Using the glass microelectrode technique and the inverted microscope, we have successfully recorded the transmembrane potentials in the clusters of cultured cardiac cells from theventricle of newborn rats. The amplitude of action potentials could reach 83.8?15.1 mV (?SD). This method is charaterized by less influence factors of internal environment, high susceptibility, etc. It might be widely applied to the cardiovascular research in physiology, pathophsiology and pharmacology,

Objective To discuss the teaching of neurobiology to improve the teaching quality. Methods 35 students in Grade 2010, 39 in Grade 2011 and 41 in Grade 2012 in our university were enrolled. Grade 2010 received traditional teaching only, Grade 2011 received problem-based learning (PBL) in addition, and Grade 2012 received traditional teaching, PBL and case discussion. The scores of final exami-nation were compared. Results The scores of final examination were higher in students in Grade 2012 than in Grades 2010 and 2011. Con-clusion The combination of traditional teaching, PBL and case discussion may further improve the teaching quality of students.

Objective To examine the association of characteristics of patients visiting fever clinic with different air quality index in Beijing.Methods Six days with highest ambient air quality index (AQI) (polluted weather group) and 6 days with lowest AQI (clear weather group) during February 1 st to March 31th 2014 were selected as study period.The clinical data of patients,who visited in fever clinic of Peking University Third Hospital during the selected 12 days,were retrospectively analyzed.Results There were 417 patients in clean weather group and 481 in polluted weather group.There were no significant differences in duration of fever [(2.30 ± 2.51) d vs.(2.18 ± 2.58) d,x2 =0.701,P =0.484] and highest body temperature [(38.60 ± 0.65) ℃ vs.(38.57 ± 0.66) ℃,x2 =0.545,P =0.586] between two groups.The symptoms of cough,sore throat in pollution group were higher than those in clear group (52.39％ vs.45.56％,x2 =4.166,P =0.041;50.52％ vs.42.21％,x2 =6.203,P =0.013,respectively).The proportion of cases diagnosed as influenza-like illness in polluted weather was higher than that in clear weather group (53.85％ vs.46.76％,x2 =4.484,P =0.034).Conclusion The results indicate that the symptoms cough and sore throat and influenza-like illness are increased in polluted weather in Beiiing.

Objective To investigate the effects of carbon monoxide poisoning on the long-term cognition in elderly patients.Methods Totally 53 patients with carbon monoxide poisoning and 51 healthy persons at our Hospital from June 2005 to June 2008 were enrolled.Mini-mental state examination(MMSE) and activities of daily living(ADL) scale were utilized to evaluale the life quality of the elderly patients through a cross-sectional study on July 2011.Results MMSE score showed that the attention calculation score,delayed recall score,and total score were higher in control group (4.9±0.4,2.9±1.0,28.3±2.3,respectively) than in carbon monoxide poisoning group (4.5±0.8,2.6±0.6,25.5±3.5,respectively) (t＝3.138,3.607,4.802,all P＜0.05).ADL score of carbon monoxide poisoning group was 26.7±7.3,and that of the control group was 2.04±7.6 (t＝1.827,P＞0.05).Conclusions Elderly patients with acute carbon monoxide poisoning may cause long-term cognitive impairment with decreasing attention calculation and delayed recall.

The antiarrhythmic actions of ZnSO4 (Zinc) were studied in ex-perimental arrhythmias models. ZnSO4 10mg/kg(iv) significantly coun-teracted the arrhythmias, induced by aconitine or BaCl2 rats.It was shown to shorten the duration of arrhythmias induced by chloroform-epinephrine in rabbits. In mice, ZnSO4 20 ?g/10g(ip) decreased the incidence of ventricular fibrillationbychloroform. However, ZnSO4 did not decrease the incidence of CaCl2-induced , venticular fibrillation, the threshold and lethal doseofouabain in producing ventricular permature beats, ventricular fibrillation in guinea pigs.

Zinc sulfate 0.1mmol/L inhibited the frequency dependent positive staircase phenomenon on isolated guinea pig atria. Ten min after adding zinc sulfate 0.1 mmol/L, the contractility of the left atrium decreased. The needed concentration of epinephrine inducing automaticity was increased and the functional refractory period(FRP) was prolonged from 192?5 ms to 247?4 ms ( P

As an important modality in the treatment of central nervous system malignancies of pediatric patients, radiotherapy could improve both local control and long-term survival.However, both short-term and long-term adverse effects are of concerns.In the current review, the role of radiotherapy in the pediatric setting were introduced.

Objective To analysis the clinical efifcacy of blue-ray combined with Yinzhihuang Keli for newborn pathological jaundice. Method 120 newborns with neonatal jaundice were randomly divided into control group(traditional treatment group, took enzyme inducers phenobarbital and continuous blue-ray radiation for 8 h everyday)60 cases and treatment group(Yinzhihuang Keli combined with traditional treatment group)60 cases,7 days for a course of treatment , then the efifcacy of two groups were compared. Results After 7 days treatment, we found compared with control group, the speed of decline of serum bilirubin of treatment group was much faster, the degree of decline was much bigger and the average time of jaundice subside was much shorter, all P<0.05.The total efifciency of treatment group was 98.33%which was signiifcantly higher than the control group which was 91.67%, P<0.05.Conclusion Compared with conventional treatment methods, blue-ray combined with Yinzhihuang Keli for neonatal jaundice would get a better efifcacy, faster speed of jaundice subside and smaller side effects.