Objective To evaluate the effects of curcumin on matrix metalloproteinase-9 (MMP-9)/tissure inhibitor of metalloproteinase-1 (TIMP-1) balance during limb ischemia-reperfusion (I/R)-induced lung injury in rats.Methods Twenty-four adult male Sprague-Dawley rats,aged 6-8 months,weighing 250-300 g,were randomly divided into 3 groups (n =8 each) using a random number table:sham operation group (group S) ; group I/R; curcumin + I/R group (group C + I/R).Limb ischemia was induced by occlusion of bilateral femoral arteries for 2 h followed by reperfusion for 3 h in I/R and C + I/R groups.At 2 h before ischemia,curcumin 200 mg/kg was injected intraperitoneally in group C + I/R,while the equal volume of normal saline was given instead in the other groups.Arterial blood samples were taken at 3 h of reperfusion for blood gas analysis and PaO2 was recorded.The animals were then sacrificed and the lungs were removed immediately for determination of wet/dry lung weight (W/D) ratio,myeloperoxidase (MPO) activity and MMP-9 and TIMP-1 contents.The ratio of MMP-9/ TIMP-1 was calculated.Results Compared with group S,PaO2 was significantly decreased,and W/D ratio,MPO activity,MMP-9 content and the ratio of MMP-9/TIMP-1 were significantly increased (P ＜ 0.05),while no significant change was found in TIMP-1 content in group I/R (P ＞ 0.05).Compared with group I/R,PaO2 and TIMP-1 content were significantly increased,and W/D ratio,MPO activity,MMP-9 content and the ratio of MMP-9/TIMP-1 were significantly decreased in group C + I/R (P ＜ 0.05).Conclusion The mechanism by which curcumin attenuates lung injury induced by limb I/R is related to decreased MMP-9 content,elevated TIMP-1 content and regulated balance between MMP-9 and TIMP-1 in the lung tissues of rats.

OBJECTIVE: To improve recognization of clinical, imaging and pathological characteristics of basal cell adenoma (BCA) of parotid gland. METHOD: We collected and analyzed the data of the clinical manifestation, imaging features, histological and immunohistochemical characteristics of BCA of parotid gland (n = 9). RESULTS: Among 9 patients, 2 male, 7 female, and the average age was 55.2 (from 34 to 66 years). The clinical manifestation showed the painless mass in the parotid region. Seven cases underwent CT and other 2 cases underwent MRI. Imaging showed all tumors were located in the superficial lobe, roughly spherical and non-lobulated in shape, with well-defined boundary. The maximal diameter was less than 30 mm. Seven cases showed slightly high density shadow of soft tissue on plain CT. Compared with the parotid signal intensity, two cases showed hypointensity on T1WI and high signal on T2WI. All lesions showed obvious enhancement on delayed contrast CT or MRI. Cystic changes occurred in two cases. Gross observation: the tumors were solid in section, grey, medium in nature and clear with the surrounding tissues. Cystic changes occurred in the superficial region of superficial lobe of two cases. The capsule of tumor was well circumscribed in 8 cases and focally involved in another case. Microscopy: The parenchyma of BCA was mainly composed of basaloid cells, with myoepithelial cells palisading at the periphery of the epithelial nests. Basal membrane separated the parenchyma from the stromal, the latter lacking the myxochondroid matrix. Immunohistochemistry: basaloid cells were positive for CKpan, CD117 and CKL, while myoepithelial cells were positive for P63, SMA, and calponin. The Ki-67 lablel ing index of tumour cell was 0-4%. All patients performed superficial lobe parotidectomy and tumor dissection, and they recovered well postoperatively without tumor recurrence or malignancy. CONCLUSION The BCA of parotid gland has distinctive imaging and pathological features with favourable postoperative prognosis.
Adenoma ; pathology ; surgery ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Parotid Neoplasms ; pathology ; surgery

The electrochemical behaviors of parathion(PT) on nano-Al2O3 film modified electrode(nano-Al2O3/GCE/CME) were investigated. It was found that the redox peak currents of PT remarkably increased and the oxidation peak potential negatively shifted at the nano-Al2O3/GCE/CME. All the experimental parameters were optimized and a simple and sensitive electroanalytical method was developed for the determination of PT. The oxidation peak current was linear with the concentration of PT over the range from 2.5×10-9 to 1.0×10-7 mol/L and 1.0×10-7 to 1.0×10-5 mol/L. A low detection limit of 1.0×10-9 mol/L was obtained for 30 s accumulation at open circuit(S/N=3). The relative standard deviation(n=10) was 3.8% for 1×10-5 mol/L PT. The proposed method was used for the quantitative analysis of PT in some vegetables and the results were satisfactory.

Objective To investigate the distribution and antibiotic susceptibilities of quantitatively cultivated bacteria from bronchoalveolar lavage fluid (BALF) samples. Methods Totally 312 BALF samples were streak inoculated to chocolate,blood and MAC plates with 10 μL annulus,and the bacterial colony ＞ 104 CFU/mL was considered pathogenic bacteria. The identification of pathogenic bacteria was carried out with Vitek 2-Compact,and Kirby-Bauer disc agar diffusion method,Etest and dilution method were used for antibiotics sensitivity test.Results Totally 216 (69.2％) strains of pathogenic bacteria were isolated.The major gram-negative strains were Pseudomonas aeruginosa,Acinetobacter baumannii,Klebsiella pneumoniae and Escherichia coli, and the major gram-positive strains were Staphylococcus aureus,Streptococcus pneumoniae and Staphylococcus epidermidis.The resistance rate of Pseudomonas aeruginosa to aztreonam was high,but lower than 30％ to piperacillin/tazobactam,imipenem,cefepime,ofloxacin,ceftazidime and amikacin.Staphylococcus aureus was highly resistant to erythromycin,benzylpenicillin and clindamycin,but it was sensitive to furadantin,vancomycin,quinupristin/dalfoprisdn,tigecycline and linezolid.Conclusion The positive rate of BALF cultivation is high,and the main pathogenic bacteria Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli are resistant to several commonly used antibiotics.

0.05). Conclusion Tei index may be useful, convenient and reliable in fetal cardiac function assessment.

Objective To explore the value of high frequency color Doppler ultrasound for the early diagnosis of asymptomatic hyperuricemia in patients with joint disease.Methods Blood pressure,uric acid,total cholesterol,triglyceride and blood glucose were measured in 44 asymptomatic individual with hyperuricemia (the AH group) and 52 healthy persons (the control group),and the joints of the first metatarsalphalan geal,ankles,knees,wrists,elbows and the first metacarpophalangeal joints in the two groups were examined by high frequency and color Doppler ultrasound.T test,Chi-square test,and Fisher exact test were used for statistical analysis.Results ① The incidence rate of hypertension,diabetes and hyperlipidemia in the AH group [43.2％(19/44),22.7％(10/44),13.6％(6/44)] was significantly higher than the control group (x2=27.995,13.129,7.564;P＜0.01);② Double contour sign were found in the first metatarsal-phalangeal,ankles,knees and elbows of the AH group,in contrast to none in the control group (P＜0.05).The incidence of synovial thickening in the first metatarsal-phalangeal [1.5％(8/528)],ankles of the AH group [1.3％(7/528)]was significantly higher than that of the control group [0(0/624),0.2％(1/624)] (x2=9.521,P＜0.01;x2=5.634,P＜0.05).Similar results were found in the two groups in bone erosion in knees [1.7％(9/528) vs 0.3％(2/624)],in knees effusion [6.0％(32/528) vs 0.96％(6/624)] (x2=5.793,P＜0.05;x2=23.623,P＜0.01).The tophi was found in the fight first metatarsal-phalangeal,bilateral knees and the left first metacarpophalangeal 4 joints in 528 joints of the AH group,in contrast to none in the control group (P＜0.05).The difference in hyperechoic spots was no statistically significant between the two groups (P＞0.05).Conclusion Hyperuricemia is related to hypertension,diabetes and high blood lipids.There are some similar specific sonographic findings of gouty arthritis in the asymptomatic individuals with hyperuricemia.Therefore,these findings support that frequent ultrasound examination is a useful tool for the early diagnosis of asymptomatic hyperuricemia with joint disease,especially the screening of the first meta-tarso-phalangeal joint,ankle joint and knee joint.

Duodenal varices are an uncommon disease which occurs along the entire gastrointestinal tract outside the esophagus or stomach.Among ectopic varices,duodenal varices are the most common one.Cirrhosis,hepatic portal hypertension,extrahepatic portal vein occlusion and vasculitis contribute to the pathogenesis of ectopic variceal formation.The unusual pathophysiology,location,diversity and uncertain curative effect of DV are associated with its low detection rate and high mortality of patients.Therefore,the diagnosis and treatment of DV is one of the tough problems in clinical practice.This article reviews the current research advances in pathophysiology,diagnosis and treatment of duodenal varices to provide reference for clinical practice.

Objective To assess the safety and effica cy of local lidocaine anesthesia for pain control during transrectal ultrasound-g uided 10-core biopsy of the prostate. Methods Transrect al ultrasound-guided prostate biopsy was performed for 150 consecutive men beca use of an abnormal digital rectal examination and(or) abnormal transrectal ult rasound scan and(or) elevated prostate specific antigen.100 paients were rando mly assigned to receive an injection of 2% lidocaine (group 1,50 cases)or norm al saline(group 2,50 cases)into the lateral and apical periprostatic region,3 ml in each point,the other 50 patients(group 3) served as controls.Immediate ly after biopsy the pain score was recorded independently by a nurse using 10-p oint visual analog scale(VAS). Results The pain score range was 0 to 7.5 in the group 1,0 to 9.1 in the group 2 and 0 to 7.4 in the co ntrol group,the mean pain scores being 2.0 in the group 1,4.0 in the group 2 a nd 3.7 in the control group.The diffrence between group 1 and group 2、3 was sig nificant( P

0.05 ). Conclusions Lateral and apical periprostatic anesthesia can significantly diminish the pain and discomfort in those undergoing transrectal prostate biopsy without increasing the incidence of complications.We recommend that this procedure be routinely applied to all patients undergoing transrectal prostate biopsy.

Objective The purpose of this study was to observe the feature of tunnel enlargement after anterior cruciate ligament reconstructions and to investigate the influencing factors and the relationship between bone tunnel enlargement and clinical outcome. Methods There were 103 patients who accepted anterior cruciate ligament reconstructions with autogenous hamstring tendons for follow-up. Magnetic resonance imaging(MRI) offered the possibility of visualizing the bone tunnels from 12 to 18 months after surgery. The width was measured for tibial and femoral tunnel and compared to initial width. The tunnel size was graded to 4 grade depending on the increased width: grade 0(