Objectives To establish the mouse heart transplantation model and observe the effect of anti-rejection of IL-12 antibody.Methods Twenty-five mice undergoing heterotopic heart graft were divided into 4 groups:isograft one(n=5),simple homograft one(n=6),γ-globulin treated one(n=6)and IL-12 antibody treated one(n=8).Results The survival time of cardiac graft in the groups was as follows:＞ 100 days in isograft group,7.3 days in simple homograft group,7.5 days in r-globulin treated group and 15.4 days in IL-12 antibody treated group.Conclusions A stable mouse heart transplantation modd was established.IL-12 antibody could significantly prolong the survival of mouse cardiac graft and antagonize rejection of organ transplantation.

Objective To analyze the clinical effect of intracranial hematoma by drilling skull in treatment with hypertensive brain hemorrhage.Methods 188 patients with hypertensive cerebral hemorrhage were classified into control group with 94 cases and traditional group with 94 cases.The control group were treated with conservative medicine and observation group were treated with intracranial hematoma by drilling skull.The clinical efficacy and defection score of nerves function were observed and compared between the two groups.Results The total effective rate in observation group was significantly higher than that in control group(P＜0.05) while the death rate in observation group was significantly lower than that in control group (P＜0.05),Compared with pre-treatment,after treatment 2 weeks and 4 weeks,the Barthel index in two groups were significantly increased(P＜0.05).Moreover,after treatment 2 weeks and 4 weeks,the Barthel index in observation group was significantly higher than that in control group(P＜0.05).Conclusion The intracranial hematoma by drilling skull can enhance effective rate,decrease death rate and improve the ADL ability.

Objective To the expression of cytokines assay in peripheral blood and hematoma fluid of hypertensive cerebral hemorrhage,and the hemodynamic changes in brain tissue around the hematoma by using perfusion computed tomography imaging(PCTT)and immune turbidimetric analysis and analyze its influence on prognosis.Methods Fifty-six cases of hypertensive intracerebral hemorrhage patients were inspected with conventional CT and PCTT,quantitative analysis of hematoma and the contralateral corresponding area around the cerebral blood flow(CBF),cerebral blood volume(CBV),mean transit time (MTT),comparing acute and subacute phase CBF,CBV,MTT change.Peripheral blood,hematoma fluid compiement C_3 and C_4 levels were detected.Results In 56 patients with acute phase ipsilateral CBF,CBV,MTT were(19.3 4±3.1)ml/(min·100 g),(1.4 4±0.4)ml/g,(16.5 4±3.1)s,respectively,and in eontralateral were(50.3 4±3.4)ml/(min·100 g),(2.4±1.1)ml/g,(4.8±2.6)s,the corresponding index difference between the two sides were statistically significant(P<0.05).Subaeute phase ipsilateral CBF,CBV,MTY were(29.7±2.1)ml/(min·100 g),(1.9±0.6)ml/g,(8.9±2.7)s,the in contralateral were (48.2±6.3)ml/(min·100g),(2.3±0.7)ml/g,(4.7±1.6)s,the corresponding index difference between the two sides were statistically significant(P<0.05).Subacute phase CBF,CBV,MTT compared with acute phase,the difference Wag statistically significant(P<0.05).Acute and subacute phase of complement C_3 and C_4 in peripheral blood and hematoma fluid content of the differences were statistically significant(P<0.05).Acute and subaeute phase of edema ratios were 0.9 and 0.4,the difference was statistically significant (P<0.05).Conclusions The cerebral blood flow and cerebral perfusion of the brain tissue amund hematoma after the cerebral hemorrhage are decreased.PCTI Can analyze the intracerebral hemodynamic changes of the brain tissue around hematoma after the cerebral hemorrhage,which is helpful to treat hypertensive cerebral hemorrhage and estimate its prognosis.

Objective To investigate feasibility, efficacy and safety of holmium laser lithotripsy under ureteroscopy to treat ureteral calculi. Methods Clinical data of 162 patients with ureteral calculi treated by holmium laser lithotripsy under ureteroscopy in our hospital from June 2006 to October 2009 were reviewed retrospectively. Stone-broken rate,lithagogue rate and complication were observed. Results The overall rate of successful fragmentation for all levels of ureteral calculi in a single procedure achieved 96. 30% (156/162). The success rates of fragmentation for the upper and mid-lower segment of ureteral calculi was 86.11% (31/36)and 99.21% (125/126),respectively. In 156 patients, the postoperative follow-up of 2- 4 weeks revealed that the stone free rate was 98.08 % (153/156). The mean operative time was (36 ± 11 )mains ( ranged from 15 to 90) and hospital stay ranged from 1 to 10 days. Conclusion Holmium laser lithotripsy under ureteroscopy is feasible,high effective and safe for the treatment of ureteral calculi, especially for those with middle or lower levels ureteral calculi.

Objective To study the clinical curative effect of Azithromycin in the treatment of mycoplasma pneumonia.Methods 24 cases of mycoplasma pneumonia hospitalization patients were selected and made full analysis of their medical records.Results 24 acute patients successfully cured through Azithromycin with good clinical curative effect,beginning with symptoms of fevering,coughing,wheezing,and small blisters in lungs,accompanied by external pulmonary manifestations such as diarrhea,myocardial injury and positive results of serum Mb-IgM test.Conclusion Azithromycin in treatment of mycoplasma pneumonia could quickly retum temperature,and reduc hospitalized time.

ObjectiveTo investigate the onset of the measurability and the evolution of the subjective acuity in normal infant.MethodsTwo hundred and sixty - four normal infants were measured using the crowded HOTV recognition symbol system for subjective acuity and the closed-circuit operant preferential looking system(COPL) controlled by computer for grating acuity.The onset of measurability and the evolution of the subjective acuity of the normal infant sample were recorded and analyzed.ResultsIn the study part of the infants with 24-month old began to recognize the subjective vision symbols ( 26.32％ ).The measurability of subjective acuity of the infant group with 46 months old arrived to 100％.In the infants with 24-months old,the subjective acuity of the right eye was 4.86 ±0.05,left eye was 4.88 ± 0.08.In the infants with 46-months old the subjective acuity was 5.0 for both right and left eyes.According to Logistic regression analysis,the education background of the mother,and the instruction for infant showed the significant influencing factor.ConclusionThe study suggest that the crowded HOTV recognition symbol system controlled by computer provided a method to explore the onset of the subjective vision and the development of the infant.

Thrombospondin-1 is known as its anti-angjogenic and proapoptotic activities in oncogenesis. In current research, it has a intimate relationship with taxol resistance. Sensitivity of tumor cells to taxol will descend when the expression:of TSP1 is downregulated,and TSP1 downregulation is mediated by a new gene of taxol-resistance gene 1, the discovery provids a new trend to solve taxol resistance.

In the circumstance of that the medical system reform follows to the principle of combination of government leading and market mechanism and in view of medical ethics boundary and stratification, this paper pointed out that whether the discussion and introspection of society or the doctors,medical decision-making should focus on the relationship between medical behavior and the patients′health interests, which is ensured by reasona-ble system. Decided by the current situation of medical decision -making stratification, layered construction of medical ethics is beneficial to that the medical behaviors gradually regress to the ethical value, while establishment of diagnosis and treatment norms is the ethical choice of current medical decision-making.

Objective:To compare the effect between benazepril and candesartan combination and single medication in either one of them for treatment of hypertension-combined with microalbuminuria(MCA). Methods:A total of 95 patients were divided into three groups.Benazepril group(n=31),patients were treated with Benazepril 10～20 mg/day;Candesartan group(n=32),patients were treated with Candesartan 4～8 mg/day;and Combination group (n=32),patients were treated with benazepril 10 mg/day and candesartan 4 mg/day.The medication lasted for 8 weeks.Blood pressure(BP),plasma urinary albumin and creatinine ratio(A/C),endothelin-1(ET-1),nitrogen monoxide(NO)and potassium were examined and compared before and after the medication respectively. Results:The levels of BP,plasma A/C and ET-1 were decrease and NO was increased in all three groups(P0.05,respectively). Conclusion:Effect of benazepril and candesartan combination in patients with hypertension-combined microalbuminuria was better than single medication in either benazepril or candesartan.

Objective To study the incidence rate and clinical features of diffuse axonal injury(DAI).Methods 68 cases of diffuse axonal injury of children were analyzed according to Cordoles's diagnosis standard.Results The features of DAI were epilepsy,deep coma,pupil change and vital signs change,with low mortality rate.Conclusion DAI is serious with high incidence rate and good outcome.