Objective:To observe the clinical efficacy of nitrous oxide inhalation in oral and maxillofacial surgery.Methods:1 80 pa-tients underwent maxillofacial surgery were randomly divided into 2 groups(n =90).The patients in group A were given nitrous oxide inhalation before surgery,those in group B were served as the controls.The blood pressure(BP),heart rate(HR)and oxygen saturation of blood(SpO2 )were monitored during surgery,the feelings of the patients for surgical procedures,pain VAS score and Houpt behavior rating scale were surveyed after surgery.Results:In the whole operation process,BP,HR and SpO2 of group A changed smoothly(P >0.05),BP and HR of group B increased during operation(P <0.05),SpO2 had no obvious change(P >0.05);Frankl treatment adher-ence score in group A and B was 3.66 ±0.05 and 2.91 ±0.05 respectively(P <0.05);Houpt behavior rating scale were 5.06 ±0.1 1 and 3.29 ±0.06 respectively(P <0.05).VAS score in group A was lower than that in group B(P <0.05).Mild adverse reaction was observed in 6 cases in group A.Conclusion:In the process of maxillofacial surgery,nitrous oxide inhalation has sedation,analgesia and anti-anxiety effects without severe adverse effect.

Objective To study the expression of interleukin-13(IL-13) in renal tissues of several common pathologic types of primary glomerulonephritis(PGN).Methods Immunohistochemistry was used to examine the expression of IL-13 in renal tissues of 81 patients with PGN and 10 normal control cases.The correlation analysis was performed with biochemical and pathological indicators.Results Extensive expression of IL-13 was found in renal tissues of patients with PGN.The expression of IL-13 in renal tissues was positively correlated with the concentration of blood urea nitrogen,serum creatinine,the quantitation of urine protein and the crescent formation in glomerular(r=0.325,0.275,0.291,0.231,respectively).It was negatively correlated with glomerular filtration rate and serum albumin(r=-0.448,-0.296,respectively).Conclusion IL-13 participates in the process of inflammation reaction and crescent formation in the renal tissues in patients with PGN,and is correlated with the progression of renal function and the level of urea protein.

Objective To research the mechanism of electroacupuncture(EA) improving morphine abstinence syndrome in rats.Methods The model of the rat with physical morphine abstinence syndrome was established.The expression of ?-opioid receptor mRNA in the brain tissue of morphine withdrawal rats was examined by RT-PCR,and effects of EA at Zusanli(ST36) on the body weight and expression of ?-opioid receptor mRNA of morphine withdrawal rat were observed.Results The body weight of the EA group was increased compared with the morphine abstinence group(P

Objective To investigate the role of heat shock protein 70 (HSP70) in liver flbrosis in infants with biliary atresia (BA) and its impact on prognosis. Methods Fourty-six (46) cases of infants with BA undergoing elective Kasai surgery were selected. In the same period, 30 cases of children with choledochal cyst and 17 cases of children with portal vein cavernous transformation were selected. The expressions of HSP70 in liver tissues were detected using immunohistochemical staining. The liver flbrosis in children with BA was detected using Sirius red-saturated picric acid staining. The expressions of HSP70 proteins in different flbrotic liver tissues were detected by using double staining. All postoperative BA infants were followed up and ended at June 30, 2016.Results The proportion of high expression of HSP 70 proteins in BA infants were signiflcantly higher than that in children with choledochal cyst and vein cavernous transformation (P<0.05). Rank correlation analysis showed that the expressions of HSP70 in liver tissues were positively correlated with the degree of liver flbrosis (r=0.861,P<0.05). 15 patients died among 46 cases of BA infants. The survival rate in BA children with mild liver flbrosis was 82.4%, which was signiflcantly higher than 58.6% in the severe group (P<0.05). The survival rate in HSP70 protein low expression group was 85.0%, while in HSP70 protein high expression group was 53.8%. Kaplan-meier survival analysis showed that the median survival time in the HSP70 protein low expression group was (34.0±2.6) months, while in the HSP70 protein high expression group was (18.3±2.2) months, the difference was statistically signiflcant (χx2=4.765,P=0.029).Conclusions The expressions of HSP70 proteins in liver tissues in infants with BA were high and were positively correlated with the degree of hepatic flbrosis. It suggested the possible involvement of HSP70 in the process of liver fibrosis. The upregulated expressions of HSP70 often indicated poor prognosis in children. It could be used as determining biomarker for prognosis.

Bone marrow mesenchymal stem cells (BMSCs),which can be isolated from organs and tissues,are cell populations with high degree of plasticity,similar to hematopoietic stem cells in bone marrow,and can be in vitro cultured,induced and amplified.BMSCs are increasingly used in gene therapy,cell therapy and tissue engineering.BMSCs have been currently studied in a number of autologous transplantations,while not all BMSCs are suitable for autologous transplantation.BMSCs exhibit biological aging gradually when cultured in vitro.Biological aging can be divided into age-induced senescence and long-term passage induced senescence.Aging BMSCs demonstrate changes in biological behaviour which reduces the success rate of autologous transplantation.In this review,biological aging BMSCs are discussed in order to provide help for the transplantation of BMSCs.

BACKGROUND:Implant stability is mainly influenced by peri-implant inflammatory stimulation.
OBJECTIVE:To build a beagle model of peri-implantitis under orthodontic force and to observe the bone remodeling of the Beagle dog model.
METHODS: Micro-implants were randomly implanted into the maxilary interradicular region at the center of the mesial and distal roots of bilateral P2, P3, P4 and M1 of Beagle dogs. One side served as a loaded micro-implant with peri-implantitis under 100 g of orthodontic force at 1, 2, 3, 4 weeks of peri-implantitis, and the force lasted for 1 month. After that, the animals were kiled to prepare specimens with micro-implants. Bone-to-implant contacts were calculated and histological changes of the bone interface under continuous orthodontic force at different stages of peri-implantitis were observed under light microscope.
RESULTS AND CONCLUSION:There were a large number of inflammatory cels after micro-implants were implanted with silk thread ligation to the cervical part. Over time, inflammatory cels were gradualy diffused to the tip of micro-implant, and there were a great quantity of colagenous fibers, osteocytes and active bone remodeling. When the inflammation was diffused to the tip of micro-implant after 2 weeks of peri-implantitis, woven bones composed of newly formed trabeculae appeared, and imflammatory cels dispersed. The medulary cavity was irregular after colagen fibrils absorption, and there were 3-4 layers of osteoblasts in the bone lacunae, with active bone formation. These findings indicate that the Beagle model of pure titanium peri-implantitis under orthodontic force was successfuly built in the experiment, and bone formation became active at 2 weeks after modeling.

Aged ; Humans ; Lymphoma, T-Cell, Cutaneous ; pathology ; Skin Neoplasms ; pathology

ObjectiveTo systematically assess the efficacy of acupuncture and moxibustion plus cognitive rehabilitation training in treating post-stroke cognitive disorder.MethodA computer searched CNKI, CBM, VIP, WANFANG DATA, and Pubmed and Cochrane Library for randomized controlled trials (RCT) of acupuncture and moxibustion plus cognitive rehabilitation training for the treatment of post-stroke cognitive disorder. The reference literature in the included literature was also retrieved. Retrieval time limit for both of them was from Jan. 1st 1990 to Jan. 1st 2015. Two reviewers independently selected the trials, extracted the data and assessed the quality of methodology. Meta analysis was then performed using RevMan 5.3 software.ResultEleven trials with a total of 789 subjectswere finally included. The results of Meta analysis showed that the following aspects were better in acupuncture plus cognitive rehabilitation training than in cognitive rehabilitation training or medication alone and there were statistically significant differences: the total efficacy rate [RR=1.58, 95%CI(1.10,2.26),P=0.01], the MMSE score [MD=2.64, 95%CI(1.78, 3.50),P<0.00001], P300 latency [MD=﹣18.46, 95%CI(﹣30.51,﹣6.41),P=0.003], P300 amplitude [MD=1.23, 95%CI(0.82, 1.63),P<0.00001] and activities of daily living (ADL) [SMD=0.52, 95% CI(0.31, 0.73),P<0.00001]. Based on the results of a systematical review, thequality of evidence was assessed using GRADE system recommended classification method. The results showed that the level of evidence was low and the strength of recommendations was weak.ConclusionThe results of Meta analysis showed that the therapeuticeffect was better in acupuncture and moxibustion plus cognitive rehabilitation training than in cognitive rehabilitation training or medication alone. Because of low quality of all the original literature, high-quality, multicenter and large-sample randomized blind controlled trials are still needed for validation.

Genes, T-Cell Receptor gamma ; Humans ; Lymphomatoid Papulosis ; classification ; diagnosis ; Skin Neoplasms ; classification ; diagnosis

Hotlines ; Humans ; Influenza A Virus, H7N9 Subtype ; Influenza, Human ; prevention & control ; virology ; Internet ; Preventive Health Services ; methods