UBM). Cyclodialysis clefts ranged from one to 12 clock-hours. All patients underwent 3-port pars plana vitrectomy with gas/silicone oil tamponade and ciliary body reattachment by cryotherapy (cyclodialysis clefts ≤ three clock-hours) or suture fixation ( cyclodialysis clefts ＞ three clock-hours).Healing after surgical trauma, visual acuity, intraocular pressure, intraocular hemorrhage, ciliary body and retinal reattachment were followed up. Results In one month after surgery, UBM showed 54 patients out of 55 patients had good ciliary body reattachment. Gonioscopy revealed cyclodialysis still existed in one patient, and this was cured by a second surgical suture fixation. In three months after surgery, ocular trauma healed in all 55 patients, the visual acuity ranged from no light perception to 0. 15, with a best corrected visual acuity of 0.8. The ciliary body and retina had good reattachment. IOP of 52 patients was normal; IOP of three patients was still lower than 10 mm Hg. Three patients had secondary glaucoma which was treated by glaucoma surgery. Conclusions Complicated ocular trauma with cyclodialysis can be treated with vitrectomy and cryotherapy or transscleral sutures. The procedure is safe and effective.

Objective:For further exploring the feasibility,we have treated the middle and infe- rior femoral bone fracture nonunion by the transplantation of bone flap with ascending and transvers branch of the lateral femoral circumflex vessels.Method:The technique,on the basis of anatomical study have been designed to treat the femoral bone fracture nonunion by the transplantation of bone flap with ascending and transvers branch which pedicle was descendant branch of the lateral femoral circumflex vessel.Results:8 cases of middle and inferior femoral fracture nonunion had been treated by this method.At follow-up of 1 year 5 months to 4 years 6 months,the results have been satisfacto- ry.Conclusions:The method of the transplantation of the bone flap with ascending and transvers branch of the lateral femoral circumflex vessel for the treatment of middle and inferior femoral fracture nonunion has the adventages of simplicity,easiness,reliability.

Retinopathy of prematurity (ROP) is a potentially blinding retinal vascular disease that occurs in premature infants and low birth weight infants.Along with the development of perinatal medicine,the incidence of ROP has increased.If infants with ROP could get treatment in time,the disability rate due to ROP would significantly reduce.Now,the present situation and advances in treatment of ROP were reviewed.

Total flavones of hippophase rhamnoides (TFH) inhibited the contraction of isolated rabbit aortic preparations induced by KCl, CaCl_2 and noradrenaline (NE), shifted the dose-response curvesto the right and depressed the maximal response. PD_2' values were 5.79, 5.75 and 5.81 respectively. TFH could also reduce Ca~(2+) -dependent contraction in rabbit aortic preparations. These results suggest that relaxation of vascular smooth muscles caused by TFH may be due to TFH natagonistic effect on transmembrane Ca~(2+) channels.

Objective To evaluate the surgical technique in ventricular septal defect(VSD)associated with aortic valve insufficiency(AI)patients.Methods 115 VSD patients associated with AI were retrospectively analyzed.96 patients belong subarterial to VSD,membranous defect in 19 cases.Moderate hypothermia circulatory arrest in all cases.Direct closure of the VSD was undertaken in 16 cases and other cases repaired with patches.Aortic valvuloplasty in 92 cases.and 5 patients underwent aortic valve replacement.The other 18 cases were directly repaired the VSD with patches.Results The results of surgical treatment is satisfactory.In the 4 months to 2 years follow up,10 patients died,NYHA cardic function 72 cases is Ⅰ,31 is Ⅱ and 2 is Ⅲ.Conclusion Early and concomitant surgical treatment ventricular septal defect associated with aortic valve insufficiency can obtain an ideal results.

Objective To construct the recombinant plasmid pEGFP-N1-SrV+ and evaluate the expression of SrV+in mammalian 293T cells.nethods srv+.a gene encoding the vailable region of the surface protein of the Streptococcus mutans OMZ175.was cloned chemically based on its reported nucleotide sequence.The eukaryotic expression plasmid,pEGFP-N1-SrV+,was constructed by introducing the srv+ gene into the Kpn Ⅰ/Xho Ⅰ site of pEGFP-NI.The recombinant plasmid pEGFP-N1-SrV+was transfected into 293T cells with lipofectamine and the expression level of SrV+was evaluated.Results The eukaryotic expression plasmid pEGFP-N1-SrV+was constructed successfully.GFP was observed by green fluorescent microscope.and a 72 × 1 03 protein was detected bv Westem blot.Real-time RT-PCR analysis revealed that the expression of the pEGFP-N1-SrV+in 293T was excellent and significant compared the control group. Conclusion The recombinant plasmid pEGFP-N1-SrV+was successfully constructed.which could encode the expression of SrV+after transfected into the mammalian 293T Cells.

Objective To study the average difference of CHN atlas method bone age (BA) determination (left-vs.right-hand) for children and adolescents.Methods Radiographs of hands of healthy children and adolescent (age range:1.0-19.0 years) were analysed to determine BA,producing analysis results for 1380 hand images.The images were assessed by two radiologists based on CHN atlas method,who were blinded to the chronological age (CA) of them.The intra-and inter-observer variations were used for evaluating the reliability of CHN atlas method.The difference of BA using left-or right-hand BA was evaluated by t test.Results No significant difference was found for intra-or inter-observer variations (P ＞ 0.05).The BAs of left-and right-hand radiograph showed significant difference in the groups for boys (16.0-16.9,17.0-17.9 years),girls (6.0-6.9 years) and both of them (6.0-6.9,16.0-16.9 years).The regression model analysis showed no significant difference(t =-0.427,P =0.670).Conclusion Comparison of left-and right-hand BA shos no significant difference using CHN atlas method,indicating that either hand could be used to determine BA.

Objective:To investigate the regulation of proliferation and differentiation of bone marrow mesenchymal stem cells(BM-SCs)by CKIP-1 in vitro.Methods:BMSCs from CKIP-1 nock out(KO)and wild type(WT)C57 mice were isolated and cultured u-sing adherence method in vitro.BMSCs of the 3rd passage were induced to osteogenic and adipgenic differentiation.Cell proliferation was examined by MTT assay.Cell surface markers were tested by FCM.The osteogenic and adipogenic differentiation was studied by alkaline phosphatase (ALP)staining,alizarin red staining and oil red O staining.Results:The proliferation and cell marke expression of the 2 groups were similar.ALP staining of KO group was strong than that of WT group after osteogenic induction.Alizarin red stai-ning showed that there were more mineralized nodules in BMSCs of KO group than in those of WT group.Oil red O staining of KO mice BMSCs was stronger than that of WT.Conclusion:CKIP-1 deficiency can enhance the osteogenic and adipogenic differentiation without influence on the proliferation of BMSCs.

Objective:To investigate the feasibility of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI)parameters in differentiating musculoskeletal tumors with different behaviours of pathological findings before therapy.Methods:A total of 34 subjects of musculoskeletal tumors were in-volved in this retrospective analysis.DCE-MRI was performed using a fat-saturated 3 D VIBE (volumetric interpolated breath-hold exam)imaging sequence with following parameters:FA,10 degree;TR/TE, 5.6/2.4 ms;slice thickness,4.0 mm with no intersection gap;field of view,310 mm ×213 mm;ma-trix,256 ×178;voxel size,1 .2 mm ×1 .2 mm ×4.0 mm;parallel imaging acceleration factor.The ac-tuation time for the DCE-MRI sequence was 255 s with a temporal resolution of 5 s and 40 image vo-lumes.Using pathological results as a gold standard,tumors were divided into benign,borderline and malignant tumors.Toft’s model was used for calculation of Ktrans (volume transfer constant),Ve (extra-vascular extracellular space distribute volume per unit tissue volume)and Kep(microvascular permeability reflux constant).Those parameters were compared between the lesions and the control tissues using paired t-tests.The one-way analysis of variance was used to assess the difference among benign,border-line and malignant tumors.P values <0.05 difference was statistically significant.Results:Based on the WHO Classification of Tumours of Soft Tissue and Bone(2012)criteria,34 patients were divided into three groups:1 1 for benign tumors,12 for borderline tumors,and 1 1 for malignancies.Compared with control tissues,Ktrans and Kepshowed no difference,but Ve was increased in benign tumors,Kep showed no diffe-rence,butKtransandVewereincreasedinborderlinetumors, Ktrans,KepandVewereincreasedin malignant tumors.Ktrans(P<0.001 )and Kep (P<0.01 )were significantly higher in malignant tumors than in benign and borderline tumors,but did not show any difference between benign tumors and border-line tumors.Ve was significantly higher in malignant tumors than in benign (P<0.05),but did not show any difference between malignant and borderline tumors,benign tumors and borderline tumors (P >0.05 ).Conclusion:DCE-MRI technique is useful to evaluate the pathological behaviour of musculoske-letal tumors.The quantitative analysis of DCE parameters in conjunction with conventional MR images can improve the accuracy of musculoskeletal tumor qualitative analysis.

BACKGROUND:Studies have shown that there are large differences in the thickness of the soft tissue overlying hard tissue, and the soft tissue does not uniformly overly the hard tissue, indicating simple hard tissue measurement wil not harvest ideal facial profile in clinical treatment of malocclusions. OBJECTIVE: To study the craniofacial soft and hard tissue characteristics in the adult Angle class II malocclusion, and then to analyze the relationship between Angle class II1 and class II2 malocclusions. METHODS: Sixty patients with adult Angle II malocclusion who were accepted by the Department of Orthodontics of Stomatological Hospital Affiliated to Jiamusi University from 2011 to 2014, on gender parity, aged 18-38 years (mean age of 26.3 years), including 30 cases of Angle class II1 and 30 cases of Angle class II2. Differences between the adult Angle class II1 and class II2 malocclusion patients were compared by cephalometric analysis based on X-ray measurement. Statistical correlation analysis was performed.RESULTS AND CONCLUSION:(1) Comparisons of hard tissue measurement of adult Angle class II1 and Angle class II2 malocclusions showed that: SNB, SND, ANB, FH-NP, U1-SN (P < 0.001), LI-NB (P< 0.01), L1-MP (P < 0.01), U1-L1 (P < 0.001) exhibited statisticaly significant differences between two groups (P < 0.05). (2) Comparisons of soft tissue measurement of adult Angle class II1 and Angle class II2 malocclusions showed that: there were significant differences in the ULA'-FH, LLNs-FH, ULNs-FH, CmSnUL, E-LL (P < 0.05). (3) There was a correlation between the soft and hard tissue of adult Angle class II1 and Angle class II2 malocclusions in al measurement indexes, but the correlativity exists differently. These findings indicate that for Angle class II1malocclusion, the maxilary and anterior teeth protrusions have a certain influence on the position of the lower lip; for Angle class II2 malocclusion, only maxilary protrusion can impact the position of the soft tissue of the lower lip. Chin soft tissue has no major changes in Angle class II2 malocclusion, but it varies greatly in Angle class II1 malocclusion. Clinical treatment of adult Angle class II malocclusions is developed based on the craniofacial soft and hard tissue characteristics in orthodontic and orthognathic surgeries.