Objective] To observe the use of large-connecting-meridians therapy(agitate blood) for the treatment of two cases of Guillain-Barre syndrome for clinical efficacy. [Method] Two cases of Guillain-Barre syndrome differentiation apply the principlesfrom yin to yang,from yang to yin,according to the meridians order, sequentially taking acupuncture, once daily treatment, continuous treatment for 5 days, rest for two days, surrounded by a continuous course of treatment, continuous treatment of four cycles. [Results] After stomach deficiency syndrome type of patients treated, appetite and sprint of improvement,the muscle gradually was full, muscle strength increased significantly, while blood cells and cerebrospinal fluid protein level were restored to normal range;After stagnation context type of patient treated,numbness feeling relieved a little, numbness narrowed, cerebrospinal fluid protein level was reduced to normal level;two cases of Guillain-Barre syndrome after treatment achieved better results.[Conclusion]Large-connecting-meridians method can be applied not only to stroke, used in Guillain-Barre syndrome can also get good results.

Chronic obstructive pulmonary disease ( COPD) is a group of chronic airway inflammatory disease characterized by sustained airflow limitation and pulmonary function damage.Inflammation and tissue repair repeatedly result in airway remodeling.Now the mechanisms of airway remodeling remain unclear.More and more researches indicated that epithelial mesenchymal transition ( EMT) is crucial in the airway remodeling of COPD.EMT could be induced by various cell factors and signal channels in the airway epithelium, thus the airway remodeling happened.This review systematically introduces EMT and focuses on the relationship of EMT and airway remodeling of COPD and the probable mechanisms.

BACKGROUND:Scientists began to use stem cells to treat Alzheimer’s disease since human beings achieved embryonic stem cells successful y in 1998.
OBJECTIVE:To review the research progress in stem cells treatment for Alzheimer’s disease.
METHODS:An online search of CNKI and PubMed databases was performed for articles published from 1998 to 2012 (CNKI) using key words of“Alzheimer’s disease, stem cells, neural stem cells, mesenchymal stem cells, induced pluripotent stem cells”in Chinese and English, respectively. The articles were included which related to Alzheimer’s disease, neural stem cells, mesenchymal stem cells and induced pluripotent stem cells, and for articles in the same field, those published recently or in authorized journals were selected. A total of 85 articles were searched. According to inclusion and exclusion criteria, 29 were included.
RESULTS AND CONCLUSION:The possible mechanisms of Alzheimer’s disease include differentiation of neural-like cells, secretion of nutrition factor, improvement of endogenous repair, stimulation of vascular regeneration and action on inflammatory cells. This review highlighted research status of neural stem cells, mesenchymal stem cells and induced pluripotent stem cells in the treatment of Alzheimer’s disease. There were advantages and disadvantages for al of them. The low immunogenicity and oncogenicity of neural stem cells are advantages, but limited source reduces their application. Mesenchymal stem cells also have low immunogenicity and oncogenicity, but their differentiation potential is not good as embryonic stem cells. Induced pluripotent stem cells also have many unsolved problems, but they have been regarded as promising stem cells.

This paper presents a surgical optical navigation system with non-invasive, real-time, and positioning characteristics for open surgical procedure. The design was based on the principle of near-infrared fluorescence molecular imaging. The in vivo fluorescence excitation technology, multi-channel spectral camera technology and image fusion software technology were used. Visible and near-infrared light ring LED excitation source, multi-channel band pass filters, spectral camera 2 CCD optical sensor technology and computer systems were integrated, and, as a result, a new surgical optical navigation system was successfully developed. When the near-infrared fluorescence was injected, the system could display anatomical images of the tissue surface and near-infrared fluorescent functional images of surgical field simultaneously. The system can identify the lymphatic vessels, lymph node, tumor edge which doctor cannot find out with naked eye intra-operatively. Our research will guide effectively the surgeon to remove the tumor tissue to improve significantly the success rate of surgery. The technologies have obtained a national patent, with patent No. ZI. 2011 1 0292374. 1.
Computer Systems ; Fluorescence ; Humans ; Infrared Rays ; Lymph Nodes ; Neoplasms ; Optical Imaging ; Software ; Surgery, Computer-Assisted

Objective: To explore the brain levels of nitric oxide (NO) and nitric oxide synthase(NOS) in rats after sleep deprivation. Methods: NO levels and NOS activity were assayed in different brain regions of Sprague-Dawley rats after sleep deprivation by flower pot compare with normal group (CC) and tank control groups (TC). Results: NO levels and NOS activity were increased in front cortex and hippocampus after SD compared with CC and TC (P

Objective:To explore the 5-HT mechanism of stress . Methods:The method of polymerase chain reaction (PCR) was used to detect the presence of 5-HT receptor subtypes in the hippocampus,hypothalamic and midbrain of the rat after 30days of movement restrict compared with normal cage control groups (CC) . The specific oligonucleotide primers were synthesized based on the complementary DNA sequence for each of the rat 5-HT 1A and 5-HT 2A receptor subtypes . Results : The expression of 5-HT 1A receptor subtypes in each regions of the rat brain after movement restrict were signifcantly lower than that of the CC groups, and the expression of 5-HT 2A receptor subtype were signifcantly higher( P

Objective To investigate women's psychological o utcome and its effect factors before and after hysterectomy. Methods In the clinical prospective study, 83 women were investigated for the ir psychiatric status with BDI, HAMD and SCL-90 questionnaires at the time of a dmission and 3 months after hysterectomy. Results The posto perative scales of BDI, HAMD, and SCL-90 were significantly lower than the preo perative ones. The morbidity of depression was decreased from 33.7% to 10.1%. Th e changes of SCL-90 were varied among different age groups. Before hysterectomy , HAMD scales were related negatively to women's educational level, but positiv ely to chief complaints. However, after hysterectomy, HAMD scales were related p ositively to parity, having other physical diseases, but negatively related to p atient's age. Conclusion Hysterectomy is beneficial to wom en's psychiatric and somatic health. Depression occurring before and after hyst erectomy is seen more often in women younger, having more parity, less-educate d, complaining of dysmenorrhea and/or menorrhagia and having other diseases.

Objective: The objective of this study is to understand the construction of laboratory testing capacity of the Chinese Disease Control and Prevention institutions at all levels and to expound the problems and puts forward the related suggestions and countermeasures which can provide the basis for scientific and corresponding improvement of laboratory construction.Methods: The whole procedures were completed through descriptive epidemiological studies using SAS 9.2 Software and Excel 2010 for conducting statistical and trend analyses.Results: There is an increasing trend in the number and proportion of inspections in the disease control institutions at all levels in China.The number of disease control and prevention institutions` special testing laboratories increased significantly in 2012 and then the growth rate undergone a slow-down.The owning rate of equipped laboratory appliance rapidly rose in 2011 and started to level off after that.The average number of equipment in eastern region is greater than that drawn in the central and western regions.According to rate of compliance with the standard of basic laboratory projects, there is a trend in the provincial, district and county level disease control and prevention institutions where the eastern region comes first, followed by the central and then the western region closes the line.However, in general, this rate of those provincial level institutions show a state that the central region holds the highest rate, the eastern region comes second, and the western last.Conclusions: The qualities of health inspection personnel still need to be further improved in disease control and prevention institutions at all levels in the country.In the aspect of laboratory equipment possession rate, the provincial-level CDCs have greater and absolute advantage than the county-level and district level institutions.The testing projects of CDCs in the central and eastern regions of China have a relatively good level, while those tracked in the western region are relatively weak.In view of this, China should strengthen the support to western region in terms of human resource, equipment and funding.The provincial-level disease control and prevention institutions` laboratory testing and diagnosis projects are the best in balance and fairness.Improving the testing capacity is an effective approach to promote the development of disease control and prevention.Hence, the state should pay much attention to the laboratory construction works and management strategies and flows.

Objective To investigate the clinical value of bronchoalveolar lavage(BAL) in treating serious pulmonary infections from surgical therapy. Methods The clinical data of 56 patients with serious pulmonary infections from surgical treatment were retrospectively investigated. 29 patients in the fibrobronchoscopy group(FBG) received treatment of BAL, 27 patients in the non-fibrobronchoscopy group(NFBG) were used as control. Results The total efficacious rate in the FBG and NFBG was 86.21% and 59.25%, respectively(P

Objective To discuss the application value of texture analysis of conventional MRI in differential diagnosis of orbital lymphoma from inflammatory pseudotumor.Methods The conventional MRI data of 15 patients with lymphoma and 17 patients with inflammatory pseudotumor proven by pathology or clinical follow-up were retrospectively reviewed.The texture features of lesions based on axial T1WI,fat-saturated T2WI and contrast enhanced fat-saturated T1WI were extracted by manually drawn ROIs with software MaZda.The subsets of optimized texture parameters were chosen by four different methods:Fisher coefficient,probability of classification error and average correlation coefficient (POE+ ACC),mutual information measure (MI) and the combination of the above three methods (FPM),respectively.Linear discriminant analysis (LDA) and nonlinear discriminant analysis (NDA) were performed for texture classification.The texture features from the sequence with the best classification result of orbital lymphoma and inflammatory pseudotumor were compared.Results The optimal texture parameters were mainly derived from co occurrence matrix and run-length matrix on T1WI and T2WI.The optimal texture parameters were mainly derived from co-occurrence matrix and histogram on contrast enhanced T1WI.The best classification of MRI texture was obtained within T2WI with lowest classification error of 1.56％ achieved by FPM in combination with NDA.Comparing the texture parameters of orbital lymphoma and inflammatory pseudotumor on T2WI,the angular second moment and long length emphasis were significantly higher in orbital lymphoma (both P＜0.005),while the entropy and short length emphasis were significantly lower in orbital lymphoma (both P＜0.005).Conclusion It is feasible to use texture analysis on conventional MRI for the differentiation of orbital lymphoma and inflammatory pseudotumor.