Surgery is an important way to treat patients with primary hyperparathyroidism.Recently,conventional bilateral neck exploration is substituted with minimal invasive surgeries gradually due to the development of the technology of pre/intro-operative localization.Video-assisted surgery is one of the minimal invasive surgeries.It includes video-assisted parathyroidectomy and total endoscopic parathyroidectomy.This article summarize the literatures of treatment of primary hyperpatathyroidism with video-assisted surgery in this article.

Objective To optimize the extraction process of Zushima Gel Cream.Methods Setting the content of daphnetin as an investigating index, and the amount of solvent, soak time and the extraction time as investigating factors, the orthogonal experiment was used to optimize the extraction process.Results The best extraction process was as follows:adding 30 mL water to degreasing Zushima Gel Cream;soaking for half an hour;extracting one time for two hours. The average content of daphnetin was 9.83 mg/g.Conclusion The extraction process is stable, reliable, and energy-saving. The study provided experimental evidence for the preparation process of Zushima Gel Cream.

AIM:To establish an effective and convenient method for applying HPLC fingerprints to quality control in the production of Shanzhuang Jiangzhi Tablet(Semen cassiae,Fructus crataegi,and Folium nelumbinis).METHODS:DiamonsilTM C18(150 mm?4.6 mm,5 ?m) analytical column was used and eluted with a gradient program consisted of phase B(methanol) and phase D(1% phosphoric acid) and detected at 254 nm;the column temperature was 30 ℃.The flow rate was 1.0 mL/min.RESULTS:Ten batches of sample tablets were tested and gained HPLC fingerprint of the tablet containing 17 common peaks.CONCLUSION:This validated method is available for quality evaluation and quality control in Shanzhuang Jiangzhi Tablet.

Objective To study the automatic word segmentation scheme suitable for traditional Chinese medical record literature. Methods Hierarchical Hidden Markov Model was used as segmentation model. Totally 300 ancient medical record literature and 300 modern medical record literature were set as experimental subjects to establish the dictionary of traditional Chinese medicine and the test corpus, with a purpose to segment the words and evaluate of the results. Results Without using dictionary of traditional Chinese medicine, the word segmentation accuracy of two kinds of medical record literature was about 75%;the part-of-speech tagging accuracy of ancient medical literature was 56.74%, the modern medical literature accuracy was 64.81%. By using dictionary of tradition Chinese medicine, the word segmentation accuracy of ancient medical record literature was 90.73%, the modern medical record literature accuracy was 95.66%;the part-of-speech tagging accuracy of ancient medical record literature was 78.47%, the modern medical record literature accuracy was 91.45%, which was obviously higher than that of ancient medical record literature. Conclusion The current word segmentation scheme has initially solved the problem of word segmentation of traditional Chinese medical record literature and part-of-speech tagging of modern medical record literature. Part of speech tagging is basically correct, but part-of-speech tagging of ancient medical record literature tagging needs further study for many influencing factors.

Clinical trials are important means to push forward the development of human health. Every new drug needs to under-take clinical trials eventually to determine the efficacy and safety. In the paper, through our clinical trial experiences and extensive lit-erature retrieval, some problems of the communication management in clinical trials were analyzed and relevant countermeasures were suggested to improve the communication management in clinical trials in order to help the development of clinical trials.

AIM:To establish an effective and convenient method for applying HPLC fingerprints to quality control in the production of Shanzhuang Jiangzhi Tablet(Semen cassiae,Fructus crataegi,and Folium nelumbinis).METHODS:Diamonsil~(TM) C_(18)(150 mm×4.6 mm,5 μm)analytical column was used and eluted with a gradient program consisted of phase B(methanol)and phase D(1 % phosphoric acid)and detected at 254 nm;the column temperature was 30℃.The flow rate was 1.0 mL/min.RESULTS:Ten batches of sample tablets were tested and gained HPLC fingerprint of the tablet containing 17 common peaks.CONCLUSION:This validated method is available for quality evaluation and quality control in Shanzhuang Jiangzhi Tablet.

OBJECTIVE:To evaluate the utilization status and trend of anti-vomiting drugs in our hospital. METHODS: The anti-vomiting drugs used in our hospital during the 2001～2006 were analyzed statistically in respect of drug kinds, dosage forms, consumption sum, DDDs etc. RESULTS: Both the drug kinds and the consumption sum of anti-vomiting drugs increased year by year in our hospital over 6 years, dominating the first place was 5-HT3 antagonists, of which, Ondansetron topped the list in consumption sum for five years and its DDDs ranked at the first place for 4 years. Domestic injections took the lead among all the anti-vomiting drugs. CONCLUSION: Consumption quantity of 5-HT3 antagonists has a rapid increase and which occupy a dominate place in our hospital.

To solve the calibration transmission problem in near-infrared ( NIR) spectroscopy, a novel model transfer method, Simple Linear Regression Direct Standard-ization ( SLRDS ) , has been presented. To investigate the validity of the proposed method, a real corn sample NIR dataset was tested and the direct standardization ( DS ) method and piecewise direct standardization ( PDS ) method were involved as a comparison. Our results indicated that SLRDS can correct compressed NIR data differences among instruments and enable the user to share corn sample PLS calibration model among three instruments, at the same time it has higher prediction accuracy, fewer parameters and simpler model than DS and PDS.

Objective:To compare the clinical outcomes between pedicle screw internal fixation via the Wiltse approach and conservative treatment in young patients with thoracolumbar fracture with Thoracolumbar Injury Classification and Severity score (TLICS) ≤ 4 points.Methods:This retrospective study included 219 young patients with thoracolumbar fracture with TLICS score ≤ 4 points who had been treated from January 2014 to December 2018 at Department of Orthopaedics, The Second Hospital of Shanxi Medical University and obtained full follow-up. They were assigned into a surgery group of 126 patients subjected to pedicle screw internal fixation via the Wiltse approach and a conservative group of 93 patients subjected to conservative treatment. The surgery group included 65 males and 61 females, aged from 18 to 37 years, with a TLICS score of 1 point in 38 cases and of 2 to 4 points in 88 ones; the conservative group included 48 males and 45 females, aged from 19 to 38 years, with a TLICS score of 1 point in 29 cases and of 2 to 4 points in 64 ones. Patients in both groups underwent thoracolumbar X-ray, CT and MRI before treatment and regular thoracolumbar X-ray reexamination after treatment. Improvements in visual analog scale (VAS) for low back pain were compared between pre- and post-treatment. The 2 groups were compared in terms of VAS, anterior height of the injured vertebra and kyphosis cobb angle between pre-treatment, one month post-treatment and the last follow-up.Results:The 2 groups were comparable due to insignificant differences between them in the pre-treatment general data ( P>0.05). In the surgery group, patients were followed up for 24 to 72 months, the average VAS scores at one month post-treatment (2.5±1.2) and the last follow-up (2.3±0.8) were significantly improved compared to the pre-treatment value (6.8±2.1) ( P<0.05), and no serious surgical complications occurred. In the conservative group, patients were followed up for 30 to 65 months, the average VAS scores at one month post-treatment (3.9±1.9) and the last follow-up (3.5±0.9) were significantly improved compared to the pre-treatment value (6.2±2.0) ( P<0.05), and the rate of complications was 11.8% (11/93, including 3 cases of neural symptoms of the lower limb, 4 cases of bedsore and 4 cases of pulmonary infection). The VAS, anterior height of the injured vertebra and kyphosis cobb angle at one month post-treatment and the last follow-up in the surgery group were all significantly better than in the conservative group ( P<0.05). Conclusion:In young patients with thoracolumbar fracture with TLICS ≤ 4 points, pedicle screw internal fixation via the Wiltse approach can lead to better therapeutic outcomes than conservative treatment, especially in relief of postoperative low back pain.

Human tumor necrosis factor-related apoptosis-inducing ligand (hTRAIL) might be developed as a novel anti-tumor drug due to its selective cytotoxicity in tumor cells. The predicted Macaca mulatta TRAIL (mmTRAIL) is highly homologous to hTRAIL in nucleotide acid as well as amino acid sequence, suggesting that mmTRAIL might induce apoptosis of human cancer cells. However, the cytotoxicity of mmTRAIL in human cancer cells has not been investigated. In this paper, it is reported that the gene encoding mmTRAIL has been cloned by using reverse-transcriptase polymerase chain reaction (RT-PCR) from monkey peripheral blood mononuclear cells (PBMCs) in our laboratory. Subsequently, an expression plasmid was constructed by inserting mmTRAIL gene into pQE30 plasmid. After induction by addition of Isopropyl β-D-1-Thiogalactopyranoside (IPTG), mmTRAIL was expressed. MmTRAIL was recovered from supernatant of sonicated bacteria by Ni-NTA agarose affinity chromatography. SDS-PAGE and gel filtration chromatography demonstrated that mmTRAIL forms trimer in solution. In vitro assays indicated that mmTRAIL was cytotoxic to human COLO205 tumor cells but not to normal cells at low concentration of nanomole. In addition, antitumor effect of mmTRAIL was evaluated in mice bearing human COLO205 tumor xenografts. Intratumorally injected mmTRAIL significantly inhibited growth of tumor grafts. These results suggested that mmTRAIL was valuable as candidate drug for cancer-targeted therapy.
Animals ; Antineoplastic Agents ; Apoptosis ; Cell Line, Tumor ; Cloning, Molecular ; Humans ; Leukocytes, Mononuclear ; Macaca mulatta ; Mice ; Plasmids ; TNF-Related Apoptosis-Inducing Ligand ; genetics ; metabolism ; Xenograft Model Antitumor Assays