Objective To probe into the nursing experience of reasonable cooperation between op-eration room nurses and anesthesiologists. Methods Strengthening preoperative propaganda and psy-chological education,paying attention to the temperature and humidity in operation room,assisting the anesthesiologists to arrange patients'body position,giving cooperation of infusion and transfusion,actively involving in rescue work.Results Surgery patients got good care,anesthesia and surgery went along smoothly. Conclusions Smooth coordination between operation room nurses and anesthesiologists has great significance on the smooth going and satisfactory results of operation.

Objective To investigate the feasibility and safety of laparoscopic myomectomy in patients with large-diameter hyste-romyoma .Methods A retrospective analysis of data from 37 patients in which the uterus were larger than 14-week gestational size and the diameter of myoma was ≥7 cm treated by laparoscopic myomectomy from January 2007 to December 2011 in our hospital was conducted .The outcome of the operation were compared with that in 53 large-diameter hysteromyoma cases by abdominal sur-gery .Results 37 patients were carried out laparoscopic surgery in which 28 cases were treated successfully by total laparoscopy , and small abdominal incision was made in 6 cases .3 of the cases converted to open surgery .The ureteral abdominal fistula was ob-served in 1 case ,which was cured by a reoperation of abdominal ureteral anastomosis .All patients were successfully cured and re-tained the uterus .The cases converted to open had no significant correlation with the patient′s age ,the fibroids size ,fibroids num-ber ,operative time and blood loss volume(P>0 .05) .Compared with the laparotomy group ,the operative time ,blood loss and post-operative morbidity were similar between groups (P>0 .05) .Postoperative recovery times after laparoscopic group were less than the control group(P<0 .05) .In the follow-up of 51 months(18 to 78) ,the laparoscopic myomectomy is equally effective with the laparotomy group in the number of cases of the postoperative normal menstruation and the postoperative spontaneous pregnancy . There were no statistically significant differences between the two groups (P>0 .05) .Conclusion Laparoscopic myomectomy in pa-tients with large-diameter hysteromyoma is safe and feasible while excellent surgical skills were required for a successful surgery .

Objective To analyze the kinds and diug resistance of bacterial.Methods 1 200 cases were detected who used the antimicrobial drugs.The drug use and drug resistance were investigated.Results 612 bacterias were isolated from the 1 200 patients,the rate was 51.0％.The top five pathogens were Pseudomonas aeruginosa,fungi,Escherichia coli,Klebsiella pneumoniae,Pseudomonas alcaligenes etc..The Cefoperazone/sulbactam and imipenem against Pseudomonas aeruginosa,Escherichia coli and Klebsiella pneumoniae had better antibacterial activity,and the cefazolin,cotrimoxazole and chloramphenicol antibacterial activity were relatively low.Conclusion The clinical use and drug resistance in different regions are unique,it should pay attention to the pathogenic examination and drug susceptibility testing,early and timely antimicrobial therapy.

The occurrence of 20 to 25 percent of ovarian cancer is associated with genetic factors.Hereditary ovarian cancer includes genetic ovarian cancer and familial ovarian cancer.The genetic characteristics of tumors and the development of gene testing technology make it possible to detect tumor-related mutational genes by testing the gene of the proband and thus identify the other family members at risk and intervene early so as to realize early prevention and detection of the cancer.The paper reviews the genetic basis and pathogenesis of hereditary ovarian cancer as well as methods of detecting mutational genes, management of the proband, problems at present, and disease prevention for high-risk individuals.It stresses that we should pay attention to hereditary ovarian cancer in clinical work, identify the proband, and make comprehensive evaluation of risks of the patients` relatives so as to provide individualized guidance and carry out the concept of precision medicine.

Objective To study the indication, efficacy, safety, anesthesia method, and complication of extracorpereal shockwave lithotripsy (ESWL) in combination with endoscopic retrograde eholangiopanereatography (ERCP) for pancreatic duct stones. Methods From November 2011 to February 2016, we observed the success rate and safety of ESWL in combination with ERCP in treatment of 33 patients with chronic pancreatitis and large pancreatic duct stones and evaluate the efficacy in 6 month and 4 years follow-up. Results All the 33 patients underwent 52 ESWL, the successful fragmentation rate was 100.00%; complete clearance was achieved in 27 patients (81.82%) under the treatment of ESWL in combination with ERCP. Their pain and steatorrhea relieved, the weight increased. Conclusions ESWL combined with ERCP is effective, safe for chronic pancreatitis with pancreatic duct stones.

A series of reforms on the teaching of analytical chemistry experiment has been carried out in accordance with the characteristics of medicine examination and the actuality,which includes the content of course,the teaching method,and the inspection method.Good results have been achieved in the aspect of development of students' comprehensive quality and practical ability.

The effective running of the teaching quality supervision system in institutions of higher learning is closely connected with the efficiency of the teaching quality supervision,thus affecting whether the teaching quality can be guaranteed and promoted or not.The paper,based on the formation of the teaching quality supervision system in institutions of higher learning,probes into the problem of how to effectively run the supervision for the purpose of promotion of teaching quality in colleges and universities.

Fecal samples were collected from 81 patients with digestive tract bleeding (including 32 cases of upper digestive tract bleeding, 6 gastric cancer, 34 lower digestive tract ulcer and 9 colorectal cancer) and 40 healthy subjects. Fecal occult blood was detected with hemoglobin and/or transferrin monoclonal immunoassay. The positive rate with hemoglobin immunoassay for upper digestive tract bleeding, gastric cancer, lower digestive tract ulcer and colorectal cancer was 46.9%, 66.7%, 32.3% and 77.8% respectively;the positive rate with transferrin imrnunoassay was 71.9%, 83.3%, 47.1% and 88.9% respectively. When two methods were combined, the positive rate increased to 87.5%, 100%, 55.9% and 100% respectively showing a higher sensitivity.

This paper presents a surgical optical navigation system with non-invasive, real-time, and positioning characteristics for open surgical procedure. The design was based on the principle of near-infrared fluorescence molecular imaging. The in vivo fluorescence excitation technology, multi-channel spectral camera technology and image fusion software technology were used. Visible and near-infrared light ring LED excitation source, multi-channel band pass filters, spectral camera 2 CCD optical sensor technology and computer systems were integrated, and, as a result, a new surgical optical navigation system was successfully developed. When the near-infrared fluorescence was injected, the system could display anatomical images of the tissue surface and near-infrared fluorescent functional images of surgical field simultaneously. The system can identify the lymphatic vessels, lymph node, tumor edge which doctor cannot find out with naked eye intra-operatively. Our research will guide effectively the surgeon to remove the tumor tissue to improve significantly the success rate of surgery. The technologies have obtained a national patent, with patent No. ZI. 2011 1 0292374. 1.
Computer Systems ; Fluorescence ; Humans ; Infrared Rays ; Lymph Nodes ; Neoplasms ; Optical Imaging ; Software ; Surgery, Computer-Assisted

The electrochemical behaviors of parathion(PT) on nano-Al2O3 film modified electrode(nano-Al2O3/GCE/CME) were investigated. It was found that the redox peak currents of PT remarkably increased and the oxidation peak potential negatively shifted at the nano-Al2O3/GCE/CME. All the experimental parameters were optimized and a simple and sensitive electroanalytical method was developed for the determination of PT. The oxidation peak current was linear with the concentration of PT over the range from 2.5×10-9 to 1.0×10-7 mol/L and 1.0×10-7 to 1.0×10-5 mol/L. A low detection limit of 1.0×10-9 mol/L was obtained for 30 s accumulation at open circuit(S/N=3). The relative standard deviation(n=10) was 3.8% for 1×10-5 mol/L PT. The proposed method was used for the quantitative analysis of PT in some vegetables and the results were satisfactory.