Objective To the expression of cytokines assay in peripheral blood and hematoma fluid of hypertensive cerebral hemorrhage,and the hemodynamic changes in brain tissue around the hematoma by using perfusion computed tomography imaging(PCTT)and immune turbidimetric analysis and analyze its influence on prognosis.Methods Fifty-six cases of hypertensive intracerebral hemorrhage patients were inspected with conventional CT and PCTT,quantitative analysis of hematoma and the contralateral corresponding area around the cerebral blood flow(CBF),cerebral blood volume(CBV),mean transit time (MTT),comparing acute and subacute phase CBF,CBV,MTT change.Peripheral blood,hematoma fluid compiement C_3 and C_4 levels were detected.Results In 56 patients with acute phase ipsilateral CBF,CBV,MTT were(19.3 4±3.1)ml/(min·100 g),(1.4 4±0.4)ml/g,(16.5 4±3.1)s,respectively,and in eontralateral were(50.3 4±3.4)ml/(min·100 g),(2.4±1.1)ml/g,(4.8±2.6)s,the corresponding index difference between the two sides were statistically significant(P<0.05).Subaeute phase ipsilateral CBF,CBV,MTY were(29.7±2.1)ml/(min·100 g),(1.9±0.6)ml/g,(8.9±2.7)s,the in contralateral were (48.2±6.3)ml/(min·100g),(2.3±0.7)ml/g,(4.7±1.6)s,the corresponding index difference between the two sides were statistically significant(P<0.05).Subacute phase CBF,CBV,MTT compared with acute phase,the difference Wag statistically significant(P<0.05).Acute and subacute phase of complement C_3 and C_4 in peripheral blood and hematoma fluid content of the differences were statistically significant(P<0.05).Acute and subaeute phase of edema ratios were 0.9 and 0.4,the difference was statistically significant (P<0.05).Conclusions The cerebral blood flow and cerebral perfusion of the brain tissue amund hematoma after the cerebral hemorrhage are decreased.PCTI Can analyze the intracerebral hemodynamic changes of the brain tissue around hematoma after the cerebral hemorrhage,which is helpful to treat hypertensive cerebral hemorrhage and estimate its prognosis.

Objective To analyze the clinical effect of intracranial hematoma by drilling skull in treatment with hypertensive brain hemorrhage.Methods 188 patients with hypertensive cerebral hemorrhage were classified into control group with 94 cases and traditional group with 94 cases.The control group were treated with conservative medicine and observation group were treated with intracranial hematoma by drilling skull.The clinical efficacy and defection score of nerves function were observed and compared between the two groups.Results The total effective rate in observation group was significantly higher than that in control group(P＜0.05) while the death rate in observation group was significantly lower than that in control group (P＜0.05),Compared with pre-treatment,after treatment 2 weeks and 4 weeks,the Barthel index in two groups were significantly increased(P＜0.05).Moreover,after treatment 2 weeks and 4 weeks,the Barthel index in observation group was significantly higher than that in control group(P＜0.05).Conclusion The intracranial hematoma by drilling skull can enhance effective rate,decrease death rate and improve the ADL ability.

Objective To study the effectiveness of laparoscopic treatment for varicocele. Methods Clinical records of 235 cases of laparoscopic high ligation of the spermatic vein between September 1993 and July 2003 were retrospectively analyzed. Results All the operations were completed successfully. The operation time was 5~20 min (mean, 12 min). The postoperative hospital stay was 2~5 days (mean, 3 days). Follow-up observations for 6~18 months in 210 cases found 9 cases of recurrence, with a recurrence rate of 4 3% (9/210). Eleven patients with sterility had their wives pregnant 6~12 months after the surgery. Conclusions Laparoscopic high ligation for varicocele offers minimal invasion, good cosmetic results, and satisfactory effects, especially suitable for patients with bilateral varicocele, recurrent varicocele after open surgery, and surgical history in the groin.

Objective To explore the diagnosis and treatment of the fracture of posterior medial tubercle of the talus (PMTT). Methods From June 1993 to January 2002, eighteen patients were definitely diagnosed by CT scan as fractures of PMTT, but three of them had been misdiagnosed at their first visit. Of the 14 patients of them whose subtalar joint was involved, open reduction and internal fixation (ORIF) was adopted in eight, plaster cast fixation in five, and conservative management in one who had rejected any surgical procedures. Of the other four cases, plaster cast fixation of six weeks was applied in three, and excision of fracture fragments was performed in one. Results The mean follow-up was 41.7 months. Evaluation was done according to AOFAS (American Orthopedic Foot & Ankle Society) system. Satisfactory outcome was achieved in one of the first misdiagnosed patients after excision of fracture fragments. Of the others who received operative treatment, excellent result was achieved in seven cases, good in six cases and fair in three cases. The result for the one who refused surgery was poor. Conclusions The fracture of PMTT should be definitely diagnosed by CT. It should be treated operatively by means of internal fixation with half-threaded Titanium screws. Satisfactory outcome can be achieved by excision of fracture fragments for patients who were misdiagnosed at first.

Objective To investigate the relationship between procoagulation of HFGL 2 and abnormality of coagulation in patients with systemic lupus erythematosus (SLE) by detecting the expression of HFGL2/fibroleukin in peripheral blood mononuclear cell(PBMC) of SLE patients. Methods A polyclonal antibody against HFGL2 was applied to detecting the expression of HFGL2 protein in 32 SLE patients and 15 healthy volunteers by immunohistochemistry. Semi-quantitative measurement of HFGL2 expression in the blood specimen was done with high multiple image analytical system(HMIAS). Results The expression of HFGL2 in PBMC from 23 active SLE patients was significantly higher than that in the controls, which showed a positive correlation with the disease activity. Conclusion The expression of HFGL2 in PBMC is probably correlated with the pathogenesis and disease activity of SLE.

Objective: To establish the distinguish method of Jianpixiaoshi capsule.Methods: The microscopic distinguish and TLC were used. Results: 12 herbs in Jianpixiaoshi capsule can be distinguished by Microsccopic distinguish, Atractylodes macrocephala, Glycyrrhiza uralensis, Panax ginseng, Citrus reticulate, Ammomum villosum, Crataegus pinnatifida and Codonopsis pilosula can be distinguished respectively by TLC.Conclusion: The method is accurate and simple, and can be used for distinguishing Jianpixiaoshi Capsule.

Chinese materia medica (CMM) has double complexity in bioactive ingredient and its mechanism. It is difficult to explain by the modern biomedicine theory So it seriously restricts the modernization of CMM The modern CMM should have the high quality standard to meet the needs of international standard It can be guaranteed by spreading the GAP for Chinese medicinal materials and GMP for standard production The mechanism depends on using the DNA microarray to set up “the gene expression difference chart”, to study on the combination of CMM and gene expression difference chart Meanwhile, we can establish a totally new method of screening modern CMM based on the gene expression difference chart, it can really make the modernization and internationalization of CMM

Objective To study the automatic word segmentation scheme suitable for traditional Chinese medical record literature. Methods Hierarchical Hidden Markov Model was used as segmentation model. Totally 300 ancient medical record literature and 300 modern medical record literature were set as experimental subjects to establish the dictionary of traditional Chinese medicine and the test corpus, with a purpose to segment the words and evaluate of the results. Results Without using dictionary of traditional Chinese medicine, the word segmentation accuracy of two kinds of medical record literature was about 75%;the part-of-speech tagging accuracy of ancient medical literature was 56.74%, the modern medical literature accuracy was 64.81%. By using dictionary of tradition Chinese medicine, the word segmentation accuracy of ancient medical record literature was 90.73%, the modern medical record literature accuracy was 95.66%;the part-of-speech tagging accuracy of ancient medical record literature was 78.47%, the modern medical record literature accuracy was 91.45%, which was obviously higher than that of ancient medical record literature. Conclusion The current word segmentation scheme has initially solved the problem of word segmentation of traditional Chinese medical record literature and part-of-speech tagging of modern medical record literature. Part of speech tagging is basically correct, but part-of-speech tagging of ancient medical record literature tagging needs further study for many influencing factors.

BACKGROUND:Scientists began to use stem cells to treat Alzheimer’s disease since human beings achieved embryonic stem cells successful y in 1998.
OBJECTIVE:To review the research progress in stem cells treatment for Alzheimer’s disease.
METHODS:An online search of CNKI and PubMed databases was performed for articles published from 1998 to 2012 (CNKI) using key words of“Alzheimer’s disease, stem cells, neural stem cells, mesenchymal stem cells, induced pluripotent stem cells”in Chinese and English, respectively. The articles were included which related to Alzheimer’s disease, neural stem cells, mesenchymal stem cells and induced pluripotent stem cells, and for articles in the same field, those published recently or in authorized journals were selected. A total of 85 articles were searched. According to inclusion and exclusion criteria, 29 were included.
RESULTS AND CONCLUSION:The possible mechanisms of Alzheimer’s disease include differentiation of neural-like cells, secretion of nutrition factor, improvement of endogenous repair, stimulation of vascular regeneration and action on inflammatory cells. This review highlighted research status of neural stem cells, mesenchymal stem cells and induced pluripotent stem cells in the treatment of Alzheimer’s disease. There were advantages and disadvantages for al of them. The low immunogenicity and oncogenicity of neural stem cells are advantages, but limited source reduces their application. Mesenchymal stem cells also have low immunogenicity and oncogenicity, but their differentiation potential is not good as embryonic stem cells. Induced pluripotent stem cells also have many unsolved problems, but they have been regarded as promising stem cells.

Objective:To investigate a classification on age of diagnosis and its clinical characteristics in hospitalized elderly patients with first diagnosed Crohn's disease.Methods:This was retrospective case-control study.A total of 181 newly diagnosed CD patients were admitted to Huadong Hospital from January 2005 to December 2019.According to Montreal CD classification criteria based on the age at the time of diagnosis(sub-grouping A3 into A3 and A4 in this paper), 181 CD patients were classified into four groups: the A1 group(≤16 years, n=4), A2 group(17-40 years, n=60)and A3 group(41-59 years, n=63)(above three as control groups), and the A4 group[≥60 years, with elder-onset Crhon's disease(EOCD), n=54]as a study group.Results:A total of 181 first diagnosed CD patients who met the diagnostic criteria were included in this study.During the investigation period, the total incidence rate of CD was 37.90/100, 000, and the total prevalence rate of CD was 142.79/100, 000.Among the 181 first diagnosed CD patients, there were 4 patients(2.2%)with age of diagnosis of ≤16 years(group A1), 60 patients(33.2%)with age of diagnosis of 16-40 years(A2), 63 patients(34.8%)with age of diagnosis of 41-59 years(A3)and 54 patients(29.8%)with age of diagnosis of ≥=60 years(A4). Of these first diagnosed CD patients, male were dominant in A2 group(51 males / 9 females), while the ratio of males and females tended to be the same(32 males /31 females)in A3 group, and the proportion of female patients was greater than that of male patients in A4 group(25 males /29 females)( P<0.0001). The main CD lesion was ileocolic type in the EOCD group, accounting for 57.4%( P=0.0077). The incidence rate of ileus type CD was 42.6%( P=0.1942). Among the 163 CD patients who underwent colonoscopy, under Simple Endoscopic Score for CD(SES-CD)evaluation, the proportion of SES-CD severity type in EOCD group was as high as 82.2%, which was higher than that in the A3 group(51.7%, P=0.0187). All CD patients underwent pathological examination.There were 173 cases(95.6%)with focal lymphocytic infiltration, 120 cases(66.3%)with inflammatory infiltration on the crypt epithelial and 82 cases(45.3%)with non-caseous granuloma tissue.In the EOCD group, non-caseous granuloma accounted for 61.1%, which was higher than that in the A3 and A2 groups( P=0.0318). In the EOCD group, the non-complication rate was 1.89%, and the incidence rates of 3, 4 and ≥5 complications were 14.8%, 22.2% and 24.1%, respectively( P<0.0001, P=0.0280, 0.0141 and 0.0013). The sulphasalazine(SASP)alone was the main treatment method, accounting for 42.6%( P=0.0038), and the surgery accounted for 24.1%( P=0.9598). Conclusions:The incidence and trend of EOCD are basically consistent with those of adult-onset CD and showed an rising volatility.Incidence rate of EOCD is higher in females than in males.EOCD lesions of visual observation under endoscope are serious.The detection rate of non-caseous granulomas and related scars is high under microscope.EOCD patients have many complications, and SASP alone is the main treatment method.