Objective To determine the role of valproate in the expression of SCN3A and provide new evidence for the clinical treatment of epilepsy. Methods We treated the N1E-115 cells with sodium valproate and examine the expression of SCN3A mRNA. We also constructed the expression vector of the SCN3A gene promoter then transfected the vector into N1E-115 cells ,respectively detected the level of SCN3A mRNA and the expression level of firefly luciferase of N1E-115 cells with valproate treated and untreated. Results Valproate inhibited the expression of SCN3A significantly including the level of SCN3A mRNA and luciferase protein. Conclusion Valproate inhibits the seizures by reducing the expression of the SCN3A, which provides a new understanding of the treatment of epilepsy.

Objective To discuss the effect of the modified structure of oxygen equipment on oxygen inhalation effect of patients receiving oxygen therapy.Methods The structure of oxygen equipment was modified.and 80 patients who required oxygen therapy were divided into the modified group and the non-modified group according to odd and even number of hospital admission sequence randomly with 40 patients in each group.The modified group used the modified oxygen equipment,the non-modified group used traditional one.The differences of respiration,heart rate and blood oxygen saturation rate between the two groups before oxygen inhalation,30 minutes and 2 hours after oxygen were compared.Results There was no significant difference in the respiration,heart rate and blood oxygen saturation rate between the two groups.Conclusions The modified oxygen equipment doesn't affect the oxygen therapy effect of patients,besides,it avoids the problems of pollution and the complicated disinfection procedure of the structure of the humidifier bottle,fluid and breathing vessel of the traditional equipment.

Objective To assess radiation dose and image quality of chest CT examinations that acquired with Flash scan technique combined with iterative reconstruction.Methods Thirty continuous patients required no-contrast chest CT with Flash scan mode and contrast enhanced chest CT with conventional CT scan mode.For each examination,the images were reconstructed by filter back projection (FBP) and sinogram affirmed iterative reconstruction (SAFIRE).The volume CT dose index (CTDIvol),dose-length product (DLP),and effective dose (ED) were estimated.The image noise,signal to noise ratio (SNR),overall subjective image quality,and respiratory motion artifacts were evaluated blindly by two radiologists.Results For Flash scan and conventional scan,SAFIRE reduced the image noise 35％ and 16％,respectively;and increased SNR 21.9％ and 25％ compared to FBP,respectively (P ＜0.05).There was significant lower subjective image scores acquired by SAFIRE than by FBP.Flash scan reduced the CTDIvol (mGy) 40.9％,DLP 32.1％,and ED 26.9％ compared to conventional CT scan mode.The motion artifact scores were reduced significantly with Flash scan mode compared to conventional scan mode.Conclusions Flash scan technique of the chest can obtain image with minimum respiratory motion artifact due to dramatically increased scan speed,and when SAFIRE was combined with,it reduces significantly radiation dose and image noise.

Objective: To prepare the compound Yuxingcao dispersible tablets and investigate the dissolution of baicalin in vitro. Methods: The formulation of dispersible tablets was optimized by an orthogonal design test in terms of disinterating time.Results: The optimal disintegrants were composed of CMS-Na 5%,MCC 20%,L-HPC 10%,and disintegration time of prepared compound Yuxingcao dispersible tablets followed the quality specification of dispersible tablet in Chinese Pharmacopoeia 2005. The in vitro dissolution test indicated that more than 95% of baicalin dissolved in 3 minute from dispersible tablets. Concolusion: The formulation and preparation process of compound Yuxingcao dispersible tablets were simple and feasible.

Objective To study the distribution of the sulfur dioxide (SO2) in the different organs of mice after exposed to SO2. Methods The Kunming mice were exposed to SO2 at different doses in a chamber in which the concentration of SO2 could be monitored. The concentrations of sulfite in livers, kidneys, spleens and testis from male mice were determined by high performance liquid chromatography (HPLC) with fluorescence detection (FD). After reduction, pre-column derivation to tissue homogenates of livers, kidneys, spleens and testis, the mixture was centrifuged, and 5 ?l of the resulting supernatant was directly injected into HPLC with analytical column C18(200 mm ? 5.0 mm, 5 ?m), where the mobile phase consisted of a mixture methanol-water(contained 0.25% acetic acid ) (10:90,V/V), pH was 2.8 adjusted with phosphoric acid, and the exciting and emitting wavelength of fluorescence detection were 392nm and 479nm,respectively. The mobile phase was pumped at a flow rate of 1.0 ml/min, and the column temperature was 30 ℃. Results The standard curve of sulfite was linear in the range from 0.063 ?g/ml to 1.26 ?g/ml (r=0.997 8), and the minimal detectable concentration was 0.05 ?g/ml (S/N=3) with a recovery rates of 95%-101%. The relative standard deviations (RSD) of intra-day and inter-day RSD were less than 10%. The results showed that sulfite concentration in all organs tested from mice in SO2-exposed groups was significantly increased (P

Objective To investigate the protective effect of Limb ischemic postconditioning ( LIPostC) on blood brain barrier (BBB) and explore the influence on the the expression of tight junction protein claudin-5,occludin in cerebral ischemia-reperfusion injury of mice.Methods Ninety male CD1 mice were divided into three groups randomly:Sham-operated group(sham group), ischemia-reperfusion group(I/R group) and LIPostC group.Mouse middle cerebral artery cerebral ischemia-reperfusion model was established by modified-occlusion method. Neurological deficit scores, brain water content, infarct volume, BBB dysfunction were measured at 24 h after reperfusion.Western blot and RT-qPCR were used to analyze the expressions of claudin-5, occludin.Results Compared with I/R group, neurological deficits scores were significantly decreased, brain edema was relieved, infarct volume was reduced, and the expression of claudin-5, occludin up-regulated, the differences were statistically significant(all P<0.05).Conclusion LIPostC can protect the BBB of cerebral ischemia-reperfusion injury mice by increasing the expression of claudin-5,occludin, and that will provide a theoretical basis for its clinical application.

Objective To explore the effects of family and telephone follow-up on quality of life of the patients with tota1 hip arthroplasty(THA).Methods One hundred and twelve patients with THA were randomly divided into experiment group and control group with 56 cases in each group.The experiment group was treated with rehabilitative instruction by family and telephone follow-up.The control group was treated with outpatient follow-up after leaving the hospital.The living quality in both groups were compared by the medical outcomes study 36-item short-form health survey(SF-36 QOL)one year after leaving the hospital.Results One-year after leaving the hospital,the total score and demension score of experiment group were better than those of the control group.There was significant difference between the control group and in experiment group(P<0.05).Conclusion Family and telephone follow-up is an effective implementation for THA patients to accomplish physiological functional recovery and promote the quality of life.

Abstract: The principles, detection limits, advantages, shortcomings and precautions for procedure of spectrophotometry, atomic absorption spectrometry, potential stripping analysis and osillo-polarography for determination of lead in environmental samples and biological materials were introduced in this paper to provide some reference data for the selecting suitable method for determination of lead based on the various kinds of samples, the samples sizes and the various conditions of analytical instruments and equipments.

[Objective]To discuss the changes and clinical significance of microcirculation in lower limbs in patients with lumbar canal stenosis under different circumstances.[Method]Prospectively,randomly,the nail fold microcirculation of the big toe in 50 patients were investigated with WX-6 microcirculation instrument before and after operation at 7~10 days and 3~6 months.The averages were compared with that of 30 healthy persons with q test.Two groups were defined according to the claudication distance as 0～100 meter and 300～400 meter and tested.The averages were analyzed with t test.[Result]There were statistically significant differences between the patients group and the healthy group and before and after surgery(P0.05).The most significant change was the form of blood flow.[Conclusion]The dysfunction of sympathetic nerve resulting from lumbar canal stenosis will impair the microcirculation of lower limbs and aggravate intermittent claudication.The operation will improve the microcirculation and alleviate the symptoms significantly.The microcirculation test in lower limbs can serve as an objective method for the preoperative diagnosis and postoperative assessment for therapeutic efficacy.

In this paper, the preliminary study on antioxidant, enhancement of antioxidant enzymes activity, reducing the content of oxygen free radicals, delaying skin aging of the recombination cytoglobin (rCygb) purified by our lab were investigated through human keratinocyte cell line (HaCAT) H2O2 oxidative stress model, mouse skin aging model caused by continuous subcutaneous injection D-gal, rat acute liver injury model induced by CCl4 and rat skin wound healing model. The results showed that rCygb improved the activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), reduced the activities of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) as well as decreased the content of malondialdehyde (MDA). Skin biopsy showed that rCygb promoted angiogenesis, increased expression of collagen and improved the anti-inflammatory ability. All results displayed that rCygb improved the oxygen free radical scavenging ability, delayed skin aging and promoted wound healing.