Objective To explore the clinical effect of Haizhu-Chuanxidingtablet with MORA-super bio-resonance in bronchial asthma.Methods A total of 94 patients met the inclusion criteria in our hospital were divided control group and observe group by a random way, 47 in each group. The control group was treated with MORA-super bio-resonance while the treatment group withHaizhu-Chuanxiding tablet on the base of control group. The treatment time was 50 days in the 2 groups. After the treatment, the effects in the 2 groups were compared. Before and after the treatment, the levels of lung function (FVC, PEF, FEV1% and FEV1), inflammation index (hs-CRP, IL-8,TNF-α and IL-4), matrix metalloproteinase-9 (MMP-9), tissue inhibitors of metalloproteinase 1 (TIMP-1), toll like receptor 2 (TLR2) and eosinophil cationic protein (ECP) were detected in the 2 groups.Results After the treatment, the effect rate was 91.5% (43/47) in the observe group, significantly higher than 72.3% (34/47) in the control group (Z=4.201,P=0.037). After the treatment, the levels of FVC (2.45 ± 0.37 Lvs.1.93 ± 0.32 L,t=3.561), PEF(6.16 ± 1.25 L/svs. 5.56 ± 1.10 L/s,t=3.413), FEV1/FVC (68.39% ± 6.44%vs. 62.31% ± 5.97%,t=3.315) and FEV1 (2.61 ± 0.56 Lvs. 2.21 ± 0.47 L,t=3.672) in the observe group significantly higher than the control group (P<0.05). After the treatment, the levels of hs-CRP (8.14 ± 0.42 mg/Lvs. 12.41 ± 0.65 mg/L,t=3.424), IL-8 (113.47 ± 11.13 ng/Lvs. 153.81 ± 19.73 ng/L,t=3.692), TNF-α (0.31 ± 0.04 ng/Lvs. 0.70 ± 0.13 ng/L,t=3.923)], IL-4 (39.43 ± 6.23 ng/Lvs. 47.75 ± 8.23 ng/L, t=3.783) in the observe group significantly lower than the control group (P<0.05). After the treatment, the levels of MMP-9 (84.89 ± 9.19 ng/mlvs. 113.35 ± 11.35 ng/ml,t=3.671), TIMP-1 (91.53 ± 8.78 ng/mlvs. 131.15 ± 12.45 ng/ml,t=3.782), ECP (19.43 ± 1.33 ng/Lvs. 25.75 ± 2.13 ng/L,t=3.561) in the observe group significantly lower than the control group (P<0.05). The level of TLR2 (270.31 ± 7.01 ng/Lvs. 198.70 ± 10.13 ng/L;t=3.871,P=0.038) in the observe group significantly higher than the control group (P<0.05).ConclusionsHaizhu-Chuanxidingtablet with MORA-super bio-resonance in bronchial asthma showed a good effect on patients with bronchial asthma and could promote the inflammation and lung function in patients.

Objective To evaluate the effect and the incidence of adverse reaction of different chemotherapy combined with radiotherapy for the treatment of non-small cell lung cancer (NSCLC) complicated with type 2 diabetes.Methods A total of 123 midterm advanced NSCLC patients complicated with type 2 diabetes were randomly divided into A,B,C three groups through the random number generated by computer,and each group was 41 cases.Three groups of patients were treated with three-dimensional conformal radiotherapy and synchronous chemotherapy.Group A received gemcitabine and cisplatin,group B received paclitaxel and cisplatin,and group C received pemetrexed and cisplatin.The treatment efficiency,time to tumor progression,median survival time,survival rate and incidence of adverse reaction were observed and compared.Results The total effective rates of group A,B and C were 82.93％,78.05％ and 80.49％,with no statistically significant difference (x2 =0.365,P =0.357).The times to tumor progression of the three groups were (6.54 ± 1.23) months,(5.96 ± 1.27) months,(6.27 ± 1.08) months,with no statistically significant difference (F=1.235,P =0.314);the median survival times of the three groups were (16.54 ± 1.87) months,(15.36 ± 1.25) months,(16.29 ± 2.08) months respectively,with no statistically significant difference (F =2.226,P =0.245);1-year survival rates of the three groups were 80.49％,82.93％,85.37％ respectively,with no statistically significant difference (x2 =1.234,P =0.665);2-year survival rates were 48.78％,51.22％,43.90％,with no statistically significant difference (x2 =2.354,P =0.451).The incidence rates of radioactive pneumonia and bone marrow suppression in group C were 24.39％ and 24.39％,those were significantlylower than31.71％,53.66％ of group B (x2 =18.687 P=0.017;x2 =25.336,P=0.011) and 41.46％,31.71％ of group A (x2 =17.543,P=0.019;x2 =24.358,P =0.013).Conclusion The clinical efficacy of the three groups are similar,but the incidence rate of adverse reaction in pemetrexed and cisplatin plus radiotherapy group is the lowest.Therefore,pemetrexed plus cisplatin is the safe and effective treatment for the previously untreated patients with NSCLC.

Objective To evaluate the role of lumbosacral somatosensory evoked potential (LSSEP) examination in the diagnosis of lumbosacral disc herniation (LDH). Methods Somatosensory evoked potential (SEP) from posterior tibial nerve, L_4, L_5 and S_1 dermatomes SEP, and lumbosacral SEP was recorded in 54 patients with radiculopathy caused by LDH. Results The SEP from posterior tibial nerve was abnormal in 22 cases (40.74%). The SEP from L_5 and/or S_1 dermatome was abnormal in 47 cases (87.04%, P

The research on the training program of medical education postgraduates will help to cultivate medical education talents with professional knowledge and ability, so as to further promote the specialization and sustainable development of medical education. In the practice of educational research, under the guidance of personnel training objectives, a relatively complete theoretical curriculum system has been set up around students' ideological and political quality, scientific research ability, theoretical and practical knowledge learning and application. In addition, the practical courses of postgraduate training have been rationally designed, and the evaluation methods of the effects have been planned scientifically and reasonably. Through the research, we can make the postgraduate training work of medical education has rules to follow, which plays a key role in improving the quality of education and training.

Objectives To assess the clinical value of ultrasonography (US) and bioelectrical impedance analysis (BIA) in analyzing abdominal fat contents of obese children and adolescents through comparison with MRI. A correlation with other obese related metabolic parameters was conducted. Methods Ninety 7-17-y-old obese children and adolescents (60 boys and 30 girls with mean age of 9.6 ± 2.9 y and mean BMI of 24.5 ± 4.5 kg/m2) were recruited. Metabolic parameters were measured, and insulin resistance was estimated according to homeostasis model assess-ment (HOMA-IR). On the same day abdomen subcutaneous fat thickness (SFTUS) was measured by US. Body fat mass (FMBIA) and abdominal visceral fat area (VFABIA) were analyzed by bioelectrical impedance analysis (BIA). After obtaining informed consent, abdominal MRI was performed in 20 subjects. Each section of umbilicus level was analyzed by image threshold value segmentation using SigmaScan Pro 5 and abdominal subcutaneous fat area (SFAMRI) and visceral fat area (VFAMRI) were calculated. Results (1) A strong positive association was found between SFTUS and SFAMRI (P< 0.05), VFABIA and VFAMRI (P < 0.01) respectively. (2) FMBIA and SFAMRI, VFAMRI, SFTUS also showed significant correlations (P < 0.05). (3) VFAMRI showed extremely significant positive correlations with TG, Insulin,C-peptide and HOMA-IR (P < 0.01 ) ; SFAMRI was also correlated positively with them (P < 0.05). (4) SFTUS was correlated positively with UA (uric acid), Insulin, 2HIns (insulin measured at 2 hours after meal), C-peptide,2HC-peptide (C-peptide measured at 2 hours after meal) and HOMA-IR (P < 0.01). (5) VFABIA was correlated significant positively with UA, insulin, TG, 2HIns and HOMA-IR. FMBIA showed positive correlation with UA, Insulin,2HIns, C-peptide, 2HC-peptide and HOMA-IR. Conclusions abdominal subcutaneous and visceral fat of obese children and adolescents evaluated by US and BIA are correlated well with those assessed by MRI, and also correlated well with TG, insulin, C-peptide and other metabolic biochemical parameters. Our data support the value of using cost effective, simple and convenient methods such as BIA and US to evaluate the obese and related metabolic risk of children and adolescents in clinical practice.

Objective To investigate the factors affecting pulse transit time during anesthesia. Methods Sixty cases of gastrointestinal surgery under general anesthesia were investigated.The pulse transit time (PTT),BP and HR were measured at the time before and after intravenous anes-thesia induction,and at the time before and after the injection of vasoactive drugs,respectively. Results Compared to at the time before injection,HR,SBP and DBP increased significantly,and PTT decreased significantly (P <0.01 )at the time after injection of atropine and ephedrine.Com-pared to at the time before injection,HR and PTT increased significantly,while SBP and DBP de-creased significantly (P < 0.01 )at the time after injection of nicardipine.While HR and PTT de-creased significantly,and SBP,DBP increased significantly (P <0.01 )with the injection of pheny-lephrine.Obvious negative correlation between SBP and PTT was observed before and after induc-tion;meanwhile,only weak correlation between DBP and PTT was observed,and no obvious correla-tion between HR and PTT was detected.SBP and PTT were well negatively correlated with the injec-tion of atropine,ephedrine,nicardipine or phenylephrine.Conclusion All the PTT changes during anesthesia were found to be negatively correlated with the systolic blood pressure.

Objective To discuss the treatment effect of retrograde intramedullary nails under the guide of arthroscope in treating supracondylar fractures of the femur. Methods A total of 24 su-pracondylar fractures of the femur were treated with retrograde intramedullary nails under the guide of ar-throscope. All the patients were followed up for a clinical evaluation. Results The follow up for mean 14 months in all patients showed that all fractures were healed, with mean healing duration for three months. All the patients obtained good or excellent results after operation. Conclusions In treatment of supracondylar fractures of the femur, retrograde intramedullary nail under the guide of arthroscope has the advantages of mierotrauma, accurate location, firm fixation and short fracture healing time that are beneficial to joint function recovery and is worthy of clinical application.

Objective To investigate the protective effect of peroxisome proliferator-activated receptor ?(PPAR?) activator 15-deoxyprostaglandin J2(15d-PGJ2) in rat hepatic ischemia-reperfusion injury and its mechanism.Methods The models of 70% warm ischemia-reperfusion injury were established in SD rats,rats were randomly divided into 4 groups: sham operation group,ischemia-reperfusion group,15d-PGJ2 group and 15d-PGJ2+GW9662 group.After reperfusion,serum AST and ALT levels were determined;the liver tissues were removed for measurement of activity of NF-?B and myeloperoxidase(MPO),TNF-? content and expression of ICAM-1.Results Compared with sham operation group,the serum levels of ALT and AST,and the activities of MPO and NF-?B,TNF-? content and expression of ICAM-1 in ischemia-reperfusion group,15d-PGJ2 group and 15d-PGJ2+GW9662 group were greatly improved(P

BACKGROUND:To construct tissue engineering cartilage would open up a novel way for the repair of cartilage damage in avoidance of the disadvantages of traditional therapeutic method.OBJECTIVE: To probe the techniques for the isolation of mesenchymal stem cells (MSCs) from bone marrow, as well as the in vitro differentiation into chondrocytic phenotype in a specific culture fluid.DESIGN:A complete randomized experimentSETTING:The Department of Traumatic Orthopedics and Hand Surgery of the First Affiliated Hospital of Guangxi Medical University, and Teaching and Research Faculty of Histology and Embryology of Guangxi Medical University.METHODS: The experiment was carried out at Guangxi Medical University between August 2002 and April 2003. Twenty SD neonatal weaning rats were selected. Bone marrow was aspirated from the bones of rat limbs and was isolated by gradient centrifugation in Percoll, and MSCs could be obtained in combination with adherent screening method, which were then cultured in DMEM-LG with 15% fatal bovine serum (FBS) in the incubator of 37℃ with 5% CO2 for 10-14 days. The passage cells were induced in DMEM-HG with 15% FBS (containing TGF-β1 10 μg/L, 10-7 mol/L dexamethasone, 50 mg/L VitC).MAIN OUTCOME MEASURES :The morphology, growth, as well as proliferation and specific expression of chondrogenic matrix of in vitro cultured MSCs due to specific induction.RESULTS: Totally 20 SD rats were observed and analyzed with no loss SCs grew in visible symmetric colonies, displaying a long-spindle shape,and the morphological characteristics of marrow-derived MSCs had no obvious changes during passage-culture, but its proliferation time was found from a shuttle fibroblastic appearance to polygonal shape, displaying posiHC staining of type Ⅱ collagen of cartilage specific matrix.bronectin adherent screening technique is a convenient, effective and practical method to separate and collect MSCs from rat bone marrows in chondrogenic phenotype when induced by a specific medium and can secrete cartilage specific matrix, and they can be the optimal seed cells for cartilage tissue engineering.

BACKGROUND:Transplantation of mesenchymal stem cells (MSCs) to multiple sclerosis (MS) is a new developed treatment to be given more and more attention.However,whether the MSCs function by cell replacement after going cross the blood-brain barricr or immune suppression needs further confirmation.OBJECTIVE:To establish a steady and effective method of Y chromosome in situ hybridization (ISH) and to detect the distribution of MSCs in a mouse model with MS.DESIGN,TIME AND SETTING:The observational study was performed at the Laboratory of Molecular Immunology,Institute of Healthy Science from September 2007 to February 2009.MATERIALS:A total of 30 C57BL/6 mice,aged 6-8 weeks and weighing 15-20 g,were selected.Female mice were used to establish experimental autoimmune encephalomyelitis (EAE).METHODS:Y chromosme specific DNA probe labled with digoxine (DIG) was designed and ISH was performed to confirm that the designed probe was Y chromosome specific and biologically sensitive.We transplanted the MSCs from male mice into the female EAE mice,and tracked the MSCs by Y chromosome ISH.MAIN OUTCOME MEASRUES:Distribution of MSCs in mice with EAE was observed under the optical and fluorescence microscope.RESULTS:The probe was confirmed to be Y chromosome specific and biologically sensitive.What's more,a steady and effective method of ISH was established.Some MSCs were detected in the periphery organs-spleen and lymph node of mice with EAE;seldom in spinal cord which indicated that MSCs might play its roles by immune suppression.CONCLUSION:Some MSCs were detected in the periphery organs-spleen and lymph node of mice with EAE,but seldom in spinal cord in the central nervous system.