This study was aimed to evaluate the subchronic toxicity of diosgenin in mice. A total of 80 mice were divided into 4 groups, which were 0 (control), 100, 200, and 400 mg·kg-1 by the random number table. Intragastric administration was given once a day for 90 days in the assessment of subchronic toxicity of diosgenin among mice. The observed indexes contained body weight, fur color, diet, feces, and etc. The detected indexes contained blood routine analysis, blood biochemistry and pathological examination. The results showed that compared with the control group, the body weights of mice in the male medication group were slight reduced. There were no significant hematologic and pathologic abnormalities. It was concluded that the subchronic toxicity of diosgenin with no observed adverse effect dose level was more than 400 mg·kg-1. The oral administration was relatively safe.

Objective To detect the serum levels of epidermal growth factor（EGF）in rheumatoid arthritis（RA）patients,and explore the possible role of EGF in RA.Methods The serum level of EGF was measured by enzyme-linked immunosorbent assay（ELISA）in 38 RA patients diagnosed according to dianostic criteria for RA by American Rheumatism Association and 20 healthy controls.Rheumatoid factor（RF）,erythrocyte sedimentation rate（ESR）and C-creative protein（CRP）were also measured in these 38 RA patients.The relation beween the serum level changes of EGF and the corresponding clinical indexes in the two groups was statistically analyzed.Results Compared with the healthy individuals,the serum EGF levels of RA patients increased significantly（P 0.05）.Compared with simple bone rarefaction patients,the serum levels of EGF increased significantly in the bone erosion patients（P 0.05）.The serum levels of EGF in RA patients had no correlation with RF,ESR and CRP levels（P 0.05）.Conclusion The evident increase of serum EGF level in RA patients,especially in bone erosion patients,suggests that EGF may partly play a role in the pathogenesis of RA.

Objective To evaluate the safety and effectiveness of the Hem-o-lok in retroperitoneal laparoscopic nephrectomy.Methods A total of 178 patients underwent retroperitoneal laparoscopic nephrectomy from November 2003 to November 2006.The operation was performed through 3 lumbar ports.The renal artery and veins were blocked with the Hem-o-lok and then severed.The ureter was cut at the site near the iliac blood vessel.For living donor nephrectomy,the kidney was taken out and infused with 4℃ kidney preserving fluid immediately.Results The operation was successfully completed in all the 178 cases and there was no conversion to open surgery.No Hem-o-lok ligating clips became dislodged.The mean operation time was 100 min(60-200 min),and the mean blood loss was 95 ml(20-200 ml).Complications included renal vein injury in 1 patient and pancreatic tail injury in 1 patient.The mean length of postoperative hospital stay was 5 days(4-8 days).Pathological results showed renal clear cell carcinoma in 65 patients,renal cystadenocarcinoma in 3 patients,renal leiomyosarcoma in 1 patient,renal lymphoma in 1 patient,renal oncocytoma in 2 patients,renal hamartoma in 2 patients,ureteral transitional cell carcinoma in 12 patients,ureteral squamous cell carcinoma in 2 patients,renal pelvis transitional cell carcinoma in 31 patients,renal pelvis xanthogranuloma in 1 patient,renal tuberculosis in 4 patients,accessory kidney in 1 patient,renal calculus in 4 patients,nephrohydrosis in 4 patients,and renal abscess in 1 patient.A total of 136 patients were followed for 1-36 months(mean,25 months),involving 64 patients followed for

This paper described the application of WeChat public platform and WeChat groups in hospital culture development.As proposed by the authors,WeChat can be used by the hospitals to strengthen media integration,cultivate medical personnel′s media literacy,improve the supervision mechanism of WeChat,take tide of the new media trend,and adhere to the cultural development,thus leading the reform and development of the hospital.

Objective By collecting rheumatoid arthritis (RA) periodontal parameters,analyzing the general information and clinical indicators to understand the relationship between RA and periodontitis.Methods From December 2014 to May 2016,132 cases of RA,50 cases of the periodontitis group were enrolled.Their periodontal parameters,general data and clinical indicators were analyzed and the measurement data were compared with t test.Counting data was compared by Chi square or Fisher's exact test for statistical analysis.Results The prevalence rate of periodontal disease in patients with rheumatoid arthritis was high (73％).When the RA complicated with periodontitis group and RA without periodontitis group were compared,the C-reactive protein (CRP) [(43±33) mg/L,(30±27) mg/L,t=2.213,P＜0.05],erythrocyte sedimentation rate (ESR) [(50±30) mm/1 h,(38±25) mm/1 h,t=2.263,P＜0.05],rheumatoid factor (RF) [(319±368) U/ml,(174±228) U/ml,t=2.783,P＜0.05],anti-cyclic citrullinated peptide (CCP) [(511 ±700) AU/ml,(262±429)AU/ml,t=2.535,P＜0.05],the positive rate of anti-CCP [80 (83％),17 (47％),x2=17.522,P＜0.001],health assessment questionnaire (HAQ) (2.5±0.8),(2.2±0.8),t=2.011,P＜0.05) of the RA complicated periodontitis group was statistically significantly different,but disease activity score (DAS)28 (5.5±0.7,5.3±0.7,t=1.970,P＞0.05) was not statistically significantly different.The anti-CCP positive group and anti-CCP negative group were compared in the number of patients with missing teeth [22 (23％),15 (43％),x2=5.19,P＜0.05],missing teeth number (3.5±1.5,2.5±1.4,t=2.004,P＞0.05),probingdepth (PD) (5.3±1.4) mm,(5.0±1.4) mm,t=1.094,P＞0.05),clinicalattachmentLevel (CAL) (5.2±1.4) mm,(4.9±1.5) mm,t=0.946,P＞0.05),bleedingon-probing (BOP) [17 (18％),7 (20％),x2=0.106,P＞0.05) was not statistically significant.Periodontitis with RA group and periodontitis group were compared with the number of patients with missing teeth [21(42％),5(10％),x2=4.11,P＜0.05],number of missing teeth (1.10±0.29,1.20±0.40,t=0.638,P＞0.05),PD [(4.3±1.7) mm,(3.2±1.4) mm,t=4.902,P＜0.05],CAL [(3.9±2.0) mm,(3.4±1.7) mm,t=2.18,P＜0.05],BOP [18(36％),9(18％),x2=13.306,P＜0.05] and CRP [(30±26) mg/L,(20±16) mg/L,t=3.820,P＜0.05],ESR [(45±21) mm/1 h,(32±18) mm/1 h,t=3.047,P＜0.05],was statistically significantly different.Conclusion The incidence of periodontitis in patients with RA is high;periodontitis may have a certain promoting effect on RA,and RA may also have some promoting effects on periodontitis.

BACKGROUND:Biliary stent is widely used in different biliary tract surgeries, and there are some defects in the use of biliary stent. OBJECTIVE:To explore the biodegradability and compatibility of polylactide/glycolide biliary stent. METHODS:The polylactide/glycolide biliary stent was prepared, and immersed in the bile. At 1, 2, 3, 4 and 5 weeks after soaking, eight experimental stents were dried and placed under the scanning electron microscope. In the sterile condition, the stent was implanted under the rat skin. At 1, 2, 3, 4, and 5 weeks after implantation, two rats were sacrificed. The stent was taken out. The appearance of the stent and muscle structure of experimental animals was observed. The gross appearance of stent was observed at different time by scanning electron microscopy. The rate of degradation was calculated. The muscle structure of experimental animal and the stent were understood. RESULTS AND CONCLUSION:At 1 week, stent shape remained almost intact, but the texture became soft;surface roughness and cracks gradual y appeared;stent wal col apse was visible. The stent was completely degraded at 5 weeks. Before degradation, the stent presented clear three-dimensional reticular structure observed by scanning electron microscopy. With the time extended, the stent surface and section corrosion became worsened. By gel permeation chromatography detection, at 1 week of implantation, the molecular mass of the stent declined rapidly, and then gradual y declined. At 2 weeks, the relative molecular mass decreased to 15 000. At 4 weeks, stent mass loss was approximately 40%. Al the experimental animals were survived, no poisoning, al ergy or heat source response. The operative wound was healed wel , and no infection occurred. By histological observation, at 5 weeks, the stent was completely degraded, with a degradation rate of 100%. Surrounding muscles recovered to a normal state. These results indicate that the polylactide/glycolide biliary stent has good degradation in vitro and biocompatibility.

Objective To investigate whether the new mechanically-activated (MA) cation channel Piezo 1 protein can cause the apoptosis of human chondrocytes under compressive loading,using a Flexercell unit by activating MAPK/ERK5 signal pathway.Methods Primary human chondrocytes were isolated,cultured,and then subjected to the static compressive loading for 2 h,12 h,24.h and 48 h,respectively.The GsMTx4,which is the special inhibitor of the Piezo1 protein and the BIX02188,which is the inhibitor of the ERK5 served as the inhibitor group.The immunofluorescence was used to locate the expression of the Piezo 1 protein.The expressions of Piezo1 and ERK5 were assessed by reverse transcription-polymerase chain reaction (RT-PCR),as well as the apoptosis gene B cell lymphoma/leukemia-2 (Bcl-2),Bcl-associated X protein (Bax) and Bcl-associated death promoter (BAD).In addition,Piezo1 inhibitor,GsMTx4,was used to block the MA cation channel Piezo1,served as the inhibitor group.AVPI was used to detect the apoptosis of the OA chondrocytes.Results The location of the Piezo1 was expressed in nucleus and cytoplasm of chondrocytes.The expression of the Piezo1,ERK5,BAD,and Bax mRNA in the OA chondrocytes is weak.The 12 h stretch force group was significant increased,and the 24 h stretch force group was the highest expression.However,the expression of the 48 h group was decreasing.The expression of the Bcl-2 in the 12 h group was decreasing,and the 12 h stretch force group was the lowest expression while the 24 h stretch force group was increasing.In the GsMTx4 group,the expression of the Piezo1,ERK5,BAD,Bax was decreasing while the Bcl-2 was increasing.In the BIX02188 group,the expression of the ERKS,BAD,Bax was increasing while the Bcl-2 was decreasing,while the expression of the Piezo1 was not change.The result of AV-PI shown that the 2 h stretch force group increased early stage of apoptosis.The 12 h stretch force group increased late stage of apoptosis,and the 24 h stretch force group's apoptotic rate was the highest.However,the apoptotic rate of the 48 h group was lower than the 24 h stretch force group.The GsMTx4 could inhibit the late stage of apoptosis.Conclusion Piezo 1 plays an important role in the apoptosis of human chondrocyte through the MAPK/ERK5 signal pathway.

Percutaneous endoscopic lumbar discectomy belongs to minimally invasive spine operation Its superiority includes smalleroperation wound,less bleeding,shorter hospital day,and earlier return to function,conpared with the traditional operation.At the same time,percutaneous endoscopic lumbar discectomy has complications,as the open operation.This paper reviews its common complications,diagnosis,prevention and control.

Objective A kidney transplantation patient who was diagnosed with autoimmune hemolytic anemia (AIHA) caused by passenger lymphocyte syndrome (PLS) was reviewed.Method A male kidney transplantation patient aged 31 was admitted due to severe anemia.Direct antiglobulin test (DAT) was positive and reticulocyte was elevated significantly,and PLS was diagnosed.He was treated with blood transfusion,glucocorticoid and intravenous immunoglobulin,and recovered at last.Result PLS is a rare but important cause of AIHA after kidney transplantation,often occurs in blood type A patient who received a kidney from a blood type O donor.Final diagnosis depends on the detection of anti-erythrocyte antibody in recipient serum.Conclusion PLS should be considered when anemia with unknown reasons occurred in kidney transplantation patients.

Objective The level of IL?17A in serum and gingival fluid was measured and the clinical significance was analyzed ,so as to under?stand the relationship between rheumatoid arthritis(RA)and periodontal lesions. Methods The study participants included 20 patients with RA and CP,20 patients with RA,20 patients with RA,20 patients with CP,and 20 healthy controls. All groups were balanced for age,sex,and smok?ing status. The expression levels of IL?17A in serum and gingival fluid of the four groups were determined by ELISA method. Results The level of serum IL?17A in RA+CP group,RA group,CP group and H group respectively 66.6±24.98,46.58±8.50,31.35±5.10,and 29.25±5.50 pg/mL;RA+CP group,RA group,CP group,H group of gingival fluid IL?17A levels were 25.71±5.1,19.97±4.95,18.85±5.36,and 15.38±4.36 pg/mL. The level of serum IL?17A and anti?CCP of patients in the RA+CP group had positive correlation(r=0.446,P<0.05),and the level of gingival fluid IL?17A and anti?CCP of patients in the RA+CP group had positive correlation(r=0.517,P<0.05). Compared with RA group,the visible clinical laboratory ESR,CRP,RF,anti?CCP,DAS28 and HAQ scores of RA+CP group were increased. Conclusion Rheumatoid arthritis may exacerbate the periodontal disease in patients with periodontal disease. On the other hand ,periodontal disease may also promote rheumatoid arthri?tis.