Objective To study the incidence rate and clinical features of diffuse axonal injury(DAI).Methods 68 cases of diffuse axonal injury of children were analyzed according to Cordoles's diagnosis standard.Results The features of DAI were epilepsy,deep coma,pupil change and vital signs change,with low mortality rate.Conclusion DAI is serious with high incidence rate and good outcome.

Objective To present the clinic characteristics of thymoma associated with pure red cell aplasia. Methods Between 1979 and 2004, 5 patients with thymoma associated with pure red cell aplasia were surgically treated. Results The age of the patients ranged from 43 to 68 years, mean age 54.6 years. The incidence of thymoma associated with pure red cell aplasia was 2.7% (5/185) in the contemporary series. All five patients underwent radical operation. The early results of thymomectomy were excellent, 4 cases are long-term survivals, and recurrence of PRCA was found in one patient 2 months after operation and died 31 months later. Conclusion Thymoma associated with PRCA is a rare autoimmune disease. Surgery for thymoma is still the top priority in the selection of treatment. Incretion and immunosuppressor may be considered for postoperative persistent PRCA cases.

Objective To observe ophthalmoscopic image characteristics of central serous chorioretinopathy (CSC).Methods Twenty-one eyes of the 18 patients diagnosed with CSC were enrolled in this study.The patients included 12 males (14 eyes) and six females (seven eyes).The patients ages ranged from 26 to 47 years,with a mean age of (39.1±5.4) years.There were nine patients (11 eyes) with acute CSC,seven patients (seven eyes) with chronic CSC,and two patients (three eyes) with recurrent CSC.All the patients were examined using color fundus photography including infrared (IR),autofluorescence (AF),near infrared ray-auto-fluorescence (NIR-AF),fluorescein angiography (FA) and indocyanine green angiography (ICGA) photography.The ophthalmoscopic image characteristics of CSC were comparared.Results The circular serous retinal detachments of 21 eyes were depicted in color images of the ocular fundus,which in the IR showed the hypo-fluorescence.Ten eyes displayed mottled hyperfluorescent spots associated with serous retinal detachments corresponding to the leakage points.The serous retinal detachments of 15 eyes in the AF images showed hypo-fluorescence,six eyes showed hyperfluorescence.Fourteen eyes presented with hypo-or hyper-fluorescent spots corresponding to the leakage points,seven eyes presented without abnormal fluorescence corresponding to the leakage points.In addition,three eyes with acute CSC showed many scattered hyper-fluorescent spots,which showed hypo-fluorescence in the ICGA.The serous retinal detachment of 15 eyes exhibited hypo-fluorescence in the NIR-AF images,six eyes showed hyper-fluorescence.Fourteen eyes presented with hypo-or hyper-fluorescent spots corresponding to the leakage points,seven eyes presented without abnormal fluorescence corresponding to the leakage points.Twenty-one eyes in FA identified the leakage.Eight eyes showed regional choroidal delayed filling,13 eyes exhibited regional choriocapillary dilatation during 1 5 minutes after injection of ICGA.During 1-5 minutes after injection of ICGA,six eyes showed more lesions than FA,three eyes showed obvious patchy hypo-fluorescence whereas the FA were normal.Conclusions CSC has its own characteristic fundus images in the IR,FA and NIR-A.FA is still the photographic method of choice,but ICGA can reveal lesions of the choroid in CSC.IR,FA and NIR-AF are not as good as FA and ICGA for detecting of leakage points.

AIM To study the reversal effect of erythromycin on multidrug resistance of human liver cancer cells BEL 7402. METHODS Cultivated the parental cells BEL 7402 in the presence of doxorubicin to obtain a multidrug resistant subline designated BEL 7402/ADM. The expression of P glycopro tein in BEL 7402 and BEL 7402/ADM cells was determined by using cell ELASA method. The cytotoxicity of drugs was determined by using MTT assay, and the intracellular concentration of doxorubicin in BEL 7402/ADM was determined using a spectrofluorometer. RESULTS After growing BEL 7402 cells in the presence of ADM for some time, the resulting cells (BEL 7402/ADM) became not only resistant to ADM but cross resistant to VCR and MMC, the P gp on the subline cells' surface was highly expresssed. Erythromycin, which is a lipophilic antibiotic, enhanced the anti tumor functions of ADM, VCR and MMC to BEL 7402/ADM cells and increased the intracellular concentration of ADM with no effect on the expression of the P gp in BEL 7402/ADM cells. CONCLUSUON Erythromycin can significantly reverse the multidrug resistance in BEL 7402/ADM cells, it may do so by saturating the P gp pathway to reduce the efflux of intracellular concentration of the drugs.

Purpose:To explore the development change of histopathology of the mouse forestomach neoplasm inducd by B(a)P.Methods:A part of the mice were killed each four weeks after they were given benzo(a)pyrene (concentration 5mg/ml) by lavage for four weeks, twice per week. The histopathology of the forestomach was then observed.Results:Forestomach neoplasm was noticed from the 17~(th) week in the mice. Local gland hyperplasia, arrangement disorder gradually developing into glandular abnormality hyperplasia and then transformed into early stomach cancer and finally into aggressive stomach cancer could be observed.Conclusions:Latent period of the mouse forestomach neoplasm induced by B(a)P is about three months. The development course is atrophic gastritis, atypical hyperplasia and stomach cancer.[

Objective To study the influence of carbon dioxide pneumoperitoneum on the pregnancy outcome and newborn rat malformation in pregnancy rats. Methods SD rats were randomly divided into control group (group A and group B) and the experimental group (group C; group D; group E). There were 10 rats in each group, the general situation,abortion rate, premature delivery rate,newborn rats numbers,weights and lengths and malformation rates were recorded. Results There was no malformation in newborn rats in all groups (P > 0. 05 ). Conclusion It was safe to undergo laparoscope operation in metaphase pregnancy with low CO2 pneumoperitoneum,which has no influence on abortion rate and malformation rate.

Osteoporosis has become a global public health problem, and dietary interventions may potentially be helpful in preventing this disorder.Salt ( sodium chloride) is one of the most important dietary nutrients.High sodium chloride intake may play an important role in bone metabolism.In this paper, we reviewed the effects of high sodium chlo-ride intake on bone mineral density, bone mineral content and bone biochemical markers, and analyzed the possible causes through currently available literature.Although there are a few inconsistencies results, we conclude a long-term high salt intake can reduce bone density or bone mineral content, change many biochemical markers of bone resorption, which may be caused mainly by increasing urinary calcium excretion and a low-grade metabolic acidosis.However, there are still many unclear aspects need further exploration.

Objective:To used the internationally accepted methods of CT quality assurance for testing their quality, to maintain the normal operation of CT scanner and guarantee the quality of photographic image.Methods: Toshiba Activion 16 row CT, set the scanning conditions: tube voltage 120 kv, 310 mas exposure, scan time is 0.33 s, scanning layer thickness: 10 mm, 5 mm, 1.5 mm, view(FOV) 150 mm×150 mm, 512 x 512 reconstruction matrix. We measured the following performance parameters for CT spatial resolution, low contrast resolution,uniformity and noise with Catphan500 phantom made by the phantom laboratory of U.S.A.Results: The spatial resolution, low contrast resolution, uniformity and noise of the CT are all in line with the requirements. Obtained by detecting the X-ray dose, pixel, the thickness of the above parameters are affected, but also the interaction between the above parameters simultaneously.Conclusion: By detecting the spatial resolution, low contrast resolution of medical image, we can effectively guarantee system performance, maintaining optimal image quality.

Objective: To evaluate the clinical efficacy of moxibustion plus Mecobalamin in treating diabetic peripheral neuropathy. Methods: Eighty subjects were equally allocated into combination and control groups. On the basis of the same basic treatment, the combination group was treated by moxibustion plus Mecobalamin and the control group simply by Mecobalamin. Treatment lasted 3 months. Nerve conduction velocity and symptom severity were compared between pre-treatment and post-treatment. Results: Nerve conduction velocity was improved in both combination and control groups and more obviously in the combination group. The marked effective rate, effective rate and ineffective rate were respectively 52.5%, 32.5% and 15.0% in the combination group and 35.0%, 27.5% and 37.5% in the control group. There were statistically significant differences between the two groups (P<0.01 or P<0.05). Conclusion: Based on a basic treatment, moxibustion plus Mecobalamin is superior to simple Mecobalamin in treating diabetic peripheral neuropathy.

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