The purpose of this paper is to present the research on the molecular biological characteristics of proto-oncogene pim-2 and to analyze the related mechanism. Proto-oncogene pim-2 was studied and analyzed by the bioinformatics method and technology. With an online server, the chromosomal localization of pim-2 gene was analyzed, and the exon, open reading frame, CpG island and miRNAs complementary fragments and the like were predicted. With bioinformatics software, the physicochemical property of transcription protein of proto-oncogene pim-2 and various modification sites of protein sequence, such as ubiquitination and glycosylation, were predicted, the antigenic index was calculated, and the spatial structural was modeled. The research findings showed that the proto-oncogene pim-2 comprised six exons, the CDS (coding sequence) transcribed a section of peptide chain including 311 amino acids, a gene promoter has a CpG island, and the 3'UTR region contains an miRNA gene. The molecular weight of the Pim-2 protein was 34,188. 47, the isoelectric point was 5.78, the instability index was 45.87, and the extinction coefficient was 279nm. A plurality of covalent modification sites, two ubiquitination sites, four glycosylation sites, an SUMO sumoylation site, a nitrosation site, two palmitoylation sites and sixteen regions with higher antigenic index were distributed in the protein sequence. This research showed that the related regions and modification sites distributed on the sequence of proto-oncogene pim-2 were closely related to the carcinogenic effect thereof.
Amino Acid Sequence ; CpG Islands ; Exons ; Open Reading Frames ; Protein-Serine-Threonine Kinases ; genetics ; Proto-Oncogene Proteins ; genetics

Objective To explore the clinical behavior,pathological and immunohistochemical characteristics and diagnosis, treatment methods of gastrointestinal stromal sarcomas (GISS). Methods Retrospective analysis was perfomed over 36 GISS patients based on clinical data and pathological data. Results GISS occurred more in the stomach and small intestine, than colon and rectum. Immunohistochemical staining showed CD117, CD34 expression,the positive rates were 91.7% and 86. 1%. Conclusion GISS is a rare malignant tumor, GISS diagnosis depends on the histologic and immunohistochemical test. CD34 and CD117 in the positive diagnosis has its special significance. Surgery is the main treatment.

OBJECTIVE:To investigate the role of clinical pharmacists in pediatric severe infection treatment,and to provide reference for rational drug use in clinic. METHODS:Based on related typical cases,the breakthrough points of clinical pharmacists providing pharmaceutical care in pediatric severe infection treatment plan were introduced. RESULTS:Clinical pharmacists formu-lated multi-drug resistance pathogenic antibacterial plan according to the results of pathogenic microbiological examination,use pharmacokinetics/pharmacodynamics,excluded interference to pediatric anti-infection treatment from ADR,and guideline and eco-nomical factors of patients. CONCLUSIONS:Clinical pharmacists adopt professional knowledge of pharmacy to provide pharmaceu-tical care in pediatric severe infection treatment and play professional assisted role so as to guarantee safe,effective and economical treatment.

Fracture healing activity of aqteous extracts of rhizome of Drynaria fortunei (Kunze ) J.Sm.,D.sinica Diels ( "Gusuibu"),and its main constitucnt naringin were examined and coufirmedwith an experimental bone-injury model rat femur inferior. Results showed that they werefefective for healing of experimental rat bone-injury.

A non-linear rectification based on immune genetic algorithm (IGA) is proposed in this paper, for the shortcoming of the non-linearity rectification. This algorithm introducing the biologic immune mechanism into the genetic algorithm can restrain the disadvantages that the poor precision, slow convergence speed and early maturity of the genetic algorithm. Computer simulations indicated that the algorithm not only keeps population diversity, but also increases the convergent speed, precision and the stability greatly. The results have shown the correctness and effectiveness of the method.
Algorithms ; Computer Simulation ; Immunity ; genetics

Objective To evaluate the efficacy and safety of brachytherapy by percutaneous paracentesis implantation of iodine-125 seeds for elderly non-small cell lung cancer.Methods 18 patients with peripheral lung cancer were undergone brachytherapy by percutaneous paracentesis implantation of iodine-125 seeds.The effect was evaluated by periodically chest CT.The complications were analyzed.Results 18 patients were observed one,two and six months after operation.CR,PR,SD were seen in 27.8％(3/18),66.7％(14/18),5.6％(1/18)respectively of the patients.Pneumothorax,hemoptysis were seen in 16.67％(3/18),95.37％(17/18),no pleural cavity bleeding.Conclusion Brachytherapy by percutaneous paracentesis implantation of iodine-125 seeds for lung cancer was effecitve.The complications were easy to treat.The methods of implantation of iodine-125 seeds could fully play the advantages of CT,and reduce the damage to the body when raising the tumorous control rate.

Objective To observe the effects of different posture on hemodynamics and plasma atrial natriuretic polypeptide(ANP) during laparoscopic surgery. Methods Forty patients who scheduled for elective laparoscopic surgery under general anesthesia were allocated into two groups according to their posture during laparoscopic surgery,20 cases for each group. In group A, the patients were arranged in a head-down tilt position, in group B, the patients were arranged in a head-up tilt position systolic blood pressure (SBP),diastolic blood pressure (DBP),central venous pressure (CVP) and electro cardio gram (ECC) were monitored continuously. Blood samples were taken from central venous at four time points of prepneumoperitoneum(T1), 10 minutes after that(T2) and 20 mintues(T3) when the patients were arranged at the different operation-needed position with a stable pneumoperitoneum pressure of 14 mm Hg (1 mm Hg = 0.133 kPa),and at 5 minutes (T4) after deflation of pneumoperitoneum when the patients returned to supine position. The plasma ANP was assessed by radioimmunoassay. Results In group A,the CVP at T2 and T3 [(14.45 ±2.72),(14.20 ±2.46) mm Hg] was significantly higher than that at T1 [(6.05 ±1.76) mm Hg] (P<0.01), in group B,the CVP at T2 and T3 [(8.90±1.27),(9.02 ±0.47) mm Hg] was significantly higher than that at T1[(6.30 ±1.34) mm Hg](P< 0.01) ,with a higher level in group A than those in group B at the same time point during pneumoperitoneum(P< 0.01). The ANP level in group A was higher at T2 than that at T1, and there was significantly higher at T3 than that at T1 (P < 0.05). But the ANP level was significantly higher in group A than that in group B at the same time points of T2 and T3 (P < 0.05). Conclusion The posture may have obvious effect on CVP and plasma ANP level during laparoscopic surgery.

Objective To study the effect of climate change on physiological indexes of elderly people.Methods From January,2006 to January,2007,30 elderly couples were selected and blood pressure,pulse,body weight were investigated.The weather data was collected.Results The lowest value of body weight appeared in February in normotensive subjects,in September in hypertension subjects.The lowest value of body temperature in two groups appeared in February and March.The lowest value of pulse appeared in March and April in normotensive subjects,in September in hypertension subjects.The curve of blood pressure change(SBP and DBP) presented"V"in two groups.The lowest value appeared in summer(July-September).The highest value appeared in winter(December-February).The blood pressure in winter was higher than that in summer(P

Forty patients with pulmonary sclerosing hemangioma treated from 1971 to 1999 in General itospital of PLA were analyzed retrospectively for clinical and pathological features,and image characteristics. The results showed that 60%(24 of 40) of patients were middle aged or elderly women,and 40% (16 of 40) of patients had symptoms.On chest radiography,the tumor presented as a round or nearly round nodule or mass with sharp and smooth margin in 37 cases(92 5%).All patients received operation without complication or mortality.Immunohistochemical findings indicated that the tumor cells were positive for neuroendocrine markers,and neurosecretory granules were found in tumor cells by electron microscopy.There was no recurrence or metastasis at the follow up. It suggested that pulmonary sclerosing hemangioma is a benign neuroendocrine tumor that has a good prognosis if it is treated by operation.

To evaluate whether different doses of X ray irradiation can induce apoptosis of rat C6 glioma cells. A rat C6 glioma cell was irradiated with X ray in different doses in vitro. Apoptosis was identified by the classical “ladder” pattern (oligonucleosome sized fragments) in DNA agarose gel electrophoresis. Results: The results showed that significant apoptosis was found in 15, 20 or 30 Gy groups at 4 hours and 1 day after X ray irradiation compared with control, whereas this phenomenon could only be seen vaguely in the 40 Gy group. The latter group showed more necrosis than apoptosis. It suggested that the apoptotic mode of cell death and necrosis may represent the response in this X ray irradiated rat C6 glioma cells in vitro.