Objective To investigate the correlation between the anti-nuclear antibody(ANA)pattern and the specific ANA spectrum.Methods 974 cases of detected ANA results in our hospital were analyzed retrospectively.The ANA pattern was detec-ted by the indirect immunofluorescence(IIF)and the specific ANA spectrum was tested by the line immunoassay(LIA).199 cases of both simultaneous positive results by IIF and LIA were analyzed and the correlation between ANA patterns and specific ANA spectrum was analyzed.Results Among 974 cases of specimen,249 cases(25.6%)were positive by IIF and 237 cases(24.3%) were positive by LIA,the difference in the positive rate between IIF and LIA had no statistical significance(P >0.05 ).287 cases (29.5%)were positive by single IIF or LIA or both IIF and LIA,which was higher than the positive rate by single IIF or LIA (P <0.05).Among 249 cases of positive by IIF,119 cases(79.9%)were positive by LIA,among 725 cases of negative by IIF,687 (94.8%)cases were negative by LIA.The anti-Ro-52 antibody was frequently detected in the nuclear speckled pattern,and the an-ti-M2 antibody was detected more frequently in the cytoplasmic speckled pattern,anti-dsDNA antibody and anti-nucleosome anti-body were more frequently detected in the nuclear homogeneous pattern,anti-centromere antibody was more frequently detected in the centromere pattern and anti-PM-Scl antibody was more frequently detected in the nucleolus pattern.Conclusion The ANA de-tection has better correlation between IIF and LIA,but also has certain difference.Their combined detection can reduce the missed detection rate and has the important significance to the diagnosis,disease condition monitoring and prognosis judgment of autoim-mune diseases.

Objective To compare the performance of high‐throughput ELISA and ECLIA in the determination of carcinoembry‐onic antigen (CEA) .Methods The CEA concentration of serum samples were respectively determinate by high‐throughput ELISA and ECLIA ,and the results were compared .Results Two kinds of detection methods could both accurately reflect the concentration of serum CEA .There was no significant difference between the results of two methods (P> 0 .05) .Within the linear range ,the CEA result of high‐throughput was correlate closely with that of ECLIA (r=0 .922 8 ,P<0 .01) .Conclusion High‐throughput ELISA can accurately detect the serum CEA concentration .

Objective To explore the levels of blood N-terminal pro-brain natriuretic peptide(NT-proBNP) and homocysteine(HCY) and their clinical significance in the patients with essential hypertension(EH) complicating left ventricular hypertrophy(LVH).Methods One hundred and fifty cases of HE were divided into the EH-LVH group and simple EH group according to the results of cardiac ultrasound.One hundred individuals undergoing physical examination were selected as the control group.The levels of blood NT-proBNP and HCY were compared among three groups and the correlation analysis were analyzed with LV was performed.Results The levels of blood NT-proBNP and HCY in the EH-LVH group were obviously higher than those in the EH group and control group,the EH group was significantly higher than the control group,the difference was statistically significant(P<0.05).The levels of blood NT-proBNP and HCY had significantly positive correlation with left ventricular mass index(LVMI),while negative correlation with mitral flow velocity E/A(P<0.05).Conclusion The NT-proBNP and HCY levels can be used as the indicators for monitoring the development and progress of EH and has some predictive value for its complicating LVH.

Objective To investigate the expression of interleukin-35 (IL-35) in serum of mice with pulmonary interstitial fibrosis.Methods Thirty-six C57BL/6 mice were divided into three groups (twelve mice in each group):control group,model group of mice with bleomycin-induced pulmonary fibrosis,glucocorticoid treatment group of mice with bleomycin-induced pulmonary fibrosis.The mice were sacrificed at day 7,day 14 and day 28 respectively,and the serum concentration of IL-35 was assayed.Statistical product and service solutions (SPSS) 17.0 statistical software was used for single factor analysis of variance and LSD-t comparison and Pearson correlation analysis was used for the comparison between each two groups.Results The serum IL-35 concentrations between groups and within groups at the time of day 7,day 14 and day 28 were compared respectively.The serum IL-35 concentration of the model group was significantly lower than the control group and the glucocorticoid treatment group at the time of day 7 (F=24.56,P＜0.05).The serum IL-35 concentration of glucocorticoid treatment group was significantly lower than the control group at the time of day 14 (F=8.85,P＜0.05),and which of glucocorticoid treatment group was significantly lower than the control group and the model group at the time of day 28 (F=36.64,P＜0.05).There was no significant difference between days 28 and day 7 within control group (t=1.03,P＞0.05).The serum IL-35 concentration of the model group at the time of day 28 was significantly higher than those of day 7 [(9.36±0.95) ng/ml vs (6.51±0.58) ng/ml,t=5.14,P＜0.05],and which of glucocorticoid treatment group was significantly lower than those of day 7 [(5.27±1.01) ng/ml vs (9.42±0.84) ng/ml,t=6.32,P＜0.05].From day 7 to day 28 the serum IL-35 concentration change of the model group and glucocorticoid treatment group showed significantly negative correlation (r =-0.743,P＜0.05).Conclusion Serum IL-35 concentration has shown an trend of increase during bleomycin-induced pulmonary fibrosis with mice model,and early glucocorticoid treatment can decrease the serum IL-35 in the model mice.

Objective To explore the MR imaging of reverse and separation of meniscal articular capsule.Methods MR imaging of reverse and separation of meniscus articular capsule confirmed by surgery and arthroscope were analyzed retrospectively in 8 cases.Results The “Butterfly knot sign” disappeared and was replaced with fluid signal on the sagittal slice of meniscal body in 8 cases. Part of back angle remained in 3 cases. “Double anterior cruciate ligament sign” was showed on one side of middle sagittal slice in 7 cases. “Reverse meniscus sign” was revealed in intercondylar fossa on the coronary view in ~8 cases. Abnormal high signal was showed in the injured meniscus in 6 cases. Abnormal high signal was detected in the opposite meniscus in 5 cases.Conclusion The MR findings of reverse and separation of meniscus articular capsule include disappearance of “butterfly knot sign”, appearance of “reverse meniscus sign” and “double anterior cruciate ligament sign”. The diagnosis would be established if the former 2 signs were present or all the 3 signs were present simultaneously.

Objective To analyze the imaging features and its value in diagnosing primary malignant fibrous histiocytoma(MFH) of bone.Methods Imaging features(X-ray,CT,MRI) in 8 cases of MFH confirmed pathologically,and were retrospectively reviewed.Results The location of lesions in 6 cases was in upper femur,2 cases was in upper tibilar.Worm-eaten-like bone destruction withindistinct margin was presented on X-ray film,some lesions showed slightly marginal sclerosis.Tumors were soft tissue density on CT(CT value was 34~35 HU).The masses of soft tissue were showed much distinct.Conclusion The primary MFH of bone is not of characteristic imaging findings.It can improve obviously the diagnosis of the disease by the comprehensive analysis of the imaging data.

Objective To adopt accurate and prompt nursing measures to prevent incidence of pressure through evaluation of risk factors of pressure ulcer during perioperation of thoracotomy, in order to increase nursing quality and life quality of patients. Methods The incidence of pressure ulcer in 100 patients after thoracotomy and its related risk factors were investigated by application of pressure ulcer risk factors evaluation scale, and the influence of related factors on incidence of pressure ulcer was com -pared. Results Single factor analysis showed that preoperative smoking, albumin content and prolonged postoperative turning over time had significant influence on incidence of pressure ulcer. Conclusions Patients after thoracotomy are high risk population of pressure ulcer. The pivotal period for ulcer prevention is from the operation day to 3 days after operation.

Objective To establish the dose-effect curves of chromosome aberrations in human peripheral blood lymphocytes induced by different LET rays,including 60Co γ-rays,252Cf neutrons,14MeV neutrons and 12C heavy ions at low doses,respectively,in order to assess radiation-induced damages after occupational and accidental exposure.Methods Heparinized whole blood samples were irradiated with the various radiation devices and doses ranged from 0 to 1 Gy with the interval of 0.25 Gy.Conventional chromosome culture method was applied with adding colchicines at the beginning and chromosome specimens were prepared after 48 h incubation.The Metafer scanning system was used for automatical finder of chromosome metaphases.The mathematic models were fitted according to aberration data obtained.Results At the dose range of 0 - 1 Gy,the math models of dic + r were linear for 60 Co γrays and linear-quadratics for 252 Cf neutrons,14 MeV neutrons and 12 C heavy ions.The models of ace were linear-quadratic for 60Co γ-rays and 12C heavy ion beams,and linear for 252Cf and 14 MeV neutrons.The models of total aberrations were linear-quadratic for all types of radiation.Conclusions High LET rays have higher biological effects in inducing chromosome aberrations yields compared with low LET rays.Moreover,the severity of damage is 252Cf ＞ 14 MeV neutrons ＞ 12C heavy ions ＞ 60Co γ-rays in turn.Therefore,in the range of low doses,the dic + r may be a better target of radiation damage for high LET radiation.

Objective To explore MRI findings of intervertebral suppurative spondylitis. Methods MRI findings of intervertebral suppurative spondylitis in 12 cases proved by surgery and 6 cases defined by clinical features were retrospectively analyzed. The MRI protocol included un-enhanced conventional scan in 18 cases and contrast-enhanced scan in 11 cases. Results Of the 18 cases, single focus was found in 16 cases, and multiple loci were seen in 2 cases. MRI findings included (1) Disappearance sign of nuclear crevice in 17 cases, accumulated fluid sign of intervertebral disc in 15 cases, intervertebral disc perforation in 4 cases, and intervertebral space narrowing in 7 cases. (2) Bone destruction under end plate and marrow oedema were shown in 18 cases, 17 cases had end plate destruction, 16 cases had covered sign of end plate.(3) Paraspinal soft tissue swelling was shown in 18 cases, in which thick wall microabscess was formed in 4 cases. (4) Vertebral canal was involved in 12 cases, vertebral canal abscess was formed in 5 cases.(5) Lump enhancement was demonstrated in 4 cases, nodular enhancement in 2, and ring-like enhancement in 2, respectively. No enhancement was seen in 3 cases. Dural sac linear enhancement was shown in 6 cases, and patchy enhancement in the anterior dural sac was shown in 10 cases. Conclusion Intervertebral suppurative spondytitis had characteristic MRI findings, and the key to correct diagnosis was to combine MRI finding with clinical characteristics.

Connexin43 has been shown to play a pivotal role in wound healing process. Wound repair is enhanced by acute downregulation of connexin43, by increasing proliferation and migration of keratinocyte and fibroblast. Angiogenesis is also a central feature of wound repair, but little is known about the effects of connexin43 modulation on functions of endothelial cells. We used connexin43 specific small interference RNA (siRNA) to reduce the expression of connexin43 in human umbilical vein endothelial cell (HUVEC), and investigated the effects of connexin43 downregulation on intercellular communication, viability, proliferation, migration and angiogenic activity of HUVEC. Treatment of siRNA markedly reduced the expression of connexin43 by -80% in HUVEC (P < 0.05), and decreased the intercellular communication by -65% (P < 0.05). The viability, proliferation, migration and angiogenic activity of HUVEC decreased significantly (P < 0.05), compared with that of the normal cells. The results suggest that temporally downregulation of connexin43 expression at early stage of wound to inhibit the abnormal angiogenesis characterized with leaky and inflamed blood vessels, maybe a prerequisite for coordinated normal healing process.