1.RP-HPLC Determination of Matrine in Fuji Lotion
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(02):-
Objective To establish a method for determination of matrine in Fuji Lotion. Methods An Inertsil NH2 column (250 mm?4.6 mm, 5 ?m) was used with acetonitrile-ethanol absolute-3% phosphoric acid (83∶8.5∶8.5) as mobile phase, the flow rate was 1.0 mL/min, and the UV detection wavelength was set at 220 nm. Results The calibration curve of matrine was linear in range of 0.039~1.560 ?g, r=0.999 7. The average recovery was 97.8% with RSD of 1.1% (n=6). Conclusion The method is simple, quick and accurate. It can be used for the quality control of Fuji Lotion.
2.Treatment of fungal rhinosinusitis with endoscopic sinus surgery: A report of 19 cases
Chinese Journal of Minimally Invasive Surgery 2001;0(06):-
Objective To evaluate the effect of endoscopic sinus surgery (ESS) for treating fungal rhinosinusitis. Methods A total of 19 patients with fungal rhinosinusitis were treated under endoscope in this hospital from January 2000 to December 2003. In operation, the polyps or granulations in the middle nasal meatus were cleared away. Then after the removal of the uncinate process, all sinus ostiums were enlarged to further get rid of pathological tissues in the sinus. During the surgery the sinus was repeatedly irrigated with 3% H_2O_2. If the drainage of the sinus ostium had been affected by the narrowing of the middle meatus resulted from a deviation of the nasal septum, a synchronal correction of the nasal septum was carried out. Results Symptoms remitted in 5 patients and disappeared in 14 postoperatively. Follow-up for 6~48 months (mean, 14 months) in 19 patients found 2 patients with recurrence, which was re-operated on with the Caldwell-Luc procedure. No complications were observed. Conclusions The ESS is effective in the treatment of non-invasive fungal rhinosinusitis. However, this technique cannot replace the Caldwell-Luc procedure in the management of serious fungal maxillary sinusitis.
3.Influence of hypoxic preconditioning on liver cancer hepatectomy
Pengfei LI ; Peijian ZHANG ; Xiaofan HAN
International Journal of Surgery 2012;39(7):478-480
Hypoxic preconditioning improves organism in stress response such as operation.This dissertation expound the effects of hypoxic preconditioning on hepatic carcinoma after hepatic resection.Hypoxic preconditioning protect remainder hepatocyte from ischemia reperfusion injury in the remaining liver by regulating HIF-1,nuclear factor-kappa B and being kind to our mitochondria; remote-ectopic hypoxic preconditioning and cross-pluripotential hypoxic preconditioning preserve residual liver.Hypoxic preconditioning can relieve liver from ischemia reperfusion injury after hepatocellular carcinoma hepatectomy,but no clear evidence demonstrate it improve residual liver regeneration.This need further experimental study.
4.Influence of comprehensive rehabilitation training on the cognitive impairment of chronic schizophrenics
Weihong ZHANG ; Xiaofan WU ; Jingli GAN
Medical Journal of Chinese People's Liberation Army 1983;0(02):-
Objective To study the therapeutic effects of comprehensive rehabilitation training on cognitive impairment of chronic schizophrenics.Methods 86 inpatients with chronic schizophrenia were randomly assigned to the observation group(n=42)and the control group(n=44).They were studied with the Event-related potential P300 and the Positive and Negative Syndrome Scale(PANSS)before and after comprehensive rehabilitation training.Results Compared with controls,the observation group manifested shorter latency and higher amplitude on Target P3 wave of P300 after treatment(P
5.Comparison of the effects of penequinine hydrochloride and atropine on the cardiovascular stability about preoperative in children
Peng ZHANG ; Gang LIU ; Xiaohui PENG ; Qijun XIONG ; Xiaofan ZHANG
Chinese Journal of Primary Medicine and Pharmacy 2009;16(6):1017-1018
Objective To compare the effects of penequinine hydrochloride and atropine on the cardiovascu-lar stability about preoperative in children by monitoring and recording the HR, MAP, SpO2, ECG changes. Methods 40 children underwent selective operation were randomly divided into two groups, 20 in each group. Penequinine hydrechloride group(group A) and atropine group(group B) were injected intramuscularly with penequinine hydro-chloride 0. 015mg/kg or atropine 0. 015mg/kg respectively, at 30 minutes before operation. Patients' HR, MAP, SpO2 and ECG changes were recorded at 10min,20min,30min and 40min after injection. Results The HR,MAP,SpO2 levels of group A and SpO2 level of group B had no significant changes. There are significant changes in HR,MAP of group B. Conclusion Penequinine hydrochloride can be used as preoperative medication and it is conducive to the maintenance of cardiovascular stability.
6.Survivin-siRNA inhibits proliferation of lung cancer A549 cells and enhances their chemosensitity to cisplatin
Shuguang ZHANG ; Xiaofan LIU ; Jiang DU ; Wenya LI ; Lin ZHANG
Chinese Journal of Cancer Biotherapy 2009;16(6):583-587
Objective:To investigate the effect of survivin-siRNA plasmid on survivin expression in human lung cancer cell line A549, and to observe its effect on the apoptosis, proliferation, and chemosensitivity of A549 cells. Methods: pSilencer-survivin-siRNA (survivin-siRNA) plasmid was constructed using pSilencer-U6 plasmid and was transfected into A549 cells. Expression of survivin mRNA and protein was examined by RT-PCR and Western blotting analysis, respec-tively. Apoptosis and proliferation of A549 cells were examined by DAPI staining and MTT, respectively. Results: Sur-vivin-siRNA plasmid was successfully constructed, and it significantly inhibited survivin mRNA and protein expression in A549 cells. Survivin-siRNA transfection induced apoptosis, inhibited proliferation and increased chemosensitivity of A549 cells to cisplatin. Conclusion: pSilencer-survivin-siRNA can silence survivin expression in A549 cells and subsequently inhibit proliferation, promote apoptosis, and enhance chemosensitivity of A549 cells to cisplatin. Survivin may serve as a potential target for gene therapy of lung cancer.
7.Prognostic value of procalcitonin in patients with acute paraquat intoxication
Xiaofan ZHANG ; Li LI ; Chao LAN ; Li HUANG ; Guoyu DUAN
Chinese Journal of Emergency Medicine 2016;25(8):1044-1048
Objective To study the prognostic value of procalcitonin (PCT) level in the outcome of patients with paraquat poisoning (PQ).Methods The clinical data of 128 patients with acute PQ admitted to emergency department were collected from March 2013 through March 2014.The patients were divided into two groups:the death group and the survival group (survival of 28 days).Poisoning doses,urine concentration of PQ,time elapsed from poisoning to admission,and time elapsed from poisoning to gastrolavage were documented.And on the 1 st day,the 3rd day and the 7th day after poisoning,serum PCT were detected.The level of PCT was used to investigate the prognostic values in patients with acute PQ in the death group and survival group.Results Of 128 cases,72 (56.3%) survived and 56 died in 28 days.Among them,the level of PCT increased to some extent in the first day in 90 cases,and 48 patients died.According to trend analysis,the levels of PCT in death group on the 1st day,the 3rd day and the 7th day after PQ were significantly higher than those in survival group [ld:(0.96 ±0.13) vs.(0.08 ±0.01),3d:(1.12 ±0.14) vs.(0.28 ±0.05),7d:(1.22 ±0.14) vs.(0.20 ±0.03),P <0.01].There was a trend of escalating PCT levels in death group,whereas the PCT level reached the peak on the 3st day and decreased gradually in the following days in survival group.The early PCT level was obviously related to poisoning doses,urine concentration,CRP,WBC,ALT,CR (the coefficient of association were 0.794,0.723,0.724,0.332,0.700,0.414,respectively,P<0.01).Conclusions The serum level of PCT increased in patients with acute PQ was significantly positively correlated with the oral dose and urine concentration of paraquat,and it can be used as an indicator for PQ severity.There is important clinical significance in detecting the change of serum level of PCT for estimating the condition of patients and evaluating the prognosis.
8.Effects of intravenous immunoglobulin on immunity and cytokine levels in children with EV71 infection associated high-risk pulmonary hemorrhage
Peng LI ; Chunlan SONG ; Yibing CHENG ; Yajie CUI ; Xiaofan ZHANG
The Journal of Practical Medicine 2016;32(19):3219-3222
Objective To investigate the influence of IVIG on immunologic function and cytokines levels in children with EV71 infection associated high-risk pulmonary hemorrhage. Methods According to the inclu-sion criteria , 64 children were enrolled and randomly divided into two groups: 39 cases in the IVIG treatment group and 25 cases in the general treatment group. The alternations of blood and immune cytokine markers before and after treatment were detected in the patients. Results (1) Before treatment, the peripheral blood T cells, TH and B cells in the IVIG group were higher than those in the general group , but the peripheral blood IgA was lower than that in the general group(P < 0.05); (2) In the IVIG group, the NK cells and IgG increased and T cells, TH cell and B cells significantly decreased after receiving IVIG therapy (P < 0.05); (3)No significant difference was observed in the levels of IL-6 and IL-10 in the two treatment groups before treatment. However , after IVIG treatment, IL-6 and IFN-γ levels reduced, IL-10 level increased (P < 0.05). After the general treat-ment, no significant difference was found in levels of IL-6,IL-10 and IFN-γ in peripheral blood (P > 0.05). Conclusion Disorders of cellular immunity and humoral immunity appeared in children with EV71 infection-re-lated high-risk pulmonary hemorrhage. It has clinical value to use IVIG timely to regulate the immune disorder.
9.Characteristics of CD8+ stem memory T cells in patients with chronic HIV-1 infection during antiretroviral therapy
Xiaofan LU ; Huan XIA ; Bin SU ; Hao WU ; Tong ZHANG
Chinese Journal of Microbiology and Immunology 2017;37(4):250-254
Objective To investigate the characteristics of CD8+ stem memory T cells (CD8+Tscm) in patients with chronic HIV-1 infection before and after antiretroviral therapy (ART) and to analyze their associations with progression of HIV-1 infection.Methods Thirty-six patients with chronic HIV-1 infection and 20 healthy subjects were enrolled in this study.Flow cytometry was performed to detect the percentages and absolute numbers of CD8+Tscm in patients with chronic HIV-1 infection before and after antiretroviral therapy (ART) as well as in healthy subjects.Correlation analysis was used to demonstrate the relationships between CD8+Tscm and markers for progression of HIV-1 infection (CD4+T cell count, HIV-1 viral load and level of activated T cells).Results The percentages and the absolute numbers of CD8+Tscm in patients with chronic HIV-1 infection had no significant change before and after ART.They were respectively positively correlated with the percentages and the absolute numbers of CD4+Tscm.The percentage of CD8+Tscm was proportional to the percentage of CD8+ central memory T cells (CD8+Tcm), but was inversely proportional to the percentage of CD8+ effector memory T cells (CD8+Tem).In addition, the percentages of CD8+Tscm in patients with HIV-1 infection were negatively correlated with the viral loads before ART.Conclusion CD8+Tscm are responsible for maintaining the homeostasis of other CD8+T cell subsets.CD8+Tscm play an important role in inhibiting viral replication.
10.Application of prospectively electrocardiograph-gated 128-slice spiral CT angiography in children with complex congenital heart disease
Xiaofan ZHANG ; Lihong ZENG ; Zhiwei WANG ; Fang WANG
Chinese Journal of Radiology 2013;47(8):739-744
Objective To evaluate the prospective electrocardiograph (ECG)-gated 128-slice spiral CT angiography in the diagnosis of complex congenital heart disease and airway abnormality in children.Methods Eighty children with congenital heart disease received prospective (n =40) or retrospective ECG-gated 128-slice spiral CT angiography (n =40).All of the patients underwent ultrasound cardiogram (UCG) within one week before CTA exmination.The image quality and the effective radiation dose were statistically analyzed using independent samples t test and x2 test.The surgical results were taken as the diagnostic standards,and the preoperative prospective and retrospective CT angiography data were compared with UCG results using x2 test.Results The effective dose of prospective ECG-gated CT angiography[(1.00 ± 0.26)mSv] was reduced by 86% as compared with the retrospective ECG-gated CT angiography[(7.37 ± 0.52) mSv] (t =69.296,P < 0.01).Image quality was graded 0 in none patients,graded 1 in 2 patients who underwent prospective ECG-gated CT angiography,graded 2 in 8 and 1 patient who received prospective and retrospective ECG-gated CT angiography respectively,and graded 3 in 30 and 39 patients who underwent prospective and retrospective ECG-gated CT angiography,respectively.There was no significant difference in image quality between prospective and retrospective ECG-gated CT angiography (x2 =0.044,P > 0.05).The definitive diagnosis of extracardiac vascular anomalies was made in 98.3%(59/60) of patients by using prospective and retrospective ECG-gated CT angiography,which were significantly superior to UCG results[(76.7% (46/60),76.7% (45/59),respectively] (x2 =13.144,12.644,P < 0.01),and there was no significant difference in the definitive diagnosis between prospective and retrospective ECG-gated CT angiography (x2 =0.001,P > 0.05).The definitive diagnosis of the cardiac/large-vessel malformations was made in 100% of the patients by both prospective and retrospective ECG-gated CT angiography,and there was no significant difference between 128-slice spiral CT angiography results and UCG results [97.2% (35/36),97.3% (36/37),respectively] (x2 =1.014,0.001,P >0.05).In the cardiac malformations,the definitive diagnosis by prospective and retrospective ECG-gated CT angiography was 88.7 % (47/53) and 92.5 % (49/53),and there was no significant difference between 128-slice spiral CT angiography results and UCG results [98.1% (52/53),98.1% (52/53),respectively](x2 =3.824,1.889,P > 0.05),and no significant difference in the definitive diagnosis was observed between prospective and retrospective ECG-gated CT angiography (x2 =0.442,P > 0.05).In addition,the 128-slice spiral CT angiography detected airway abnormality in 9 cases who underwent prospective or retrospective ECG-gated CT angiography.Conclusion The prospective ECG-gated 128-slice spiral CT angiography exhibits great application value in the preoperative diagnosis of complex congenital heart disease,which helps to develop surgical program and evaluate surgical risks.