Objective] To observe the use of large-connecting-meridians therapy(agitate blood) for the treatment of two cases of Guillain-Barre syndrome for clinical efficacy. [Method] Two cases of Guillain-Barre syndrome differentiation apply the principlesfrom yin to yang,from yang to yin,according to the meridians order, sequentially taking acupuncture, once daily treatment, continuous treatment for 5 days, rest for two days, surrounded by a continuous course of treatment, continuous treatment of four cycles. [Results] After stomach deficiency syndrome type of patients treated, appetite and sprint of improvement,the muscle gradually was full, muscle strength increased significantly, while blood cells and cerebrospinal fluid protein level were restored to normal range;After stagnation context type of patient treated,numbness feeling relieved a little, numbness narrowed, cerebrospinal fluid protein level was reduced to normal level;two cases of Guillain-Barre syndrome after treatment achieved better results.[Conclusion]Large-connecting-meridians method can be applied not only to stroke, used in Guillain-Barre syndrome can also get good results.

Chronic obstructive pulmonary disease ( COPD) is a group of chronic airway inflammatory disease characterized by sustained airflow limitation and pulmonary function damage.Inflammation and tissue repair repeatedly result in airway remodeling.Now the mechanisms of airway remodeling remain unclear.More and more researches indicated that epithelial mesenchymal transition ( EMT) is crucial in the airway remodeling of COPD.EMT could be induced by various cell factors and signal channels in the airway epithelium, thus the airway remodeling happened.This review systematically introduces EMT and focuses on the relationship of EMT and airway remodeling of COPD and the probable mechanisms.

BACKGROUND:Scientists began to use stem cells to treat Alzheimer’s disease since human beings achieved embryonic stem cells successful y in 1998.
OBJECTIVE:To review the research progress in stem cells treatment for Alzheimer’s disease.
METHODS:An online search of CNKI and PubMed databases was performed for articles published from 1998 to 2012 (CNKI) using key words of“Alzheimer’s disease, stem cells, neural stem cells, mesenchymal stem cells, induced pluripotent stem cells”in Chinese and English, respectively. The articles were included which related to Alzheimer’s disease, neural stem cells, mesenchymal stem cells and induced pluripotent stem cells, and for articles in the same field, those published recently or in authorized journals were selected. A total of 85 articles were searched. According to inclusion and exclusion criteria, 29 were included.
RESULTS AND CONCLUSION:The possible mechanisms of Alzheimer’s disease include differentiation of neural-like cells, secretion of nutrition factor, improvement of endogenous repair, stimulation of vascular regeneration and action on inflammatory cells. This review highlighted research status of neural stem cells, mesenchymal stem cells and induced pluripotent stem cells in the treatment of Alzheimer’s disease. There were advantages and disadvantages for al of them. The low immunogenicity and oncogenicity of neural stem cells are advantages, but limited source reduces their application. Mesenchymal stem cells also have low immunogenicity and oncogenicity, but their differentiation potential is not good as embryonic stem cells. Induced pluripotent stem cells also have many unsolved problems, but they have been regarded as promising stem cells.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Female ; Humans ; Joints ; injuries ; Male ; Meridians ; Middle Aged ; Sprains and Strains ; therapy ; Young Adult

This paper presents a surgical optical navigation system with non-invasive, real-time, and positioning characteristics for open surgical procedure. The design was based on the principle of near-infrared fluorescence molecular imaging. The in vivo fluorescence excitation technology, multi-channel spectral camera technology and image fusion software technology were used. Visible and near-infrared light ring LED excitation source, multi-channel band pass filters, spectral camera 2 CCD optical sensor technology and computer systems were integrated, and, as a result, a new surgical optical navigation system was successfully developed. When the near-infrared fluorescence was injected, the system could display anatomical images of the tissue surface and near-infrared fluorescent functional images of surgical field simultaneously. The system can identify the lymphatic vessels, lymph node, tumor edge which doctor cannot find out with naked eye intra-operatively. Our research will guide effectively the surgeon to remove the tumor tissue to improve significantly the success rate of surgery. The technologies have obtained a national patent, with patent No. ZI. 2011 1 0292374. 1.
Computer Systems ; Fluorescence ; Humans ; Infrared Rays ; Lymph Nodes ; Neoplasms ; Optical Imaging ; Software ; Surgery, Computer-Assisted

Objective To investigate the long-term programming effects on pubertal reproductive function by immunological challenge in early life. Methods Female Sprague-Dawley rats were administered by endotoxin (lipopolysaccharide, LPS) at a dosage of 50 μg/kg and saline intraperitoneally on postnatal day 3 and 5. Body weight was measured weekly. Puberty onset ( vaginal opening) and oestrous cyclicity were monitored from postnatal day 30. At the age of 6 weeks, bilateral ovariectomy was performed. The histological and morphological change of the ovaries (the thickness of the theca interna and the number of different kinds of follicles) were observed and the immunoreactivity of the ovarian sympathetic nerve markers (low affinity receptor of nerve growth factor, p75NGFR) was evaluated by immune staining. Results Immunological challenge (exposed to LPS) in early life delayed vaginal opening significantly [LPS-treated (40.6 ±0.7) days versus controls ( 38. 6 ± 0. 5 ) days, P ＜ 0. 05], decreased the percentage of normal oestrous cyclicity ( LPS-treated 26. 1% versus controls 66. 8% , P＜ 0. 05 ) , decreased the total number of different types of follicles (primordial follicles: LPS-treated 610 ±47 versus controls 1181 ±57, P ＜ 0. 05; primary follicles: LPS-treated 624 ±41 versus controls 960 ± 30, P ＜ 0. 05 ; preantral follicles: LPS-treated 183 ± 16 versus controls 260 ± 14, P ＜ 0. 05; antral follicles: LPS-treated 32 ± 4 versus controls 79 ± 7, P ＜ 0. 05) and increased the thickness of the theca interna [LPS-treated ( 15. 8 ±0. 4) μm versus controls (11.4 ±0. 3) μm, P ＜ 0. 05]. The immunostaining of p75NGFR was obviously enhanced in the LPS-treated ovaries when compared with that of controls ( P ＜ 0. 05 ) . Conclusions Immunological stress during early critical developmental windows could have long dysfunctional effects on the pubertal reproductive function. It delayed puberty onset, reduced the percentage of the normal oestrous cycles, decreased follicles reserve and increased the thickness of the theca interna which might involve the up-regulation of the local ovarian sympathetic nerve activity.

Objective To investigate the clinical value of bronchoalveolar lavage(BAL) in treating serious pulmonary infections from surgical therapy. Methods The clinical data of 56 patients with serious pulmonary infections from surgical treatment were retrospectively investigated. 29 patients in the fibrobronchoscopy group(FBG) received treatment of BAL, 27 patients in the non-fibrobronchoscopy group(NFBG) were used as control. Results The total efficacious rate in the FBG and NFBG was 86.21% and 59.25%, respectively(P

Objective Clinical efficacies of angiogenesis and improvement in lower limb ischemia are compared for the transplantation of both the peripheral blood stem cells(PBSC)and bone marrow stem cells(BMSC).Methods A total of 170 patients suffering from lower limb ischemia who received stem cell transplantation were monitored for random comparison study during September 2004 to October 2006.Among them 94 patients were treated with PBSC and 76 were BMSC.All the clinical data and laboratory findings before and after the transplantation in the 1st.12th and 24th weeks were comprehensively evaluated and tested by SPSS 12.0 software package.Results The total number of MNC,CD34+ cells and CD133+ cells in PBSC group was greatly more than BMSC group and had prominent or very prominent variances(P

Objective To discuss the application value of texture analysis of conventional MRI in differential diagnosis of orbital lymphoma from inflammatory pseudotumor.Methods The conventional MRI data of 15 patients with lymphoma and 17 patients with inflammatory pseudotumor proven by pathology or clinical follow-up were retrospectively reviewed.The texture features of lesions based on axial T1WI,fat-saturated T2WI and contrast enhanced fat-saturated T1WI were extracted by manually drawn ROIs with software MaZda.The subsets of optimized texture parameters were chosen by four different methods:Fisher coefficient,probability of classification error and average correlation coefficient (POE+ ACC),mutual information measure (MI) and the combination of the above three methods (FPM),respectively.Linear discriminant analysis (LDA) and nonlinear discriminant analysis (NDA) were performed for texture classification.The texture features from the sequence with the best classification result of orbital lymphoma and inflammatory pseudotumor were compared.Results The optimal texture parameters were mainly derived from co occurrence matrix and run-length matrix on T1WI and T2WI.The optimal texture parameters were mainly derived from co-occurrence matrix and histogram on contrast enhanced T1WI.The best classification of MRI texture was obtained within T2WI with lowest classification error of 1.56％ achieved by FPM in combination with NDA.Comparing the texture parameters of orbital lymphoma and inflammatory pseudotumor on T2WI,the angular second moment and long length emphasis were significantly higher in orbital lymphoma (both P＜0.005),while the entropy and short length emphasis were significantly lower in orbital lymphoma (both P＜0.005).Conclusion It is feasible to use texture analysis on conventional MRI for the differentiation of orbital lymphoma and inflammatory pseudotumor.

Duodenal varices are an uncommon disease which occurs along the entire gastrointestinal tract outside the esophagus or stomach.Among ectopic varices,duodenal varices are the most common one.Cirrhosis,hepatic portal hypertension,extrahepatic portal vein occlusion and vasculitis contribute to the pathogenesis of ectopic variceal formation.The unusual pathophysiology,location,diversity and uncertain curative effect of DV are associated with its low detection rate and high mortality of patients.Therefore,the diagnosis and treatment of DV is one of the tough problems in clinical practice.This article reviews the current research advances in pathophysiology,diagnosis and treatment of duodenal varices to provide reference for clinical practice.