Objective To investigate the expressions and significance of IL-22 and related cytokines (IL-23pl9 and IL-6) in sera and PBMCs of patients with psoriasis. Methods Sera and PBMCs were obtained from the venous blood samples from 58 patients with psoriasis vulgaris and 20 normal human controls. The PBMCs were subjected to culture for 5 hours followed by the collection of cells and culture supernatant. Then,quantitative real-time RT-PCR was used to examine the mRNA expressions of IL-22, IL-23pl9 and IL-6 in PBMCs, enzyme-linked immunosorbent assay (ELJSA) to detect the level of IL-22 protein in the sera and culture supernatant of PBMCs. Results In the patients with psoriasis and controls, the relative expression level in PBMCs was 4.48 ± 2.64 and 2.35 ± 0.91 respectively for IL-22 mRNA, 6.07 ± 4.09 and 2.61 ± 1.46 respectively for IL-23pl9 mRNA, 3.87 ± 1.49 and 1.48 ± 0.62 respectively for IL-6 mRNA; significant differences were observed between the two groups in all the above parameters (all P < 0.01). ELISA revealed that the level of IL-22 protein in the patients and controls was (86.23 ± 25.58) ng/L and (43.67 ± 14.82) ng/L respectively in the sera (P< 0.01), (119.11 ± 21.51) ng/L and (57.70 ± 13.17) ng/L respectively in the culture supernatant of PBMCs (P< 0.01). Conclusion There is an overexpression of IL-22 in the PBMCs and sera of patients with psoriasis, implying that IL-22 is involved in the pathogenesis of psoriasis.

Objective To explore the lipid metabolism in patients with hypertension and obstructive sleep apnea-hypopnea syndrome.Methods A total of 896 patients (655 cases of male; 241 cases of female) was recruited who were hospitalized in our department,and were classified into four groups based on the finding of polysomnography (PSG):hypertensive without obstructive sleep apnea-hypopnea syndrome (OSAS) (n =243),hypertensive with mild OSAS (n =245),hypertensive with moderate OSAS (n =195),and hypertensive with severe OSAS (n =213).Multiple indices including apnea-hypopnea index (AHI),lowest arterial oxygen saturation(lowest SaO2),body mass index (BMI),blood pressure,total cholesterol (TC),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDLC),triglycerides (TG),fasting blood glucose(FBG),uric acid (UA),and high-sensitivity C-reactive protein(hs-CRP) were assessed,and the relevant risk factors of lipid metabolism were analyzed.Results (1)Male patients had more opportunities to suffer OSAS than female (P ＜0.01).Compared with the group without hypertensive,patients in severe OSAS group had higher levels of AGE (48.09 ± 9.48,BMI (29.46 ±3.83),AHI[45.90(37.55,63.65)],MSpO2 (89.08 ±4.93),LSpO2 (67.36 ± 12.60),TC (4.68 ±1.00),TG[2.03(1.54,2.88)],UA (371.85 ±99.29),and hs-CRP[1.43(0.82,3.056)] (P ＜0.05),and had lower levels of HDL-C (1.09 ± 0.28).(2) Two and more than two lipids abnormal metabolic indices increased prevalence with the increase of the severity of OSAS.(3)The prevalence of high TG,high TC in AHI ≥ 15/h was significantly higher than AHI ＜ 15 group.(4) After adjustment for BMI,gender,age and other common risk factors,it confirmed that AHI was still related to lipid metabolism.AHI was an independent risk factor for abnormal lipid metabolism.Conclusions AHI was an independent risk factor for abnormal lipid metabolism.With increasing severity of OSAS,the levels TC,TG,and the category of abnormal lipid metabolism were also increased.

Objective To explore the relationship between genetic polymorphisms of sixtransmembrane protein of prostate 2 (STAMP2) and metabolic index,TNFα in Xinjiang Uygur population.Methods STAMP2 gene functional regions were sequenced in Uygur Xinjiang population diagnosed as metabolic syndrome.Patients were divided into the following three groups by their TNFα concentration:the high level group(TNFα≥7.95 μg/L,n =313),the moderate level group(TNFα ＞5.34-＜7.95 μg/L,n =268)and the low level group(≤5.34 μg/L,n =313).The selected representative variations were genotyped by TaqMan-PCR in 894 Uygur individuals.The association of the genetic variations of STAMP2 gene with metabolic index and TNFα was analyzed.Results Three representative variations were genotyped,including rs8122,rs1981529 and rs34741656.The genotype distribution and allele frequencies of rs8122and rs1981529 were statistically different among the three groups (P ＜ 0.05),while no difference was observed with rs34741656(P ＞0.05).By ANOVA analysis,statistical difference was showed between the rs1981529 polymorphism AA and AG in the concentration of TNFα(P ＜ 0.05).None of the polymorphisms was significantly associated with TC,HDL-C,LDL-C and TG (P ＞ 0.05).Conclusion Two STAMP2 gene polymorphisms,rs8122 and rs1981529 are associated with the concentration of TNFα in Xinjiang Uygur population.

Parathyroid hormone/parathyroid hormone-related peptides are polypeptide hormones. They have similar gene struc-ture and same membrane receptor .They play great roles in regulating the calcium and phosphorus metabolism in vivo.PTH/PTHrP and their receptors are expressed in tissues of tumor ,skin, hair follicle and other normal tissues .Because of their physiological action on the proliferation and differentiation of epidermis and hair growth ,they may be a potential therapeutic target for some skin diseases including psoriasis.

Objective To investigate the changes and related factors of maternal thyroid autoantibodies during early pregnancy. Methods Urinary iodine concentration( UIC) , serum thyroid stimulating hormone( TSH) , free thyroxine ( FT4 ) , thyroid-peroxidase antibody ( TPOAb ) , thyroglobulin antibody ( TgAb ) concentrations were determined in 7 190 women during early pregnancy in an iodine-sufficient region of China. Results The prevalence of TPOAb positivity and TgAb positivity were 8. 7% and 12. 0% respectively. The prevalence of overt hypothyroidism and subclinical hypothyroidism increased significantly in group of thyroid antibody positivity. The prevalence of TPOAb positivity and TgAb positivity presented a U-shaped curve, ranging from mild iodine deficiency to iodine excess, especially increased significantly in the group with UIC<100 μg/L. Conclusion Prevalence of thyroid antibodies positivity became higher during early pregnancy. The positive thyroid autoantibodies during pregnancy were significantly associated with maternal hypothyroidism. Both iodine excess and iodine deficiency are risk factors of positive thyroid antibodies.

Objective To investigate the role of complement in radiation-induced lung injury in mice after chest irradiation with ⁶⁰Co γ-rays at a single dose of 20 Gy. Methods C57BL/6 mice underwent chest irradiation with ⁶⁰Co γ-rays at a single dose of 20 Gy, followed by observation for the inflammatory reaction of the lung tissue in the early stage (within 15 d) and pulmonary fibrosis in the later stage (30 and 180 d). Enzyme-linked immunosorbent assay was used to measure the levels of C2, C3a, C4, and C5b-9 in the lung tissues at 1, 3, 7, 15, 30, and 180 d after irradiation. The expression of complement mRNA in BEAS-2B cells after irradiation was determined using RT-PCR. Results Radiation-induced lung injury in micepresented as inflammatory response in the early stage and fibrosis in the late stage. Complement C2, C4, and C5b-9 complexes were increased in the early period (3 or 7 d) after irradiation (P < 0.05), which might be associated with the inflammatory response induced by irradiation. During 3 to 180 d, complement C3a was significantly higher in the irradiated mice than in the control mice, suggesting a close relationship between C3a and radiation-induced lung injury. The irradiated cells showed increased mRNA expression of C2 and C3, with no changes in the mRNA levels of C4 and C5. Conclusion Different complement proteins have varying responses to radiation-induced lung injury, among which C3a is closely related to radiation-induced lung injury, suggesting that regulating C3a and its receptors may be a new way to prevent and treat radiation-induced lung injury.