This paper introduces the perioperative care of twelve chronic heart failure patients treated with cardiac resynchro-nization therapy,such as psychological care and correction of heart function during perioperative period, careful preparation of rescue instruments and observation of patients' conditions during the operation, intensive electrocardio monitoring,prevention of incision infection,nursing care of body position,and observation and treatment of postoperative complications during the postop-erative period. All the patients passed through the perioperative period safely and were discharged upon recovery. After a 1-6 months' follow-up,the patients' symptoms of heart failure was significantly improved and the exercise capacity was increased.

Abstract: The principles, detection limits, advantages, shortcomings and precautions for procedure of spectrophotometry, atomic absorption spectrometry, potential stripping analysis and osillo-polarography for determination of lead in environmental samples and biological materials were introduced in this paper to provide some reference data for the selecting suitable method for determination of lead based on the various kinds of samples, the samples sizes and the various conditions of analytical instruments and equipments.

Background Prevalence of dry eye is significantly inceasing in postmenopausal women than that in men,suggesting that sex hormone plays a role in the pathogenesis of dry eye.In addition,dry eye might become worse following estrogen therapy in postmenopausal women.However,whether application of androgen can ameliorate dry eye is being concerned.Objective This study was to investigate the effect of androgen on tear film of ovariectomized female rats.Methods Forty-eight 3-month-old sexually mature female Wistar rats were randomized into the normal control group,sham group,ovariectomy (OVX) model group and testosterone-injected group.OVX models were established by bilateral ovaries enucleation in the rats of the model group and testosterone-injected group,and then androgen (3.75 mg/kg) was intramuscularly injected since 5 months after OVX at 3-day interval for 6 weeks.Only intraperitoneal fat was cut off in the sham group.In 6 weeks after injection of androgen,serum androgen concentration detected and Schirmer Ⅰ test (S Ⅰ t),tear film break-up time (BUT) were performed.The rats were sacrificed to prepare the corneal and conjunctival samples.The expression of MUC5AC in conjunctival tissue was examined by immunofluorescence staining,and the microstructure of corneal cellular surface was observed under the scanning electron microscope before and 6 weeks after application of androgen.Animals in this study were treated in accordance with Animal Experimentation Ethic Committee Guidelines of Southern Medical University and the study protocol was approved by Ethic Committee of this University.Results The mean serum testosterone concentration was (1.83 ±0.12) ng/ml,and S Ⅰ t or BUT was (3.63-±0.26)mm/5 minutes or (3.73-0.38) seconds,respectively,in the OVX model group,which was significantly declined in comparison with (2.56 ±0.14) ng/ml,(7.47±0.66) mm/5 minutes or (9.57 ±0.76) seconds in the normal control group (all at P =0.000).However,the serum testosterone concentration was (3.38±0.24) ng/ml,S Ⅰ t was (6.37 ±0.45) mm/5 minutes and BUT was (7.54±0.55) seconds in the testosterone-injected group,which was significantly higher than that in the OVX model group (all at P =0.000).The positive staining of MUC5AC in rat conjunctival tissue weakened in the OVX model group compared with the normal control group,and the fluorescence intensity in the testosterone-injected group was stronger than that in the OVX model group.Regularly arranged microvilli and cell metabolism hiatus on the surface of corneal cells were seen in the normal control group and the sham group;while the microvilli were shorter and irregularly arranged,and the cell metabolism hiatus were disappeared in the OVX model group.However,microvilli and cell metabolism hiatus were close to normal ones in the testosterone-injected group.Conclusions Deterioration of tear secretion and instability of tear film are are probably associated with the lower serum androgen levels in castrated female rats.Systemic supplement of androgen can promote tear secretion,improve tear film stability and alleviate ocular surface damage.

Objective To study the effect of intracerebroventricular(icv) injectionon acetylcholine(ACh) on the elecric activities of pain-related neurons in the hippocampal CA1 area of rat.Methods Trains of the electric impulses applied to the right sciatic nerve were used as noxious stimuli.The discharges of neurons were led out by extracellular recording method with glass microelectrodes.Results Icv injection of ACh(20?g/10?l) caused an decrease of the evoked discharge frequency of pain-excited neurons(PEN) and a prolongation latency as well as an increase of the evoked discharge of pain-inhibited neurons(PIN) and a shortening of inhibitory duration.Conclusion The response of pain-related neurons in hippocampal CA1 area to the noxious stimulation is weakened by icv injection of ACh,exhibiting analgesic effect.

Objective To discuss specific CT signs of primary trachea,bronchus mucoepidermoid carcinoma.Methods A retro-spective analysis was made on CT features,clinical manifestations and relevant pathology materials of 10 cases of trachea,bronchus mucoepidermoid carcinoma that had been proved by pathological examinations.Results (1)Sites:1 case was in the trachea,1 case in the bronchus,3 cases in lobar bronchus,5 cases in segmental bronchus and they were all central types.(2)Form and size:2 cases were lobular,7 cases were oval or round,1 case was irregular.The maximum diameters of the tumor were 10-39 mm (averagely 25 mm).(3)Edge:8 cases had smooth edge,2 cases had rough edge with infiltration of adjacent pulmonary parenchyma.(4)Densi-ty:6 cases with uneven density,3 cases with even density.The density of 4 cases were higher than that of chest wall muscle,4 cases were similar to chest wall muscle and 1 case lower than chest wall muscle.Calcification can be seen in 4 tumors.(5 )Enhancement features:9 cases underwent CT enhancement scanning,8 cases had mild enhancement and 1 case with light enhancement.(6)Pe-ripheral conditions:all cases showed trachea or bronchus stenosis and blocking,5 cases with obstructive pneumonia,2 cases with obstructive atelectasis,3 cases with far-end bronchus obstructive mucous embolism,1 case with obstructive emphysema,there was no evident swelling in mediastinum or hilar lymph nodes,1 case with multiple pulmonary bulla and pneumothorax in the left lung. Conclusion Primary mucoepidermoid carcinoma of trachea and bronchus is mostly central type,low potential malignancy.CT mani-festations are as follows,occurred in the trachea or bronchus,oval or lobulated masses with smooth edge,slight enhancement,gen-erally with calcification,but few showed invasive growth .

Objective To evaluate the clinical effectiveness of percutaneous transhepatic gallbladder drainage introduced by bedside ordinary-probe-guidance with free-hand technique in severe cholecystitis. Methods 85 cases treated with the operation were observed and analyzed. Results Infectious bile was successfully drained in all cases without serious complications. 83 cases received satisfactory outcomes. 2 elderly patients died from MODS secondary severe infection. Conclusion Bedside ordinary-probe-guided free-hand percutaneous transhepatic gallbladder drainage is a safe and convenient treatment with minimal invasion for severe cholecystitis, which can be widely used in clinic.

Objective To explore the association between human papilloma virus(HPV)infection and antisperm antibodies(As-Ab)in male infertile patients in Xinjiang area.Methods A total of 127 cases of infertile male patients were selected as experimental group,other 130 cases of normal male were enrolled in the control group.HPV sperm infection was detected by using reverse dot blot hybridization kit.The levels of AsAbs in semen specimen were measured by using the enzyme-linked immunosorbent assay (ELISA).Results The infection rate of male infertile patients(67.7%)was higher than that of normal male(1 6.2%),with signifi-cant differences(P <0.05).The positive rate of AsAb of male infertile patients with HPV infection(38.4%)was higher than that of male infertile patients without HPV infection(1 7.1%),with significant differences(P <0.05).Compared with male infertile pa-tients without HPV infection,the percentages of sperm motility and grade (a+b)sperm motility were decreased in male infertile pa-tients with HPV infection,wihile the sperm malformation rate was increased in male infertile patients with HPV infection,with sig-nificant differences(P <0.05).Conclusion The HPV infection rate of male infertile patients is relatively high in this area.HPV sperm infection could be associated with AsAb that may further reduce male fertility.

Objective: To observe the effect of ATENS on distance and near vision and stereopsis in amblyopic children patients.Methods: 80 eyes from 70 patients with amblyopia were randomly divided into two groups: the group of ATENS and the group of western medicine.The difference of distance,near vision and stereopsis before and after treatment and between two groups after treatment were observed.Results: Distance and near vision improved and stereopsis diminished significantly after treatment in the TENS group and the western medicine group(P0.05).The curative effect and improving stereopsis in the western medicine group were better than that in the TENS group(P

Objective To explore the effect of the vitreoretinal surgery on the complicated retinal detachment.Methods We collected the clinical data of 50 cases with complicated retinal detachment treated by vitreoretinal surgery combined with the methods of vitrectomy,membrane excision,perfluorocarbon liquids,retinal coagulation of laser or condensate,exchange between silicon oil and gas perfluorocarbon liquids,silicon oil fill.The period of follow up was 3 to12months.Results The retina was attached completely in 46 cases(92%).4 cases did not reset auownting for 8%.The sight of all eyes was improved in varying degrees.Conclusion Vitreoretinal surgery is an effectial way for treat complicated retinal detachment.

To explore the effects of two breathholding and exerting methods on the second labor course of primiparas.Methods Six hundred primiparas were divided according to the admission time into two groups: the control group and observation group. The former took conventional breathholding and exerting method and the latter breathholding and exerting method with breathing techniques. The two groups were compared in terms of outcome of delivery, rate of episiotomy, time for breathholding and exerting and the hemorrhage volume 2h after delivery, perineal laceration and neonate asphyxia.Results The rate of spontaneous delivery in the observation group was higher than that in the control group. The rate of episiotomy, bleeding volume 2h after delivery and perineal laceration were lower. The breathholding time was significantly shorter(P<0.05).Conclusion Breathholding and exerting at the second stage of labor can reduce energetic consumption and improve the outcome of delivery.